TY - JOUR AU - Acsády, László AU - Katona, István AU - Gulyás, Attila AU - Shigemoto, R AU - Freund, Tamás TI - Immunostaining for substance P receptor labels GABAergic cells with distinct termination patterns in the hippocampus JF - JOURNAL OF COMPARATIVE NEUROLOGY J2 - J COMP NEUROL VL - 378 PY - 1997 IS - 3 SP - 320 EP - 336 PG - 17 SN - 0021-9967 DO - 10.1002/(SICI)1096-9861(19970217)378:3<320::AID-CNE2>3.0.CO;2-5 UR - https://m2.mtmt.hu/api/publication/107901 ID - 107901 N1 - Megjegyzés-27029437 Megjegyzés-21894930 Z9: 53 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Katalin AU - Freund, Tamás AU - Miles, R TI - Disinhibition of rat hippocampal pyramidal cells by GABAergic afferents from the septum JF - JOURNAL OF PHYSIOLOGY-LONDON J2 - J PHYSIOL-LONDON VL - 500 PY - 1997 SP - 463 EP - 474 PG - 12 SN - 0022-3751 DO - 10.1113/jphysiol.1997.sp022033 UR - https://m2.mtmt.hu/api/publication/107892 ID - 107892 N1 - Lab. de Neurobiologie Cellulaire, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1450 Budapest, Hungary Cited By :325 Export Date: 3 August 2023 CODEN: JPHYA Correspondence Address: Miles, R.; Lab. Neurobiologie Cellulaire, 25 rue du Dr Roux, 75724 Paris cedex 15, France; email: rmiles@pasteur.fr Chemicals/CAS: acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; cesium chloride, 7647-17-8; picrotoxin, 124-87-8 LA - English DB - MTMT ER - TY - JOUR AU - Acsády, László AU - Arabadzisz, Dimitrula AU - Freund, Tamás TI - Correlated morphological and neurochemical features identify different subsets of vasoactive intestinal polypeptide-immunoreactive interneurons in rat hippocampus JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 73 PY - 1996 IS - 2 SP - 299 EP - 315 PG - 17 SN - 0306-4522 DO - 10.1016/0306-4522(95)00610-9 UR - https://m2.mtmt.hu/api/publication/107952 ID - 107952 N1 - Cited By :160 Export Date: 1 August 2023 CODEN: NRSCD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, P.O. B. 67, H-1450 Budapest, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; parvalbumin, 56094-12-3, 83667-75-8; vasoactive intestinal polypeptide, 37221-79-7 Funding details: Howard Hughes Medical Institute, HHMI Funding details: Human Frontier Science Program, HFSP Funding details: Hungarian Scientific Research Fund, OTKA, 5532 Funding text 1: The possible functional roles of VIP-containing interneuron types revealed in this study may be further elucidated by identifying their postsynaptic elements, which is the subject of the accompanying paper. 1 Acknowledgements--We are grateful to Dr T. Grrcs for antisera against VIP and CCK, to Dr M. R. Celio for anti-parvalbumin and anti-calbindin and to Drs J. H. Rogers and I. Virtanen for antisera against calretinin and GABA, respectively. The excellent technical assistance of Ms E. Bor6k, Ms I. Weisz and Ms A. Zrldi is also acknowledged. This work was supported by the Human Frontier Science Program Organization, the Howard Hughes Institute and OTKA (No. 5532) Hungary. LA - English DB - MTMT ER - TY - JOUR AU - Acsády, László AU - Görcs Tamás, Jenő AU - Freund, Tamás TI - Different populations of vasoactive intestinal polypeptide-immunoreactive interneurons are specialized to control pyramidal cells or interneurons in the hippocampus JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 73 PY - 1996 IS - 2 SP - 317 EP - 334 PG - 18 SN - 0306-4522 DO - 10.1016/0306-4522(95)00609-5 UR - https://m2.mtmt.hu/api/publication/210681 ID - 210681 N1 - Megjegyzés-27028766 Megjegyzés-21894987 Z9: 130 Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, Budapest, H-1450, Hungary First Department of Anatomy, Semmelweis University, Medical School, Budapest, H-1450, Hungary Cited By :211 Export Date: 21 August 2019 CODEN: NRSCD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, H-1450 Budapest, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; vasoactive intestinal polypeptide, 37221-79-7 Funding details: Human Frontier Science Program Funding details: Howard Hughes Medical Institute Funding details: Hungarian Scientific Research Fund, T16962, T5532 Funding text 1: Acknowledgements We are grateful to Dr P. Somogyi for antisera against GABA and to Dr K. G. Baimbridge for antisera against calbindin and parvalbumin. The excellent technical assistance of Ms E. Bor6k, Ms I. Weisz, Ms A. Z61di and Mr G. Terstyfinszki is also acknowledged. This work was supported by the Human Frontier Science Program Organization, the Howard Hughes Medical Institute and OTKA (T5532 and T16962) Hungary. Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, Budapest, H-1450, Hungary First Department of Anatomy, Semmelweis University, Medical School, Budapest, H-1450, Hungary Cited By :217 Export Date: 24 May 2020 CODEN: NRSCD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, H-1450 Budapest, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; vasoactive intestinal polypeptide, 37221-79-7 Funding details: Human Frontier Science Program Funding details: Howard Hughes Medical Institute Funding details: Hungarian Scientific Research Fund, T16962, T5532 Funding text 1: Acknowledgements We are grateful to Dr P. Somogyi for antisera against GABA and to Dr K. G. Baimbridge for antisera against calbindin and parvalbumin. The excellent technical assistance of Ms E. Bor6k, Ms I. Weisz, Ms A. Z61di and Mr G. Terstyfinszki is also acknowledged. This work was supported by the Human Frontier Science Program Organization, the Howard Hughes Medical Institute and OTKA (T5532 and T16962) Hungary. Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, Budapest, H-1450, Hungary First Department of Anatomy, Semmelweis University, Medical School, Budapest, H-1450, Hungary Cited By :228 Export Date: 23 March 2021 CODEN: NRSCD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, P.O.B. 67, H-1450 Budapest, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; vasoactive intestinal polypeptide, 37221-79-7 Funding details: Howard Hughes Medical Institute Funding details: Human Frontier Science Program Funding details: Hungarian Scientific Research Fund, T16962, T5532 Funding text 1: Acknowledgements We are grateful to Dr P. Somogyi for antisera against GABA and to Dr K. G. Baimbridge for antisera against calbindin and parvalbumin. The excellent technical assistance of Ms E. Bor6k, Ms I. Weisz, Ms A. Z61di and Mr G. Terstyfinszki is also acknowledged. This work was supported by the Human Frontier Science Program Organization, the Howard Hughes Medical Institute and OTKA (T5532 and T16962) Hungary. Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, Budapest, H-1450, Hungary First Department of Anatomy, Semmelweis University, Medical School, Budapest, H-1450, Hungary Cited By :228 Export Date: 24 March 2021 CODEN: NRSCD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, P.O.B. 67, H-1450 Budapest, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; vasoactive intestinal polypeptide, 37221-79-7 Funding details: Howard Hughes Medical Institute Funding details: Human Frontier Science Program Funding details: Hungarian Scientific Research Fund, T16962, T5532 Funding text 1: Acknowledgements We are grateful to Dr P. Somogyi for antisera against GABA and to Dr K. G. Baimbridge for antisera against calbindin and parvalbumin. The excellent technical assistance of Ms E. Bor6k, Ms I. Weisz, Ms A. Z61di and Mr G. Terstyfinszki is also acknowledged. This work was supported by the Human Frontier Science Program Organization, the Howard Hughes Medical Institute and OTKA (T5532 and T16962) Hungary. Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, Budapest, H-1450, Hungary First Department of Anatomy, Semmelweis University, Medical School, Budapest, H-1450, Hungary Cited By :229 Export Date: 6 April 2021 CODEN: NRSCD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, P.O.B. 67, H-1450 Budapest, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; vasoactive intestinal polypeptide, 37221-79-7 Funding details: Howard Hughes Medical Institute Funding details: Human Frontier Science Program Funding details: Hungarian Scientific Research Fund, T16962, T5532 Funding text 1: Acknowledgements We are grateful to Dr P. Somogyi for antisera against GABA and to Dr K. G. Baimbridge for antisera against calbindin and parvalbumin. The excellent technical assistance of Ms E. Bor6k, Ms I. Weisz, Ms A. Z61di and Mr G. Terstyfinszki is also acknowledged. This work was supported by the Human Frontier Science Program Organization, the Howard Hughes Medical Institute and OTKA (T5532 and T16962) Hungary. Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O.B. 67, Budapest, H-1450, Hungary First Department of Anatomy, Semmelweis University, Medical School, Budapest, H-1450, Hungary Cited By :229 Export Date: 14 April 2021 CODEN: NRSCD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, P.O.B. 67, H-1450 Budapest, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; vasoactive intestinal polypeptide, 37221-79-7 Funding details: Howard Hughes Medical Institute Funding details: Human Frontier Science Program Funding details: Hungarian Scientific Research Fund, T16962, T5532 Funding text 1: Acknowledgements We are grateful to Dr P. Somogyi for antisera against GABA and to Dr K. G. Baimbridge for antisera against calbindin and parvalbumin. The excellent technical assistance of Ms E. Bor6k, Ms I. Weisz, Ms A. Z61di and Mr G. Terstyfinszki is also acknowledged. This work was supported by the Human Frontier Science Program Organization, the Howard Hughes Medical Institute and OTKA (T5532 and T16962) Hungary. LA - English DB - MTMT ER - TY - JOUR AU - Freund, Tamás AU - Buzsáki, György TI - Interneurons of the hippocampus JF - HIPPOCAMPUS J2 - HIPPOCAMPUS VL - 6 PY - 1996 SP - 347 EP - 470 PG - 124 SN - 1050-9631 DO - 10.1002/(SICI)1098-1063(1996)6:4<347::AID-HIPO1>3.0.CO;2-I UR - https://m2.mtmt.hu/api/publication/107931 ID - 107931 N1 - Funding Agency and Grant Number: NINDS NIH HHS [NS34994] Funding Source: Medline LA - English DB - MTMT ER - TY - JOUR AU - Gulyás, Attila AU - Hájos, Norbert AU - Freund, Tamás TI - Interneurons containing calretinin are specialized to control other interneurons in the rat hippocampus JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 16 PY - 1996 IS - 10 SP - 3397 EP - 3411 PG - 15 SN - 0270-6474 DO - 10.1523/jneurosci.16-10-03397.1996 UR - https://m2.mtmt.hu/api/publication/107722 ID - 107722 N1 - Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest H-1450, Hungary Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O. Box 67, Budapest H-1450, Hungary Cited By :425 Export Date: 17 May 2023 CODEN: JNRSD Correspondence Address: Freund, T.F.; Institute of Experimental Medicine, P.O. Box 67, Budapest H-1450, Hungary Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; cholecystokinin, 9011-97-6, 93443-27-7; vasoactive intestinal polypeptide, 37221-79-7 LA - English DB - MTMT ER - TY - JOUR AU - Hájos, Norbert AU - Acsády, László AU - Freund, Tamás TI - Target selectivity and neurochemical characteristics of VIP-immunoreactive interneurons in the rat dentate gyrus JF - EUROPEAN JOURNAL OF NEUROSCIENCE J2 - EUR J NEUROSCI VL - 8 PY - 1996 IS - 7 SP - 1415 EP - 1431 PG - 17 SN - 0953-816X DO - 10.1111/j.1460-9568.1996.tb01604.x UR - https://m2.mtmt.hu/api/publication/107951 ID - 107951 N1 - Institute of Experimental Medicine, Hungarian Academy of Science, POB 67, Budapest H-1450, Hungary Department of Neurology, Kuopio, SF-70211, Finland Cited By :80 Export Date: 3 August 2023 CODEN: EJONE Correspondence Address: Hajos, N.; Institute of Experimental Medicine, POB 67, Budapest, H-1450, Hungary Chemicals/CAS: Vasoactive Intestinal Peptide, 37221-79-7 LA - English DB - MTMT ER - TY - JOUR AU - Halasy, Katalin AU - Buhl, EH AU - Lőrinczi, Zoltán AU - Tamás, Gábor AU - Somogyi, Péter Pál TI - Synaptic target selectivity and input of GABAergic basket and bistratified interneurons in the CA1 area of the rat hippocampus JF - HIPPOCAMPUS J2 - HIPPOCAMPUS VL - 6 PY - 1996 IS - 3 SP - 306 EP - 329 PG - 24 SN - 1050-9631 DO - 10.1002/(SICI)1098-1063(1996)6:3<306::AID-HIPO8>3.0.CO;2-K UR - https://m2.mtmt.hu/api/publication/1446619 ID - 1446619 N1 - Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :115 Export Date: 21 August 2019 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :115 Export Date: 23 August 2019 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :118 Export Date: 24 May 2020 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :119 Export Date: 30 December 2020 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :119 Export Date: 23 March 2021 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :119 Export Date: 24 March 2021 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :120 Export Date: 6 April 2021 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Oxford, United Kingdom Department of Zoology and Cell Biology, József Attila University, Szeged, Hungary Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, Romania MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Cited By :120 Export Date: 7 April 2021 CODEN: HIPPE Correspondence Address: Buhl, E.H.; MRC Anatomical Neuropharmacol. Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; biocytin, 576-19-2 AB - To assess the position of interneurons in the hippocampal network, fast spiking cells were recorded intracellularly in vitro and filled with biocytin. Sixteen non-principal cells were selected on the basis of 1) cell bodies located in the pyramidal layer and in the middle of the slice, 2) extensive labeling of their axons, and 3) a branching pattern of the axon indicating that they were not axo-axonic cells. Examination of their efferent synapses (n = 400) demonstrated that the cells made synapses on cell bodies, dendritic shafts, spines, and axon initial segments (AIS), Statistical analysis of the distribution of different postsynaptic elements, together with published data (n = 288) for 12 similar cells, showed that the interneurons were heterogeneous with regard to the frequency of synapses given to different parts of pyramidal cells. When the cells were grouped according to whether they had less or more than 40% somatic synaptic targets, each population appeared homogeneous. The population (n = 19) innervating a high proportion of somata (53 +/- 10%, SD) corresponds to basket cells. They also form synapses with proximal dendrites (44 +/- 12%) and rarely with AISs and spines. One well-filled basket cell had 8,859 boutons within the slice, covering an area of 0.331 mm(2) of pyramidal layer tangentially and containing 7,150 pyramidal cells, 933 (13%) of which were calculated to be innervated, assuming that each pyramidal cell received nine to ten synapses. It was extrapolated that the intact axon probably had about 10,800 boutons innervating 1,140 pyramids. The proportion of innervated pyramidal cells decreased from 28% in the middle to 4% at the edge of the axonal field. The other group of neurons, the bistratified cells (n = 9), showed a preference for dendritic shafts (79 +/- 8%) and spines (17 +/- 8%) as synaptic targets, rarely terminating on somata (4 +/- 8%). Their axonal field was significantly larger (1,250 +/- 180 mu m) in the medio-lateral direction than that of basket cells (760 +/- 130 mu m). The axon terminals of bistratified cells were smaller than those of basket cells. Furthermore, in contrast to bistratified cells, basket cells had a significant proportion of dendrites in stratum lacunosum-moleculare suggesting a direct entorhinal input. The results define two distinct types of GABAergic neuron innervating pyramidal cells in a spatially segregated manner and predict different functions for the two inputs. The perisomatic termination of basket cells is suited for the synchronization of a subset of pyramidal cells that they select from the population within their axonal field, whereas the termination of bistratified cells in conjunction with Schaffer collateral/commissural terminals may govern the timing of CA3 input and/or voltage-dependent conductances in the dendrites. (C) 1996 Wiley-Liss, Inc. LA - English DB - MTMT ER - TY - JOUR AU - Miles, R AU - Tóth, Katalin AU - Gulyás, Attila AU - Hájos, Norbert AU - Freund, Tamás TI - Differences between somatic and dendritic inhibition in the hippocampus JF - NEURON J2 - NEURON VL - 16 PY - 1996 IS - 4 SP - 815 EP - 823 PG - 9 SN - 0896-6273 DO - 10.1016/S0896-6273(00)80101-4 UR - https://m2.mtmt.hu/api/publication/107721 ID - 107721 N1 - Megjegyzés-25002645 Megjegyzés-21894988 Z9: 477 Lab. de Neurobiologie Cellulaire, Institut Pasteur, 25 rue de Dr. Roux, 75724 Paris, France Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1450 Budapest, Hungary Cited By :713 Export Date: 24 March 2021 CODEN: NERNE Correspondence Address: Miles, R.; Lab. de Neurobiologie Cellulaire, 25 rue de Dr. Roux, 75724 Paris, France Chemicals/CAS: biocytin, 576-19-2 Funding details: European Science Foundation, ESF Funding details: Human Frontier Science Program, HFSP Funding details: Hungarian Scientific Research Fund, OTKA, T5532 Funding text 1: Correspondence should be addressed to R. M. This work was supported by the Human Science Frontier Program, the European Science Foundation, the Ministére de la Recherche et de la Technologie, and OTKA (T5532) Hungary. We thank B. Barbour for helpful comments and E. Borók, I. Weisz, and G. Terstyánszky for excellent technical assistance. Lab. de Neurobiologie Cellulaire, Institut Pasteur, 25 rue de Dr. Roux, 75724 Paris, France Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1450 Budapest, Hungary Cited By :714 Export Date: 6 April 2021 CODEN: NERNE Correspondence Address: Miles, R.; Lab. de Neurobiologie Cellulaire, 25 rue de Dr. Roux, 75724 Paris, France Chemicals/CAS: biocytin, 576-19-2 Funding details: European Science Foundation, ESF Funding details: Human Frontier Science Program, HFSP Funding details: Hungarian Scientific Research Fund, OTKA, T5532 Funding text 1: Correspondence should be addressed to R. M. This work was supported by the Human Science Frontier Program, the European Science Foundation, the Ministére de la Recherche et de la Technologie, and OTKA (T5532) Hungary. We thank B. Barbour for helpful comments and E. Borók, I. Weisz, and G. Terstyánszky for excellent technical assistance. LA - English DB - MTMT ER - TY - JOUR AU - Nusser, Zoltán AU - Sieghart, W AU - Benke, D AU - Fritschy, JM AU - Somogyi, Péter Pál TI - Differential synaptic localization of two major γ-aminobutyric acid type A receptor α subunits on hippocampal pyramidal cells JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 93 PY - 1996 SP - 11939 EP - 11944 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.93.21.11939 UR - https://m2.mtmt.hu/api/publication/108775 ID - 108775 N1 - Medical Research Council, Anatomical Neuropharmacology Unit, University of Oxford, Mansfield Road, Oxford OX1 3TH, United Kingdom Department of Biochemical Psychiatry, Psychiat. Universitätsklinik, Währinger Gürtel 18-20, A-1090 Vienna, Austria Institute of Pharmacology, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland