TY - JOUR AU - Török, Dóra AU - Somoskői, Bence AU - Bordás, Lilla AU - Reglődi, Dóra AU - Cseh, Sándor TI - Effect of pituitary adenylate cyclase-activating polypeptide supplementation, applied during or after vitrification on mouse embryo JF - ACTA VETERINARIA HUNGARICA J2 - ACTA VET HUNG VL - 71 PY - 2023 IS - 2 SP - 112 EP - 118 PG - 7 SN - 0236-6290 DO - 10.1556/004.2023.00903 UR - https://m2.mtmt.hu/api/publication/34133721 ID - 34133721 N1 - Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [2020-4.1.1-TKP2020, 134887]; Thematic Excellence Program 2021 Health Sub-programme of the Ministry for Innovation and Technology in Hungary [FK_20] Funding text: The project was supported by the Hungarian Research Fund (OTKA 115874); NKFIHK135457; TKP2020-IKA-08,MTA-TKI-14016. Project no. TKP2020-NKA-01 has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the Tematerueleti Kivalosagi Program 2020 (2020-4.1.1-TKP2020) funding scheme. Project no. 134887 has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the FK_20 funding scheme. Thematic Excellence Program 2021 Health Sub-programme of the Ministry for Innovation and Technology in Hungary,within the framework of the EGA-16 project of the University Pecs.r financed under the Temateruleti Kivalosagi Program 2020 (2020-4.1.1-TKP2020) funding scheme. Project no. 134887 has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the FK_20 funding scheme. Thematic Excellence Program 2021 Health Sub-programme of the Ministry for Innovation and Technology in Hungary, within the framework of the EGA-16 project of the Uni-versity Pecs. AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. It occurs in high levels in the gonads suggesting a potential central role in reproduction. The aim of our study was to assess the effect of PACAP treatment during embryo vitrification on the developmental rate and the expression of the heparin-binding EGF-like growth factor gene (Hbegf). Mouse embryos, obtained from superovulated females were allocated into the four treatment groups. In EM1 and EM2, the embryos were prepared for vitrification in an Equilibration Solution that was supplemented with 1 or 2 μM PACAP1-38, respectively. The embyos in groups CM1 and CM2 were not treated prior to vitrification but were cultured in a medium supplemented with 1 or 2 μM PACAP1-38 after thawing. The Vitrified Control group consisted of embryos vitrified and thawed then cultured without PACAP1-38 treatment. A non-vitrified, non-treated Fresh Control group was also used. After 24 h of culture, the developmental rate of the embryos, as well as the relative expression level of the Hbegf gene, as determined by qPCR, were compared among groups. Higher developmental rate and Hbegf gene expression level were found in the embryos treated with a higher concentration of PACAP. These results indicate that PACAP treatment has a beneficial effect on the survival and development of vitrified/thawed mouse embryos. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Tünde AU - Alizadeh, Hussain AU - Polgár, Beáta AU - Csalódi, Renáta AU - Reglődi, Dóra AU - Tamás, Andrea TI - Diagnostic and Prognostic Value of PACAP in Multiple Myeloma JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 13 PG - 27 SN - 1661-6596 DO - 10.3390/ijms241310801 UR - https://m2.mtmt.hu/api/publication/34067933 ID - 34067933 AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) is a multifunctional neuropeptide with well-known anti-inflammatory, antioxidant, antitumor, and immunomodulatory effects. PACAP regulates the production of various proinflammatory factors and may influence the complex cytokine network of the bone marrow microenvironment altered by plasma cells, affecting the progression of multiple myeloma (MM) and the development of end-organ damage. The aim of our study was to investigate the changes in PACAP-38 levels in patients with MM to explore its value as a potential biomarker in this disease. We compared the plasma PACAP-38 levels of MM patients with healthy individuals by ELISA method and examined its relationship with various MM-related clinical