TY - JOUR AU - György, Bernadett AU - Pálóczi, Krisztina AU - Balbisi, Mirjam AU - Turiák, Lilla AU - Drahos, László AU - Visnovitz, Tamás AU - Koltai, Erika AU - Radák, Zsolt TI - Effect of the 35 nm and 70 nm Size Exclusion Chromatography (SEC) Column and Plasma Storage Time on Separated Extracellular Vesicles JF - CURRENT ISSUES IN MOLECULAR BIOLOGY J2 - CURR ISSUES MOL BIOL VL - 46 PY - 2024 IS - 5 SP - 4337 EP - 4357 PG - 21 SN - 1467-3037 DO - 10.3390/cimb46050264 UR - https://m2.mtmt.hu/api/publication/34850199 ID - 34850199 AB - The technical difficulty of separating extracellular vesicles (EVs) from plasma proteins in human blood presents a significant hurdle in EV research, particularly during nano ultra-high-performance liquid chromatography–tandem mass spectrometric (UHPLC-MS/MS) analysis, where detecting “vesicular” proteins among abundant plasma proteins is challenging. Standardisation is a pressing issue in EV research, prompting collaborative global efforts to address it. While the MISEV guidelines offer valuable recommendations, unanswered questions remain, particularly regarding sample storage. We compared size exclusion chromatography (SEC) columns with pore sizes of 35 nm and 70 nm to identify fractions with minimal contaminating proteins and the highest concentration of small EVs (sEVs). Following column selection, we explored potential differences in the quality and quantity of sEVs isolated from platelet-free plasma (PFP) after long-term storage at −80 °C (>2.5 years) compared to freshly drawn blood. Our methodologically rigorous study indicates that prolonged storage, under correct storage and processing conditions, does not compromise sEV quality. Both columns effectively isolated vesicles, with the 70 nm column exhibiting a higher abundance of “vesicular” proteins. We propose a relatively rapid and moderately efficient protocol for obtaining a comparatively pure sEV fraction from plasma, facilitating sEV processing in clinical trials. LA - English DB - MTMT ER - TY - JOUR AU - Powers, Scott K. AU - Radák, Zsolt AU - Ji, Li Li AU - Jackson, Malcolm TI - Reactive oxygen species promote endurance exercise-induced adaptations in skeletal muscles JF - JOURNAL OF SPORT AND HEALTH SCIENCE J2 - JSHS PY - 2024 SN - 2095-2546 DO - 10.1016/j.jshs.2024.05.001 UR - https://m2.mtmt.hu/api/publication/34850193 ID - 34850193 N1 - Available online 7 May 2024 AB - The discovery that contracting skeletal muscle generates reactive oxygen species (ROS) was first reported over 40 years ago. The prevailing view in the 1980s was that exercise-induced ROS production promotes oxidation of proteins and lipids resulting in muscle damage. However, a paradigm shift occurred in the 1990s as growing research revealed that ROS are signaling molecules, capable of activating transcriptional activators/coactivators and promoting exercise-induced muscle adaptation. Growing evidence supports the notion that reduction-oxidation (redox) signaling pathways play an important role in the muscle remodeling that occurs in response to endurance exercise training. This review examines the specific role that redox signaling plays in this endurance exercise-induced skeletal muscle adaptation. We begin with a discussion of the primary sites of ROS production in contracting muscle fibers followed by a summary of the antioxidant enzymes involved in the regulation of ROS levels in the cell. We then discuss which redox-sensitive signaling pathways promote endurance exercise-induced muscle adaptation and debate the strength of the evidence supporting the notion that redox signaling plays an essential role in muscle adaptation to endurance exercise training. In hopes of stimulating future research, we highlight several important unanswered questions in this field. LA - English DB - MTMT ER - TY - JOUR AU - Kawamura, T. AU - Kerepesi, C. AU - Torma, Ferenc Gergely AU - Bori, Z. AU - Zhou, L. AU - Bakonyi, Péter AU - Radák, Zsolt TI - Organ specificity and commonality of epigenetic aging in low- and high-running capacity rats JF - bioRxive