TY - JOUR AU - Ruan, Zhong-bao AU - Wang, Fei AU - Chen, Ge-cai AU - Zhu, Jun-guo AU - Ren, Yin AU - Zhu, Li TI - Comparison of cardiac function and structure after left atrial appendage occlusion without versus with ablation in patients with non-valvular atrial fibrillation: a retrospective study JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 9 SP - 1710 EP - 1717 PG - 8 SN - 1449-1907 DO - 10.7150/ijms.95080 UR - https://m2.mtmt.hu/api/publication/35329847 ID - 35329847 LA - English DB - MTMT ER - TY - JOUR AU - Sun, Yi-Hung AU - Wang, Ching-Ming AU - Shen, Huang-Pin AU - Lee, Chung-Yuan AU - Lin, Chiao-Wen AU - Yang, Shun-Fa AU - Wang, Po-Hui TI - Impact of CD44 genetic variants on clinicopathological characteristics of uterine cervical cancer patients JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 8 SP - 1428 EP - 1437 PG - 10 SN - 1449-1907 DO - 10.7150/ijms.96414 UR - https://m2.mtmt.hu/api/publication/35223287 ID - 35223287 LA - English DB - MTMT ER - TY - JOUR AU - Yang, J. AU - Fan, L.-Y. AU - Shi, K.-Y. TI - Integrated Single-cell and Transcriptome Sequencing Analyses Identified PREX1 as an Immune-related Prognostic Biomarker for Liver Hepatocellular Carcinoma JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 8 SP - 1559 EP - 1574 PG - 16 SN - 1449-1907 DO - 10.7150/ijms.94812 UR - https://m2.mtmt.hu/api/publication/35071430 ID - 35071430 N1 - Department of Diagnostic Ultrasound Imaging & Interventional Therapy, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, 310022, China Department of Radiology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Zhejiang, Hangzhou, 310022, China Export Date: 26 June 2024 Correspondence Address: Shi, K.-Y.; Department of Diagnostic Ultrasound Imaging & Interventional Therapy, China; email: shiky@zjcc.org.cn Funding text 1: We acknowledge TCGA, UALCAN and TNM plotter for providing meaningful datasets. This study was supported by the Project of Zhejiang Provincial Department of Health (2021KY085). Funding text 2: This study was supported by the Project of Zhejiang Provincial Department of Health (2021KY085). AB - Background: PtdIns (3,4,5) P3-dependent Rac exchanger 1 (PREX1), also known as PREX1, a member of the Rac guanine nucleotide exchange factors (Rac-GEF) family. Studies have suggested that PREX1 plays a role in mediating oncogenic pathway activation and controlling various biological mechanisms in different types of cancer, including liver hepatocellular carcinoma (LIHC). However, the function of PREX1 in the pathogenesis of LIHC and its potential role on immunological regulation is not clearly elucidated. Methods: The expression level and the clinical role of PREX1 in LIHC was analyzed based on database from the Cancer Genome Atlas (TCGA), TNM plotter and University of Alabama Cancer Database (UALCAN). We investigated the relationship between PREX1 and immunity in LIHC by TISIDB, CIBERSORT and single cell analysis. Immunotherapy responses were assessed by the immunophenoscores (IPS). Moreover, biological functional assays were performed to further investigate the roles of PREX1 in liver cancer cell lines. Results: Higher expression of PREX1 in LIHC tissues than in normal liver tissues was found based on public datasets. Further analysis revealed that PREX1 was associated with worse clinical characteristics and dismal prognosis. Pathway enrichment analysis indicated that PREX1 participated in immune-related pathways. Through CIBERSORT and single cell analysis, we found a remarkable correlation between the expression of PREX1 and various immune cells, especially macrophages. In addition, high PREX1 expression was found to be associated with a stronger response to immunotherapy. Furthermore, in vitro assays indicated that depletion of PREX1 can suppress invasion and proliferation of LIHC