TY - JOUR AU - Leber, Rebecca AU - Heuberger, Matthias AU - Widrig, Victoria AU - Jung, Esther AU - Paux, Etienne AU - Keller, Beat AU - Sánchez-Martín, Javier TI - A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 4 SN - 0040-5752 DO - 10.1007/s00122-024-04582-4 UR - https://m2.mtmt.hu/api/publication/34817699 ID - 34817699 LA - English DB - MTMT ER - TY - JOUR AU - Xue, Shulin AU - Wang, Huan AU - Ma, Yuyu AU - Sun, Tiepeng AU - Wang, Yingxue AU - Meng, Fan AU - Wang, Xintian AU - Yang, Zihan AU - Zhang, Jieli AU - Du, Jinxuan AU - Li, Suoping AU - Li, Zhifang TI - Fine mapping of powdery mildew resistance gene PmXNM in a Chinese wheat landrace Xiaonanmai JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 2 PG - 11 SN - 0040-5752 DO - 10.1007/s00122-024-04544-w UR - https://m2.mtmt.hu/api/publication/34650975 ID - 34650975 LA - English DB - MTMT ER - TY - JOUR AU - Lavoignat, Melanie AU - Cassan, Cedric AU - Petriacq, Pierre AU - Gibon, Yves AU - Heumez, Emmanuel AU - Duque, Celine AU - Momont, Philippe AU - Rincent, Renaud AU - Blancon, Justin AU - Ravel, Catherine AU - Le Gouis, Jacques TI - Different wheat loci are associated to heritable free asparagine content in grain grown under different water and nitrogen availability JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 2 PG - 14 SN - 0040-5752 DO - 10.1007/s00122-024-04551-x UR - https://m2.mtmt.hu/api/publication/34613512 ID - 34613512 AB - Key messageDifferent wheat QTLs were associated to the free asparagine content of grain grown in four different conditions. Environmental effects are a key factor when selecting for low acrylamide-forming potential.AbstractThe amount of free asparagine in grain of a wheat genotype determines its potential to form harmful acrylamide in derivative food products. Here, we explored the variation in the free asparagine, aspartate, glutamine and glutamate contents of 485 accessions reflecting wheat worldwide diversity to define the genetic architecture governing the accumulation of these amino acids in grain. Accessions were grown under high and low nitrogen availability and in water-deficient and well-watered conditions, and plant and grain phenotypes were measured. Free amino acid contents of grain varied from 0.01 to 1.02 mg g-1 among genotypes in a highly heritable way that did not correlate strongly with grain yield, protein content, specific weight, thousand-kernel weight or heading date. Mean free asparagine content was 4% higher under high nitrogen and 3% higher in water-deficient conditions. After genotyping the accessions, single-locus and multi-locus genome-wide association study models were used to identify several QTLs for free asparagine content located on nine chromosomes. Each QTL was associated with a single amino acid and growing environment, and none of the QTLs colocalised with genes known to be involved in the corresponding amino acid metabolism. This suggests that free asparagine content is controlled by several loci with minor effects interacting with the environment. We conclude that breeding for reduced asparagine content is feasible, but should be firmly based on multi-environment field trials. Key message: Different wheat QTLs were associated to the free asparagine content of grain grown in four different conditions. Environmental effects are a key factor when selecting for low acrylamide-forming potential. LA - English DB - MTMT ER - TY - JOUR AU - Liu, Tianpeng AU - Liu, Xueying AU - He, Jihong AU - Dong, Kongjun AU - Zhang, Lei AU - Li, Yawei AU - Ren, Ruiyu AU - Yang, Tianyu TI - Comparative transcriptome analysis and genetic dissection of vegetative branching traits in foxtail millet (Setaria italica) JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 2 PG - 11 SN - 0040-5752 DO - 10.1007/s00122-023-04524-6 UR - https://m2.mtmt.hu/api/publication/34597525 ID - 34597525 AB - Key message Two major genetic loci, qTN5.1 and qAB9.1, were identified and finely mapped to the 255 Kb region with one potential candidate gene for tiller number and the 521 Kb region with eight candidate genes for axillary branch number, respectively. Vegetative branching including tillering and axillary branching are vital traits affecting both the plant architecture and the biomass in cereal crops. However, the mechanism underlying