TY  - JOUR
AU  - Kardos, Julianna
AU  - Kovács, Ilona
AU  - Szárics, Éva
AU  - Kovács, Richárd
AU  - Skuban, N
AU  - Nyitrai, Gabriella
AU  - Dobolyi, Árpád
AU  - Juhász, Gábor Dénes
TI  - Uridine activates fast transmembrane Ca2+ ion fluxes in rat brain homogenates
JF  - NEUROREPORT
J2  - NEUROREPORT
VL  - 10
PY  - 1999
IS  - 7
SP  - 1577
EP  - 1582
PG  - 6
SN  - 0959-4965
DO  - 10.1097/00001756-199905140-00034
UR  - https://m2.mtmt.hu/api/publication/112598
ID  - 112598
N1  - [9/1999]
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Dobolyi, Árpád
AU  - Reichart, Anikó
AU  - Szikra, Tamás
AU  - Szilágyi, Nóra
AU  - Kékesi, Adrienna Katalin
AU  - Karancsi, Tamás
AU  - Slegel, P
AU  - Palkovits, Miklós
AU  - Juhász, Gábor Dénes
TI  - Analysis of purine and pyrimidine bases, nucleosides and deoxynucleosides in brain microsamples (microdialysates and micropunches) and cerebrospinal fluid
JF  - NEUROCHEMISTRY INTERNATIONAL
J2  - NEUROCHEM INT
VL  - 32
PY  - 1998
IS  - 3
SP  - 247
EP  - 256
PG  - 10
SN  - 0197-0186
DO  - 10.1016/S0197-0186(97)00090-9
UR  - https://m2.mtmt.hu/api/publication/1065505
ID  - 1065505
N1  - Department of Comparative Physiology, Eötvös Loránd Univ., Múzeum krt. 4/a, H-1088 Budapest, Hungary            
            EGIS Pharmaceuticals Ltd., Keresztúri út 30-38, H-1106 Budapest, Hungary            
            Laboratory of Neuromorphology, Semmelweis Univ. Medical School, Tüzltó u. 58, H-1094 Budapest, Hungary            
            Cited By :47            
            Export Date: 2 February 2023            
            CODEN: NEUID            
            Correspondence Address: Juhasz, G.; Department of Comparative Physiology, Muzeum Ktr 4a, H-1088 Budapest, Hungary
AB  - A new chromatographic method is reported for the synchronous analysis of endogenous purine and pyrimidine bases, ribonucleosides, and deoxyribonucleosides in brain samples. An optimized gradient chromatography system with a cooled reversed-phase column allows the detection of these compounds in very low concentrations in microsamples (microdialysates and micropunches). Chromatographic peaks were identified via the retention times of known standards, with detection at two wavelengths, and also by electrospray tandem mass spectrometry, which permits the identification of certain compounds at extremely low concentrations. The method was tested on in vivo brain microdialysis samples, micropunch tissue sample and cerebrospinal fluid of rats. Extracellular concentrations of pyrimidine metabolites in brain samples and of various purine metabolites in thalamic samples are reported here first. A comparison of the results on microdialysis and cerebrospinal fluid samples suggests that the analysis of cerebrospinal fluid provides limited information on the local extracellular concentrations of these compounds. Basic dialysis experiments revealed temporarily stable baseline levels one hour after implantation of the microdialysis probes. An elevated potassium concentration in the perfusion solution caused increases in the extracellular levels of adenosine and its metabolites, and of guanosine and the pyrimidine nucleoside uridine.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Juhász, Gábor Dénes
AU  - Tarcali, J
AU  - Pungor, K
AU  - Pungor, Ernő
TI  - Electrochemical calibration of in vivo brain dialysis samplers
JF  - JOURNAL OF NEUROSCIENCE METHODS
J2  - J NEUROSCI METH
VL  - 29
PY  - 1989
IS  - 2
SP  - 131
EP  - 137
PG  - 7
SN  - 0165-0270
DO  - 10.1016/0165-0270(89)90024-1
UR  - https://m2.mtmt.hu/api/publication/1227349
ID  - 1227349
AB  - Measurement of state-related changes in the concentration of an endogenous substance using the in vivo dialysis technique requires a definition of the factors which relate the concentration detected in the outflowing perfusate to the concentration actually occurring in the extracellular space in which the sampler is located. In determining these factors, a rapid and highly accurate detector system is required which measures the concentration recovery in the perfusate and the dead space of the entire sampling system. To reduce the limitations of the microdialysis technique, two electrochemical microcells were developed for calibration of dialysis probes by computerized voltammetry. The electrochemically calibrated samplers were implanted into freely moving cats to measure concentration changes of monoamine metabolites in synchrony with sleep stages identified by polygraphy in order to demonstrate the applicability of electrochemical calibration in dialysis methods used in behavioral investigations.
LA  - English
DB  - MTMT
ER  -