Cited By :343 Export Date: 1 August 2023 CODEN: PNASA Correspondence Address: Nusser, Z.; Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: Immune Sera; Macromolecular Substances; Receptors, GABA-A LA - English DB - MTMT ER - TY - JOUR AU - Somogyi, Péter Pál AU - Fritschy, JM AU - Benke, D AU - Roberts, JD AU - Sieghart, W TI - The gamma 2 subunit of the GABAA receptor is concentrated in synaptic junctions containing the alpha 1 and beta 2/3 subunits in hippocampus, cerebellum and globus pallidus. JF - NEUROPHARMACOLOGY J2 - NEUROPHARMACOLOGY VL - 35 PY - 1996 IS - 9-10 SP - 1425 EP - 1444 PG - 20 SN - 0028-3908 DO - 10.1016/S0028-3908(96)00086-X UR - https://m2.mtmt.hu/api/publication/2138101 ID - 2138101 N1 - Megjegyzés-22903049 Megjegyzés-21892788 Z9: 111 Megjegyzés-21894962 Z9: 111 MRC Anat. Neuropharmacology Unit, Department of Pharmacology, Mansfield Rd, Oxford OX1 3TH, United Kingdom Institute of Pharmacology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland Department of Biochemical Psychiatry, University Clinic for Psychiatry, Währinger Gurtel 18-20, A-1090 Vienna, Austria Cited By :144 Export Date: 22 May 2020 CODEN: NEPHB Correspondence Address: Somogyi, P.; MRC Anatomical Neuropharmacol. Unit, Department of Pharmacology, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: Receptors, GABA-A MRC Anat. Neuropharmacology Unit, Department of Pharmacology, Mansfield Rd, Oxford OX1 3TH, United Kingdom Institute of Pharmacology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland Department of Biochemical Psychiatry, University Clinic for Psychiatry, Währinger Gurtel 18-20, A-1090 Vienna, Austria Cited By :145 Export Date: 30 December 2020 CODEN: NEPHB Correspondence Address: Somogyi, P.; MRC Anatomical Neuropharmacol. Unit, Department of Pharmacology, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: Receptors, GABA-A MRC Anat. Neuropharmacology Unit, Department of Pharmacology, Mansfield Rd, Oxford OX1 3TH, United Kingdom Institute of Pharmacology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland Department of Biochemical Psychiatry, University Clinic for Psychiatry, Währinger Gurtel 18-20, A-1090 Vienna, Austria Cited By :145 Export Date: 1 January 2021 CODEN: NEPHB Correspondence Address: Somogyi, P.; MRC Anatomical Neuropharmacol. Unit, Department of Pharmacology, Mansfield Road, Oxford OX1 3TH, United Kingdom Chemicals/CAS: Receptors, GABA-A AB - The gamma 2 subunit is necessary for the expression of the full benzodiazepine pharmacology of GABAA receptors and is one of the major subunits in the brain. In order to determine the location of channels containing the gamma 2 subunit in relation to GABA-releasing terminals on the surface of neurons, a new polyclonal antipeptide antiserum was developed to the gamma 2 subunit and used in high resolution, postembedding, immunoelectron-microscopic procedures. Dual immunogold labelling of the same section for two subunits, and up to three sections of the same synapse reacted for different subunits, were used to characterize the subunit composition of synaptic receptors. The gamma 2 subunit was present in type 2, "symmetrical" synapses in each of the brain areas studied, with the exception of the granule cell layer of the cerebellum. The gamma 2 subunit was frequently co-localized in the same synaptic junction with the alpha 1 and beta 2/3 subunits. The immunolabelling of synapses was coincident with the junctional membrane specialization of the active zone. Immunolabelling for the receptor often occurred in multiple clusters in the synapses. In the hippocampus, the gamma 2 subunit was present in basket cell synapses on the somata and proximal dendrites and in axo-axonic cell synapses on the axon initial segment of pyramidal and granule cells. Some synapses on the dendrites of GABAergic interneurones were densely labelled for the gamma 2, alpha 1 and beta 2/3 subunits. In the cerebellum, the gamma 2 subunit was present in both distal and proximal Purkinje cell dendritic synapses established by stellate and basket cell, respectively. On the soma of Purkinje cells, basket cell synapses were only weakly labelled. Synapses on interneuron dendrites were more densely labelled for the gamma 2, alpha 1 and beta 2/3 subunits than synapses on Purkinje or granule cells. Although immunoperoxidase and immunofluorescence methods show an abundance of the gamma 2 subunit in granule cells, the labelling of Golgi synapses was much weaker with the immunogold method than that of the other cell types. In the globus pallidus, many type 2 synapses were labelled for the gamma 2 subunit together with alpha 1 and beta 2/3 subunits. The results show that gamma 2 and beta 2/3 subunits receptor channels are highly concentrated in GABAergic synapses that also contain the alpha 1 and beta 2/3 subunits. Channels containing the gamma 2 subunit are expressed in synapses on functionally distinct domains of the same neuron receiving GABA from different presynaptic sources. There are quantitative differences in the density of GABAA receptors at synapses on different cell types in the same brain area. LA - English DB - MTMT ER - TY - JOUR AU - Blasco-Ibanez, Jose Miguel AU - Freund, Tamás TI - Synaptic input of horizontal interneurons in striatum oriens of the hippocampal CA1 subfield: Structural basis of feed-back activation JF - EUROPEAN JOURNAL OF NEUROSCIENCE J2 - EUR J NEUROSCI VL - 7 PY - 1995 SP - 2170 EP - 2180 PG - 11 SN - 0953-816X DO - 10.1111/j.1460-9568.1995.tb00638.x UR - https://m2.mtmt.hu/api/publication/107793 ID - 107793 N1 - Cited By :175 Export Date: 3 August 2023 Correspondence Address: Freund, T.F. Chemicals/CAS: Receptors, Metabotropic Glutamate LA - English DB - MTMT ER - TY - JOUR AU - BRAGIN, A AU - Jandó, Gábor AU - Nádasdy, Zoltán AU - HETKE, J AU - WISE, K AU - Buzsáki, György TI - GAMMA (40-100-HZ) OSCILLATION IN THE HIPPOCAMPUS OF THE BEHAVING RAT JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 15 PY - 1995 IS - 1 SP - 47 EP - 60 PG - 14 SN - 0270-6474 DO - 10.1523/jneurosci.15-01-00047.1995 UR - https://m2.mtmt.hu/api/publication/1428428 ID - 1428428 AB - The cellular generation and spatial distribution of gamma frequency (40-100 Hz) activity was examined in the hippocampus of the awake rat. Field potentials and unit activity were recorded by multiple