cells. Conclusion: Elevated expression of PREX1 indicates poor prognosis, influences immune modulation and predicts sensitivity of immunosuppression therapy in LIHC. Our results suggested that PREX1 may be a prognostic biomarker and therapeutic target, offering new treatment options for LIHC. © The author(s). LA - English DB - MTMT ER - TY - JOUR AU - Chen, Xiaoli AU - Li, Aihua AU - Kuang, Yuanyuan AU - Ma, Qilin TI - Gastroesophageal reflux disease and atrial fibrillation: a bidirectional Mendelian randomization study JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 7 SP - 1321 EP - 1328 PG - 8 SN - 1449-1907 DO - 10.7150/ijms.95518 UR - https://m2.mtmt.hu/api/publication/35290778 ID - 35290778 LA - English DB - MTMT ER - TY - JOUR AU - Liu, Yang AU - Xiao, Tianci AU - Wang, Zili AU - Ou, Yangbin AU - Tan, Ying AU - Chen, Liting AU - Zhou, Na AU - Zou, Rongjun TI - A circular network of adenosine-mediated mitochondrial dysfunction as coregulators of acute myocardial infarction JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 7 SP - 1353 EP - 1365 PG - 13 SN - 1449-1907 DO - 10.7150/ijms.97066 UR - https://m2.mtmt.hu/api/publication/34960335 ID - 34960335 LA - English DB - MTMT ER - TY - JOUR AU - Liu, R. AU - Wang, Y. AU - Bu, J. AU - Li, Q. AU - Chen, F. AU - Zhu, M. AU - Chi, H. AU - Yu, G. AU - Zhu, T. AU - Zhu, X. AU - Zhao, G. TI - Construction and Validation of Novel Ferroptosis-related Risk Score Signature and Prognostic Prediction Nomogram for Patients with Colorectal Cancer JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 6 SP - 1103 EP - 1116 PG - 14 SN - 1449-1907 DO - 10.7150/ijms.91446 UR - https://m2.mtmt.hu/api/publication/35258963 ID - 35258963 N1 - Department of General Surgery, Cancer Hospital of Dalian University of Technology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Liaoning, Shenyang, 110042, China Liaoning University of Traditional Chinese Medicine, Liaoning, Shenyang, 110847, China Department of Colorectal Surgery, Shengjing Hospital of China Medical University, Liaoning, Shenyang, 110004, China Department of Endoscopy, Cancer Hospital of Dalian University of Technology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Liaoning, Shenyang, 110042, China Department of Gynecology, People’s Hospital of Liaoning Province, Liaoning, Shenyang, 110016, China Department of Breast Surgery, Panjin Central Hospital, Liaoning, Panjin, 124010, China Liaoning Provincial Key Laboratory of Precision Medicine for Malignant Tumors, Liaoning, Shenyang, 110042, China Markey Cancer Center, University of Kentucky, Lexington, KY 40536, United States Export Date: 11 September 2024 Correspondence Address: Zhu, T.; Department of Breast Surgery, Liaoning, China; email: jimmiezhu@yeah.net Correspondence Address: Zhu, X.; Department of General Surgery, Liaoning, China; email: XudongZhu@uky.edu Correspondence Address: Zhao, G.; Department of General Surgery, Liaoning, China; email: zhaoguohua1975@hotmail.com Chemicals/CAS: phospholipid hydroperoxide glutathione peroxidase, 97089-70-8; CDKN2A protein, human; SLC7A11 protein, human AB - Background: Colorectal cancer (CRC) has a high morbidity and mortality. Ferroptosis is a phenomenon in which metabolism and cell death are closely related. The role of ferroptosis-related genes in the progression of CRC is still not clear. Therefore, we screened and validated the ferroptosis-related genes which could determine the prevalence, risk and prognosis of patients with CRC. Methods: We firstly screened differentially expressed ferroptosis-related genes by The Cancer Genome Atlas (TCGA) database. Then, these genes were used to construct a risk-score model using the least absolute shrinkage and selection operator (LASSO) regression algorithm. The function and prognosis of the ferroptosis-related genes