@article{MTMT:34857362,
	title = {Remembering Jan Amesz (1934-2001): a great gentleman, a major discoverer, and an internationally renowned biophysicist of both oxygenic and anoxygenic photosynthesisa},
	url = {https://m2.mtmt.hu/api/publication/34857362},
	author = {Govindjee, Govindjee and Amesz, Bas and Garab, Győző and Stirbet, Alexandrina},
	doi = {10.1007/s11120-024-01102-9},
	journal-iso = {PHOTOSYNTH RES},
	journal = {PHOTOSYNTHESIS RESEARCH},
	unique-id = {34857362},
	issn = {0166-8595},
	abstract = {We present here the research contributions of Jan Amesz (1934-2001) on deciphering the details of the early physico-chemical steps in oxygenic photosynthesis in plants, algae and cyanobacteria, as well as in anoxygenic photosynthesis in purple, green, and heliobacteria. His research included light absorption and the mechanism of excitation energy transfer, primary photochemistry, and electron transfer steps until the reduction of pyridine nucleotides. Among his many discoveries, we emphasize his 1961 proof, with L. N. M. Duysens, of the "series scheme" of oxygenic photosynthesis, through antagonistic effects of Light I and II on the redox state of cytochrome f. Further, we highlight the following research on oxygenic photosynthesis: the experimental direct proof that plastoquinone and plastocyanin function at their respective places in the Z-scheme. In addition, Amesz's major contributions were in unraveling the mechanism of excitation energy transfer and electron transport steps in anoxygenic photosynthetic bacteria (purple, green and heliobacteria). Before we present his research, focusing on his key discoveries, we provide a glimpse of his personal life. We end this Tribute with reminiscences from three of his former doctoral students (Sigi Neerken; Hjalmar Pernentier, and Frank Kleinherenbrink) and from several scientists (Suleyman Allakhverdiev; Robert Blankenship; Richard Cogdell) including two of the authors (G. Garab and A. Stirbet) of this Tribute.},
	keywords = {ENERGY-TRANSFER; BACTERIOCHLOROPHYLL; electron transfer; Reaction center; primary photochemistry; CHLOROPHYLL; PLASTOCYANIN; Green sulfur bacteria; PLASTOQUINONE; excitation energy transfer; anoxygenic photosynthesis; Primary charge separation; Cytochrome f; Heliobacteria; Carnegie Institute of Washington; Chromatium sp.; Louis (Lou) NM Duysens; Rhodopseudomonas sp.; Rhodospirillum sp; Two-electron gate; Winkler Prins prize; PHOSPHOPYRIDINE NUCLEOTIDE REDUCTION; FLUORESCENCE EMISSION-SPECTRA; REACTION-CENTER COMPLEXES; 2 PHOTOCHEMICAL SYSTEMS; FMO ANTENNA COMPLEXES; CENTER CORE COMPLEXES},
	year = {2024},
	eissn = {1573-5079}
}