site silicon probes (5- and 16-site shanks) and wire electrode arrays. Gamma waves were highly coherent along the long axis of the dentate hilus, but average coherence decreased rapidly in the CA3 and CA1 directions. Analysis of short epochs revealed large fluctuations in coherence values between the dentate and CA1 gamma waves, Current source density analysis revealed large sinks and sources in the dentate gyrus with spatial distribution similar to the dipoles evoked by stimulation of the perforant path, The frequency changes of gamma and theta waves positively correlated (40-100 Hz and 5-10 Hz, respectively), Putative interneurons in the dentate gyrus discharged at gamma frequency and were phase-locked to the ascending part of the gamma waves recorded from the hilus, Following bilateral lesion of the entorhinal cortex the power and frequency of hilar gamma activity significantly decreased or disappeared. Instead, a large amplitude but slower gamma pattern (25-50 Hz) emerged in the CA3-CA1 network, We suggest that gamma oscillation emerges from an interaction between intrinsic oscillatory properties of interneurons and the network properties of the dentate gyrus. We also hypothesize that under physiological conditions the hilar gamma oscillation may be entrained by the entorhinal rhythm and that gamma oscillation in the CA3-CA1 circuitry is suppressed by either the hilar region or the entorhinal cortex. LA - English DB - MTMT ER - TY - JOUR AU - COBB, SR AU - BUHL, EH AU - Halasy, Katalin AU - PAULSEN, O AU - Somogyi, Péter Pál TI - SYNCHRONIZATION OF NEURONAL-ACTIVITY IN HIPPOCAMPUS BY INDIVIDUAL GABAERGIC INTERNEURONS JF - NATURE J2 - NATURE VL - 378 PY - 1995 IS - 6552 SP - 75 EP - 78 PG - 4 SN - 0028-0836 DO - 10.1038/378075a0 UR - https://m2.mtmt.hu/api/publication/1661000 ID - 1661000 N1 - MRC Anatomical Neuropharmacology Unit, University Department of Pharmacology, Mansfield Road, Oxford, OX1 3TH, United Kingdom Department of Zoology & Cell Biology, Jozsef Attila University, Szeged, H-6722, Hungary Cited By :1114 Export Date: 6 April 2021 Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; gamma-Aminobutyric Acid, 56-12-2; Receptors, GABA-A AB - SYNCHRONIZATION Of neuronal activity is fundamental in the operation of cortical networks(1). With respect to an ongoing synchronized oscillation, the precise timing of action potentials is an attractive candidate mechanism for information coding(2-5) Networks of inhibitory interneurons have been proposed to have a role in entraining cortical, synchronized 40-Hz activity(6,7). Here we demonstrate that individual GABAergic interneurons(8) can effectively phase spontaneous firing and subthreshold oscillations in hippocampal pyramidal cells at theta frequencies (4-7 Hz). The efficiency of this entrainment is due to interaction of GABA(A)-receptor-mediated hyperpolarizing synaptic events with intrinsic oscillatory mechanisms tuned to this frequency range in pyramidal cells. Moreover, this GABAergic mechanism is sufficient to synchronize the firing of pyramidal cells. Thus, owing to the divergence of each GABAergic interneuron(9,10), more than a thousand pyramidal cells may share a common temporal reference established by an individual interneuron. LA - English DB - MTMT ER - TY - JOUR AU - Nusser, Zoltán AU - Roberts, JDB AU - Baude, A AU - Richards, JG AU - Sieghart, W AU - Somogyi, Péter Pál TI - Immunocytochemical localization of the α1 and β2/3 subunits of the GABA~A receptor in relation to specific GABAergic synapses in the dentate gyrus JF - EUROPEAN JOURNAL OF NEUROSCIENCE J2 - EUR J NEUROSCI VL - 7 PY - 1995 SP - 630 EP - 646 PG - 17 SN - 0953-816X DO - 10.1111/j.1460-9568.1995.tb00667.x UR - https://m2.mtmt.hu/api/publication/108528 ID - 108528 N1 - Medical Research Council, Anatomical Neuropharmacology Unit, University of Oxford, Mansfield Road, Oxford, OX1 3TH, United Kingdom Cnrs Laboratoire de Neurobiologie, 31 chemin Joseph Aiguier, Marseille, 13402, France Pharma Division, Preclinical Research, F. Hoffman-La Roche Ltd, Basel, CH-4002, Switzerland Department of Biochemical Psychiatry, Psychiartrische Universitätsklinik, Währinger Gürtel 18-20, Vienna, A-1090, Austria Cited By :137 Export Date: 6 April 2021 Correspondence Address: Nusser, Z.; Medical Research Council, Mansfield Road, Oxford, OX1 3TH, United Kingdom Chemicals/CAS: gamma-Aminobutyric Acid, 56-12-2; Receptors, GABA-A LA - English DB - MTMT ER - TY - JOUR AU - Sík, Attila AU - Penttonen, M AU - Ylinen, A AU - Buzsaki, G TI - Hippocampal CA1 interneurons: an in vivo intracellular labeling study. JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 15 PY - 1995 IS - 10 SP - 6651 EP - 6665 PG - 15 SN - 0270-6474 DO - 10.1523/jneurosci.15-10-06651.1995 UR - https://m2.mtmt.hu/api/publication/3301452 ID - 3301452 N1 - Ctr. for Molec. and Behav. Neurosci., Rutgers, State Univ. of New Jersey, Newark, NJ 07102, United States Ctr. for Molec. and Behav. Neurosci., Rutgers University, 197 University Avenue, Newark, NJ 07102, United States Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Department of Psychology, University of Jyväskylä, Jyväskylä, Finland Department of Neurology, University of Kuopio, Kuopio, Finland Cited By :524 Export Date: 24 May 2024 CODEN: JNRSD Correspondence Address: Buzsaki, G.; Molecular/Behavioral Neurosci. Ctr., 197 University Avenue, Newark, NJ 07102, United States AB - Fast spiking interneurons in the CA1 area of the dorsal hippocampus were recorded from and filled with biocytin in anesthetized rats. The full extent of their dendrites and axonal arborizations as well as their calcium binding protein content were examined. Based on the spatial extent of axon collaterals, local circuit cells (basket and O-LM neurons) and long-range cells (bistratified, trilaminar, and backprojection neurons) could be distinguished. Basket cells were immunoreactive for parvalbumin and their axon collaterals were confined to the pyramidal layer. A single basket cell contacted more than 1500 pyramidal neurons and 60 other parvalbumin-positive interneurons. Commissural stimulation directly discharged basket cells, followed by an early and late IPSPs, indicating interneuronal inhibition of basket cells. The dendrites of another local circuit neuron (O-LM) were confined to stratum oriens and it had a small but high-density axonal