TY - JOUR AU - Govindjee, Govindjee AU - Amesz, Bas AU - Garab, Győző AU - Stirbet, Alexandrina TI - Remembering Jan Amesz (1934-2001): a great gentleman, a major discoverer, and an internationally renowned biophysicist of both oxygenic and anoxygenic photosynthesisa JF - PHOTOSYNTHESIS RESEARCH J2 - PHOTOSYNTH RES PY - 2024 PG - 18 SN - 0166-8595 DO - 10.1007/s11120-024-01102-9 UR - https://m2.mtmt.hu/api/publication/34857362 ID - 34857362 AB - We present here the research contributions of Jan Amesz (1934-2001) on deciphering the details of the early physico-chemical steps in oxygenic photosynthesis in plants, algae and cyanobacteria, as well as in anoxygenic photosynthesis in purple, green, and heliobacteria. His research included light absorption and the mechanism of excitation energy transfer, primary photochemistry, and electron transfer steps until the reduction of pyridine nucleotides. Among his many discoveries, we emphasize his 1961 proof, with L. N. M. Duysens, of the "series scheme" of oxygenic photosynthesis, through antagonistic effects of Light I and II on the redox state of cytochrome f. Further, we highlight the following research on oxygenic photosynthesis: the experimental direct proof that plastoquinone and plastocyanin function at their respective places in the Z-scheme. In addition, Amesz's major contributions were in unraveling the mechanism of excitation energy transfer and electron transport steps in anoxygenic photosynthetic bacteria (purple, green and heliobacteria). Before we present his research, focusing on his key discoveries, we provide a glimpse of his personal life. We end this Tribute with reminiscences from three of his former doctoral students (Sigi Neerken; Hjalmar Pernentier, and Frank Kleinherenbrink) and from several scientists (Suleyman Allakhverdiev; Robert Blankenship; Richard Cogdell) including two of the authors (G. Garab and A. Stirbet) of this Tribute. LA - English DB - MTMT ER - TY - JOUR AU - Porkoláb, Gergő AU - Mészáros, Mária AU - Szecskó, Anikó AU - Vigh, Judit Piroska AU - Walter, Fruzsina AU - Figueiredo, Ricardo AU - Kálomista, Ildikó AU - Hoyk, Zsófia AU - Vizsnyiczai, Gaszton AU - Gróf, Ilona AU - Jan, Jeng-Shiung AU - Gosselet, Fabien AU - Pirity, Melinda AU - Vastag, Monika AU - Hudson, Natalie AU - Campbell, Matthew AU - Veszelka, Szilvia AU - Deli, Mária Anna TI - Synergistic induction of blood–brain barrier properties JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 121 PY - 2024 IS - 21 SN - 0027-8424 DO - 10.1073/pnas.2316006121 UR - https://m2.mtmt.hu/api/publication/34856989 ID - 34856989 AB - Blood–brain barrier (BBB) models derived from human stem cells are powerful tools to improve our understanding of cerebrovascular diseases and to facilitate drug development for the human brain. Yet providing stem cell–derived endothelial cells with the right signaling cues to acquire BBB characteristics while also retaining their vascular identity remains challenging. Here, we show that the simultaneous activation of cyclic AMP and Wnt/β-catenin signaling and inhibition of the TGF-β pathway in endothelial cells robustly induce BBB properties in vitro. To target this interaction, we present a small-molecule cocktail named cARLA, which synergistically enhances barrier tightness in a range of BBB models across species. Mechanistically, we reveal that the three pathways converge on Wnt/β-catenin signaling to mediate the effect of cARLA via the tight junction protein claudin-5. We demonstrate that cARLA shifts the gene expressional profile of human stem cell–derived endothelial cells toward the in vivo brain endothelial signature, with a higher glycocalyx density and efflux pump activity, lower rates of endocytosis, and a characteristic endothelial response to proinflammatory cytokines. Finally, we illustrate how cARLA can improve the predictive value of human BBB models regarding the brain penetration of