@article{MTMT:34856915, title = {Triggered cagedSTORM microscopy}, url = {https://m2.mtmt.hu/api/publication/34856915}, author = {Bíró, Péter and Novák, Tibor and Czvik, Elvira and Mihály, József and Szikora, Szilárd and van de Linde, Sebastian and Erdélyi, Miklós}, doi = {10.1364/BOE.517480}, journal-iso = {BIOMED OPT EXPRESS}, journal = {BIOMEDICAL OPTICS EXPRESS}, volume = {15}, unique-id = {34856915}, issn = {2156-7085}, abstract = {In standard SMLM methods, the photoswitching of single fluorescent molecules and the data acquisition processes are independent, which leads to the detection of single molecule blinking events on several consecutive frames. This mismatch results in several data points with reduced localization precision, and it also increases the possibilities of overlapping. Here we discuss how the synchronization of the fluorophores’ ON state to the camera exposure time increases the average intensity of the captured point spread functions and hence improves the localization precision. Simulations and theoretical results show that such synchronization leads to fewer localizations with 15% higher sum signal on average, while reducing the probability of overlaps by 10%.}, year = {2024}, pages = {3715-3726}, orcid-numbers = {Novák, Tibor/0000-0003-0756-6851; Erdélyi, Miklós/0000-0002-9501-5752} } @article{MTMT:34815538, title = {Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing}, url = {https://m2.mtmt.hu/api/publication/34815538}, author = {Lanszki, Zsófia and Islam, Md. Shafeul and Shikder, Md. Foisal and Sarder, Md. Jalal Uddin and Khan, Shahneaz Ali and Chowdhury, Sharmin and Islam, Md. Nurul and Tauber, Zsófia and Tóth, Gábor Endre and Jakab, Ferenc and Kemenesi, Gábor and Akter, Sazeda}, doi = {10.1038/s41598-024-59343-6}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34815538}, issn = {2045-2322}, abstract = {Canine distemper virus (CDV) is a highly contagious virus that affects domestic and wild animals, causing severe illness with high mortality rates. Rapid monitoring and sequencing can provide valuable information about circulating CDV strains, which may foster effective vaccination strategies and the successful integration of these into conservation programs. During two site visits in Bangladesh in 2023, we tested a mobile, deployable genomic surveillance setup to explore the genetic diversity and phylogenetic patterns of locally circulating CDV strains. We collected and analysed 355 oral swab samples from stray dogs in Rajshahi and Chattogram cities, Bangladesh. CDV-specific real-time RT-PCR was performed to screen the samples. Out of the 355 samples, 7.4% (10/135) from Rajshahi city and 0.9% (2/220) from Chattogram city tested positive for CDV. We applied a real-time RT-PCR assay and a pan-genotype CDV-specific amplicon-based Nanopore sequencing technology to obtain the near-completes. Five near-complete genome sequences were generated, with phylogenetic relation to the India-1/Asia-5 lineage previously identified in India. This is the first study to provide genomic data on CDV in Bangladesh and the first demonstration of a mobile laboratory setup as a powerful tool in rapid genomic surveillance and risk assessment for CDV in low resource regions.