terminal field in stratum lacunosum-moleculare. The fastest firing cell of all interneurons was a calbindin-immunoreactive bistratified neuron with axonal targets in stratum oriens and radiatum. Two neurons with their cell bodies in the alveus innervated the CA3 region (backprojection cells), in addition to rich axon collaterals in the CA1 region. The trilaminar interneuron had axon collaterals in strata radiatum, oriens and pyramidale with its dendrites confined to stratum oriens. Commissural stimulation evoked an early EPSP-IPSP-late depolarizing potential sequence in this cell. All interneurons formed symmetric synapses with their targets at the electron microscopic level. These findings indicate that interneurons with distinct axonal targets have differential functions in shaping the physiological patterns of the CA1 network. LA - English DB - MTMT ER - TY - JOUR AU - Soltesz, Ivan AU - MODY, I TI - CA2+-DEPENDENT PLASTICITY OF MINIATURE INHIBITORY POSTSYNAPTIC CURRENTS AFTER AMPUTATION OF DENDRITES IN CENTRAL NEURONS JF - JOURNAL OF NEUROPHYSIOLOGY J2 - J NEUROPHYSIOL VL - 73 PY - 1995 IS - 5 SP - 1763 EP - 1773 PG - 11 SN - 0022-3077 UR - https://m2.mtmt.hu/api/publication/2941572 ID - 2941572 AB - 1. The effects of cutting off the bulk (>2/3) of the dendritic tree (dendrotomy) on GABAergic miniature inhibitory postsynaptic currents (mIPCSs) were studied in granule cells of the adult rat dentate gyrus in 400-mu m-thick slices in vitro. 2. After dendrotomy carried out in warm (32 degrees C) control artificial cerebrospinal fluid (ACSF), only small antidromic population spikes could be evoked in the granule cell layer, and no viable whole cell recordings could be obtained. However, when dendrotomy was performed in cold (8-10 degrees C) control ACSF, the amplitude of the antidromic population spikes increased, and stable whole cell recordings became possible. 3. Whole cell recordings, with CsCl-filled pipettes, from granule cells dendrotomized in cold control ACSF, revealed significant alterations, lasting >10 h, in the decay kinetics of mIPSCs. The change consisted of a calcium-dependent transformation of the normal, single exponential decay into a prolonged double exponential that effectively increased the charge transferred by the synaptic events (the total area of the currents) by 67%. When 30 mM 1,2 bis-(2-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA) was included in the pipette, the changes in the mIPSCs decay kinetics could still be observed after dendrotomy, indicating that the maintenance phase of this plasticity did not depend on elevated intracellular calcium levels. 4. Viable whole cell recordings could also be obtained in dendrotomized granule cells when the amputation of dendrites was carried out at 32 degrees C after incubation for 2 h with the cell-permeant Ca2+ chelator, BAPTA-AM (50 mu M), or the cutting process was done in an ACSF containing either a combination of excitatory amino acid receptor antagonists 2-amino-5-phosphonovaleric acid (APV; 25 mu M) + 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 10 mu M), a blocker of intracellular Ca2+ release dantrolene-Na (20 mu M), or the voltage-gated Na+ channel blocker tetrodotoxin (TTX; 1 mu M). 5. After dendrotomy in BAPTA-AM, APV + CNQX, APV + CNQX + TTX, and/or dantrolene, the changes in decay kinetics were prevented, indicating that a rise in intracellular Ca2+ concentration plays a pivotal role in this plasticity. 6. Computer simulations of mIPSCs suggested that changes in single channel kinetics alone can, in principle, account for the Ca2+-dependent changes in mIPSC decay kinetics. 7. These findings are consistent with a lasting Ca2+-dependent increase in gamma-aminobutyric acid-A (GABAA) receptor function in cells that survive physical injury to their dendrites. LA - English DB - MTMT ER - TY - JOUR AU - BUHL, EH AU - Halasy, Katalin AU - Somogyi, Péter Pál TI - DIVERSE SOURCES OF HIPPOCAMPAL UNITARY INHIBITORY POSTSYNAPTIC POTENTIALS AND THE NUMBER OF SYNAPTIC RELEASE SITES JF - NATURE J2 - NATURE VL - 368 PY - 1994 IS - 6474 SP - 823 EP - 828 PG - 6 SN - 0028-0836 DO - 10.1038/368823a0 UR - https://m2.mtmt.hu/api/publication/1660995 ID - 1660995 N1 - Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Mansfield Road, Oxford 0X1 3TH, United Kingdom Department of Zoology, Jozsef Attila University, Szeged, Hungary Cited By :569 Export Date: 6 April 2021 Correspondence Address: Buhl, E.H.; Medical Research Council, Mansfield Road, Oxford 0X1 3TH, United Kingdom Chemicals/CAS: Receptors, GABA-A AB - Dual intracellular recordings from microscopically identified neurons in the hippocampus reveal that the synaptic terminals of three morphologically distinct types of interneuron act through GABA(A) receptors. Each type of interneuron forms up to 12 synaptic contacts with a postsynaptic principal neuron, but each interneuron innervates a different domain of the surface of the postsynaptic neuron. Different kinetics of the postsynaptic effects, together with the strategic placement of synapses, indicate that these GABAergic interneurons serve distinct functions in the cortical network. LA - English DB - MTMT ER - TY - JOUR AU - Sík, Attila AU - Ylinen, A AU - Penttonen, M AU - Buzsaki, G TI - Inhibitory CA1-CA3-hilar region feedback in the hippocampus. JF - SCIENCE J2 - SCIENCE VL - 265 PY - 1994 IS - 5179 SP - 1722 EP - 1724 PG - 3 SN - 0036-8075 DO - 10.1126/science.8085161 UR - https://m2.mtmt.hu/api/publication/3301454 ID - 3301454 N1 - Export Date: 27 January 2024; CODEN: SCIEA AB - The organization of the hippocampus is generally thought of as a series of cell groups that form a unidirectionally excited chain, regulated by localized inhibitory circuits. With the use of in vivo intracellular labeling, histochemical, and extracellular tracing methods, a longitudinally widespread, inhibitory feedback in rat brain from the CA1 area to the CA3 and hilar regions was observed. This long-range, cross-regional inhibition may allow precise synchronization of population activity by timing the occurrence of action potentials in the principal cells and may contribute to the coordinated induction of synaptic plasticity in distributed networks. LA - English DB - MTMT ER - TY - JOUR AU - Soltesz, Ivan AU - MODY, I TI - PATCH-CLAMP RECORDINGS REVEAL POWERFUL GABAERGIC INHIBITION IN