TY - JOUR AU - Flink, Lili Borbála AU - Ghaffarinia, Ameneh AU - Papp, Benjamin Tamás AU - Varga, Ákos AU - Vigh, András István AU - Vidács, Dániel László AU - Kui, Róbert AU - Kemény, Lajos AU - Csörgő Sándorné Bata, Zsuzsanna AU - Bozó, Renáta TI - Abnormal basement membrane results in increased keratinocyte-derived periostin expression in psoriasis similar to wound healing JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 10 SN - 2045-2322 DO - 10.1038/s41598-023-43396-0 UR - https://m2.mtmt.hu/api/publication/34177727 ID - 34177727 N1 - HCEMM-USZ Skin Research Group, University of Szeged, Szeged, 6720, Hungary AB - The psoriatic skin resembles wound healing, and it shows abnormalities at the basement membrane (BM), also in the non-lesional skin. Fibroblast-derived dermal periostin has well-known functions in wound healing and Th2-mediated diseases, such as atopic dermatitis. Here we show that serum periostin level was elevated in psoriatic patients, remarkably in the systemically treated ones. Obvious periostin positivity was detected in basal keratinocytes of the non-lesional, lesional, and previously-lesional psoriatic vs. healthy skin. Ex vivo skin models were generated to examine how different skin injuries affect periostin expression during wound healing. Our newly developed cultured salt-split model demonstrated that BM-injury induced periostin expression in basal keratinocytes, and periostin levels in the supernatant were also increased upon healing. In wound healing models, β1-integrin expression was similarly induced. β1-integrin blocking caused reduced periostin expression in in vitro scratch assay, indicating that β1-integrin can mediate periostin production. In contrast to atopic dermatitis, psoriatic basal keratinocytes are in an activated state and show a stable wound healing-like phenotype with the overexpression of periostin. This abnormal BM-induced wound healing as a potential compensatory mechanism can be initiated already in the non-lesional skin present in the lesion and keratinocytes can remain activated in the healed skin. LA - English DB - MTMT ER - TY - JOUR AU - Hernández-Quiles, Miguel AU - Martinez Campesino, Laura AU - Morris, Imogen AU - Ilyas, Zabran AU - Reynolds, Steve AU - Soon Tan, Nguan AU - Sobrevals Alcaraz, Paula AU - Stigter, Edwin C.A. AU - Varga, Ákos AU - Varga, János AU - van Es, Robert AU - Vos, Harmjan AU - Wilson, Heather L. AU - Kiss-Toth, Endre AU - Kalkhoven, Eric TI - The pseudokinase TRIB3 controls adipocyte lipid homeostasis and proliferation in vitro and in vivo JF - MOLECULAR METABOLISM J2 - MOL METAB VL - 78 PY - 2023 PG - 15 SN - 2212-8778 DO - 10.1016/j.molmet.2023.101829 UR - https://m2.mtmt.hu/api/publication/34478779 ID - 34478779 LA - English DB - MTMT ER - TY - JOUR AU - Kun-Varga, Anikó AU - Konczné Gubán, Barbara AU - Miklós, Vanda AU - Parvaneh, Shahram AU - Guba, Melinda AU - Szűcs, Diána AU - Monostori, Tamás AU - Varga, János AU - Varga, Ákos AU - Rázga, Zsolt AU - Csörgő Sándorné Bata, Zsuzsanna AU - Kemény, Lajos AU - Megyeri, Klára AU - Veréb, Zoltán TI - Herpes Simplex Virus Infection Alters the Immunological Properties of Adipose-Tissue-Derived Mesenchymal-Stem Cells JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 15 PG - 26 SN - 1661-6596 DO - 10.3390/ijms241511989 UR - https://m2.mtmt.hu/api/publication/34085614 ID - 34085614 N1 - HCEMM-SZTE Skin Research Group, University of Szeged, Hungary, TKP2021-EGA-28 AB - The proper functioning of mesenchymal stem cells (MSCs) is of paramount importance for the homeostasis of the body. Inflammation and infection can alter the function of MSCs, which can also affect the regenerative potential and immunological status of tissues. It is not known whether human herpes simplex viruses 1 and 2 (HSV1 and HSV2), well-known human pathogens that can cause lifelong infections, can induce changes in MSCs. In non-healing ulcers, HSV infection is known to affect deeper tissue layers. In