the formation of vegetative branching in foxtail millet is largely unknown. Here, a foxtail millet cultivar and its bushy wild relative Setaria viridis accession were used to construct segregating populations to identify candidate genes regulating tiller number and axillary branch number. Transcriptome analysis using vegetative branching bud samples of parental accessions was performed, and key differentially expressed genes and pathways regulating vegetative branching were pointed out. Bulk segregant analysis on their F2:3 segregating population was carried out, and a major QTL for tiller number (qTN5.1) and two major QTLs for axillary branch number (qAB2.1 and qAB9.1) were detected. Fine-mapping strategy was further performed on F2:4 segregate population, and Seita.5G356600 encoding beta-glucosidase 11 was identified as the promising candidate gene for qTN5.1, and eight genes, especially Seita.9G125300 and Seita.9G125400 annotated as B-S glucosidase 44, were finally identified as candidate genes for regulating axillary branching. Findings in this study will help to elucidate the genetic basis of the vegetative branching formation of foxtail millet and lay a foundation for breeding foxtail millet varieties with ideal vegetative branching numbers. LA - English DB - MTMT ER - TY - JOUR AU - Deng, Pingchuan AU - Du, Xin AU - Wang, Yanzhen AU - Yang, Xiaoying AU - Cheng, Xiaofang AU - Huang, Chenxi AU - Li, Tingting AU - Li, Tingdong AU - Chen, Chunhuan AU - Zhao, Jixin AU - Wang, Changyou AU - Liu, Xinlun AU - Tian, Zengrong AU - Ji, Wanquan TI - GenoBaits®WheatplusEE: a targeted capture sequencing panel for quick and accurate identification of wheat-Thinopyrum derivatives JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 2 PG - 14 SN - 0040-5752 DO - 10.1007/s00122-023-04538-0 UR - https://m2.mtmt.hu/api/publication/34595773 ID - 34595773 N1 - State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Shaanxi, Yangling, 712100, China Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Shanxi, Taiyuan, 030031, China College of Bioengineering, Yangling Vocational Technical College, Shaanxi, Yangling, 712100, China Export Date: 21 February 2024 CODEN: THAGA Correspondence Address: Ji, W.; State Key Laboratory of Crop Stress Biology in Arid Areas, Shaanxi, China; email: jiwq1319@nwafu.edu.cn AB - Thinopyrum species play a crucial role as a source of new genetic variations for enhancing wheat traits, including resistance to both abiotic and biotic factors. Accurate identification of exogenous chromosome(s) or chromosome segments or genes is essential following the introduction of alien genetic material into wheat, but this task remains challenging. This study aimed to develop a high-resolution wheat-Thinopyrum elongatum array, named GenoBaits (R) WheatplusEE, to trace alien genetic information by genotyping using a target sequencing system. This GenoBaits (R) WheatplusEE array included 90,000 capture probes derived from two species and integrated into one chip, with 10,000 and 80,000 originating from wheat and Th. elongatum, respectively. The capture probes were strategically positioned in genes and evenly distributed across the genome, facilitating the development of a roadmap for identifying each alien gene. The array was applied to the high-throughput identification of the alien chromosomes or segments in Thinopyrum and distantly related species and their derivatives. Our results demonstrated that the GenoBaits (R) WheatplusEE array could be used for direct identification of the breakpoint of alien segments, determine copy number of alien chromosomes, and reveal variations in wheat chromosomes by a single round of target sequencing of the sample. Additionally, we could efficiently and cost-effectively genotype, supporting the exploration of subgenome composition, phylogenetic relationships, and polymorphisms in essential genes (e.g., Fhb7 gene) among Thinopyrum species and their derivatives. We hope that GenoBaits (R) WheatplusEE will become a widely adopted tool for exporting wild germplasm for wheat improvement in the future. LA - English DB - MTMT ER - TY - JOUR AU - Li, Yingzheng AU - Yan, Xu AU - Cheng, Mingjun AU - Wu, Zizhou AU - Zhang, Qiyuan AU - Duan, Saifei AU - Zhou, Yong AU - Li, Huaxiong AU - Yang, Shipeng AU - Cheng, Yulin