drugs and targeted nanoparticles. Due to its synergistic effect, high reproducibility, and ease of use, cARLA has the potential to advance drug development for the human brain by improving BBB models across laboratories. LA - English DB - MTMT ER - TY - JOUR AU - Böde, Kinga AU - Javornik, Uros AU - Dlouhy, Ondrej AU - Zsíros, Ottó AU - Biswas, Avratanu AU - Racskóné Domonkos, Ildikó AU - Sket, Primoz AU - Karlicky, Vaclav AU - Ughy, Bettina AU - Lambrev, Petar AU - Spunda, Vladimir AU - Plavec, Janez AU - Garab, Győző TI - Role of isotropic lipid phase in the fusion of photosystem II membranes JF - PHOTOSYNTHESIS RESEARCH J2 - PHOTOSYNTH RES PY - 2024 PG - 14 SN - 0166-8595 DO - 10.1007/s11120-024-01097-3 UR - https://m2.mtmt.hu/api/publication/34850122 ID - 34850122 N1 - Funding Agency and Grant Number: National Research Development and Innovation Office of Hungary (OTKA K) [128679]; Czech Science Foundation [GACR 23-07744]; European Union under the LERCO project via the Operational Programme Just Transition [CZ.10.03.01/00/22_003/0000003]; HUN-REN Biological Research Centre, Szeged Funding text: This work was supported by grants from the National Research Development and Innovation Office of Hungary (OTKA K 128679 to G.G.), the Czech Science Foundation (GACR 23-07744 S to G.G.) and the European Union under the LERCO project (CZ.10.03.01/00/22_003/0000003) via the Operational Programme Just Transition. CERIC-ERIC provided financial assistance for travel and lodging. Open access funding provided by HUN-REN Biological Research Centre, Szeged. AB - It has been thoroughly documented, by using P-31-NMR spectroscopy, that plant thylakoid membranes (TMs), in addition to the bilayer (or lamellar, L) phase, contain at least two isotropic (I) lipid phases and an inverted hexagonal (H-II) phase. However, our knowledge concerning the structural and functional roles of the non-bilayer phases is still rudimentary. The objective of the present study is to elucidate the origin of I phases which have been hypothesized to arise, in part, from the fusion of TMs (Garab et al. 2022 Progr Lipid Res 101,163). We take advantage of the selectivity of wheat germ lipase (WGL) in eliminating the I phases of TMs (Dlouh & yacute; et al. 2022 Cells 11: 2681), and the tendency of the so-called BBY particles, stacked photosystem II (PSII) enriched membrane pairs of 300-500 nm in diameter, to form large laterally fused sheets (Dunahay et al. 1984 BBA 764: 179). Our 31P-NMR spectroscopy data show that BBY membranes contain L and I phases. Similar to TMs, WGL selectively eliminated the I phases, which at the same time exerted no effect on the molecular organization and functional activity of PSII membranes. As revealed by sucrose-density centrifugation, magnetic linear dichroism spectroscopy and scanning electron microscopy, WGL disassembled the large laterally fused sheets. These data provide direct experimental evidence on the involvement of I phase(s) in the fusion of stacked PSII membrane pairs, and strongly suggest the role of non-bilayer lipids in the self-assembly of the TM system. LA - English DB - MTMT ER - TY - JOUR AU - Tan, Jue Yu Kelly AU - Chew, Liang Yuh AU - Juhász, Gábor AU - Yu, Fengwei TI - Interplay between autophagy and CncC regulates dendrite pruning in Drosophila JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 121 PY - 2024 IS - 10 SN - 0027-8424 DO - 10.1073/pnas.2310740121 UR - https://m2.mtmt.hu/api/publication/34841588 ID - 34841588 N1 - Funding Agency and Grant Number: Temasek Life Sciences Laboratory Singapore [TLL-2040] Funding text: ACKNOWLEDGMENTS. We thank E. H. Baehrecke, D. Bohmann, Guang-Chao Chen, H. Eaton, M.Gonzalez-Gaitan,Y.Jan, H. Jasper, A. L. Kolodkin, H. Kramer, J. Terman, T. Uemura, D. Williams, T. K. Kerppola, Bloomington Drosophila Stock Center, Developmental Studies Hybridoma Bank (University of Iowa) , and Vienna Drosophila Resource Center (Austria) for generously providing antibodies and fly stocks. We are grateful to E. H. Baehrecke for helpful discussion. This work was funded by Temasek Life Sciences Laboratory Singapore (TLL-2040) . AB - Autophagy is essential for the turnover of damaged organelles and long-lived proteins. It is responsible for many biological processes such as maintaining brain functions and aging. Impaired autophagy is often linked to neurodevelopmental and neurodegenerative diseases in humans. However, the role of autophagy in neuronal pruning during development remains poorly understood. Here, we report that autophagy regulates dendrite-specific pruning of ddaC sensory neurons in parallel to local caspase activation. Impaired autophagy causes the formation of ubiquitinated protein aggregates in ddaC neurons, dependent on the autophagic receptor Ref(2)P. Furthermore, the metabolic regulator AMP-activated protein kinase and the insulin–target of rapamycin pathway act upstream to regulate autophagy during dendrite pruning. Importantly, autophagy is required to activate the transcription factor CncC (Cap “n” collar isoform C), thereby promoting dendrite pruning. Conversely, CncC also indirectly affects autophagic activity via proteasomal degradation, as impaired CncC results in the inhibition of autophagy through sequestration of Atg8a into ubiquitinated protein aggregates. Thus, this study demonstrates the important role of autophagy in activating CncC prior to dendrite pruning, and further reveals an interplay between autophagy and CncC in neuronal pruning. LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Horti-Oravecz, Klaudia AU - Kövesdi, Dorottya AU - Bozsik, Anikó AU - Papp, Janos AU - Butz, Henriett AU - Patócs, Attila AU - Szebeni, Gábor AU - Grolmusz, Vince Kornél TI - Peripheral immunophenotyping reveals lymphocyte stimulation in healthy women living with hereditary breast and ovarian cancer syndrome JF - ISCIENCE J2 - ISCIENCE VL - AiP PY - 2024 SP - 109882 SN - 2589-0042 DO - 10.1016/j.isci.2024.109882 UR - https://m2.mtmt.hu/api/publication/34840271 ID - 34840271 LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Enikő AU - Faragó, Anna AU - Bodor, Gergely AU - Gémes, Nikolett AU - Puskás, László AU - Kovács, László AU - Szebeni, Gábor TI - Identification of immune subsets with distinct lectin binding signatures using multi-parameter flow cytometry: correlations with disease activity in systemic lupus erythematosus JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 PG - 22 SN - 1664-3224 DO - 10.3389/fimmu.2024.1380481 UR - https://m2.mtmt.hu/api/publication/34840255 ID - 34840255 LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Zvara, Ágnes AU - Bukovinszki, Vivien AU - Puskás, László AU - Balog, Attila AU - Szebeni, Gábor TI - Comparative single-cell multiplex immunophenotyping of therapy-naive patients with rheumatoid arthritis, systemic sclerosis, and systemic lupus erythematosus shed light on disease-specific composition of the peripheral immune system JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 PG - 22 SN - 1664-3224 DO - 10.3389/fimmu.2024.1376933 UR - https://m2.mtmt.hu/api/publication/34829278 ID - 34829278 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Office (NKFI), Hungary [GINOP-2.3.2-15-2016-00030, 2020-1.1.6-JVOdblac;-2021-00003, 2022-1.2.6-TT-IPARI-TR-2022-00023, 142877 FK22]; Jnos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/00582/22/8, NKP-23-5-SZTE-694]; New National Excellence Program of the Ministry for Innovation and Technology Funding text: The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This research was funded by the GINOP-2.3.2-15-2016-00030, 2020-1.1.6-JOV & Odblac;-2021-00003, 2022-1.2.6-TET-IPARI-TR-2022-00023, and 142877 FK22 grants from the National Research, Development, and Innovation Office (NKFI), Hungary. This work was supported by an SZTE OK-KKA Hetenyi 2020 grant (AB). This work