addition, HSV infection can recur after initially successful cell therapies. Our aim was to study the response of adipose-derived MSCs (ADMSCs) to HSV infection in vitro. After confirming the phenotype and differentiation capacity of the isolated cells, we infected the cells in vitro with HSV1-KOS, HSV1-532 and HSV2 virus strains. Twenty-four hours after infection, we examined the gene expression of the cells via RNA-seq and RT-PCR; detected secreted cytokines via protein array; and determined autophagy via Western blot, transmission electron microscopy (TEM) and fluorescence microscopy. Infection with different HSV strains resulted in different gene-expression patterns. In addition to the activation of pathways characteristic of viral infections, distinct non-immunological pathways (autophagy, tissue regeneration and differentiation) were also activated according to analyses with QIAGEN Ingenuity Pathway Analysis, Kyoto Encyclopedia of Genes and Genome and Genome Ontology Enrichment. Viral infections increased autophagy, as confirmed via TEM image analysis, and also increased levels of the microtubule-associated protein light chain 3 (LC3B) II protein. We identified significantly altered accumulation for 16 cytokines involved in tissue regeneration and inflammation. Our studies demonstrated that HSV infection can alter the viability and immunological status of ADMSCs, which may have implications for ADMSC-based cell therapies. Alterations in autophagy can affect numerous processes in MSCs, including the inhibition of tissue regeneration as well as pathological differentiation. LA - English DB - MTMT ER - TY - JOUR AU - Varga, Ákos AU - Németh, István Balázs AU - Kemény, Lajos AU - Varga, János AU - Tiszlavicz, László AU - Kumar, D. AU - Dodd, S. AU - Simpson, A.W.M. AU - Buknicz, Tünde AU - Beynon, R. AU - Simpson, D. AU - Krenács, Tibor AU - Dockray, G.J. AU - Varro, A. TI - Elevated serum gastrin is associated with melanoma progression: putative role in increased migration and invasion of melanoma cells JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 23 PG - 16 SN - 1661-6596 DO - 10.3390/ijms242316851 UR - https://m2.mtmt.hu/api/publication/34443653 ID - 34443653 LA - English DB - MTMT ER - TY - JOUR AU - Varga, Ákos AU - Bende, Balázs AU - Baltás, Eszter AU - Németh, István Balázs AU - Varga, Erika AU - Vass, Gábor AU - Kis, Erika AU - Oláh, Judit Magdolna AU - Varga, János AU - Kocsis, Ádám László TI - Az SZTE Bőrgyógyászati és Allergológiai Klinikán a melanoma malignum sebészi kezelésében történt változások az elmúlt évtizedben [Changes in the surgical treatment of melanoma malignum at the Department of Dermatology and Allergology University of Szeged over the last decade] JF - BŐRGYÓGYÁSZATI ÉS VENEROLÓGIAI SZEMLE J2 - BVSZ VL - 99 PY - 2023 IS - 2 SP - 122 EP - 124 PG - 3 SN - 0006-7768 DO - 10.7188/bvsz.2023.99.2.6 UR - https://m2.mtmt.hu/api/publication/33928935 ID - 33928935 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Bende, Balázs AU - Degovics, Döníz AU - Varga, János AU - Varga, Ákos AU - Kocsis, Ádám László AU - Kis, Erika AU - Guba, Melinda AU - Szűcs, Diána AU - Monostori, Tamás AU - Kemény, Lajos AU - Veréb, Zoltán TI - SVF cell therapy for the treatment of non-healing limb ulcers JF - BŐRGYÓGYÁSZATI ÉS VENEROLÓGIAI SZEMLE J2 - BVSZ VL - 98 PY - 2022 IS - 4 SP - 179 EP - 180 PG - 2 SN - 0006-7768 UR - https://m2.mtmt.hu/api/publication/33190247 ID - 33190247 N1 - HCEMM-USZ Skin Research Group LA - Hungarian DB - MTMT ER - TY - JOUR AU - Flink, Lili Borbála AU - Bozó, Renáta AU - Ghaffarinia, Ameneh AU - Papp, Benjamin Tamás AU - Varga, Ákos AU - Kemény, Lajos AU - Csörgő Sándorné Bata, Zsuzsanna TI - The potential role of periostin in psoriasis JF - JOURNAL OF INVESTIGATIVE DERMATOLOGY J2 - J INVEST DERMATOL VL - 142 PY - 2022 IS - Suppl. 