TY - JOUR AU - Balog, József Ágoston AU - Horti-Oravecz, Klaudia AU - Kövesdi, Dorottya AU - Bozsik, Anikó AU - Papp, Janos AU - Butz, Henriett AU - Patócs, Attila AU - Szebeni, Gábor AU - Grolmusz, Vince Kornél TI - Peripheral immunophenotyping reveals lymphocyte stimulation in healthy women living with hereditary breast and ovarian cancer syndrome JF - ISCIENCE J2 - ISCIENCE VL - AiP PY - 2024 SP - 109882 SN - 2589-0042 DO - 10.1016/j.isci.2024.109882 UR - https://m2.mtmt.hu/api/publication/34840271 ID - 34840271 LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Enikő AU - Faragó, Anna AU - Bodor, Gergely AU - Gémes, Nikolett AU - Puskás, László AU - Kovács, László AU - Szebeni, Gábor TI - Identification of immune subsets with distinct lectin binding signatures using multi-parameter flow cytometry: correlations with disease activity in systemic lupus erythematosus JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 SN - 1664-3224 DO - 10.3389/fimmu.2024.1380481 UR - https://m2.mtmt.hu/api/publication/34840255 ID - 34840255 LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Zvara, Ágnes AU - Bukovinszki, Vivien AU - Puskás, László AU - Balog, Attila AU - Szebeni, Gábor TI - Comparative single-cell multiplex immunophenotyping of therapy-naive patients with rheumatoid arthritis, systemic sclerosis, and systemic lupus erythematosus shed light on disease-specific composition of the peripheral immune system JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 SN - 1664-3224 DO - 10.3389/fimmu.2024.1376933 UR - https://m2.mtmt.hu/api/publication/34829278 ID - 34829278 LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Fodor, K. AU - Magyar, Lilla Brigitta AU - Lipinszki, Zoltán AU - Hultmark, D. AU - Andó, István TI - Cellular Immunity of Drosophila willistoni Reveals Novel Complexity in Insect Anti-Parasitoid Defense JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 7 SN - 2073-4409 DO - 10.3390/cells13070593 UR - https://m2.mtmt.hu/api/publication/34804520 ID - 34804520 N1 - Innate Immunity Group, Institute of Genetics, HUN-REN Biological Research Centre, Szeged, 6726, Hungary Doctoral School of Biology, University of Szeged, Szeged, 6720, Hungary MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, 6726, Hungary National Laboratory for Biotechnology, Institute of Genetics, HUN-REN Biological Research Centre, Szeged, 6726, Hungary Department of Molecular Biology, Umea University, Umea, 901 87, Sweden Export Date: 22 April 2024 Correspondence Address: Cinege, G.; Innate Immunity Group, Hungary; email: cinege.gyongyi@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Faragó, Anna AU - Zvara, Ágnes AU - Tiszlavicz, László AU - Hunyadi-Gulyás Éva, Csilla AU - Darula, Zsuzsanna AU - Hegedűs, Zoltán AU - Szabó, Enikő AU - Surguta, Sára Eszter AU - Tóvári, József AU - Puskás, László AU - Szebeni, Gábor TI - Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model. JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 21 SN - 1661-6596 DO - 10.3390/ijms25074022 UR - https://m2.mtmt.hu/api/publication/34790193 ID - 34790193 N1 - * Megosztott szerzőség AB - A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies. LA - English DB - MTMT ER - TY - JOUR AU - Vedelek, Viktor AU - Jankovics, Ferenc AU - Zádori, János AU - Sinka, Rita TI - Mitochondrial Differentiation during Spermatogenesis: Lessons from Drosophila melanogaster JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 25 SN - 1661-6596 DO - 10.3390/ijms25073980 UR - https://m2.mtmt.hu/api/publication/34772438 ID - 34772438 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office Funding text: No Statement Available AB - Numerous diseases can arise as a consequence of mitochondrial malfunction. Hence, there is a significant focus