DENTATE HILAR NEURONS JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 14 PY - 1994 IS - 4 SP - 2365 EP - 2376 PG - 12 SN - 0270-6474 DO - 10.1523/jneurosci.14-04-02365.1994 UR - https://m2.mtmt.hu/api/publication/2941581 ID - 2941581 AB - Whole-cell and cell-attached patch-clamp recordings were used to investigate the nature of GABA(A) receptor-mediated inhibition in the adult rat dentate gyrus in standard 400-mu m-thick horizontal slices. In the presence of the glutamate receptor antagonists D-2-amino-5-phosphonovaleric acid and 6-cyano-7-nitroquinoxaline-2,3-dione, whole-cell voltage-clamp experiments with chloride-filled electrodes ([Cl-](in) = [Cl-](out)) revealed a high degree of spontaneous activity(10-60 Hz) in all hilar neurons (HNs) recorded with access resistances lower than 20 M Omega. The events were inward at negative holding potentials, reversed at around the Cl- equilibrium potential, and were completely abolished by the specific antagonists of the GABA(A) receptor channel picrotoxin and SR-95531 in a reversible manner, indicating that they were spontaneous inhibitory postsynaptic currents (sIPSCs) mediated by GABA(A) receptors. The majority of the slPSCs were TTX-insensitive miniature currents resulting from the action potential-independent release of GABA. The 10-90% rise times and the monoexponential decay time constants of the sIPSCs were significantly longer in HNs than those found in neighboring granule cells (GCs). Furthermore, the decay time constant of the hilar slPSCs was not voltage dependent, contrary to the voltage dependency of the decay time constant of the sIPSCs recorded from GCs. As HNs have longer electrotonic length than GCs do, dendritic filtering may contribute to the kinetic differences. Nonstationary fluctuation analysis showed that whereas the number of channels open at the peak of individual sIPSCs was similar, the single-channel conductances significantly differed between the two cell groups. The 21% smaller single-channel conductance and the existence of electrotonically close GABAergic synapses on HNs indicate that dendritic filtering alone cannot explain the differences between HNs and GCs. The distinct subunit composition of the GABA(A) receptor channels in HNs and GCs may also be responsible for the altered kinetics of IPSCs in HNs. However, the subunit specific benzodiazepine agonist zolpidem (3 mu M) prolonged the monoexponential decay time constants in both HNs and GCs. Thus, differences between the GABA(A) receptors of the two cell types are not due to a simple all-or-none presence/absence of the alpha 5 subunit. In order to determine the effect of the activation of GABA(A) receptors on the resting membrane potential in HNs and GCs in a nonintrusive way, we used single potassium channels as transmembrane voltage sensors by measuring the change in their conductance in cell-attached recordings in response to the GABA(A) agonist muscimol. GABA(A) receptor activation resulted in a strong peak depolarization (about 16 mV) in GCs but induced only small (about 4 mV) depolarizations in HNs. These results reveal for the first time that spontaneous activation of GABA(A) receptors takes place in HNs with a high frequency. Thus, while significant differences exist in the way GABAergic inhibition operates in the two neighboring neuronal population, it is highly unlikely that a general lack of inhibition can explain the extreme vulnerability of HNs to excitotoxic insults. LA - English DB - MTMT ER - TY - JOUR AU - Gulyás, Attila AU - Miles, R AU - Hájos, Norbert AU - Freund, Tamás TI - Precision and variability in postsynaptic target selection of inhibitory cells in the hippocampal CA3 region JF - EUROPEAN JOURNAL OF NEUROSCIENCE J2 - EUR J NEUROSCI VL - 5 PY - 1993 IS - 12 SP - 1729 EP - 1751 PG - 23 SN - 0953-816X DO - 10.1111/j.1460-9568.1993.tb00240.x UR - https://m2.mtmt.hu/api/publication/107599 ID - 107599 N1 - Institute of Experimental Medicine, Hungarian Academy of Sciences, PO Box 67, Budapest, H-1450, Hungary Laboratoire de Neurobiologie Cellulaire, Institut Pasteur, INSERM U261, 25 Rue de Dr Roux, Paris, 75264, France Cited By :180 Export Date: 6 April 2021 Correspondence Address: Freund, T.F. Chemicals/CAS: biocytin, 576-19-2; Lysine, 56-87-1 Institute of Experimental Medicine, Hungarian Academy of Sciences, PO Box 67, Budapest, H-1450, Hungary Laboratoire de Neurobiologie Cellulaire, Institut Pasteur, INSERM U261, 25 Rue de Dr Roux, Paris, 75264, France Cited By :180 Export Date: 7 April 2021 Correspondence Address: Freund, T.F. Chemicals/CAS: biocytin, 576-19-2; Lysine, 56-87-1 LA - English DB - MTMT ER - TY - JOUR AU - Han, ZS AU - Buhl, EH AU - Lőrinczi, Zoltán AU - Somogyi, Péter Pál TI - A high degree of spatial selectivity in the axonal and dendritic domains of physiologically identified local-circuit neurons in the dentate gyrus of the rat hippocampus. A High Degree of Spatial Selectivity in the Axonal and Dendritic Domains of Physiologically Identified Local‐circuit Neurons in the Dentate Gyms of the Rat Hippocampus JF - EUROPEAN JOURNAL OF NEUROSCIENCE J2 - EUR J NEUROSCI VL - 5 PY - 1993 IS - 5 SP - 395 EP - 410 PG - 16 SN - 0953-816X DO - 10.1111/j.1460-9568.1993.tb00507.x UR - https://m2.mtmt.hu/api/publication/2138105 ID - 2138105 N1 - Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Mansfield Road, Oxford, OX1 3TH, United Kingdom Department of Anatomy, University of Medicine and Pharmacy, Tirgu Mures, 4300, Romania Cited By :341 Export Date: 3 August 2023 Correspondence Address: Somogyi, P. Chemicals/CAS: biocytin, 576-19-2; Lysine, 56-87-1 AB - The axonal and dendritic domains of neurons with extensive, locally arborizing axons were delineated in the dentate gyrus of the rat hippocampus. In horizontally cut slice preparations neurons were briefly recorded and subsequently filled with biocytin when one or several of the following physiological properties were observed: (i) high-amplitude short-latency spike afterhyperpolarization; (ii) lack of spike frequency adaptation; (iii) high firing rate in response to depolarizing current. In a sample of 14 neurons, sufficient dendritic and/or axonal detail was recovered to identify them as non-principal cells, i.e. non-granule, non-mossy cells. Five distinct types of cells were recognized, based on the spatial distribution of dendrites, presumably