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Lanszki, Zsófia/0000-0003-3116-4633; Tóth, Gábor Endre/0000-0002-7201-9646; Kemenesi, Gábor/0000-0001-9775-3065} } @article{MTMT:34772955, title = {Endometrium development patterns and BMI groups among in vitro fertilization patients; prognostic aspects}, url = {https://m2.mtmt.hu/api/publication/34772955}, author = {Vedelek, Viktor and Petra, Bicskei and Mariann, Tábi and Biróné Lajkó, Noémi and Csaba, Ékes and Bereczki, Kristóf and Zsófia, Meixner-Csáti and Sinka, Rita and Vágvölgyi, Anna and Zádori, János}, doi = {10.3389/fendo.2024.1379109}, journal-iso = {FRONT ENDOCRINOL}, journal = {FRONTIERS IN ENDOCRINOLOGY}, volume = {15}, unique-id = {34772955}, issn = {1664-2392}, year = {2024}, eissn = {1664-2392}, orcid-numbers = {Sinka, Rita/0000-0003-4040-4184; Vágvölgyi, Anna/0000-0002-8810-700X} } @article{MTMT:34772438, title = {Mitochondrial Differentiation during Spermatogenesis: Lessons from Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/34772438}, author = {Vedelek, Viktor and Jankovics, Ferenc and Zádori, János and Sinka, Rita}, doi = {10.3390/ijms25073980}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {25}, unique-id = {34772438}, issn = {1661-6596}, abstract = {Numerous diseases can arise as a consequence of mitochondrial malfunction. Hence, there is a significant focus on studying the role of mitochondria in cancer, ageing, neurodegenerative diseases, and the field of developmental biology. Mitochondria could exist as discrete organelles in the cell; however, they have the ability to fuse, resulting in the formation of interconnected reticular structures. The dynamic changes between these forms correlate with mitochondrial function and mitochondrial health, and consequently, there is a significant scientific interest in uncovering the specific molecular constituents that govern these transitions. Moreover, the specialized mitochondria display a wide array of variable morphologies in their cristae formations. These inner mitochondrial structures are closely associated with the specific functions performed by the mitochondria. In multiple cases, the presence of mitochondrial dysfunction has been linked to male sterility, as it has been observed to cause a range of abnormal spermatogenesis and sperm phenotypes in different species. This review aims to elucidate the dynamic alterations and functions of mitochondria in germ cell development during the spermatogenesis of Drosophila melanogaster.}, year = {2024}, eissn = {1422-0067}, orcid-numbers = {Vedelek, Viktor/0000-0002-0420-8226; Sinka, Rita/0000-0003-4040-4184} } @{MTMT:34727182, title = {CRISPR-Cas9-based mutant library construction and characterization in Mucor lusitanicus}, url = {https://m2.mtmt.hu/api/publication/34727182}, author = {Abu Saleem, Tammam Khaliefeh Siliman and Molnár, Anna and Kovács, Vanda and Kiss, Karina and Rafael, Bence and SZEGEDI, Botond and Sinka, Rita and NÉMETH, Dóra and Homa, Mónika and Vágvölgyi, Csaba and Papp, Tamás and Szebenyi, Csilla and NÉMETH, Dóra}, booktitle = {Biotechnológiai Szakmai Nap Absztraktfüzet}, unique-id = {34727182}, year = {2024}, pages = {37-39}, orcid-numbers = {Sinka, Rita/0000-0003-4040-4184; Homa, Mónika/0000-0002-2724-4713; Vágvölgyi, Csaba/0000-0003-0009-7773; Papp, Tamás/0000-0001-8211-5431} } @article{MTMT:34498427, title = {White plague among the “forgotten people” from the Barbaricum of the Carpathian Basin–Cases with tuberculosis from the Sarmatian-period (3rd–4th centuries CE) archaeological site of Hódmezővásárhely–Kenyere-ér, Bereczki-tanya (Hungary)}, url = {https://m2.mtmt.hu/api/publication/34498427}, author = {Spekker, Olga and Kiss P., Attila and Kis, Luca and Király, Kitty and Varga, Sándor and Marcsik, Antónia and Schütz, Oszkár and Török, Tibor and Hunt, David R. and Tihanyi, Balázs}, doi = {10.1371/journal.pone.0294762}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {19}, unique-id = {34498427}, issn = {1932-6203}, abstract = {Tuberculosis (TB) is a bacterial infection that is well-known in the palaeopathological record because it can affect the skeleton and consequently leaves readily identifiable macroscopic alterations. Palaeopathological case studies provide invaluable information about the spatio-temporal distribution of TB in the