TY - JOUR AU - Fujibayashi, M. AU - Abe, K. TI - A behavioral analysis system MCFBM enables objective inference of songbirds’ attention during social interactions JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 9 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100844 UR - https://m2.mtmt.hu/api/publication/35622954 ID - 35622954 N1 - Export Date: 5 December 2024 Correspondence Address: Abe, K.; Lab of Brain Development, Katahira 2-1-1, Aoba-ku, Miyagi, Japan; email: k.abe@tohoku.ac.jp LA - English DB - MTMT ER - TY - JOUR AU - Chi, Y. AU - Lu, X. AU - Li, S. AU - Wang, J. AU - Xi, J. AU - Zhou, X. AU - Tang, C. AU - Chen, M. AU - Yuan, H. AU - Lin, S. AU - Xiao, Y. AU - Lai, L. AU - Zou, Q. TI - A compact, versatile drug-induced splicing switch system with minimal background expression JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 9 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100842 UR - https://m2.mtmt.hu/api/publication/35611056 ID - 35611056 N1 - Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, 529020, China Jiangmen Wuyi Traditional Chinese Medicine Hospital, Jiangmen, 529000, China CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China Export Date: 29 November 2024 Correspondence Address: Lai, L.; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, China; email: lai_liangxue@gibh.ac.cn Correspondence Address: Zou, Q.; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, China; email: zouqj@wyu.edu.cn AB - Gene-switch techniques hold promising applications in contemporary genetics research, particularly in disease treatment and genetic engineering. Here, we developed a compact drug-induced splicing system that maintains low background using a human ubiquitin C (hUBC) promoter and optimized drug (LMI070) binding sequences based on the Xon switch system. To ensure precise subcellular localization of the protein of interest (POI), we inserted a 2A self-cleaving peptide between the extra N-terminal peptide and POI. This streamlined and optimized switch system, named miniXon2G, effectively regulated POIs in different subcellular localizations both in vitro and in vivo. Furthermore, miniXon2G could be integrated into endogenous gene loci, resulting in precise, reversible regulation of target genes by both endogenous regulators and drugs. Overall, these findings highlight the performance of miniXon2G in controlling protein expression with great potential for general applicability to diverse biological scenarios requiring precise and delicate regulation. © 2024 The Authors LA - English DB - MTMT ER - TY - JOUR AU - Wu, Zhenqin AU - Kondo, Ayano AU - McGrady, Monee AU - Baker, Ethan A. G. AU - Chidester, Benjamin AU - Wu, Eric AU - Rahim, Maha K. AU - Bracey, Nathan A. AU - Charu, Vivek AU - Cho, Raymond J. AU - Cheng, Jeffrey B. AU - Afkarian, Maryam AU - Zou, James AU - Mayer, Aaron T. AU - Trevino, Alexandro E. TI - Discovery and generalization of tissue structures from spatial omics data JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 8 PG - 23 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100838 UR - https://m2.mtmt.hu/api/publication/35332823 ID - 35332823 LA - English DB - MTMT ER - TY - JOUR AU - Dyer, Brannen AU - Yu, Sue O. AU - Brown, R. Lane AU - Lang, Richard A. AU - D'Souza, Shane P. TI - Defining spatial nonuniformities of all ipRGC types using an improved Opn4cre recombinase mouse line JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 8 PG - 22 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100837 UR - https://m2.mtmt.hu/api/publication/35289020 ID - 35289020 LA - English DB - MTMT ER - TY - JOUR AU - Odriozola, Inaki AU - Rasmussen, Jacob A. AU - Gilbert, M. Thomas P. AU - Limborg, Morten T. AU - Alberdi, Antton TI - A practical introduction to holo-omics JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 7 PG - 12 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100820 UR - https://m2.mtmt.hu/api/publication/35381110 ID - 35381110 LA - English DB - MTMT ER - TY - JOUR AU - Shoyer, T. Curtis AU - Collins, Kasie L. AU - Ham, Trevor R. AU - Blanchard, Aaron T. AU - Malavade, Juilee N. AU - Johns, Benjamin A. AU - West, Jennifer L. AU - Hoffman, Brenton D. TI - Detection of fluorescent protein mechanical switching in cellulo JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 7 PG - 25 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100815 UR - https://m2.mtmt.hu/api/publication/35293045 ID - 35293045 LA - English DB - MTMT ER - TY - JOUR AU - Bartelt, Luke C. AU - Fakhri, Mouad AU - Adamek, Grazyna AU - Trybus, Magdalena AU - Samelak-Czajka, Anna AU - Jackowiak, Paulina AU - Fiszer, Agnieszka AU - Lowe, Craig B. AU - Spada, Albert R. La AU - Switonski, Pawel M. TI - Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 7 PG - 15 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100816 UR - https://m2.mtmt.hu/api/publication/35279761 ID - 35279761 LA - English DB - MTMT ER - TY - JOUR AU - Xie, Jiajing AU - Chen, Ying AU - Luo, Shijie AU - Yang, Wenxian AU - Lin, Yuxiang AU - Wang, Liansheng AU - Ding, Xin AU - Tong, Mengsha AU - Yu, Rongshan TI - Tracing unknown tumor origins with a biological-pathway-based transformer model JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 6 PG - 18 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100797 UR - https://m2.mtmt.hu/api/publication/35350884 ID - 35350884 LA - English DB - MTMT ER - TY - JOUR AU - Kowalczewski, Andrew AU - Sun, Shiyang AU - Mai, Nhu Y. AU - Song, Yuanhui AU - Hoang, Plansky AU - Liu, Xiyuan AU - Yang, Huaxiao AU - Ma, Zhen TI - Design optimization of geometrically confined cardiac organoids enabled by machine learning techniques JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 6 PG - 19 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100798 UR - https://m2.mtmt.hu/api/publication/35324298 ID - 35324298 LA - English DB - MTMT ER - TY - JOUR AU - Nouri, Nima AU - Gaglia, Giorgio AU - Mattoo, Hamid AU - de Rinaldis, Emanuele AU - Savova, Virginia TI - GENIX enables comparative network analysis of single-cell RNA sequencing to reveal signatures of therapeutic interventions JF - CELL REPORTS METHODS J2 - CELL REP METH VL - 4 PY - 2024 IS - 6 PG - 20 SN - 2667-2375 DO - 10.1016/j.crmeth.2024.100794 UR - https://m2.mtmt.hu/api/publication/35313362 ID - 35313362 LA - English DB - MTMT ER -