@article{MTMT:34652114, title = {Histomorphological analysis of perfusion parameters and CNS lymphatic vessels in mice: an experimental method study}, url = {https://m2.mtmt.hu/api/publication/34652114}, author = {Liu, Xiao-Ge and Hua, Qian and Peng, Tian-Tian and Chang, Ke-Xin and Deng, Chen-Geng and Zhang, Jia-Ni and Yan, Xin-Yuan and Wang, Chun-Xiang and Yan, Ke and Cai, Qing-Yuan and Tan, Yan}, doi = {10.1097/WNR.0000000000001992}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {35}, unique-id = {34652114}, issn = {0959-4965}, keywords = {immunofluorescence; paraformaldehyde; Meninges; Lymphatic Vessels; brain parenchyma}, year = {2024}, eissn = {1473-558X}, pages = {160-169}, orcid-numbers = {Liu, Xiao-Ge/0009-0000-0092-3655} } @article{MTMT:34595752, title = {Contingency learning on the proportional congruency effect of the Stroop task is manifest in deviant processing}, url = {https://m2.mtmt.hu/api/publication/34595752}, author = {Suzuki, Kota}, doi = {10.1097/WNR.0000000000001991}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {35}, unique-id = {34595752}, issn = {0959-4965}, abstract = {ObjectiveThe proportion congruency effect has been reported in interference tasks. The mostly congruent block is the frequent condition, and the mostly incongruent block is the rare condition for congruent stimuli, whereas the mostly congruent block is the rare condition, and the mostly incongruent block is the frequent condition for incongruent stimuli. This study examined the cognitive mechanism underlying the proportion congruency effect in the two-choice Stroop task using event-related potential components related to deviant processing.MethodStimuli were Kanji characters meaning 'red' and 'blue' painted by congruent and incongruent colors. Participants were required to provide a two-choice button-press response corresponding to the colors. The congruent and incongruent stimuli were presented on rare (20%) and frequent (80%) conditions.ResultsN1 was enhanced in the rare condition relative to the frequent condition for both congruent and incongruent stimuli. The results suggested that colors and characters were not processed independently in the N1 time range, which made selective attention to the relevant feature difficult. Posterior negativity from 200 to 250 ms was also different between rare and frequent conditions, indicating the presence of visual mismatch negativity for congruent and incongruent stimuli. It was considered that the distinction between congruent and incongruent stimuli was evident in mostly incongruent blocks, indicating that the selective attention mechanism was not actively engaged.ConclusionThe proportion congruency effect was explained by contingency learning rather than selective attention in the present task. The cognitive mechanisms underlying the proportion congruency effect are reflected by deviant event-related potential components.}, keywords = {Stroop task; conflict adaptation; contingency learning; deviant processing; proportion congruency effect}, year = {2024}, eissn = {1473-558X}, pages = {170-174} } @article{MTMT:34618197, title = {The severity of addiction mediates loneliness and cortical volume in internet gaming disorder}, url = {https://m2.mtmt.hu/api/publication/34618197}, author = {Dai, Junhong and Zhao, Zhen and Dong, Haohao and Du, Xiaoxia and Dong, Guang-Heng}, doi = {10.1097/WNR.0000000000001975}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {35}, unique-id = {34618197}, issn = {0959-4965}, abstract = {Internet gaming disorder (IGD) subjects reported higher loneliness scores than healthy controls. However, the neural correlates underlying the association between loneliness and IGD remain unclear. Thus, the aim of this study was to explore the relationship between loneliness, online gaming addiction and brain structure. In the current study, structural MRI data were acquired from 84 IGD subjects and 103 matched recreational game users (RGUs). We assessed and compared their addiction severity, loneliness scores, and cortical volumes and analyzed the correlations among these values. Significant correlations were found between loneliness scores and brain volumes in the postcentral cortex, the medial orbitofrontal cortex, the rostral anterior cingulate cortex, and the temporal cortex. In addition, the addiction severity scores partly mediated the relationship between loneliness score and cortical volume in IGD. The results showed that participants with extreme loneliness had significant correlations with brain regions responsible for executive control, social threat surveillance and avoidance. More importantly, the severity of addiction mediates loneliness and cortical volume. The findings shed new insight into the neural mechanisms of loneliness and IGD and have implications for potential treatment.