12 SP - S248 EP - S248 PG - 1 SN - 0022-202X DO - 10.1016/j.jid.2022.09.409 UR - https://m2.mtmt.hu/api/publication/33261241 ID - 33261241 N1 - HCEMM-USZ Skin Research Group, Szeged LA - English DB - MTMT ER - TY - JOUR AU - Flink, Lili Borbála AU - Ghaffarinia, Ameneh AU - Papp, Benjamin Tamás AU - Varga, Ákos AU - Kemény, Lajos AU - Csörgő Sándorné Bata, Zsuzsanna AU - Bozó, Renáta TI - A periostin lehetséges szerepe a pikkelysömör patogenezisében JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 14 PY - 2022 IS - 3 SP - 29 EP - 29 PG - 1 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/33190341 ID - 33190341 N1 - HCEMM-USZ Bőrgyógyászati Kutatócsoport LA - Hungarian DB - MTMT ER - TY - JOUR AU - Flink, Lili Borbála AU - Bozó, Renáta AU - Ghaffarinia, Ameneh AU - Papp, Benjamin Tamás AU - Varga, Ákos AU - Kemény, Lajos AU - Csörgő Sándorné Bata, Zsuzsanna TI - The potential role of periostin in the pathogenesis of psoriasis JF - BŐRGYÓGYÁSZATI ÉS VENEROLÓGIAI SZEMLE J2 - BVSZ VL - 98 PY - 2022 IS - 4 SP - 176 EP - 176 PG - 1 SN - 0006-7768 UR - https://m2.mtmt.hu/api/publication/33190052 ID - 33190052 N1 - HCEMM-USZ Bőrgyógyászati Kutatócsoport LA - English DB - MTMT ER - TY - JOUR AU - Kocsis, Ádám László AU - Karsko, L AU - Kurgyis, Zsuzsanna AU - Besenyi, Zsuzsanna AU - Pávics, László AU - Viharosné Dósa-Rácz, Éva AU - Kis, Erika AU - Baltás, Eszter AU - Ócsai, Henriette AU - Varga, Erika AU - Bende, Balázs AU - Varga, Ákos AU - Mohos, Gábor AU - Korom, Irma AU - Varga, János AU - Kemény, Lajos AU - Németh, István Balázs AU - Oláh, Judit Magdolna TI - Is it Necessary to Perform Sentinel Lymph Node Biopsy in Thin Melanoma?. A Retrospective Single Center Analysis TS - A Retrospective Single Center Analysis JF - PATHOLOGY AND ONCOLOGY RESEARCH J2 - PATHOL ONCOL RES VL - 26 PY - 2020 IS - 3 SP - 1861 EP - 1868 PG - 8 SN - 1219-4956 DO - 10.1007/s12253-019-00769-z UR - https://m2.mtmt.hu/api/publication/31067970 ID - 31067970 N1 - Funding Agency and Grant Number: University of Szeged (SZTE); Bolyai Scholarship Hungarian Academy of Science; NKFI (OTKA)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [K-125509, GINOP_2.3.2-15-2016-00020] Funding text: Open access funding provided by University of Szeged (SZTE). This research was supported by NKFI (OTKA) K-125509, GINOP_2.3.2-15-2016-00020 grant and Bolyai Scholarship Hungarian Academy of Science. AB - Sentinel lymph node biopsy (SLNB) is a standard procedure for regional lymph node staging and still has the most important prognostic value for the outcome of patients with thin melanoma. In addition to ulceration, SLNB had to be considered even for a single mitotic figure in thin (<1 mm) melanoma according to AJCC7th guideline, therefore, a retrospective review was conducted involving 403 pT1 melanoma patients. Among them, 152 patients suffered from pT1b ulcerated or mitotic rate ≥ 1/ mm2 melanomas according to the AJCC7th staging system. SLNB was performed in 78 cases, of which nine (11.5%) showed SLN positivity. From them, interestingly, we found a relatively high positive sentinel rate (6/78-8%) in the case of thin primary melanomas ˂0.8 mm. Moreover, the presence of regression increased the probability of sentinel positivity by 5.796 fold. After reassessing pT stage based on the new AJCC8th, 37 pT1b cases were reordered into pT1a category. There was no significant relation between other characteristics examined (age, gender, Breslow, Clark level, and mitosis index) and sentinel node positivity. Based on our data, we suggest that mitotic rate alone is not a sufficiently powerful predictor of SLN status in thin melanomas. If strict histopathological definition criteria are applied, regression might be an additional adverse feature that aids in identifying T1 patients most likely to be SLN-positive. After reassessing of pT1b cases according to AJCC8th regression proved to be independent prognostic factor on sentinel lymph node positivity. Our results propose that sentinel lymph node biopsy might also be considered at patients with regressive thin (˂0.8 mm) melanomas. LA - English DB - MTMT ER -