VL - 2024 PY - 2024 SN - 2692-8205 DO - 10.1101/2024.04.21.590009 UR - https://m2.mtmt.hu/api/publication/34829424 ID - 34829424 LA - English DB - MTMT ER - TY - JOUR AU - OKAMOTO, MASAHIRO AU - SHIMODA, RYO AU - AMAYA, YUKI AU - SOYA, SHINGO AU - SOYA, MARIKO AU - KOIZUMI, HIKARU AU - NAKAMURA, KENGO AU - HIRAGA, TAICHI AU - Torma, Ferenc Gergely AU - SOYA, HIDEAKI TI - Accelerated Fear Extinction by Regular Light-Intensity Exercise: A Possible Role of Hippocampal BDNF-TrkB Signaling JF - MEDICINE AND SCIENCE IN SPORTS AND EXERCISE J2 - MED SCI SPORT EXER VL - 56 PY - 2024 IS - 2 SP - 221 EP - 229 PG - 9 SN - 0195-9131 DO - 10.1249/MSS.0000000000003312 UR - https://m2.mtmt.hu/api/publication/34776881 ID - 34776881 LA - English DB - MTMT ER - TY - JOUR AU - Ayaz, Asma AU - Zaman, Wajid AU - Radák, Zsolt AU - Gu, Yaodong TI - Harmony in Motion: Unraveling the Nexus of Sports, Plant-Based Nutrition, and Antioxidants for Peak Performance JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 13 PY - 2024 IS - 4 SP - 437 SN - 2076-3921 DO - 10.3390/antiox13040437 UR - https://m2.mtmt.hu/api/publication/34775640 ID - 34775640 AB - The intricate interplay between plant-based nutrition, antioxidants, and their impact on athletic performance forms the cornerstone of this comprehensive review. Emphasizing the pivotal importance of dietary choices in the realm of sports, this paper sets the stage for an in-depth exploration of how stress and physical performance are interconnected through the lens of nutrition. The increasing interest among athletes in plant-based diets presents an opportunity with benefits for health, performance, and recovery. It is essential to investigate the connection between sports, plants, and antioxidants. Highlighting the impact of nutrition on recovery and well-being, this review emphasizes how antioxidants can help mitigate oxidative stress. Furthermore, it discusses the growing popularity of plant-based diets among athletes. It elaborates on the importance of antioxidants in combating radicals addressing stress levels while promoting cellular health. By identifying rich foods, it emphasizes the role of a balanced diet in ensuring sufficient intake of these beneficial compounds. Examining stress within the context of sports activities, this review provides insights into its mechanisms and its impact on athletic performance as well as recovery processes. This study explores the impact of plant-based diets on athletes including their types, potential advantages and challenges. It also addresses the drawbacks of relying on plant-based diets, concerns related to antioxidant supplementation and identifies areas where further research is needed. Furthermore, the review suggests directions for research and potential innovations in sports nutrition. Ultimately it brings together the aspects of sports, plant-based nutrition, and antioxidants to provide a perspective for athletes, researchers and practitioners. By consolidating existing knowledge, it offers insights that can pave the way for advancements in the ever-evolving field of sports nutrition. LA - English DB - MTMT ER - TY - JOUR AU - Kolonics, Attila AU - Kawamura, Takuji AU - Szipőcs, Róbert AU - Radák, Zsolt TI - Combined NAD(P)H and lipofuscin FLIM revealed the development of metabolic syndrome in the liver of epigenetically altered rats JF - PROCEEDINGS OF SPIE - THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING J2 - PROCEEDINGS OF SPIE VL - 12849 PY - 2024 PG - 9 SN - 0277-786X DO - 10.1117/12.3005632 UR - https://m2.mtmt.hu/api/publication/34743288 ID - 34743288 LA - English DB - MTMT ER - TY - JOUR AU - Olek, Robert A. AU - Kujach, Sylwester AU - Radák, Zsolt TI - Current knowledge about pyruvate supplementation: A brief review JF - SPORTS MEDICINE AND HEALTH SCIENCE J2 - SPORTS MED HEALTH SCI VL - 2024 PY - 2024 SN - 2666-3376 DO - 10.1016/j.smhs.2024.02.007 UR - https://m2.mtmt.hu/api/publication/34718895 ID - 34718895 LA - English DB - MTMT ER - TY - JOUR AU - Oroszi, Tamás AU - Felszeghy, Klára AU - Luiten, Paul