}, keywords = {loneliness; internet gaming disorder; BRAIN STRUCTURE; Cortical volume}, year = {2024}, eissn = {1473-558X}, pages = {61-70} } @article{MTMT:34603476, title = {In-depth analyses of lncRNA and circRNA expression in the hippocampus of LPS-induced AD mice by Byu d Mar 25}, url = {https://m2.mtmt.hu/api/publication/34603476}, author = {Zhang, Yongcang and Tang, Liang and Wang, Yan and Zhu, Xiaoyan and Liu, Lan}, doi = {10.1097/WNR.0000000000001977}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {35}, unique-id = {34603476}, issn = {0959-4965}, abstract = {Byu d Mar 25 (BM25) has been verified to have neuroprotective effects in Alzheimer's disease (AD) mice. However, the molecular mechanism remains unclear. We aimed to investigate the expression profiling of lncRNAs and circRNAs by microarray analysis. Six hippocampus from LPS-mediated AD mice model treated with (normal saline (NS) (n = 3) and AD mice model treated with BM25 (n = 3) were selected. Microarray analysis was performed to detect the expression profiles of lncRNAs and circRNAs in hippocampus. Differentially expressed (DE) lncRNAs, mRNAs and circRNAs were identified through scatter plot and volcano plot filtering with a threshold of fold-change >= 2 and P <= 0.05. Co-expression network is analyzed by Circos software. Cis- and Trans- regulation were analyzed using RIsearch-2.0 and FEELNC softwares. LncRNA-transcription factors (TFs) and LncRNA-Target-TFs network were analyzed by Clusterprofiler software. The prediction of miRNAs bind to circRNAs were performed with miRNAbase. A total of 113 DElncRNAs, 117 DEmRNAs, and 4 DEcircRNAs were detected. The pathway analysis showed the mRNAs that correlated with lncRNAs were involved in apoptosis, inflammatory mediator regulation of TRP channels, NF-kappa B and PI3K-Akt signaling pathway. The lncRNA-TFs network analysis suggested the lncRNAs were mostly regulated by Ncoa1, Phf5a, Klf6, Lmx1b, and Pax3. Additionally, lncRNA-target-TFs network analysis indicated the GATA6, Junb, Smad1, Twist1, and Mafb mostly regulate the same lncRNAs: XR_001783430.1 and NR_051982.1. Furthermore, 480 miRNAs were predicted binding to 4 identified circRNAs. The BM25 may affect AD by regulating the expression of lncRNAs and circRNAs, which could regulate the expressions of mRNAs or miRNAs by LncRNA-Target-TFs network.}, keywords = {NETWORKS; Alzheimer's disease; LncRNA; circRNA; Byu d Mar 25}, year = {2024}, eissn = {1473-558X}, pages = {49-60} } @article{MTMT:33858309, title = {Interaction of the synthetic cannabinoid WIN55212 with tramadol on nociceptive thresholds and core body temperature in a chemotherapy-induced peripheral neuropathy pain model}, url = {https://m2.mtmt.hu/api/publication/33858309}, author = {Haddad, Mansour and Alsalem, Mohammad and Saleh, Tareq and Jaffal, Sahar M. and Barakat, Noor A. and El-Salem, Khalid}, doi = {10.1097/WNR.0000000000001910}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {34}, unique-id = {33858309}, issn = {0959-4965}, abstract = {Chemotherapy-induced peripheral neuropathy (CIPN) is a significant adverse effect of many anticancer drugs. Current strategies for the management of CIPN pain are still largely unmet. The aim of this study is to investigate the antinociceptive potential of combining tramadol with the synthetic cannabinoid WIN55212, and to evaluate their associated adverse effects, separately or in combination, in a CIPN rat model, and to investigate their ability to modulate the transient receptor potential vanilloid 1 (TRPV1) receptor activity. Von Frey filaments were used to determine the paw withdrawal threshold in adult male Sprague-Dawley rats (200-250 g) following intraperitoneal (i.p) injection of cisplatin. Single cell ratiometric calcium imaging was used to investigate WIN55212/tramadol combination ability to modulate the TRPV1 receptor activity. Both tramadol