and laboratory parameters. Lower PACAP-38 levels were measured in MM patients compared with the healthy controls, however, this difference vanished if the patient achieved any response better than partial response. In addition, lower peptide levels were found in elderly patients. Significantly higher PACAP-38 levels were seen in patients with lower stage, lower plasma cell infiltration in bone marrow, lower markers of tumor burden in serum, lower total urinary and Bence-Jones protein levels, and in patients after lenalidomide therapy. Higher PACAP-38 levels in newly diagnosed MM patients predicted longer survival and a higher probability of complete response to treatment. Our findings confirm the hypothesis that PACAP plays an important role in the pathomechanism of MM. Furthermore, our results suggest that PACAP might be used as a valuable, non-invasive, complementary biomarker in diagnosis, and may be utilized for prognosis prediction and response monitoring. LA - English DB - MTMT ER - TY - JOUR AU - D'Amico, Agata Grazia AU - Maugeri, Grazia AU - Magrì, Benedetta AU - Giunta, Salvatore AU - Saccone, Salvatore AU - Federico, Concetta AU - Pricoco, Elisabetta AU - Broggi, Giuseppe AU - Caltabiano, Rosario AU - Musumeci, Giuseppe AU - Reglődi, Dóra AU - D'Agata, Velia TI - Modulatory activity of ADNP on the hypoxia‑induced angiogenic process in glioblastoma JF - INTERNATIONAL JOURNAL OF ONCOLOGY J2 - INT J ONCOL VL - 62 PY - 2023 IS - 1 PG - 10 SN - 1019-6439 DO - 10.3892/ijo.2022.5462 UR - https://m2.mtmt.hu/api/publication/33453105 ID - 33453105 N1 - * Megosztott szerzőség AB - Glioblastoma multiforme (GBM) is a brain cancer with a poor prognosis that affects adults. This is a solid tumor characterized by a high rate of cell migration and invasion. The uncontrolled cell proliferation creates hypoxic niches in the tumor mass, which leads to the overexpression of hypoxia‑inducible factors (HIFs). This induces the activation of the vascular endothelial growth factor (VEGF), which is responsible for uncontrolled neoangiogenesis. Recent studies have demonstrated the anti‑invasive effect of pituitary adenylate cyclase‑activating peptide (PACAP) in GBM. PACAP effects on the central nervous system are also mediated through the activity‑dependent neuroprotective protein (ADNP) activation. To date, no evidence exists regarding its role in GBM. Therefore, the ADNP involvement in GBM was investigated. By analyzing ADNP expression in a human GBM sample through confocal microscopy, a high ADNP immunoreactivity was detected in most glial cells and its predominant expression in hypoxic areas overexpressing HIF‑1α was highlighted. To investigate the role of ADNP on the HIF‑VEGF axis in GBM, a human U87MG GBM cell line was cultured with a hypoxic mimetic agent, deferoxamine, and cells were treated with the smallest active fragment of ADNP, known as NAP. The protein expression and distribution of HIF‑1α and VEGF was detected using western blot analysis and immunofluorescence assay. Results demonstrated that ADNP modulates the hypoxic‑angiogenic pathway in GBM cells by reducing VEGF secretion, detected through ELISA assay, as well as modulating their migration, assessed through wound healing assay. Although deeper investigation is necessary, the present study suggested that ADNP could be involved in PACAP anti‑invasive effects in GBM. LA - English DB - MTMT ER - TY - CHAP AU - Patkó, Evelin Viktória AU - Szabó, Edina AU - Váczy, Alexandra AU - Molitor, Dorottya AU - Eniko, Tari AU - Lina, Li AU - Adrienne, Csutak AU - Gabor, Toth AU - Reglődi, Dóra AU - Atlasz, Tamás ED - Kajos, Luca Fanni ED - Bali, Cintia ED - Puskás, Tamás ED - Polgár, Petra Ibolya ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám ED - Kovács, Eszter TI - The protective effects of PACAP1-38 on the retinal vasculature and hypoxic molecules in rat glaucoma model T2 - XI. Interdiszciplináris Doktorandusz Konferencia 2022. november 25-26 = 11th Interdisciplinary Doctoral Conference 25-26th of November 2022 PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat CY - Pécs SN - 