@article{MTMT:35622954, title = {A behavioral analysis system MCFBM enables objective inference of songbirds’ attention during social interactions}, url = {https://m2.mtmt.hu/api/publication/35622954}, author = {Fujibayashi, M. and Abe, K.}, doi = {10.1016/j.crmeth.2024.100844}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35622954}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375} } @article{MTMT:35611056, title = {A compact, versatile drug-induced splicing switch system with minimal background expression}, url = {https://m2.mtmt.hu/api/publication/35611056}, author = {Chi, Y. and Lu, X. and Li, S. and Wang, J. and Xi, J. and Zhou, X. and Tang, C. and Chen, M. and Yuan, H. and Lin, S. and Xiao, Y. and Lai, L. and Zou, Q.}, doi = {10.1016/j.crmeth.2024.100842}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35611056}, issn = {2667-2375}, abstract = {Gene-switch techniques hold promising applications in contemporary genetics research, particularly in disease treatment and genetic engineering. Here, we developed a compact drug-induced splicing system that maintains low background using a human ubiquitin C (hUBC) promoter and optimized drug (LMI070) binding sequences based on the Xon switch system. To ensure precise subcellular localization of the protein of interest (POI), we inserted a 2A self-cleaving peptide between the extra N-terminal peptide and POI. This streamlined and optimized switch system, named miniXon2G, effectively regulated POIs in different subcellular localizations both in vitro and in vivo. Furthermore, miniXon2G could be integrated into endogenous gene loci, resulting in precise, reversible regulation of target genes by both endogenous regulators and drugs. Overall, these findings highlight the performance of miniXon2G in controlling protein expression with great potential for general applicability to diverse biological scenarios requiring precise and delicate regulation. © 2024 The Authors}, keywords = {Animals; Adult; Male; Humans; MICE; GENETICS; ARTICLE; MOUSE; GENOMICS; human; animal; gene locus; Genetic engineering; protein localization; controlled study; nonhuman; animal tissue; animal experiment; regulatory mechanism; drug effect; in vitro study; cellular distribution; RNA Splicing; RNA Splicing; Promoter Regions, Genetic; human cell; protein expression; in vivo study; promoter region; gene control; drug binding; HEK293 Cells; gene cassette; HEK293 cell line; gene switching; ubiquitin C; CP: Molecular biology; CP: Biotechnology; ◼; branaplam}, year = {2024}, eissn = {2667-2375} } @article{MTMT:35332823, title = {Discovery and generalization of tissue structures from spatial omics data}, url = {https://m2.mtmt.hu/api/publication/35332823}, author = {Wu, Zhenqin and Kondo, Ayano and McGrady, Monee and Baker, Ethan A. G. and Chidester, Benjamin and Wu, Eric and Rahim, Maha K. and Bracey, Nathan A. and Charu, Vivek and Cho, Raymond J. and Cheng, Jeffrey B. and Afkarian, Maryam and Zou, James and Mayer, Aaron T. and Trevino, Alexandro E.}, doi = {10.1016/j.crmeth.2024.100838}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35332823}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375} } @article{MTMT:35289020, title = {Defining spatial nonuniformities of all ipRGC types using an improved Opn4cre recombinase mouse line}, url = {https://m2.mtmt.hu/api/publication/35289020}, author = {Dyer, Brannen and Yu, Sue O. and Brown, R. Lane and Lang, Richard A. and D'Souza, Shane P.}, doi = {10.1016/j.crmeth.2024.100837}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35289020}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375} } @article{MTMT:35381110, title = {A practical introduction to holo-omics}, url = {https://m2.mtmt.hu/api/publication/35381110}, author = {Odriozola, Inaki and Rasmussen, Jacob A. and Gilbert, M. Thomas P. and Limborg, Morten T. and Alberdi, Antton}, doi = {10.1016/j.crmeth.2024.100820}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35381110}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375}, orcid-numbers = {Alberdi, Antton/0000-0002-2875-6446} } @article{MTMT:35293045, title = {Detection of fluorescent protein mechanical switching in cellulo}, url = {https://m2.mtmt.hu/api/publication/35293045}, author = {Shoyer, T. Curtis and Collins, Kasie L. and Ham, Trevor R. and Blanchard, Aaron T. and Malavade, Juilee N. and Johns, Benjamin A. and West, Jennifer L. and Hoffman, Brenton D.}, doi = {10.1016/j.crmeth.2024.100815}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35293045}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375} } @article{MTMT:35279761, title = {Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue}, url = {https://m2.mtmt.hu/api/publication/35279761}, author = {Bartelt, Luke C. and Fakhri, Mouad and Adamek, Grazyna and Trybus, Magdalena and Samelak-Czajka, Anna and Jackowiak, Paulina and Fiszer, Agnieszka and Lowe, Craig B. and Spada, Albert R. La and Switonski, Pawel M.}, doi = {10.1016/j.crmeth.2024.100816}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35279761}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375} } @article{MTMT:35350884, title = {Tracing unknown tumor origins with a biological-pathway-based transformer model}, url = {https://m2.mtmt.hu/api/publication/35350884}, author = {Xie, Jiajing and Chen, Ying and Luo, Shijie and Yang, Wenxian and Lin, Yuxiang and Wang, Liansheng and Ding, Xin and Tong, Mengsha and Yu, Rongshan}, doi = {10.1016/j.crmeth.2024.100797}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35350884}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375}, orcid-numbers = {Lin, Yuxiang/0000-0001-8854-2995} } @article{MTMT:35324298, title = {Design optimization of geometrically confined cardiac organoids enabled by machine learning techniques}, url = {https://m2.mtmt.hu/api/publication/35324298}, author = {Kowalczewski, Andrew and Sun, Shiyang and Mai, Nhu Y. and Song, Yuanhui and Hoang, Plansky and Liu, Xiyuan and Yang, Huaxiao and Ma, Zhen}, doi = {10.1016/j.crmeth.2024.100798}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35324298}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375}, orcid-numbers = {Ma, Zhen/0000-0001-5228-105X} } @article{MTMT:35313362, title = {GENIX enables comparative network analysis of single-cell RNA sequencing to reveal signatures of therapeutic interventions}, url = {https://m2.mtmt.hu/api/publication/35313362}, author = {Nouri, Nima and Gaglia, Giorgio and Mattoo, Hamid and de Rinaldis, Emanuele and Savova, Virginia}, doi = {10.1016/j.crmeth.2024.100794}, journal-iso = {CELL REP METH}, journal = {CELL REPORTS METHODS}, volume = {4}, unique-id = {35313362}, issn = {2667-2375}, year = {2024}, eissn = {2667-2375}, orcid-numbers = {Savova, Virginia/0000-0002-5387-4161} }