past. This is true for those archaeological periods and geographical regions from when and where no or very few TB cases have been published until now–as in the Sarmatian period (1 st –5 th centuries CE) in the Barbaricum of the Carpathian Basin. The aim of our paper is to discuss five newly discovered TB cases ( HK199 , HK201 , HK225 , HK253 , and HK309 ) from the Sarmatian-period archaeological site of Hódmezővásárhely–Kenyere-ér, Bereczki-tanya (Csongrád-Csanád county, Hungary). Detailed macromorphological evaluation of the skeletons focused on the detection of bony changes likely associated with different forms of TB. In all five cases, the presence of endocranial alterations (especially TB-specific granular impressions) suggests that these individuals suffered from TB meningitis. Furthermore, the skeletal lesions observed in the spine and both hip joints of HK225 indicate that this juvenile also had multifocal osteoarticular TB. Thanks to the discovery of HK199 , HK201 , HK225 , HK253 , and HK309 , the number of TB cases known from the Sarmatian-period Carpathian Basin doubled, implying that the disease was likely more frequent in the Barbaricum than previously thought. Without the application of granular impressions, the diagnosis of TB could not have been established in these five cases. Thus, the identification of TB in these individuals highlights the importance of diagnostics development, especially the refinement of diagnostic criteria. Based on the above, the systematic macromorphological (re-)evaluation of osteoarchaeological series from the Sarmatian-period Carpathian Basin would be advantageous to provide a more accurate picture of how TB may have impacted the ancestral human communities of the Barbaricum.}, year = {2024}, eissn = {1932-6203}, orcid-numbers = {Spekker, Olga/0000-0002-0350-1906; Kis, Luca/0000-0002-1021-2854; Marcsik, Antónia/0000-0002-3121-4365; Schütz, Oszkár/0000-0001-5521-3044; Török, Tibor/0000-0002-2128-1126; Tihanyi, Balázs/0000-0001-5124-4468} } @CONFERENCE{MTMT:34684460, title = {Convergent evolution in glutamate dehydrogenase activity in Drosophila and human}, url = {https://m2.mtmt.hu/api/publication/34684460}, author = {Viktor, Vedelek and Balázs, Vedelek and Gabor, Juhasz and Lőrincz, Péter and Sinka, Rita}, booktitle = {27th European Drosophila Research Conference}, unique-id = {34684460}, year = {2023}, orcid-numbers = {Lőrincz, Péter/0000-0001-7374-667X; Sinka, Rita/0000-0003-4040-4184} } @CONFERENCE{MTMT:34684451, title = {Dual role for the orthologue of HECA, Headcase, in blood cell progenitor maintenance in the Drosophila lymph gland}, url = {https://m2.mtmt.hu/api/publication/34684451}, author = {Bayan, Kharrat and Nikolett, Virág and Erika, Gábor and Ildikó, Kristó and Aladár, Pettkó-Szandtner and Sinka, Rita and Ferenc, Jankovics and Viktor, Honti}, booktitle = {27th European Drosophila Research Conference}, unique-id = {34684451}, year = {2023}, orcid-numbers = {Sinka, Rita/0000-0003-4040-4184} } @CONFERENCE{MTMT:34684442, title = {Versatile ncMTOC organization during spermatogenesis}, url = {https://m2.mtmt.hu/api/publication/34684442}, author = {Sinka, Rita and Alzyoud, Elham and Dóra, Németh and Mónika, Krecsmarik and Viktor, Vedelek and Zsuzsánna, Réthi-Nagy and Zoltán, Lipinszki}, booktitle = {27th European Drosophila Research Conference}, unique-id = {34684442}, year = {2023}, orcid-numbers = {Sinka, Rita/0000-0003-4040-4184} } @inbook{MTMT:34680638, title = {A Drosophila kutatás története a Szegedi Tudományegyetem Genetika Tanszékén}, url = {https://m2.mtmt.hu/api/publication/34680638}, author = {Sinka, Rita}, booktitle = {Centenáriumi Tanulmánykötet}, unique-id = {34680638}, year = {2023}, pages = {149-163}, orcid-numbers = {Sinka, Rita/0000-0003-4040-4184} }