9789636260699 PY - 2022 SP - 130 PG - 1 UR - https://m2.mtmt.hu/api/publication/33551214 ID - 33551214 LA - English DB - MTMT ER - TY - CHAP AU - Mérész, Balázs AU - Molitor, Dorottya AU - Schimdth, Anne AU - Patkó, Evelin Viktória AU - Szabó, Edina AU - Li, Lina AU - Reglődi, Dóra AU - Atlasz, Tamás AU - Váczy, Alexandra ED - Prisztóka, Gyöngyvér ED - Kertai, Bendegúz TI - Terhesség alatti dohányzás hatása a koraszülött retinopáthiára = The effects of maternal smoking on retinopathy of prematurity T2 - XX. Szentágothai János Mutidiszciplináris Konferencia és Hallgatói Verseny Absztrakt kötet = XX. János Szentágothai Multidisciplinary Conference and Student Competition PB - PTE TTK Szentágothai János Szakkollégium, Tehetségpont és Egyesület CY - Pécs SN - 9789634299554 PY - 2022 SP - 161 EP - 162 PG - 2 UR - https://m2.mtmt.hu/api/publication/32831203 ID - 32831203 LA - English DB - MTMT ER - TY - CHAP AU - Tari, Enikő AU - Patkó, Evelin Viktória AU - Szabó, Edina AU - Váczy, Alexandra AU - Molitor, Dorottya AU - Lina, Li AU - Csutak, Adrienne AU - Tóth, Gábor AU - Reglődi, Dóra AU - Atlasz, Tamás ED - Prisztóka, Gyöngyvér ED - Kertai, Bendegúz TI - A PACAP 1-38 potenciális védőhatása a retina erezettségére patkány glaukóma modellben = The possible protective effects of PACAP 1-38 on the retinal vasculature in glaucomatous rat model T2 - XX. Szentágothai János Mutidiszciplináris Konferencia és Hallgatói Verseny Absztrakt kötet = XX. János Szentágothai Multidisciplinary Conference and Student Competition PB - PTE TTK Szentágothai János Szakkollégium, Tehetségpont és Egyesület CY - Pécs SN - 9789634299554 PY - 2022 SP - 153 EP - 154 PG - 2 UR - https://m2.mtmt.hu/api/publication/32831178 ID - 32831178 LA - English DB - MTMT ER - TY - JOUR AU - Horváth-Opper, Gabriella AU - Reglődi, Dóra AU - Fábián, Eszter AU - Opper, Balázs TI - Effects of Pituitary Adenylate Cyclase Activating Polypeptide on Cell Death JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 9 PG - 20 SN - 1661-6596 DO - 10.3390/ijms23094953 UR - https://m2.mtmt.hu/api/publication/32799467 ID - 32799467 N1 - Funding Agency and Grant Number: Thematic Excellence Program 2021 Health Sub-program of the Ministry for Innovation and Technology in Hungary of the Pecs of University; National Research, Development, and Innovation Fund [FK129190, K119759, K135457]; National Brain Research Program [NAP20171.2.1-NKP-2017-00002]; Higher Education Institutional Excellence Program of the Ministry of Human Capacities in Hungary [MTA-TKI-14016, GINOP-2.3.2-15-2016-00050]; National Research, Development, and Innovation Fund of Hungary [20765/3/2018/FEKUTSTRAT, 2020-4.1.1-TKP2020-FIKP III, TKP2020-IKA-08] Funding text: The research was financed by the Thematic Excellence Program 2021 Health Sub-program of the Ministry for Innovation and Technology in Hungary, within the framework of the EGA-16 project of the Pecs of University. This study was supported by the National Research, Development, and Innovation Fund (FK129190, K119759, K135457); National Brain Research Program (NAP20171.2.1-NKP-2017-00002); MTA-TKI-14016; GINOP-2.3.2-15-2016-00050 "PEPSYS"; "The role of neuroinflammation in neurodegeneration: from molecules to clinics"; and Higher Education Institutional Excellence Program of the Ministry of Human Capacities in Hungary: 20765/3/2018/FEKUTSTRAT, 2020-4.1.1-TKP2020-FIKP III. Project No. TKP2020-IKA-08 was implemented with the support provided from the National Research, Development, and Innovation Fund of Hungary, financed under the 2020-4.1.1-TKP2020 funding scheme. AB - Pituitary adenylate cyclase activating polypeptide (PACAP) was first isolated as a hypothalamic peptide based on its efficacy to increase adenylate cyclase (AC) activity. It has a widespread distribution throughout the body including the nervous system and peripheral organs, where PACAP exerts protective effects both in vivo and in vitro through its anti-apoptotic, anti-inflammatory, and antioxidant functions. The aim of the present paper was to review the currently available literature regarding the effects of PACAP on