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It belongs to the family Circoviridae, together with porcine circovirus 1, 2 and 4. PCV-3 is distributed globally and has been detected in several European, Asian and American countries. The pathogenicity of this virus is still controversial. In this study, a published PCV-3 real-time PCR method was improved by inclusion of a parallel endogenous control assay, in order to be better suited to testing clinical samples (PCV-3/β-Actin duplex Real-Time PCR), and validated. Using this test, clinical samples from Austrian pigs were subsequently screened for the presence of PCV-3 DNA. A sensitive (limit of detection: 25 copies/PCR) and specific duplex real-time PCR for the detection of PCV-3 and β-Actin as endogenous control was established. Afterwards, 27 sera from healthy boars, as well as 30 sera and 119 tissue pools from sick pigs of different ages and 41 tissue pools from aborted pig fetuses were tested for PCV-3. Finally, 15.4 % of sera from healthy pigs, 6.7 % of sera and 12.6 % of tissue pools from sick pigs as well as 9.8 % of aborted fetuses were tested positive for PCV-3. 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