and WIN55212 produced dose-dependent antinociceptive effect when administered separately. The lower dose of tramadol (1 mg/kg) significantly enhanced the antinociceptive effects of WIN55212 without interfering with core body temperature. Mechanistically, capsaicin (100 nM) produced a robust increase in [Ca2+](i) in dorsal root ganglia (DRG) neurons ex vivo. Capsaicin-evoked calcium responses were significantly reduced upon pre-incubation of DRG neurons with only the highest concentration of tramadol (10 mu M), but not with WIN55212 at any concentration (0.1, 1 and 10 mu M). However, combining sub-effective doses of WIN55212 (1 mu M) and tramadol (0.1 mu M) produced a significant inhibition of capsaicin-evoked calcium responses. Combining WIN55212 with tramadol shows better antinociceptive effects with no increased risk of hypothermia, and provides a potential pain management strategy for CIPN.}, keywords = {PAIN; TRPV1; hypothermia; TRAMADOL; cisplatin; chemotherapy-induced peripheral neuropathy; WIN55212}, year = {2023}, eissn = {1473-558X}, pages = {441-448} } @article{MTMT:33965880, title = {Electroacupuncture intervention on stress-induced cardiac autonomic imbalance in rats involves corticotropin-releasing hormone system activity}, url = {https://m2.mtmt.hu/api/publication/33965880}, author = {Zhu, Li and Ye, Zhen and Zhang, Mengting and Xu, Weichen and Wang, Ruwen and Wu, Shengbing and Gao, Heren}, doi = {10.1097/WNR.0000000000001905}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {34}, unique-id = {33965880}, issn = {0959-4965}, abstract = {Autonomic imbalance is a core aspect of stress response that strongly correlates to cardiovascular diseases. Enhanced activity of the central corticotropin-releasing hormone (CRH) system may result in autonomic imbalance to cause cardiovascular responses in a stress state. Electroacupuncture at PC6 acupoints has been demonstrated to prevent and treat cardiovascular diseases. In this study, we aim to demonstrate the protective role of electroacupuncture at PC6 in ameliorating cardiac autonomic imbalance and investigate the underlying mechanisms in immobilization stress rats. Four groups were subjected. Immobilization stress was applied to three groups. And the rats in two electroacupuncture-intervened groups exerted electroacupuncture at PC6 or tail respectively. Then, we performed ECG recording for heart rate variability (HRV) analysis, and rats were sacrificed after experiments for biological analysis. HRV analysis indicated that electroacupuncture at PC6 improved the enhanced low-frequency band of the power spectrum (LF), the reduced high-frequency band of the power spectrum (HF), and the enhanced LF/HF ratio caused by immobilization stress. Besides, electroacupuncture at PC6 significantly decreased phosphorylated tyrosine hydroxylase expression and increased acetylcholine esterase expression in heart of immobilization stress rats. Furthermore, electroacupuncture at PC6 significantly decreased CRH level and CRH 1 type receptor and CRH 2 type receptor (CRHR2) expressions in the rostral ventrolateral medulla (RVLM), and CRH level and CRHR2 expression in the nucleus of the solitary tract (NTS) of immobilization stress rats. Our findings suggest that electroacupuncture at PC6 can ameliorate stress-induced cardiac autonomic imbalance by modulating the CRHergic input in the RVLM and NTS.}, keywords = {CORTICOTROPIN-RELEASING HORMONE; ROSTRAL VENTROLATERAL MEDULLA; STRESS; Nucleus of the solitary tract; cardiac autonomic activity; PC6}, year = {2023}, eissn = {1473-558X}, pages = {401-410} } @article{MTMT:33649790, title = {KCNQ4 potassium channel subunit deletion leads to exaggerated acoustic startle reflex in mice}, url = {https://m2.mtmt.hu/api/publication/33649790}, author = {Maamrah, Baneen and Deák-Pocsai, Krisztina and Bayasgalan, Tsogbadrakh and Csemer , Andrea and Pál, Balázs Zoltán}, doi = {10.1097/WNR.0000000000001883}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {34}, unique-id = {33649790}, issn = {0959-4965}, abstract = {The potassium voltage-gated channel subfamily Q member 4 (KCNQ4) subunit forms channels responsible for M-current, a muscarine-sensitive potassium current regulating neuronal excitability. In contrast to other KCNQ subunits, its expression is restricted to the cochlear outer hair cells, the auditory