on studying the role of mitochondria in cancer, ageing, neurodegenerative diseases, and the field of developmental biology. Mitochondria could exist as discrete organelles in the cell; however, they have the ability to fuse, resulting in the formation of interconnected reticular structures. The dynamic changes between these forms correlate with mitochondrial function and mitochondrial health, and consequently, there is a significant scientific interest in uncovering the specific molecular constituents that govern these transitions. Moreover, the specialized mitochondria display a wide array of variable morphologies in their cristae formations. These inner mitochondrial structures are closely associated with the specific functions performed by the mitochondria. In multiple cases, the presence of mitochondrial dysfunction has been linked to male sterility, as it has been observed to cause a range of abnormal spermatogenesis and sperm phenotypes in different species. This review aims to elucidate the dynamic alterations and functions of mitochondria in germ cell development during the spermatogenesis of Drosophila melanogaster. LA - English DB - MTMT ER - TY - JOUR AU - Chen, Xin AU - Tsvetkov, Andrey S. AU - Shen, Han-Ming AU - Isidoro, Ciro AU - Ktistakis, Nicholas T. AU - Linkermann, Andreas AU - Koopman, Werner J. H. AU - Simon, Hans-Uwe AU - Galluzzi, Lorenzo AU - Luo, Shouqing AU - Xu, Daqian AU - Gu, Wei AU - Peulen, Olivier AU - Cai, Qian AU - Rubinsztein, David C. AU - Chi, Jen-Tsan AU - Zhang, Donna D. AU - Li, Changfeng AU - Toyokuni, Shinya AU - Liu, Jinbao AU - Roh, Jong-Lyel AU - Dai, Enyong AU - Juhász, Gábor AU - Liu, Wei AU - Zhang, Jianhua AU - Yang, Minghua AU - Liu, Jiao AU - Zhu, Ling-Qiang AU - Zou, Weiping AU - Piacentini, Mauro AU - Ding, Wen-Xing AU - Yue, Zhenyu AU - Xie, Yangchun AU - Petersen, Morten AU - Gewirtz, David A. AU - Mandell, Michael A. AU - Chu, Charleen T. AU - Sinha, Debasish AU - Eftekharpour, Eftekhar AU - Zhivotovsky, Boris AU - Besteiro, Sebastien AU - Gabrilovich, Dmitry I. AU - Kim, Do-Hyung AU - Kagan, Valerian E. AU - Bayir, Hulya AU - Chen, Guang-Chao AU - Ayton, Scott AU - Luenemann, Jan D. AU - Komatsu, Masaaki AU - Krautwald, Stefan AU - Loos, Ben AU - Baehrecke, Eric H. AU - Wang, Jiayi AU - Lane, Jon D. AU - Sadoshima, Junichi AU - Yang, Wan Seok AU - Gao, Minghui AU - Munz, Christian AU - Thumm, Michael AU - Kampmann, Martin AU - Yu, Di AU - Lipinski, Marta M. AU - Jones, Jace W. AU - Jiang, Xuejun AU - Zeh, Herbert J. AU - Kang, Rui AU - Klionsky, Daniel J. AU - Kroemer, Guido AU - Tang, Daolin TI - International consensus guidelines for the definition, detection, and interpretation of autophagy-dependent ferroptosis JF - AUTOPHAGY J2 - AUTOPHAGY PY - 2024 PG - 34 SN - 1554-8627 DO - 10.1080/15548627.2024.2319901 UR - https://m2.mtmt.hu/api/publication/34770405 ID - 34770405 N1 - Funding Agency and Grant Number: National Natural Science Foundation of China [82272660]; Plan on Enhancing Scientific Research in GMU [02-410-2302289XM]; National Institutes of Health [NIH] of USA [R01CA160417, R01CA229275, R01CA211070]; NIH [GM131919]; Ligue contre le Cancer [equipe labellisee]; Agence National de la Recherche [ANR]; AMMICa US23/CNRS [UMS3655]; Association pour la recherche sur le cancer [ARC]; Canceropple Ile-de-France; Fondation pour la Recherche Medicale [FRM]; European Joint Programme on Rare Diseases (EJPRD); European Research Council Advanced Investigator Award [101052444]; European Union Horizon 2020 Projects Oncobiome, Prevalung [101095604]; Crimson; Fondation Carrefour; Institut National du Cancer [INCa]; Institut Universitaire de France; LabEx Immuno-Oncology [ANR-18- IDEX-0001]; Mark Foundation; RHU Immunolife; Seerave Foundation; SIRIC Stratified