@article{MTMT:34649575,
	title = {Effect of dietary intervention with Capsicum annuum extract on growth performance, physiological status, innate immune response, and related gene expression in Nile tilapia},
	url = {https://m2.mtmt.hu/api/publication/34649575},
	author = {Ibrahim, Rowida E. and Rhouma, Nasreddin R. and Elbealy, Mohamed A. and Abdelwarith, Abdelwahab A. and Younis, Elsayed M. and Khalil, Samah S. and Khamis, Tarek and Mansour, Abdallah Tageldein and Davies, Simon J. and El-Murr, Abdelhakeem and Rahman, Afaf N. Abdel},
	doi = {10.1016/j.cbpb.2023.110914},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {270},
	unique-id = {34649575},
	issn = {1096-4959},
	keywords = {GROWTH; Oreochromis niloticus; DIETARY SUPPLEMENTATION; Transporter proteins; immune cytokines; Red pepper alcoholic extract},
	year = {2024},
	eissn = {1879-1107}
}

@article{MTMT:34491256,
	title = {Identification of a cryptic functional apolipophorin-III domain within the Prominin-1 gene of Litopenaeus vannamei},
	url = {https://m2.mtmt.hu/api/publication/34491256},
	author = {Hoyos-Gonzalez, N. and Ochoa-Leyva, A. and Benitez-Cardoza, C.G. and Brieba, L.G. and Lukaszewicz, G. and Trasviña-Arenas, C.H. and Sotelo-Mundo, R.R.},
	doi = {10.1016/j.cbpb.2023.110928},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {270},
	unique-id = {34491256},
	issn = {1096-4959},
	year = {2024},
	eissn = {1879-1107}
}

@article{MTMT:34363877,
	title = {Short communication: ROS production and mitochondrial membrane potential in hemocytes of marine bivalves, Mytilus galloprovincialis and Magallana gigas, under hypoosmotic stress},
	url = {https://m2.mtmt.hu/api/publication/34363877},
	author = {Kladchenko, E. S. and Tkachuk, A. A. and Podolskaya, M. S. and Andreyeva, A. Yu.},
	doi = {10.1016/j.cbpb.2023.110901},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {269},
	unique-id = {34363877},
	issn = {1096-4959},
	abstract = {Bivalve mollusks that inhabit low-depth coastal and estuarine areas frequently experience osmotic stress that may be also associated with alterations of antioxidant enzyme activities and markers of oxidative stress. Mitochondria are a major source of reactive oxygen species (ROS) in eucaryotic cells. Overpoduction of ROS induces oxidative stress leading to a damage of intracellular compartments and cell death. In euryhaline bivalves, information concerning cellular ROS production upon osmotic stress and changes in mitochondrial membrane potential is scarce. The present study investigates osmotic stability and hemocytes` regulatory volume decrease (RVD) of Mediterranean mussel (Mytilus galloprovincialis) and the Pacific oyster (Magallana gigas). We also studied dynamic changes in intracellular ROS levels and mitochondrial membrane potential in hemocytes undergoing the RVD response following hypoosmotic swelling. Our data revealed that osmotic stability of mussel and oyster hemocytes did not significantly differ. Loss of environmental osmolarity from 460.0 +/- 2.0 mOsm l- 1 to 216.0 +/- 4.0 mOsm l- 1 resulted in an increase of hemocyte volume by 60% of the initial cellular volume in mussels and by 28% in oysters. After rapid hypoosmotic swelling hemocytes of both species demonstrated the RVD response. At the end of 60 min exposure to hypoosmotic environment, hemocyte volume significantly decreased in both species by 10-12% compared to the maximal hemocyte volume. Hypoosmotic shock induced an increase of mitochondrial membrane potential in hemocytes of mussels and oysters. In mussels, increased mitochondrial membrane potential was accompanied with decreased ROS levels in hemocytes, whereas oyster hemocytes showed enhanced ROS},
	keywords = {REGULATORY VOLUME DECREASE; Reactive oxygen species; mitochondrial membrane potential; Hemocyte; Bivalves; Oxidative stress; Osmotic stability},
	year = {2024},
	eissn = {1879-1107}
}