reflecting the availability of afferents, and on the basis of the highly selective distribution of their axon terminals, indicating synaptic target selectivity. They are: (1) the hilar cell forming a dense axonal plexus in the commissural and association pathway terminal field (HICAP cell; horizontal axon extent 1.6 mm) in the inner one-third of the molecular layer, and having dendrites extending from the hilus to the top of the molecular layer; (2) the hilar cell with its axon ramifying in the perforant path terminal field (HIPP cell, horizontal axon extent 2.0 mm) in the outer two-thirds of the molecular layer, whereas its spiny dendrites were restricted to the hilus; (3) the molecular layer cell with its dendritic and axonal domains confined to the perforant path terminal zone (MOPP cell, horizontal extent of axon 2.0 mm); (4) the dentate basket cell (horizontal axon extent 0.9 mm) had most of its axon concentrated in the granule cell layer, the remainder being localized in the inner molecular layer and hilus; (5) the hilar chandelier cell, or axo-axonic cell (horizontal axon extent 1.1 mm), densely innervating the granule cell layer with fascicles of radially oriented terminal rows, and also forming an extensive plexus in the hilus. The three cell types having their somata in the hilus projected to granule cells at the same septo-temporal level where their cell bodies were located. The results demonstrate that there is a spatially selective innervation of the granule cells by at least five distinct types of dentate neurons, which terminate in several instances in mutually exclusive domains. Their dendrites may have access to all (HICAP cell) or only a few (e.g. HIPP and MOPP cell) of the hippocampal afferents. This arrangement provides a framework for independent interaction between the output of local circuit neurons and subsets of excitatory afferents providing input to principal cells. LA - English DB - MTMT ER - TY - JOUR AU - Soltesz, Ivan AU - DESCHENES, M TI - LOW-FREQUENCY AND HIGH-FREQUENCY MEMBRANE-POTENTIAL OSCILLATIONS DURING THETA ACTIVITY IN CA1 AND CA3 PYRAMIDAL NEURONS OF THE RAT HIPPOCAMPUS UNDER KETAMINE-XYLAZINE ANESTHESIA JF - JOURNAL OF NEUROPHYSIOLOGY J2 - J NEUROPHYSIOL VL - 70 PY - 1993 IS - 1 SP - 97 EP - 116 PG - 20 SN - 0022-3077 DO - 10.1152/jn.1993.70.1.97 UR - https://m2.mtmt.hu/api/publication/2941583 ID - 2941583 AB - 1. Intracellularly recorded low- and high-frequency (4-6 and 25-50 Hz, respectively), rhythmic, spontaneous membrane potential oscillations were investigated in pyramidal neurons of the rat hippocampus in vivo, during theta(THETA, 4-6 Hz)electroencephalographic (EEG) activity, under ketamine-xylazine anesthesia. 2. The EEG activity showed two spectral peaks, one in the THETA range. the other at higher frequencies (25-50 Hz). On the basis of their electrophysiological and pharmacological properties, it was concluded that the EEG THETA-waves, and the fast EEG rhythm, recorded during ketamine-xylazine anesthesia, share the basic properties of those THETA and fast rhythms that are recorded under the effects of other types of anesthetics. 3. When intracellular recordings (n = 32) were made with electrodes filled with potassium-acetate (K-acetate), the only CA1 and CA3 pyramidal cells (PCs) considered for further analysis were those that did not fire rhythmically at most or each cycle of the THETA rhythm at the resting membrane potential. During EEG-THETA, the membrane potential (V(m)) of these cells showed a prominent oscillation (3-15 mV) with frequencies similar to those of the EEG-THETA (the intracellular THETA rhythm, intra-THETA). 4. The frequency of the intra-THETA was independent of the V(m). However, the phase difference between the intra-THETA and the EEG-THETA was voltage dependent in both types of cells. CA1 PCs showed a large ( 120-180-degrees, where 360-degrees is the full cycle), gradual shift in the phase difference between the intra-THETA and the EEG-THETA, when the membrane was hyperpolarized to -85 from -65 mV. Although CA3 PCs displayed a larger variability in their phase-voltage relations. a voltage-dependent phase shift (90-180-degrees) could be observed in CA3 PCs as well. 5. Although the amplitude of the intra-THETA in both CA1 and CA3 PCs could display large, sudden, spontaneous changes at a given V(m), the amplitude-V(m) plots tended to show a minimum between -70 and -80 mV. Spontaneous changes in the amplitude of the intra-THETA did not affect the phase difference between the intra- and the EEG-THETA rhythms. 6. Intracellular injection of QX-314 (50-100 mM) did not change the phase-V(m) or the amplitude-V(m) relationships of CA1 PCs. 7. Intracellular injection of chloride (Cl-) ions greatly reduced the voltage dependency of the phase difference and revealed fast (duration: 20-25 ms), depolarizing potentials (5-20 mV), which appeared at high frequencies (25-50 Hz), amplitude modulated at THETA-frequencies. 8. These findings are consistent with the hypothesis that rhythmic, high-frequency, gamma-aminobutyric acid-A (GABA(A))-receptor-mediated inhibitory postsynaptic potentials have an important role in the generation of both the THETA and the fast hippocampal rhythms. LA - English DB - MTMT ER - TY - JOUR AU - Buzsáki, György AU - Horváth, Zsolt AU - Urioste, R AU - Hetke, J AU - Wise, K TI - High-frequency network oscillation in the hippocampus JF - SCIENCE J2 - SCIENCE VL - 256 PY - 1992 IS - 5059 SP - 1025 EP - 1027 PG - 3 SN - 0036-8075 DO - 10.1126/science.1589772 UR - https://m2.mtmt.hu/api/publication/2550096 ID - 2550096 LA - English DB - MTMT ER - TY - JOUR AU - Freund, Tamás AU - Antal, Miklós TI - GABA-containing neurons in the septum control inhibitory interneurons in the hippocampus JF - NATURE J2 - NATURE VL - 336 PY - 1988 SP - 170 EP - 173 PG - 4 SN - 0028-0836 DO - 10.1038/336170a0 UR - https://m2.mtmt.hu/api/publication/107532 ID - 107532 LA - English DB - MTMT ER - TY - JOUR AU - Buzsáki, György TI - FEEDFORWARD INHIBITION IN THE HIPPOCAMPAL-FORMATION JF - PROGRESS IN NEUROBIOLOGY: AN INTERNATIONAL REVIEW JOURNAL J2 - PROG NEUROBIOL VL - 22 PY - 1984 IS - 2 SP - 131 EP - 153 PG - 23 SN - 0301-0082 DO - 10.1016/0301-0082(84)90023-6 UR - https://m2.mtmt.hu/api/publication/2587252 ID - 2587252 N1 - Export Date: 27 January 2024; CODEN: PGNBA LA - English DB - MTMT ER -