@article{MTMT:34850122,
	title = {Role of isotropic lipid phase in the fusion of photosystem II membranes},
	url = {https://m2.mtmt.hu/api/publication/34850122},
	author = {Böde, Kinga and Javornik, Uros and Dlouhy, Ondrej and Zsíros, Ottó and Biswas, Avratanu and Racskóné Domonkos, Ildikó and Sket, Primoz and Karlicky, Vaclav and Ughy, Bettina and Lambrev, Petar and Spunda, Vladimir and Plavec, Janez and Garab, Győző},
	doi = {10.1007/s11120-024-01097-3},
	journal-iso = {PHOTOSYNTH RES},
	journal = {PHOTOSYNTHESIS RESEARCH},
	unique-id = {34850122},
	issn = {0166-8595},
	abstract = {It has been thoroughly documented, by using P-31-NMR spectroscopy, that plant thylakoid membranes (TMs), in addition to the bilayer (or lamellar, L) phase, contain at least two isotropic (I) lipid phases and an inverted hexagonal (H-II) phase. However, our knowledge concerning the structural and functional roles of the non-bilayer phases is still rudimentary. The objective of the present study is to elucidate the origin of I phases which have been hypothesized to arise, in part, from the fusion of TMs (Garab et al. 2022 Progr Lipid Res 101,163). We take advantage of the selectivity of wheat germ lipase (WGL) in eliminating the I phases of TMs (Dlouh & yacute; et al. 2022 Cells 11: 2681), and the tendency of the so-called BBY particles, stacked photosystem II (PSII) enriched membrane pairs of 300-500 nm in diameter, to form large laterally fused sheets (Dunahay et al. 1984 BBA 764: 179). Our 31P-NMR spectroscopy data show that BBY membranes contain L and I phases. Similar to TMs, WGL selectively eliminated the I phases, which at the same time exerted no effect on the molecular organization and functional activity of PSII membranes. As revealed by sucrose-density centrifugation, magnetic linear dichroism spectroscopy and scanning electron microscopy, WGL disassembled the large laterally fused sheets. These data provide direct experimental evidence on the involvement of I phase(s) in the fusion of stacked PSII membrane pairs, and strongly suggest the role of non-bilayer lipids in the self-assembly of the TM system.},
	keywords = {MODEL; DYNAMICS; HIGHER-PLANTS; Membrane Fusion; CHLOROPHYLL-A; THYLAKOID MEMBRANE; A/B PROTEIN COMPLEX; NONBILAYER LIPIDS; violaxanthin de-epoxidase; linear dichroism spectroscopy; non-bilayer lipids; P-31-NMR spectroscopy; BBY membrane; XANTHOPHYLL CYCLE ENZYME},
	year = {2024},
	eissn = {1573-5079}
}

@article{MTMT:34797803,
	title = {Effects of lipids on the rate-limiting steps in the dark-to-light transition of Photosystem II core complex of Thermostichus vulcanus},
	url = {https://m2.mtmt.hu/api/publication/34797803},
	author = {Magyar, Melinda and Akhtar, Parveen and Sipka, Gábor and Racskóné Domonkos, Ildikó and Han, W. and Li, X. and Han, G. and Shen, J.-R. and Lambrev, Petar and Garab, Győző},
	doi = {10.3389/fpls.2024.1381040},
	journal-iso = {FRONT PLANT SCI},
	journal = {FRONTIERS IN PLANT SCIENCE},
	volume = {15},
	unique-id = {34797803},
	issn = {1664-462X},
	abstract = {In our earlier works, we have shown that the rate-limiting steps, associated with the dark-to-light transition of Photosystem II (PSII), reflecting the photochemical activity and structural dynamics of the reaction center complex, depend largely on the lipidic environment of the protein matrix. Using chlorophyll-a fluorescence transients (ChlF) elicited by single-turnover saturating flashes, it was shown that the half-waiting time (Δτ1/2) between consecutive excitations, at which 50% of the fluorescence increment was reached, was considerably larger in isolated PSII complexes of Thermostichus (T.) vulcanus than in the native thylakoid membrane (TM). Further, it was shown that the addition of a TM lipid extract shortened Δτ1/2 of isolated PSII, indicating that at least a fraction of the ‘missing’ lipid molecules, replaced by detergent molecules, caused the elongation of Δτ1/2. Here, we performed systematic experiments to obtain information on the nature of TM lipids that are capable of decreasing Δτ1/2. Our data show that while all lipid species shorten Δτ1/2, the negatively charged lipid phosphatidylglycerol appears to be the most efficient species – suggesting its prominent role in determining the structural dynamics of PSII reaction center. Copyright © 2024 Magyar, Akhtar, Sipka, Domonkos, Han, Li, Han, Shen, Lambrev and Garab.},
	keywords = {STRUCTURAL DYNAMICS; CHLOROPHYLL-A FLUORESCENCE; Waiting time; Thylakoid lipids; Rate-limiting step; core complex of photosystem II},
	year = {2024},
	eissn = {1664-462X},
	orcid-numbers = {Magyar, Melinda/0000-0002-1144-4657; Akhtar, Parveen/0000-0002-3264-7154; Sipka, Gábor/0000-0002-8553-4890}
}