@article{MTMT:34247318,
	title = {Induced swimming modified the antioxidant status of gilthead seabream (Sparus aurata)},
	url = {https://m2.mtmt.hu/api/publication/34247318},
	author = {Espirito-Santo, Carlos and Alburquerque, Carmen and Guardiola, Francisco A. and Ozorio, Rodrigo O. A. and Magnoni, Leonardo J.},
	doi = {10.1016/j.cbpb.2023.110893},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {269},
	unique-id = {34247318},
	issn = {1096-4959},
	abstract = {ySwimming has relevant physiological changes in farmed fish, although the potential link between swimming and oxidative stress remains poorly studied. We investigated the effects of different medium-term moderate swimming conditions for 6 h on the antioxidant status of gilthead seabream (Sparus aurata), analyzing the activity of enzymes related to oxidative stress in the liver and skeletal red and white muscle. Forty fish were induced to swim individually with the following conditions: steady low (SL, 0.8 body length (BL)center dot s(-1)), steady high (SH, 2.3 BL center dot s(-1)), oscillating low (OL, 0.2-0.8 BL center dot s(-1)) and oscillating high (OH, 0.8-2.3 BL center dot s(-1)) velocities, and a non-exercised group with minimal water flow (MF, < 0.1 BL center dot s(-1)). All swimming conditions resulted in lower activities of superoxide dismutase (SOD), glutathione reductase (GR), and glutathione-S-transferase (GST) in the liver compared to the MF group, while steady swimming (SL and SH) led to higher reduced glutathione/oxidized glutathione ratio (GSH/GSSG) compared to the MF condition. Swimming also differently modulated the antioxidant enzyme activities in red and white muscles. The OH condition increased lipid peroxidation (LPO), catalase (CAT) and glutathione peroxidase (GPx) activities in the red muscle, decreasing the GSH/GSSG ratio, whereas the SL condition led to increased GSH. Oscillating swimming conditions (OL and OH) led to lower CAT activity in the white muscle, although GPx activity was increased. The GSH/GSSG ratio in white muscle was increased in all swimming conditions. Liver and skeletal muscle antioxidant status was modulated by exercise, highlighting the importance of adequate swimming conditions to minimize oxidative stress in gilthead seabream.},
	keywords = {swimming; Exercise; Oxidative stress; Glutathione system; Gilthead seabream ( Sparus aurata )},
	year = {2024},
	eissn = {1879-1107}
}

@article{MTMT:34237387,
	title = {Phosphofructokinase family genes in grass carp: Molecular identification and tissue-specific expression in response to glucose, insulin and glucagon},
	url = {https://m2.mtmt.hu/api/publication/34237387},
	author = {Zhang, Yingxin and Qin, Chaobin and Wang, Junli and Yang, Liping and Yan, Xiao and Zhi, Shaoyang and Nie, Guoxing},
	doi = {10.1016/j.cbpb.2023.110898},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {269},
	unique-id = {34237387},
	issn = {1096-4959},
	abstract = {It is widely acknowledged that glucose serves as the primary energy source for organisms. However, fish exhibit persistent postprandial hyperglycemia and are thought to have low glucose tolerance. Glycolysis serves as the ubiquitous pathway for glucose catabolism, with phosphofructokinase (PFK) acting as a crucial rate-limiting enzyme in this process and playing an indispensable role. PFK is classified into three isoforms based on their major expression sites, i.e., PFKM (skeletal muscle type), PFKL (liver type) and PFKP (platelet type). In this study, grass carp (Ctenopharyngodon idella) was used as animal model and the open reading frame (ORF) sequences of six PFK genetic isoforms of grass carp were cloned. Real-time PCR was used to detect its tissue distribution, and expression changes in oral glucose tolerance test (OGTT), insulin and glucagon injection experiments. The results showed that the ORF of pfkla, pfklb, pfkma, pfkmb, pfkpa and pfkpb genes was 2343, 2340, 2355, 2331, 2364 and 2349 bp in length, respectively. The results of tissue distribution showed that pfkla and pfklb, homologous to mammalian pfkl, exhibited low expression levels in the liver of grass carp, but were expressed at the highest level in the brain. Muscle-type pfkma and pfkmb mRNA were found to be highly expressed in both red and white muscle, with pfkmb also exhibiting high expression levels in the heart, while platelet type pfkpa and pfkpb showed high mRNA abundances in the brain and heart. Oral glucose administration stimulated pfkma and pfkmb mRNA expression in the red muscle, and up-regulated pfklb mRNA levels in the liver at 3 h post treatment, but it suppressed liver-type and platelet-type PFK genes expression in the brain. The expression of pfkmb in white muscle and pfkmb and pfkpb in heart were promoted by insulin, whereas the expression of pfkla and pfkpb in the brain, pfkma and pfkmb in the red muscle, pfkma in the white muscle, and pfklb in the liver was suppressed by insulin. As for glucagon, it inhibited pfkma and pfkmb mRNA expression in the red muscle, as well as pfklb in the liver, but it up-regulated PFK genes expression in most tissues detected, such as brain (pfklb, pfkpa and pfkpb), white muscle (pfkma and pfkmb), liver (pfkla) and heart (pfkmb and pfkpb). Our results suggest that PFK family genes have different or even opposite expression patterns in response to glucose, insulin and glucagon stimulation in various tissues of grass carp, which may contribute to glucose intolerance in fish.},
	keywords = {TISSUE DISTRIBUTION; GLUCAGON; INSULIN; fish; glucose metabolism; phosphofructokinase},
	year = {2024},
	eissn = {1879-1107}
}