were confirmed using multi-omics analysis. The gene expression results were validated using publicly available databases and qPCR. We also used publicly available data and ferroptosis-related genes to construct a prognostic prediction nomogram. Results: A total of 24 differential expressed genes associated with ferroptosis were screened in this study. A three-gene risk score model was then established based on these 24 genes and GPX3, CDKN2A and SLC7A11 were selected. The significant prognostic value of this novel three-gene signature was also assessed. Furthermore, we conducted RT-qPCR analysis on cell lines and tissues, and validated the high expression of CDKN2A, GPX3 and low expression of SLC7A11 in CRC cells. The observed mRNA expression of GPX3, CDKN2A and SLC7A11 was consistent with the predicted outcomes. Besides, eight variables including selected ferroptosis related genes were included to establish the prognostic prediction nomogram for patients with CRC. The calibration plots showed favorable consistency between the prediction of the nomogram and actual observations. Also, the time-dependent AUC (>0.7) indicated satisfactory discriminative ability of the nomogram. Conclusions: The present study constructed and validated a novel ferroptosis-related three-gene risk score signature and a prognostic prediction nomogram for patients with CRC. Also, we screened and validated the ferroptosis-related genes GPX3, CDKN2A, and SLC7A11 which could serve as novel biomarkers for patients with CRC. © The author(s). LA - English DB - MTMT ER - TY - JOUR AU - He, Y. AU - Wasti, B. AU - Yuan, Y. AU - Chen, Z. AU - Duan, W. AU - Jia, J. AU - Xiao, B. AU - Zhang, X. AU - Li, J. AU - Zeng, Q. AU - Ma, L. AU - Liu, S. AU - Xiang, X. TI - Combination of androgen and estrogen improves asthma by mediating Runx3 expression JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 6 SP - 1003 EP - 1015 PG - 13 SN - 1449-1907 DO - 10.7150/ijms.91253 UR - https://m2.mtmt.hu/api/publication/35119518 ID - 35119518 N1 - Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital, Central South University, 139 Middle Renmin Road, Hunan, Changsha, 410011, China Department of Emergency Medicine, The Second Xiangya Hospital, Central South University, Emergency and Difficult Diseases Institute of Central, South University, 139 Middle Renmin Road, Hunan, Changsha, 410011, China Department of Respiratory Medicine, The Second Affiliated Hospital of Hainan Medical University, 48 Pak Shui Tong Road, Hainan, Haikou, 570000, China Department of Respiratory and Critical Care Medicine, Hunan Provincial People's Hospital, 61 West Jiefang Road, Hunan, Changsha, 410005, China Department of Respiratory and Critical Care Medicine, Longshan County People’s Hospital, Hunan, Longshan, 416800, China Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Guilin Medical University, 15 Le Qun Road, Guangxi, Guilin, 541001, China Export Date: 11 July 2024 Correspondence Address: Xiang, X.139 Middle Renmin Road, Hunan, China; email: xudongxiang@csu.edu.cn Chemicals/CAS: Runx3 protein, human; Runx3 protein, mouse Funding details: National Natural Science Foundation of China, NSFC, 82170039, 82160009 Funding details: National Natural Science Foundation of China, NSFC Funding details: Natural Science Foundation of Hunan Province, 2020JJ4815 Funding details: Natural Science Foundation of Hunan Province Funding text 1: This study was supported by the National Natural Science Foundation of China (No. 82170039, No. 82160009) and the Natural Science Foundation of Hunan Province (No. 2020JJ4815). AB - Objective: Asthma is a chronic heterogeneous airway disease, and imbalanced T-helper type 1 (Th1) and Th2 cell-mediated inflammation contribute to its pathogenesis. Although it has been suggested that androgen and estrogen were