@article{MTMT:34764951,
	title = {Comparative transcriptome study highlights the versatility of nitrogen metabolism in Chlamydomonas},
	url = {https://m2.mtmt.hu/api/publication/34764951},
	author = {Rani, Vaishali and Shetty, Prateek and Maróti, Gergely},
	doi = {10.1016/j.algal.2024.103458},
	journal-iso = {ALGAL RES},
	journal = {ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS},
	volume = {79},
	unique-id = {34764951},
	issn = {2211-9264},
	abstract = {Nitrogen is an essential macronutrient and nitrate is one of the main forms of this macronutrient available for plants and microbes. Nitrate is not only the substrate for the nitrate assimilation pathway, but also a crucial signal for the regulation of numerous metabolic, developmental, and cellular differentiation processes. In the present study, two species of the Chlamydomonas genus, Chlamydomonas reinhardtii cc124 and Chlamydomonas sp. MACC-216 were used to investigate the versatility of nitrate metabolism in green microalgae. Quantification of nitrate removal efficiency showed that Chlamydomonas sp. MACC-216 strongly outperforms C. reinhardtii cc124. Transcriptional changes occurring under nitrate-replete and nitrate-deplete conditions were specifically investigated in the selected species of Chlamydomonas. Whole transcriptome analysis revealed that the genes playing a role in nitrate assimilation did not show differential expression in C. reinhardtii cc124 under changing nitrate conditions (only 45 genes exhibited differential regulation), while in Chlamydomonas sp. MACC-216 a large set of genes (3143) showed altered expression. Furthermore, genes responsible for urea metabolism, like DUR3A gene corresponding to urea transport, were found to be upregulated in Chlamydomonas sp. MACC-216 under nitrate-deplete condition, while the same gene showed elevated expression level in C. reinhardtii cc124 under nitrate-replete condition. The present study indicated the diverseness of nitrate metabolism among species within the Chlamydomonas genus.},
	keywords = {Chlamydomonas; transcriptome analysis; urea metabolism; Nitrate-deplete; Nitrate-replete},
	year = {2024},
	eissn = {2211-9264},
	pages = {1-13},
	orcid-numbers = {Maróti, Gergely/0000-0002-3705-0461}
}

@article{MTMT:34730183,
	title = {Apis mellifera filamentous virus from a honey bee gut microbiome survey in Hungary},
	url = {https://m2.mtmt.hu/api/publication/34730183},
	author = {Papp, Márton and Tóth, Adrienn Gréta and Békési, László and Farkas, Róbert László and Makrai, László and Maróti, Gergely and Solymosi, Norbert},
	doi = {10.1038/s41598-024-56320-x},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {14},
	unique-id = {34730183},
	issn = {2045-2322},
	abstract = {In Hungary, as part of a nationwide, climatically balanced survey for a next-generation sequencing-based study of the honey bee (Apis mellifera) gut microbiome, repeated sampling was carried out during the honey production season (March and May 2019). Among other findings, the presence of Apis mellifera filamentous virus (AmFV) was detected in all samples, some at very high levels. AmFV-derived reads were more abundant in the March samples than in the May samples. In March, a higher abundance of AmFV-originated reads was identified in samples collected from warmer areas compared to those collected from cooler areas. A lower proportion of AmFV-derived reads were identified in samples collected in March from the wetter areas than those collected from the drier areas. AmFV-read abundance in samples collected in May showed no significant differences between groups based on either environmental temperature or precipitation. The AmFV abundance correlated negatively with Bartonella apihabitans, Bartonella choladocola, and positively with Frischella perrara, Gilliamella apicola, Gilliamella sp. ESL0443, Lactobacillus apis, Lactobacillus kullabergensis, Lactobacillus sp. IBH004. De novo metagenome assembly of four samples resulted in almost the complete AmFV genome. According to phylogenetic analysis based on DNA polymerase, the Hungarian strains are closest to the strain CH-05 isolated in Switzerland.},
	year = {2024},
	eissn = {2045-2322},
	pages = {1-8},
	orcid-numbers = {Papp, Márton/0000-0003-4975-253X; Maróti, Gergely/0000-0002-3705-0461; Solymosi, Norbert/0000-0003-1783-2041}
}

