@article{MTMT:112598, title = {Uridine activates fast transmembrane Ca2+ ion fluxes in rat brain homogenates}, url = {https://m2.mtmt.hu/api/publication/112598}, author = {Kardos, Julianna and Kovács, Ilona and Szárics, Éva and Kovács, Richárd and Skuban, N and Nyitrai, Gabriella and Dobolyi, Árpád and Juhász, Gábor Dénes}, doi = {10.1097/00001756-199905140-00034}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {10}, unique-id = {112598}, issn = {0959-4965}, year = {1999}, eissn = {1473-558X}, pages = {1577-1582}, orcid-numbers = {Dobolyi, Árpád/0000-0003-0397-2991; Juhász, Gábor Dénes/0000-0002-0849-6931} } @article{MTMT:1065505, title = {Analysis of purine and pyrimidine bases, nucleosides and deoxynucleosides in brain microsamples (microdialysates and micropunches) and cerebrospinal fluid}, url = {https://m2.mtmt.hu/api/publication/1065505}, author = {Dobolyi, Árpád and Reichart, Anikó and Szikra, Tamás and Szilágyi, Nóra and Kékesi, Adrienna Katalin and Karancsi, Tamás and Slegel, P and Palkovits, Miklós and Juhász, Gábor Dénes}, doi = {10.1016/S0197-0186(97)00090-9}, journal-iso = {NEUROCHEM INT}, journal = {NEUROCHEMISTRY INTERNATIONAL}, volume = {32}, unique-id = {1065505}, issn = {0197-0186}, abstract = {A new chromatographic method is reported for the synchronous analysis of endogenous purine and pyrimidine bases, ribonucleosides, and deoxyribonucleosides in brain samples. An optimized gradient chromatography system with a cooled reversed-phase column allows the detection of these compounds in very low concentrations in microsamples (microdialysates and micropunches). Chromatographic peaks were identified via the retention times of known standards, with detection at two wavelengths, and also by electrospray tandem mass spectrometry, which permits the identification of certain compounds at extremely low concentrations. The method was tested on in vivo brain microdialysis samples, micropunch tissue sample and cerebrospinal fluid of rats. Extracellular concentrations of pyrimidine metabolites in brain samples and of various purine metabolites in thalamic samples are reported here first. A comparison of the results on microdialysis and cerebrospinal fluid samples suggests that the analysis of cerebrospinal fluid provides limited information on the local extracellular concentrations of these compounds. Basic dialysis experiments revealed temporarily stable baseline levels one hour after implantation of the microdialysis probes. An elevated potassium concentration in the perfusion solution caused increases in the extracellular levels of adenosine and its metabolites, and of guanosine and the pyrimidine nucleoside uridine.}, year = {1998}, eissn = {1872-9754}, pages = {247-256}, orcid-numbers = {Dobolyi, Árpád/0000-0003-0397-2991; Kékesi, Adrienna Katalin/0000-0003-3042-4878; Palkovits, Miklós/0000-0003-0578-0387; Juhász, Gábor Dénes/0000-0002-0849-6931} } @article{MTMT:1227349, title = {Electrochemical calibration of in vivo brain dialysis samplers}, url = {https://m2.mtmt.hu/api/publication/1227349}, author = {Juhász, Gábor Dénes and Tarcali, J and Pungor, K and Pungor, Ernő}, doi = {10.1016/0165-0270(89)90024-1}, journal-iso = {J NEUROSCI METH}, journal = {JOURNAL OF NEUROSCIENCE METHODS}, volume = {29}, unique-id = {1227349}, issn = {0165-0270}, abstract = {Measurement of state-related changes in the concentration of an endogenous substance using the in vivo dialysis technique requires a definition of the factors which relate the concentration detected in the outflowing perfusate to the concentration actually occurring in the extracellular space in which the sampler is located. In determining these factors, a rapid and highly accurate detector system is required which measures the concentration recovery in the perfusate and the dead space of the entire sampling system. To reduce the limitations of the microdialysis technique, two electrochemical microcells were developed for calibration of dialysis probes by computerized voltammetry. The electrochemically calibrated samplers were implanted into freely moving cats to measure concentration changes of monoamine metabolites in synchrony with sleep stages identified by polygraphy in order to demonstrate the applicability of electrochemical calibration in dialysis methods used in behavioral investigations.}, year = {1989}, eissn = {1872-678X}, pages = {131-137}, orcid-numbers = {Juhász, Gábor Dénes/0000-0002-0849-6931} }