involved in development of asthma, the underlying mechanisms remained largely unclear. Studies have demonstrated that Runx3 could promote naive CD4+ T cells to differentiate into Th1 cells. Hence, our study aimed to explore the potential regulatory mechanism of androgen and estrogen on asthma via modulating Runx3. Methods: First, clinical assessments and pulmonary function tests were conducted on 35 asthma patients and 24 healthy controls. The concentrations of androgen, estrogen, and androgen estrogen ratios were assessed in peripheral blood samples of asthma patients and healthy controls. Then, a murine asthma model was established to explore the effects of estrogen and androgen (alone or in combination) on asthma. Third, an in vitro assay was used to explore the mechanism of combination of androgen and estrogen in asthma. Results: We observed decreased androgen and increased estrogen levels in asthma patients compared with healthy controls. In mice with experimental asthma, there were increased serum concentrations of estrogen and decreased serum concentrations of androgen, intervention with combination of androgen and estrogen alleviated airway inflammations, increased Runx3 expressions and elevated Th1 differentiation. In CD4+ T cells co-cultured with bronchial epithelial cells (BECs), treatment with androgen plus estrogen combination promoted Th1 differentiation, which was mitigated by Runx3 knockdown in BECs and enhanced by Runx3 overexpression. Conclusion: These findings suggest that androgen estrogen combination modulate the Th1/Th2 balance via regulating the expression of Runx3 in BECs, thereby providing experimental evidence supporting androgen and estrogen combination as a novel therapy for asthma. © The author(s). LA - English DB - MTMT ER - TY - JOUR AU - Chen, Wenjie AU - Liu, Jinghua AU - Shi, Yuchen TI - Development of a Novel Nomogram to Predict Major Adverse Cardiac Events in Patients with Chronic Total Occlusion JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 6 SP - 1091 EP - 1102 PG - 12 SN - 1449-1907 DO - 10.7150/ijms.94644 UR - https://m2.mtmt.hu/api/publication/35021731 ID - 35021731 LA - English DB - MTMT ER - TY - JOUR AU - Nguyen, Uyen Thi Nhat AU - Hsieh, Han-Yun AU - Chin, Tzu-Yun AU - Wu, Guani AU - Lin, Yen Po AU - Lee, Ching-Yi AU - Hsu, Yi-Chiung AU - Fan, Yu-Jui TI - Evaluation of Pm2.5 Influence on Human Lung Cancer Cells Using a Microfluidic Platform JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 6 SP - 1117 EP - 1128 PG - 12 SN - 1449-1907 DO - 10.7150/ijms.94803 UR - https://m2.mtmt.hu/api/publication/34982831 ID - 34982831 LA - English DB - MTMT ER - TY - JOUR AU - Xu, Da -Ming AU - Chen, Ling-Xiao AU - Zhuang, Xiao-Yu AU - Han, Hui AU - Mo, Miao TI - The Role of JAK-STAT-SOCS1 Axis in Tumorigenesis, Malignant Progression and Lymphatic Metastasis of Penile Cancer JF - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES J2 - INT J MED SCI VL - 21 PY - 2024 IS - 6 SP - 1176 EP - 1186 PG - 11 SN - 1449-1907 DO - 10.7150/ijms.95490 UR - https://m2.mtmt.hu/api/publication/34975021 ID - 34975021 N1 - State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China Department of Urology, Xiangya Hospital, Central South University, Changsha, 410008, China Department of Anesthesiology, Second Affiliated Hospital of Shantou University Medical College, Shantou, 515041, China Export Date: 11 September 2024 Correspondence Address: Han, H.; State Key Laboratory of Oncology in South China, China; email: hanhui@sysucc.org.cn Correspondence Address: Mo, M.; Department of Urology, China; email: momiao@hotmail.com Chemicals/CAS: atezolizumab, 1380723-44-3; ipilimumab, 477202-00-9; Janus kinase, 161384-16-3; nivolumab, 946414-94-4; pembrolizumab, 1374853-91-4; SOCS1 protein, human; STAT4 protein, human LA - English DB - MTMT ER -