cell death in vitro in neural and non-neural cells. Among others, its effect on apoptosis can be detected in cerebellar granule cells against different toxic stimuli. Different neural cell types from the cerebral cortex are also prevented from cell death. PACAP also shows effects on cell death in cells belonging to the peripheral nervous system and protects both neural and non-neural cells of sensory organs. In addition, cell survival-promoting effect can be observed in different peripheral organ systems including cardiovascular, immune, respiratory, gastrointestinal, urinary, and reproductive systems. The studies summarized here indicate its noteworthy effect on cell death in different in vitro models, suggesting PACAP's potential therapeutic usage in several pathological conditions. LA - English DB - MTMT ER - TY - JOUR AU - Szegeczki, Vince AU - Fazekas, László Ádám AU - Kulcsár, Máté AU - Reglődi, Dóra AU - Török, Péter AU - Orlik, Brigitta AU - Laganà, Antonio S. AU - Jakab, Attila AU - Juhász, Tamás TI - Endometrium as Control of Endometriosis in Experimental Research: Assessment of Sample Suitability JF - DIAGNOSTICS J2 - DIAGNOSTICS VL - 12 PY - 2022 IS - 4 PG - 15 SN - 2075-4418 DO - 10.3390/diagnostics12040970 UR - https://m2.mtmt.hu/api/publication/32785459 ID - 32785459 AB - Endometriosis is a chronic gynecological disease that causes numerous severe symptoms in affected women. Revealing alterations of the molecular processes in ectopic endometrial tissue is the current policy for understanding the pathomechanisms and discovering potential novel therapeutic targets. Examining molecular processes of eutopic endometrium is likely to be a convenient method to compare it with the molecular alterations observed in ectopic tissues. The aim of the present study was to determine what proportion of the surgically resected eutopic endometrial samples is suitable for further experiments so that these can be comparable with endometriosis. Final hospital reports and histopathology reports of a 3-year-long period (1162 cases) were analysed. The application of a retrospective screening method promoted the categorization of these cases, and quantification of the categorized cases was accomplished. In addition, results obtained from cultured endometrium samples were also detailed. Only a small number of the harvested endometrial samples was suitable for further molecular analysis, while preoperative screening protocol could enlarge this fraction. Applying clinical and histopathological selection and exclusion criteria for tissue screening and histopathological examination of samples could ensure the comparability of healthy endometrium with endometriosis. The present study could be useful for researchers who intend to perform molecular experiments to compare endometriosis with the physiological processes of the endometrium. LA - English DB - MTMT ER - TY - JOUR AU - Kiss, Péter AU - Farkas, József AU - Kovács, Krisztina AU - Gaál, Valéria AU - Biró, Zsolt AU - Szabó, Aliz AU - Atlasz, Tamás AU - Bosnyak, Inez AU - Tóth, Gábor AU - Tamás, Andrea AU - Reglődi, Dóra TI - Effects of pituitary adenylate cyclase activating polypeptide (PACAP) in corneal epithelial regeneration and signal transduction in rats JF - INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS J2 - INT J PEPT RES THER VL - 28 PY - 2022 IS - 3 PG - 9 SN - 1573-3149 DO - 10.1007/s10989-022-10405-1 UR - https://m2.mtmt.hu/api/publication/32776007 ID - 32776007 LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Dóra AU - Sárszegi, Zsolt AU - Polgár, Beáta AU - Sághy, Éva AU - Reglődi, Dóra AU - Tóth, Tünde AU - Onódi, Zsófia AU - Leszek, Przemyslaw AU - Varga, Zoltán AU - Helyes, Zsuzsanna AU - Kemény, Ágnes AU - Ferdinandy, Péter AU - Tamás, Andrea TI - PACAP-38 and PAC1 Receptor Alterations in Plasma and Cardiac Tissue Samples of Heart Failure Patients JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 7 PG - 18 SN - 1661-6596 DO - 10.3390/ijms23073715 UR - https://m2.mtmt.hu/api/publication/32763181 ID - 32763181 N1 - * Megosztott szerzőség LA - English DB - MTMT ER -