AU - Li, Wansong AU - Xu, Lulu AU - Li, Xiaofeng AU - He, Ruyu AU - Zhou, Yang AU - Yang, Chunyan AU - Iqbal, Muhammad Zafar AU - He, Jianmei AU - Rong, Tingzhao AU - Tang, Qilin TI - Genome dosage alteration caused by chromosome pyramiding and shuffling effects on karyotypic heterogeneity, reproductive diversity, and phenotypic variation in Zea-Tripsacum allopolyploids JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 1 PG - 16 SN - 0040-5752 DO - 10.1007/s00122-023-04540-6 UR - https://m2.mtmt.hu/api/publication/34672848 ID - 34672848 LA - English DB - MTMT ER - TY - JOUR AU - Norman, Michael AU - Chen, Chunhong AU - Miah, Hanif AU - Patpour, Mehran AU - Sorensen, Chris AU - Hovmoller, Mogens AU - Forrest, Kerrie AU - Kumar, Subodh AU - Prasad, Pramod AU - Gangwar, Om Prakash AU - Bhardwaj, Subhash AU - Bariana, Harbans AU - Periyannan, Sambasivam AU - Bansal, Urmil TI - Sr65: a widely effective gene for stem rust resistance in wheat JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 1 PG - 10 SN - 0040-5752 DO - 10.1007/s00122-023-04507-7 UR - https://m2.mtmt.hu/api/publication/34664834 ID - 34664834 LA - English DB - MTMT ER - TY - JOUR AU - Lin, Min AU - Islamov, Bulat AU - Aleliunas, Andrius AU - Armoniene, Rita AU - Gorash, Andrii AU - Meigas, Egon AU - Ingver, Anne AU - Tamm, Ilmar AU - Kollist, Hannes AU - Strazdina, Vija AU - Bleidere, Mara AU - Brazauskas, Gintaras AU - Lillemo, Morten TI - Genome-wide association analysis identifies a consistent QTL for powdery mildew resistance on chromosome 3A in Nordic and Baltic spring wheat JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 1 PG - 12 SN - 0040-5752 DO - 10.1007/s00122-023-04529-1 UR - https://m2.mtmt.hu/api/publication/34635713 ID - 34635713 LA - English DB - MTMT ER - TY - JOUR AU - Clouard, Camille AU - Nettelblad, Carl TI - Genotyping of SNPs in bread wheat at reduced cost from pooled experiments and imputation JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 1 PG - 15 SN - 0040-5752 DO - 10.1007/s00122-023-04533-5 UR - https://m2.mtmt.hu/api/publication/34635712 ID - 34635712 LA - English DB - MTMT ER - TY - JOUR AU - Meziadi, Chouaib AU - Alvarez-Diaz, Juan-Camilo AU - Thareau, Vincent AU - Gratias, Ariane AU - Marande, William AU - Soler-Garzon, Alvaro AU - Miklas, Phillip N. AU - Pflieger, Stephanie AU - Geffroy, Valerie TI - Fine-mapping and evolutionary history of R-BPMV, a dominant resistance gene to Bean pod mottle virus in Phaseolus vulgaris L. JF - THEORETICAL AND APPLIED GENETICS J2 - THEOR APPL GENET VL - 137 PY - 2024 IS - 1 PG - 16 SN - 0040-5752 DO - 10.1007/s00122-023-04513-9 UR - https://m2.mtmt.hu/api/publication/34620223 ID - 34620223 AB - Bean pod mottle virus (BPMV) is a comovirus that infects common bean and legumes in general. BPMV is distributed throughout the world and is a major threat on soybean, a closely related species of common bean. In common bean, BAT93 was reported to carry the R-BPMV resistance gene conferring resistance to BPMV and linked with the I resistance gene. To fine map R-BPMV, 182 recombinant inbred lines (RILs) derived from the cross BAT93 x JaloEEP558 were genotyped with polymerase chain reaction (PCR)-based markers developed using genome assemblies from G19833 and BAT93, as well as BAT93 BAC clone sequences. Analysis of RILs carrying key recombination events positioned R-BPMV to a target region containing at least 16 TIR-NB-LRR (TNL) sequences in BAT93. Because the I cluster presents a suppression of recombination and a large number of repeated sequences, none of the 16 TNLs could be excluded as R-BPMV candidate gene. The evolutionary history of the TNLs for the I cluster were reconstructed using microsynteny and phylogenetic analyses within the legume family. A single I TNL was present in Medicago truncatula and lost in soybean, mirroring the absence of complete BPMV resistance in soybean. Amplification of TNLs in the I cluster predates the divergence of the Phaseolus species, in agreement with the emergence of R-BPMV before the separation of the common bean wild centers of diversity. This analysis provides PCR-based markers useful in marker-assisted selection (MAS) and laid the foundation for cloning of R-BPMV resistance gene in order to transfer the resistance into soybean. LA - English DB - MTMT ER -