@article{MTMT:34321010,
	title = {Identification and ligand binding of a chemosensory protein from sea louse Caligus rogercresseyi (Crustacea: Copepoda)},
	url = {https://m2.mtmt.hu/api/publication/34321010},
	author = {Pino, Jorge and Godoy, Ricardo and Venthur, Herbert and Larama, Giovanni and Quiroz, Andres and Mutis, Ana},
	doi = {10.1016/j.cbpb.2023.110830},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {265},
	unique-id = {34321010},
	issn = {1096-4959},
	abstract = {Caligus rogercresseyi is an ectoparasitic copepod that negatively affects the salmon farming industry, causing economic losses. To use phytochemicals as feed additives, or other chemicals that could elicit behavioral re-sponses in C. rogercresseyi, the chemosensory recognition process is crucial. Therefore, to establish how C. rogercresseyi recognizes glucosinolates and their derivates isothiocyanates, a chemosensory protein (CSP) described as specific carrier of these chemicals in sea louse (CrogCSP) was identified in this study. The recom-binant CSP and its selectivity against different chemical compounds was tested by fluorescence binding assays. Phylogenetic analysis revealed a close relationship among CrogCSP and other reported CSPs. Our results indicate that phenyl isothiocyanate and isophorone exhibited dissociation constants of 4.17 and 4.28 mu M of Ki, respec-tively, indicating affinity over other chemicals, such as fatty acids and sinigrin. Structural findings suggest a unique binding site capable of accept several types of chemicals, similar to what has been reported for crys-tallized insect CSPs. Finally, this study lays the foundation for a deeper understanding of CSPs in crustaceans and especially in C. rogercresseyi. Likewise, the identification of chemosensory proteins could serve as the first step towards novel semiochemicals discovery to being applied in the sea louse controlling.},
	keywords = {olfaction; glucosinolates; Reverse chemical ecology; Chemosensory proteins (CSPs); Sea louse},
	year = {2023},
	eissn = {1879-1107},
	orcid-numbers = {Larama, Giovanni/0000-0002-9658-7752}
}

@article{MTMT:34247285,
	title = {Morpho-functional changes of lungfish Protopterus dolloi skin in the shift from freshwater to aestivating conditions},
	url = {https://m2.mtmt.hu/api/publication/34247285},
	author = {Amelio, Daniela and Garofalo, Filippo},
	doi = {10.1016/j.cbpb.2023.110846},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {266},
	unique-id = {34247285},
	issn = {1096-4959},
	abstract = {African dipnoi (Protopterus sp.) are obligate air-breathing fish that, during dry season, may experience a period of dormancy named aestivation. Aestivation is characterized by complete reliance on pulmonary breathing, general decrease of metabolism and down-regulation of respiratory and cardiovascular functions.To date, little is known about morpho-functional rearrangements induced by aestivation in the skin of African lungfishes. Our study aims to identify, in the skin of P. dolloi, structural modifications and stress-induced molecules in response to short-term (6 days) and long-term (40 days) aestivation.Light microscopy showed that short-term aestivation induces major reorganization, with narrowing of epidermal layers and decrease of mucous cells; prolonged aestivation is characterized by regenerative processes and re-thickening of epidermal layers. Immunofluorescence reveals that aestivation correlates with an increased oxidative stress and changes of Heat Shock Proteins expression, suggesting a protective role for these chaperons.Our findings revealed that lungfish skin undergoes remarkable morphological and biochemical readjustments in response to stressful conditions associated with aestivation.},
	keywords = {SKIN; MORPHOLOGY; Thermal stress; Aestivation; lungfish},
	year = {2023},
	eissn = {1879-1107}
}

@article{MTMT:34075375,
	title = {Potential role of miR-8159-x in heat stress response in rainbow trout (Oncorhynchus mykiss)},
	url = {https://m2.mtmt.hu/api/publication/34075375},
	author = {Zhao, Guiyan and Liu, Zhe and Quan, Jinqiang and Sun, Jun and Li, Lanlan and Lu, Junhao},
	doi = {10.1016/j.cbpb.2023.110877},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {268},
	unique-id = {34075375},
	issn = {1096-4959},
	year = {2023},
	eissn = {1879-1107}
}