brainstem and other brainstem nuclei in a great overlap with structures involved in startle reflex. We aimed to show whether startle reflexis affected by the loss of KCNQ4 subunit and whether these alterations are similar to the ones caused by brainstem hyperexcitability. Young adult KCNQ4 knockout mice and wild-type littermates, as well as mice expressing hM3D chemogenetic actuator in the pontine caudal nucleus and neurons innervating it were used for testing acoustic startle. The acoustic startle reflex was significantly increased in knockout mice compared with wild-type littermates. When mice expressing human M3 muscarinic (hM3D) in nuclei related to startle reflex were tested, a similar increase of the first acoustic startle amplitude and a strong habituation of the further responses was demonstrated. We found that the acoustic startle reflex is exaggerated and minimal habituation occurs in KCNQ4 knockout animals. These changes are distinct from the effects of the hyperexcitability of nuclei involved in startle. One can conclude that the exaggerated startle reflex found with the KCNQ4 subunit deletion is the consequence of both the cochlear damage and the changes in neuronal excitability of startle networks.}, keywords = {potassium channel; Auditory system; hyperexcitability; KCNQ4; Acoustic startle reflex; designer receptor exclusively activated by designer drugs (DREADD)}, year = {2023}, eissn = {1473-558X}, pages = {232-237} } @article{MTMT:33861956, title = {Filial imprinting in domestic chicks; cytoplasmic polyadenylation element binding protein 3, predispositions and learning}, url = {https://m2.mtmt.hu/api/publication/33861956}, author = {Chitadze, Lela and Tevdoradze, Ekaterine and Kiguradze, Tamar and McCabe, Brian J. and Solomonia, Revaz}, doi = {10.1097/WNR.0000000000001872}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {34}, unique-id = {33861956}, issn = {0959-4965}, abstract = {Visual imprinting is a learning process, whereby young animals come to prefer a visual stimulus after exposure to it (training). Available evidence indicates that the intermediate medial mesopallium (IMM) in the domestic chick forebrain is a site of memory formation during visual imprinting. We have found previously that cytoplasmic polyadenylation element binding protein 3 in the P2 plasma membrane-mitochondrial fraction (CPEB3-P2) is upregulated in a learning-dependent way in the left IMM 24 h after training. CPEB3 has two forms, soluble and aggregated. Soluble CPEB3 represses translation; the aggregated form (CPEB3-AF) is amyloid-like and can promote translation. Our previous study did not show which of these two forms is increased after imprinting. We have now resolved this matter by measuring, 24 h after training, CPEB3-P2 and CPEB3-AF in the IMM and a control brain region, the posterior pole of nidopallium (PPN). The methods include imprinting training with a visual stimulus, behavioral measurement of preference, preparation of aggregated CPEB3, western immunoblotting, quantitation of proteins, statistical linear modeling. Only in the left IMM were the level of CPEB3-AF and learning strength correlated, increased CPEB3-AF level reflecting a predisposition to learn readily. CPEB3-P2 level also increased with learning strength in the left IMM, but as a result of learning. No correlations were detected in the right IMM or PPN. We propose two separate systems, both modulating synaptic strength through control of local translation. They are represented by CPEB3-AF (associated with a predisposition to learn) and soluble CPEB3 (associated with learning itself).}, keywords = {learning; RECOGNITION MEMORY; PREDISPOSITION; prion-like; Intermediate medial mesopallium; cytoplasmic polyadenylation element binding protein 3; IMM}, year = {2023}, eissn = {1473-558X}, pages = {144-149} } @article{MTMT:33935538, title = {SNARE protein VAMP-2, but not syntaxin-1, SNAP-25 and synaptotagmin 1, expressed in perisynaptic astrocytic processes in the CA1 area of the rat hippocampus}, url = {https://m2.mtmt.hu/api/publication/33935538}, author = {Hussain, Suleman and Davanger, Svend}, doi = {10.1097/WNR.0000000000001861}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {34}, unique-id = {33935538}, issn = {0959-4965}, abstract = {ObjectivePerisynaptic astrocytic processes have been suggested as sites for the