G.M. AU - Schoemaker, Regien G. AU - van der Zee, Eddy A. AU - Nyakas, Csaba TI - Whole body vibration ameliorates anxiety-like behavior and memory functions in 30 months old senescent male rats JF - HELIYON J2 - HELIYON VL - 10 PY - 2024 IS - 4 PG - 11 SN - 2405-8440 DO - 10.1016/j.heliyon.2024.e26608 UR - https://m2.mtmt.hu/api/publication/34714793 ID - 34714793 N1 - Department of Molecular Neurobiology, Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, Groningen, Netherlands Research Center for Molecular Exercise Science, Hungarian University of Sports Science, Budapest, Hungary Department of Morphology and Physiology, Health Science Faculty, Semmelweis University, Budapest, Hungary Export Date: 10 April 2024 Correspondence Address: Oroszi, T.; Department of Molecular Neurobiology, Netherlands; email: t.oroszi@rug.nl LA - English DB - MTMT ER - TY - JOUR AU - Torma, Ferenc Gergely AU - Kerepesi, Csaba AU - Jókai, Mátyás AU - Bábszky, Gergely AU - Koltai, Erika AU - Ligeti, Balázs AU - Kalcsevszki, Regina AU - McGreevy, Kristen M. AU - Horvath, Steve AU - Radák, Zsolt TI - Alterations of the gut microbiome are associated with epigenetic age acceleration and physical fitness JF - AGING CELL J2 - AGING CELL VL - 23 PY - 2024 IS - 4 PG - 12 SN - 1474-9718 DO - 10.1111/acel.14101 UR - https://m2.mtmt.hu/api/publication/34693196 ID - 34693196 N1 - Funding Agency and Grant Number: European Union; National Research, Development and Innovation Office, Hungary; National Excellence Program [126823]; National Science and Research Found [OTKA142192, TKP2021-EGA-37]; Hungarian University Sport Science, Innovation and Technology Ministry, Hungary [2021-28]; National Academy of Science, Hungary; [RRF-2.3.1-21-2022-00004]; [OTKA146113] Funding text: CK was supported by the European Union project RRF-2.3.1-21-2022-00004 within the framework of the Artificial Intelligence National Laboratory, and the National Excellence Program (OTKA146113) of the National Research, Development and Innovation Office, Hungary. ZR acknowledges support from the National Excellence Program (126823) National Science and Research Found (OTKA142192) and Scientific Excellence Program TKP2021-EGA-37 at the Hungarian University Sport Science, Innovation and Technology Ministry, Hungary, as well as Post-Covid 2021-28 grant by National Academy of Science, Hungary. We thank to Szilvia Farkas (Semmelweis University, Hungary) for the valuable discussions. AB - Epigenetic clocks can measure aging and predict the incidence of diseases and mortality. Higher levels of physical fitness are associated with a slower aging process and a healthier lifespan. Microbiome alterations occur in various diseases and during the aging process, yet their relation to epigenetic clocks is not explored. To fill this gap, we collected metagenomic (from stool), epigenetic (from blood), and exercise‐related data from physically active individuals and, by applying epigenetic clocks, we examined the relationship between gut flora, blood‐based epigenetic age acceleration, and physical fitness. We revealed that an increased entropy in the gut microbiome of physically active middle‐aged/old individuals is associated with accelerated epigenetic aging, decreased fitness, or impaired health status. We also observed that a slower epigenetic aging and higher fitness level can be linked to altered abundance of some bacterial species often linked to anti‐inflammatory effects. Overall our data suggest that alterations in the microbiome can be associated with epigenetic age acceleration and physical fitness. LA - English DB - MTMT ER - TY - JOUR AU - Kizilay, F. AU - Emin, Kafkas M. AU - Çagatay, Tąskapan M. AU - Haydar, Demirel A. AU - Radák, Zsolt TI - Impact of differing eccentric-concentric phase durations on muscle damage and anabolic hormones JF - ISOKINETICS AND EXERCISE SCIENCE J2 - ISOKINET EXERC SCI VL - 32 PY - 2024 IS - 1 SP - 29 EP - 39 PG - 11 SN - 0959-3020 DO - 10.3233/IES-220078 UR - https://m2.mtmt.hu/api/publication/34596630 ID - 34596630 LA - English DB - MTMT ER -