@article{MTMT:33431274,
	title = {DNA double-strand break repair machinery in Penaeid crustaceans: A focus on the Non-Homologous End-Joining pathway},
	url = {https://m2.mtmt.hu/api/publication/33431274},
	author = {Buendia-Padilla, Monica and Garcia-Carreno, Fernando and Velazquez-Lizarraga, Adrian E. and Rojo-Arreola, Liliana},
	doi = {10.1016/j.cbpb.2022.110803},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {264},
	unique-id = {33431274},
	issn = {1096-4959},
	abstract = {DNA double-strand breaks (DSBs) are repaired through three major pathways: Non-Homologous End-Joining (NHEJ), Microhomology-Mediated End-Joining (MMEJ), and Homology-Directed Repair (HDR), each requiring a specific set of diverse proteins. Such pathways and their proteins have been studied in model organisms, including arthropods; however, DSB repair pathways are scarcely described in Crustacea, a taxon that includes the commercially valuable penaeid shrimps (Crustacea: Decapoda: Penaeidae). In this work, transcriptome and proteome databases of Penaeus vannamei and other Crustacea species were scrutinized for each protein of the NHEJ pathway. The structural and functional attributes of such proteins in penaeids were determined using bioinformatics. Additionally, the expression of the NHEJ-related Ku70, Ku80, DNA-PKcs, DNA ligase 4 (Lig4), and HDR-and MMEJ-related protein transcripts were assessed in P. vannamei gills, midgut gland, hemocytes, and muscle by RT-PCR. DSB repair protein transcripts were found expressed in the four assayed tissues, particularly in the gills and midgut gland. Among DSB repair proteins, all the analyzed transcripts of proteins related to the NHEJ pathway were present in gills. To the best of our knowledge, this is the first report on the expression of DSB repair proteins in Decapoda. Together, proteomic, transcriptomic, and expression data suggest the functionality of NHEJ, HDR, and MMEJ pathways in P. vannamei and other decapods. The information presented here con-tributes to understanding the response to DSB breaks in shrimps, describing possible outcomes in oxidative stress studies and also in the designing of gene editing strategies, which have not been developed in Penaeidae.},
	keywords = {Crustacea; bioinformatics; Gene editing; DSB repair; Penaeid},
	year = {2023},
	eissn = {1879-1107}
}

@article{MTMT:33351896,
	title = {Liver vs. spleen: Time course of organ-dependent immune gene expression in an LPS-stimulated toad (Rhinella diptycha)},
	url = {https://m2.mtmt.hu/api/publication/33351896},
	author = {Floreste, Felipe R. and Titon Jr, Braz and Titon, Stefanny C. M. and Muxel, Sandra M. and Figueiredo, Aymam C. de and Gomes, Fernando R. and Assis, Vania R.},
	doi = {10.1016/j.cbpb.2022.110784},
	journal-iso = {COMP BIOCHEM PHYS B},
	journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY},
	volume = {263},
	unique-id = {33351896},
	issn = {1096-4959},
	abstract = {The inflammatory response comprises highly orchestrated events that are conserved amongst vertebrate groups. Hepatic and splenic cytokines are major mediators of the systemic inflammatory processes. However, the liver is still neglected as an immune organ in amphibians. This study reports organ-dependent gene expression using an anuran model. We tracked mRNA levels of immune proteins [C1s (subcomponent S of the complement protein 1), IFN-gamma, IL-beta, IL-6, and IL-10] at four time-points (1 h, 3 h, 6 h, and 18 h post-injection) in spleens and livers of intraperitoneal LPS-challenged (2 mg/kg) adult male toads (Rhinella diptycha) using independent samples. We found acute C1s up-regulation in the liver 1 h post-injection, with no treatment effect in the spleen. The LPS injection did not show any effect in splenic IFN-gamma gene expression while eliciting only a marginal effect in the hepatic tissue. IL-beta was up-regulated in both organs, with the liver initially displaying early expression (1 h and 3 h) and the spleen taking over late expression (18 h). Both organs exhibited similar patterns for IL-6, with early up-regulation (1 h and 3 h) and late peak (18 h). Although IL-10 was early detected and up-regulated only in the liver, both organs showed up-regulation in 6 h and 18 h post-injection. Our results show an exclusive hepatic prominence in complement protein expression during the acute-phase response. Furthermore, hepatic pro -inflammatory cytokine expression was more pronounced in earliest time-points, while the spleen offers a slower and more consistent response overall. Our data provide an organ-integrative outlook into the initial hours of the inflammation in amphibians, confirming the liver's pivotal role as a regulator in the acute-phase of the inflammatory response in amphibians.},
	keywords = {Cytokines; Complement system; Inflammatory response; AMPHIBIANS; MRNA levels; In vivo immune-challenge},
	year = {2023},
	eissn = {1879-1107}
}