regulated release of neuroactive substances. However, very little is known about the molecular properties of regulated exocytosis in these processes. Soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) proteins mediate synaptic vesicle exocytosis from neuronal cells and might be candidates for regulated exocytosis also from astrocytic processes. The expression of SNARE proteins in astrocytes, however, is not clarified. Thus, we aimed to investigate the localization and relative concentrations of neuronal SNARE proteins syntaxin-1, synaptosomal nerve-associated protein 25 (SNAP-25), vesicle-associated membrane protein 2 (VAMP-2) (synaptobrevin-2) and calcium sensor synaptotagmin 1 in perisynaptic astrocytic processes compared to nerve terminals and dendrites. MethodsWe used quantitative immunogold electron microscopy of the rat hippocampus to investigate the localization and concentration of neuronal SNARE proteins. ResultsAs expected, analysis of the immunogold data revealed a lower labeling density of SNARE proteins in the perisynaptic astrocytic processes than in presynaptic terminals. The same was also true when compared to dendrites. Contrary to VAMP-2, labeling intensities for syntaxin-1, SNAP-25 and synaptotagmin 1 were not distinguishable from background labeling in the processes. The relative concentration of VAMP-2 stands out, as the mean perisynaptic astrocytic process concentration of the protein was only 68 % lower than in presynaptic terminals and still 32 % higher than in dendrites. VAMP-2 was associated with small vesicles in the processes. Some gold particles were located over the astrocytic plasma membrane. ConclusionVAMP-2 is expressed in perisynaptic astrocytic processes, with a concentration higher than in the dendrites. Our results are compatible with the role of VAMP-2 in exocytosis from perisynaptic astrocytic processes.}, keywords = {electron microscopy; SNARE Proteins; tripartite synapse; synaptotagmin 1; perisynaptic astrocytic processes}, year = {2023}, eissn = {1473-558X}, pages = {75-80} } @article{MTMT:33617749, title = {Knockout of transient receptor potential ankyrin 1 (TRPA1) modulates the glial phenotype and alleviates perihematomal neuroinflammation after intracerebral hemorrhage in mice via MAPK/NF-kappa B signaling}, url = {https://m2.mtmt.hu/api/publication/33617749}, author = {Xia, Min and Chen, Yu-Jie and Chen, Beike and Ru, Xufang and Wang, Jie and Lin, Jie and Tang, Xiaoqin and Chen, Weixiang and Hu, Rong and Li, Weina and Feng, Hua}, doi = {10.1097/WNR.0000000000001862}, journal-iso = {NEUROREPORT}, journal = {NEUROREPORT}, volume = {34}, unique-id = {33617749}, issn = {0959-4965}, abstract = {The objective is to explore the role of astrocytic transient receptor potential ankyrin 1 (TRPA1) in glial phenotype transformation in neuroinflammation after intracerebral hemorrhage (ICH). Wild-type astrocytes and TRPA1(-/-) astrocytes were subjected to 6-h hemin treatment, and the calcium ions and transcriptome sequencing were assessed. A mouse autologous blood injection ICH model was established to evaluate the proliferation and phenotypes of astrocytes and microglia around the hematoma. The neuroinflammation and behavioral performance of wild-type ICH mice and TRPA1(-/-) ICH mice were assessed. Knockout of astrocytic TRPA1 decreased calcium ions of astrocytes after hemin treatment in-vitro, and microglial and astrocytes around the hematoma proliferated after the ICH model. Furthermore, RNA-sequencing (RNA-seq), immunofluorescence, and Western blotting results showed that the activated astrocytes transformed into the A2 phenotype in TRPA1(-/-) ICH mice. The 'ameboid' microglia were observed around the hematoma in TRPA1(-/-) ICH mice. The proliferation of A2 astrocytes and 'ameboid' microglia ameliorated the neuroinflammation after ICH. The inflammatory response was reduced by inhibiting the mitogen-activated protein kinase/nuclear factor kappa-B signaling pathway, and neurologic deficits were improved in TRPA1(-/-) ICH mice compared with wild-type ICH mice. This research suggests that astrocytic TRPA1 is a new therapeutic target to rescue neuroinflammation by modulating the glial phenotype after ICH.}, keywords = {ACTIVATION; neuroinflammation; microglia; ASTROCYTE; Intracerebral hemorrhage}, year = {2023}, eissn = {1473-558X}, pages = {81-92} }