@article{MTMT:34721677,
	title = {Mitochondrial complex I subunit NDUFS8.2 modulates responses to stresses associated with reduced water availability},
	url = {https://m2.mtmt.hu/api/publication/34721677},
	author = {Zsigmond, Laura and Juhász-Erdélyi, Annabella and Valkai, Ildikó and Aleksza, Dávid and Rigó, Gábor and Kant, Kamal and Szepesi, Ágnes and Fiorani, Fabio and Körber, Niklas and Kovács, László and Szabados, László},
	doi = {10.1016/j.plaphy.2024.108466},
	journal-iso = {PLANT PHYSIOL BIOCH (PPB)},
	journal = {PLANT PHYSIOLOGY AND BIOCHEMISTRY},
	volume = {208},
	unique-id = {34721677},
	issn = {0981-9428},
	year = {2024},
	eissn = {1873-2690},
	orcid-numbers = {Zsigmond, Laura/0000-0002-1388-1762; Szepesi, Ágnes/0000-0003-3043-4857}
}

@article{MTMT:34721641,
	title = {Mutation in Arabidopsis mitochondrial Pentatricopeptide repeat 40 gene affects tolerance to water deficit},
	url = {https://m2.mtmt.hu/api/publication/34721641},
	author = {Kant, Kamal and Rigó, Gábor and Faragó, Dóra and Benyó, Dániel and Tengölics, Roland and Szabados, László and Zsigmond, Laura},
	doi = {10.1007/s00425-024-04354-w},
	journal-iso = {PLANTA},
	journal = {PLANTA},
	volume = {259},
	unique-id = {34721641},
	issn = {0032-0935},
	year = {2024},
	eissn = {1432-2048},
	orcid-numbers = {Benyó, Dániel/0000-0002-4537-2866; Zsigmond, Laura/0000-0002-1388-1762}
}

@article{MTMT:34598013,
	title = {Accumulation of reactive carbonyl species in roots as the primary cause of salt stress-induced growth retardation of Arabidopsis thaliana},
	url = {https://m2.mtmt.hu/api/publication/34598013},
	author = {Sultana, Most. Sharmin and Sakurai, Chisato and Biswas, Md. Sanaullah and Szabados, László and Mano, Jun'ichi},
	doi = {10.1111/ppl.14198},
	journal-iso = {PHYSIOL PLANTARUM},
	journal = {PHYSIOLOGIA PLANTARUM},
	volume = {176},
	unique-id = {34598013},
	issn = {0031-9317},
	abstract = {Salt stress on plants induces an increase in reactive oxygen species (ROS), which then leads to the formation of reactive carbonyl species (RCS) such as acrolein and 4-hydroxy-(E)-2-nonenal (HNE), potent cytotoxins generated from lipid peroxides. We recently showed that salt-stress treatment of Arabidopsis thaliana plants increased RCS levels, and exogenously added RCS-scavenging chemicals alleviated the stress symptoms, indicating that RCS were responsible for the tissue damage in salt-stressed plants. To obtain deeper insights into the role of RCS in stressed plants, we here analyzed changes in the levels of various RCS in the roots and shoots of A. thaliana. NaCl (90 mM) addition to the culture medium as a salt-stress treatment caused growth inhibition and leaf chlorosis. Carbonyl analysis using HPLC revealed that the stress treatment induced a 2-fold increase in the root levels of RCS, including acrolein, HNE and 4-hydroxy-(E)-2-hexenal (HHE). In the shoots, basal levels and stress-induced increases of the RCS were lower than in roots. In the transgenic A. thaliana plants that overexpress the RCS-scavenging enzyme 2-alkenal reductase (AER) cDNA under the beta-estradiol (beta-ED)-responsive promoter, salt stress induced less damage than in the wild-type under beta-ED supplementation. The AER overexpression suppressed the stress-induced increases in HNE, acrolein, HHE and (E)-2-hexenal in roots and in HNE in leaves, but not the ROS increase. These results suggest that the RCS increase in roots was the primary cause of salt-induced damages. Enhancing RCS-scavenging abilities, such as by AER overexpression, could be a new strategy to confer salt-stress tolerance to plants.},
	year = {2024},
	eissn = {1399-3054}
}