Oncology Cell DNA Repair and Tumor Immune Elimination [SOCRATE]; SIRIC Cancer Research and Personalized Medicine [CARPEM]; IdEx Universite de Paris [ANR-18-IDEX-0001] Funding text: The work was supported by the National Natural Science Foundation of China [82272660]. X.C. was supported by the Plan on Enhancing Scientific Research in GMU [02-410-2302289XM] and the National Natural Science Foundation of China [82272660]. D.T. was supported by grants from the National Institutes of Health [NIH] of USA [R01CA160417, R01CA229275, and R01CA211070]. D.J.K. was supported by NIH grant GM131919. G.K. is supported by the Ligue contre le Cancer [equipe labellisee]; Agence National de la Recherche [ANR] - Projets blancs; AMMICa US23/CNRS UMS3655; Association pour la recherche sur le cancer [ARC]; Canceropple Ile-de-France; Fondation pour la Recherche Medicale [FRM]; a donation by Elior; Equipex Onco- Pheno-Screen; European Joint Programme on Rare Diseases (EJPRD); European Research Council Advanced Investigator Award [ERC-2021- ADG, ICD-Cancer, Grant No. 101052444], European Union Horizon 2020 Projects Oncobiome, Prevalung [grant No. 101095604] and Crimson; Fondation Carrefour; Institut National du Cancer [INCa]; Institut Universitaire de France; LabEx Immuno-Oncology [ANR-18- IDEX-0001]; a Cancer Research ASPIRE Award from the Mark Foundation; the RHU Immunolife; Seerave Foundation; SIRIC Stratified Oncology Cell DNA Repair and Tumor Immune Elimination [SOCRATE]; and SIRIC Cancer Research and Personalized Medicine [CARPEM]. This study contributes to the IdEx Universite de Paris ANR-18-IDEX-0001. AB - Macroautophagy/autophagy is a complex degradation process with a dual role in cell death that is influenced by the cell types that are involved and the stressors they are exposed to. Ferroptosis is an iron-dependent oxidative form of cell death characterized by unrestricted lipid peroxidation in the context of heterogeneous and plastic mechanisms. Recent studies have shed light on the involvement of specific types of autophagy (e.g. ferritinophagy, lipophagy, and clockophagy) in initiating or executing ferroptotic cell death through the selective degradation of anti-injury proteins or organelles. Conversely, other forms of selective autophagy (e.g. reticulophagy and lysophagy) enhance the cellular defense against ferroptotic damage. Dysregulated autophagy-dependent ferroptosis has implications for a diverse range of pathological conditions. This review aims to present an updated definition of autophagy-dependent ferroptosis, discuss influential substrates and receptors, outline experimental methods, and propose guidelines for interpreting the results.Abbreviation: 3-MA:3-methyladenine; 4HNE: 4-hydroxynonenal; ACD: accidentalcell death; ADF: autophagy-dependentferroptosis; ARE: antioxidant response element; BH2:dihydrobiopterin; BH4: tetrahydrobiopterin; BMDMs: bonemarrow-derived macrophages; CMA: chaperone-mediated autophagy; CQ:chloroquine; DAMPs: danger/damage-associated molecular patterns; EMT,epithelial-mesenchymal transition; EPR: electronparamagnetic resonance; ER, endoplasmic reticulum; FRET: Forsterresonance energy transfer; GFP: green fluorescent protein;GSH: glutathione;IF: immunofluorescence; IHC: immunohistochemistry; IOP, intraocularpressure; IRI: ischemia-reperfusion injury; LAA: linoleamide alkyne;MDA: malondialdehyde; PGSK: Phen Green (TM) SK;RCD: regulatedcell death; PUFAs: polyunsaturated fatty acids; RFP: red fluorescentprotein;ROS: reactive oxygen species; TBA: thiobarbituricacid; TBARS: thiobarbituric acid reactive substances; TEM:transmission electron microscopy. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zoltán AU - Bajusz, Csaba AU - Szabó, Anikó AU - Borkúti, Péter AU - Vedelek, Balázs AU - Benke, Reka AU - Lipinszki, Zoltán AU - Kristó, Ildikó AU - Vilmos, Péter TI - A bipartite NLS motif mediates the nuclear import of Drosophila moesin JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 12 PY - 2024 PG - 14 SN - 2296-634X DO - 10.3389/fcell.2024.1206067 UR - https://m2.mtmt.hu/api/publication/34743202 ID - 34743202 N1 - Funding Agency and Grant Number: NKFIH (Hungarian National Research, Development and Innovation Office) through the National Laboratory for Biotechnology program [PD127968, LP2017-7/2017]; Hungarian Academy of Sciences Lendulet Grant; [2022-2.1.1-NL-2022-00008] Funding text: This work was supported by NKFIH (Hungarian National Research, Development and Innovation Office) through the National Laboratory for Biotechnology program, grant 2022-2.1.1-NL-2022-00008 (PV), and PD127968 (IK), and the Hungarian Academy of Sciences Lendulet Grant LP2017-7/2017 (ZL). AB - The ERM protein family, which consists of three closely related proteins in vertebrates, ezrin, radixin, and moesin (ERM), is an ancient and important group of cytoplasmic actin-binding and organizing proteins. With their FERM domain, ERMs bind various transmembrane proteins and anchor them to the actin cortex through their C-terminal F-actin binding domain, thus they are major regulators of actin dynamics in the cell. ERMs participate in many fundamental cellular processes, such as phagocytosis, microvilli formation, T-cell activation and tumor metastasis. We have previously shown that, besides its cytoplasmic activities, the single ERM protein of Drosophila melanogaster, moesin, is also present in the cell nucleus, where it participates in gene expression and mRNA export. Here we study the mechanism by which moesin enters the nucleus. We show that the nuclear import of moesin is an NLS-mediated, active process. The nuclear localization sequence of the moesin protein is an evolutionarily highly conserved, conventional bipartite motif located on the surface of the FERM domain. Our experiments also reveal that the nuclear import of moesin does not require PIP2 binding or protein activation, and occurs in monomeric form. We propose, that the balance between the phosphorylated and non-phosphorylated protein pools determines the degree of nuclear import of moesin. LA - English DB - MTMT ER - TY - JOUR AU - Laczkó-Dobos, Hajnalka AU - Bhattacharjee, Arindam AU - Maddali, Asha Kiran AU - Kincses, András AU - Abuammar, Hussein AU - Sebőkné Nagy, Krisztina AU - Páli, Tibor AU - Dér, András AU - Hegedűs, Tamás AU - Csordás, Gábor AU - Juhász, Gábor TI - PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion JF - AUTOPHAGY J2 - AUTOPHAGY VL - AiP PY - 2024 PG - 12 SN - 1554-8627 DO - 10.1080/15548627.2024.2322493 UR - https://m2.mtmt.hu/api/publication/34724664 ID - 34724664 N1 - Institute of Genetics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Doctoral School of Biology, University of Szeged, Szeged, Hungary Institute of Biophysics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary HUN-REN Biophysical Virology Research Group, Budapest, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Export Date: 27 March 2024 Correspondence Address: Juhász, G.; HUN-REN Biological Research Centre Szeged, Temesvari krt. 62, Hungary; email: juhasz.gabor@brc.hu LA - English DB - MTMT ER - TY - CHAP AU - Sávai, Gergő AU - Kartali, Tünde AU - Benci, Dániel Attila AU - Patai, Roland AU - Lipinszki, Zoltán AU - Vágvölgyi, Csaba AU - Papp, Tamás TI - Mikovírusok azonosítása Rhizopus fajokban T2 - Biotechnológiai Szakmai Nap Absztraktfüzet PB - Doktoranduszok Országos Szövetsége (DOSZ) CY - Budapest SN - 9786156457448 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34723493 ID - 34723493 LA - Hungarian DB - MTMT ER -