was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences BO/00582/22/8 (GS) and the & Uacute;NKP-23-5-SZTE-694 New National Excellence Program of the Ministry for Innovation and Technology (GS). LA - English DB - MTMT ER - TY - JOUR AU - Páli, Tibor AU - Feniouk, Boris AU - Wilkens, Stephan TI - Editorial: Functions, working mechanisms, and regulation of rotary ATPases and Ductin proteins JF - FRONTIERS IN MOLECULAR BIOSCIENCES J2 - FRONT MOL BIOSCI VL - 11 PY - 2024 SN - 2296-889X DO - 10.3389/fmolb.2024.1399421 UR - https://m2.mtmt.hu/api/publication/34812598 ID - 34812598 LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Fodor, K. AU - Magyar, Lilla Brigitta AU - Lipinszki, Zoltán AU - Hultmark, D. AU - Andó, István TI - Cellular Immunity of Drosophila willistoni Reveals Novel Complexity in Insect Anti-Parasitoid Defense JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 7 SN - 2073-4409 DO - 10.3390/cells13070593 UR - https://m2.mtmt.hu/api/publication/34804520 ID - 34804520 N1 - Innate Immunity Group, Institute of Genetics, HUN-REN Biological Research Centre, Szeged, 6726, Hungary Doctoral School of Biology, University of Szeged, Szeged, 6720, Hungary MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, 6726, Hungary National Laboratory for Biotechnology, Institute of Genetics, HUN-REN Biological Research Centre, Szeged, 6726, Hungary Department of Molecular Biology, Umea University, Umea, 901 87, Sweden Export Date: 22 April 2024 Correspondence Address: Cinege, G.; Innate Immunity Group, Hungary; email: cinege.gyongyi@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Hudhud, Lina AU - Kovács-Rozmer, Katalin AU - Kecskés, Angéla AU - Pohóczky, Krisztina AU - Bencze, Noémi AU - Buzás, Krisztina AU - Szőke, Éva AU - Helyes, Zsuzsanna TI - Transient Receptor Potential Ankyrin 1 Ion Channel Is Expressed in Osteosarcoma and Its Activation Reduces Viability JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 13 SN - 1661-6596 DO - 10.3390/ijms25073760 UR - https://m2.mtmt.hu/api/publication/34797924 ID - 34797924 N1 - * Megosztott szerzőség AB - Osteosarcoma is a highly malignant, painful cancer with poor treatment opportunities and a bad prognosis. Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are non-selective cation channels that have been of great interest in cancer, as their expression is increased in some malignancies. In our study we aim to characterize the expression and functionality of the TRPA1 and TRPV1 channels in human and mouse osteosarcoma tissues and in a mouse cell line. TRPA1/Trpa1 and TRPV1/Trpv1 mRNA expressions were demonstrated by PCR gel electrophoresis and RNAscope in situ hybridization. The function of these channels was confirmed by their radioactive 45Ca2+ uptake in response to the TRPA1 agonist, Allyl-isothiocyanate (AITC), and TRPV1 agonist, capsaicin, in K7M2 cells. An ATP-based K2M7 cell viability luminescence assay was used to determine cell viability after AITC or capsaicin treatments. Both TRPA1/Trpa1 and TRPV1/Trpv1 were expressed similarly in human and mouse osteosarcoma tissues, while Trpa1 transcripts were more abundantly present in K7M2 cells. TRPA1 activation with 200 µM AITC induced a significant 45Ca2+ influx into K7M2 cells, and the antagonist attenuated this effect. In accordance with the lower Trpv1 expression, capsaicin induced a moderate 45Ca2+ uptake, which did not reach the level of statistical significance. Both AITC and capsaicin significantly reduced K7M2 cell viability, demonstrating EC50 values of 22 µM and 74 µM. The viability-decreasing effect of AITC was significantly but only partially antagonized by HC-030031, but the action of capsaicin was not affected by the TRPV1 antagonist capsazepine. We provide here the first data on the functional expression of the TRPA1 and TRPV1 ion channels in osteosarcoma, suggesting novel diagnostic and/or therapeutic perspectives. LA - English DB - MTMT ER -