@article{MTMT:34575642,
	title = {Structural and Functional Analysis of Groundnut bud necrosis virus (GBNV) Using Computational and Biochemical Approaches},
	url = {https://m2.mtmt.hu/api/publication/34575642},
	author = {Jamwal, Rohit and Devi, Pukhrambam Pushpa and Rani, Vaishali and Rawat, Nitish and Daimei, Guisuibou and Saurav, Gunjan Kumar and Renukadevi, Perumal and Yadav, Karuna and Rajagopal, Raman},
	doi = {10.1007/s12033-024-01046-4},
	journal-iso = {MOL BIOTECHNOL},
	journal = {MOLECULAR BIOTECHNOLOGY},
	unique-id = {34575642},
	issn = {1073-6085},
	abstract = {Groundnut bud necrosis virus (GBNV) belonging to the genus Orthotospovirus is transmitted by its vector Thrips palmi. It is a tri-segmented RNA virus that consists of L, M, and S RNA segments. We analysed the secondary structure features of GBNV proteins through various software and predicted the transmembrane helix, glycosylation, and signal peptidase sites within the GBNV protein sequences (G(N), G(C), N, NSm, and NSs). In glycoprotein sequence, extended strands are predominant (52.87%) whereas the N protein sequence mostly contains alpha helices (47.46%). The random coils are present in movement protein (43.97%) and structural protein (39.41%). We generated the 3D structure of G(N) and N protein using SWISS MODEL software and quality is validated through PROCHECK and PDBsum software. We also expressed the GBNV proteins (G(N), G(C), N, NSm, and NSs) in bacterial expression system. The recombinant proteins were used to raise polyclonal antibodies in mice. Our study will be useful in understanding GBNV protein structures in further detail by analysing the important domains that interact with the thrips proteins. This will further aid us in understanding virus-vector relationship through the application of protein-protein interaction and other immunodiagnostic techniques.},
	keywords = {ANTIBODIES; SPOTTED-WILT-VIRUS; bioinformatics; genome organization; nucleocapsid protein; COMPLETE NUCLEOTIDE-SEQUENCE; Biochemistry & Molecular Biology; 3D model; CELL-TO-CELL; GBNV proteins; MOVEMENT PROTEIN NSM; TUBULE FORMATION; TOSPOVIRUS TSWV; M RNA},
	year = {2024},
	eissn = {1559-0305}
}

@article{MTMT:34575639,
	title = {Energy transfer from phycobilisomes to photosystem I at room temperature},
	url = {https://m2.mtmt.hu/api/publication/34575639},
	author = {Biswas, Avratanu and Akhtar, Parveen and Lambrev, Petar and van, Stokkum Ivo H. M.},
	doi = {10.3389/fpls.2023.1300532},
	journal-iso = {FRONT PLANT SCI},
	journal = {FRONTIERS IN PLANT SCIENCE},
	volume = {14},
	unique-id = {34575639},
	issn = {1664-462X},
	abstract = {The phycobilisomes function as the primary light-harvesting antennae in cyanobacteria and red algae, effectively harvesting and transferring excitation energy to both photosystems. Here we investigate the direct energy transfer route from the phycobilisomes to photosystem I at room temperature in a mutant of the cyanobacterium Synechocystis sp. PCC 6803 that lacks photosystem II. The excitation dynamics are studied by picosecond time-resolved fluorescence measurements in combination with global and target analysis. Global analysis revealed several fast equilibration time scales and a decay of the equilibrated system with a time constant of approximate to 220 ps. From simultaneous target analysis of measurements with two different excitations of 400 nm (chlorophyll a) and 580 nm (phycobilisomes) a transfer rate of 42 ns-1 from the terminal emitter of the phycobilisome to photosystem I was estimated.},
	keywords = {ASSOCIATION; KINETICS; COMPONENTS; CYANOBACTERIA; MUTANT; CHLOROPHYLL; EXCITATION-ENERGY; Phycocyanin; Target analysis; photosystem I (PSI); global analysis (GA); phycobilisomes (PBs); APCD},
	year = {2024},
	eissn = {1664-462X},
	orcid-numbers = {Akhtar, Parveen/0000-0002-3264-7154}
}