TY - JOUR AU - Nyeste, Krisztián József AU - Zulkipli , Nurfatin Binti AU - Uzochukwu, Ifeanyi Emmanuel AU - Somogyi, Dóra AU - Nagy, László AU - Czeglédi, István AU - Harangi, Sándor AU - Baranyai, Edina AU - Simon, Edina AU - Nagy, Sándor Alex AU - Velcheva, Iliana AU - Yancheva, Vesela AU - Antal, László TI - Assessment of trace and macroelement accumulation in cyprinid juveniles as bioindicators of aquatic pollution: effects of diets and habitat preferences JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-024-61986-4 UR - https://m2.mtmt.hu/api/publication/34863272 ID - 34863272 AB - Juveniles of three cyprinids with various diets and habitat preferences were collected from the Szamos River (Hungary) during a period of pollution in November 2013: the herbivorous, benthic nase ( Chondrostoma nasus ), the benthivorous, benthic barbel ( Barbus barbus ), and the omnivorous, pelagic chub ( Squalius cephalus ). Our study aimed to assess the accumulation of these elements across species with varying diets and habitat preferences, as well as their potential role in biomonitoring efforts. The Ca, K, Mg, Na, Cd, Cr, Cu, Fe, Mn, Pb, Sr, and Zn concentration was analyzed in muscle, gills, and liver using MP-AES. The muscle and gill concentrations of Cr, Cu, Fe, and Zn increased with trophic level. At the same time, several differences were found among the trace element patterns related to habitat preferences. The trace elements, including Cd, Pb, and Zn, which exceeded threshold concentrations in the water, exhibited higher accumulations mainly in the muscle and gills of the pelagic chub. Furthermore, the elevated concentrations of trace elements in sediments (Cr, Cu, Mn) demonstrated higher accumulation in the benthic nase and barbel. Our findings show habitat preference as a key factor in juvenile bioindicator capability, advocating for the simultaneous use of pelagic and benthic juveniles to assess water and sediment pollution status. LA - English DB - MTMT ER - TY - JOUR AU - Gyulai, István AU - Korponai, János AU - Wamugi, Sheila Mumbi A. AU - Jakab, Jázmin AU - Kawu, Umar Abba AU - Soltész, Andor Gergő AU - Karches, Tamás AU - Tumurtogoo, Uyanga TI - Cladocera and Geochemical Variables from Core Sediments Show Different Conditions of Hungarian Lakes JF - WATER J2 - WATER-SUI VL - 16 PY - 2024 IS - 9 SP - 1 EP - 12 PG - 12 SN - 2073-4441 DO - 10.3390/w16091310 UR - https://m2.mtmt.hu/api/publication/34853138 ID - 34853138 AB - Studies on the sediments of lakes with varying trophic status are of particular importance when considering changes in the natural environment. In this study, our objective was to examine subfossil remains of Cladocera species and the relationship between the sedimental Cladocera assemblages and geochemical variables during 11 years of sediment records from northern Hungarian lakes. To achieve this, we compared sedimental cladoceran communities and the geochemistry of the sediment layers among lakes. Among the studied lakes, one was an intermittent lake (KMT: the Kis-Morotva Lake) which dried out in 2012 but was subsequently naturally refilled in 2013 by groundwater affected by the high-water level of the River Tisza. The other type consisted of permanent lakes (SZA: the Szabolcs oxbow lake, TI: the Timár Morotva Lake) that never dried out. The results of the beta diversity analysis show that the deposition of Cladocera communities was similar among the sediment layers of lakes, while the abundance differences contributed significantly to replacement. Subsequently, core sediment samples of the three lakes were compared based on the remains of Cladocera communities and geochemical variables using Adonis (PERMANOVA). The core sediment samples indicate variations in Cladocera communities alongside disparities in geochemical variables across the same lakes. In conclusion, the significance of sediment cores containing the remains of the Cladocera community has grown significantly in the reconstruction of historical ecological and climatic changes. LA - English DB - MTMT ER - TY - GEN AU - Márton, Alexandra AU - Bíró, Vivien AU - Flipphi, Michel Johannes Anthonie AU - Fekete, Erzsébet AU - Karaffa, Levente TI - INSIGHTS INTO THE EVOLUTION AND MUTATIONS OF SECOND ALTERNATIVE OXIDASE GENES IN ASPERGILLACEAE CY - HUN-REN ATK Martonvásár, 4-5. April 2024. PY - 2024 UR - https://m2.mtmt.hu/api/publication/34847873 ID - 34847873 AB - Alternative oxidase (Aox) is a branched mitochondrial terminal oxidase, that bypasses Complex III and IV. Aox accepts electrons directly from ubiquinol and reduces oxygen to water without contributing to the proton gradient used for ATP synthesis. Aox is known to have various functions, including helping cells manage stress conditions, regulating cellular metabolism, and maintaining redox balance. In certain organisms, such as plants, it can play a role in preventing excessive reactive oxygen species production under stress conditions. Aox is nearly ubiquitous in fungi, yet the presence of multiple aox genes is uncommon. However, a second aox gene (aoxB) is present in some taxa of Aspergillaceae. Paralogous genes typically originate from duplication events and are passed down vertically. We offer evidence of four separate duplication events along the lineage that resulted in aox paralogues (aoxB) in contemporary Aspergillus and Penicillium taxa. In certain species, three aox genes are co-expressed, yet there are entire sections and series within Aspergillus that lose transient aoxB content. Within the subgenus Nidulantes, we have identified seven instances of independent aoxB gene loss and two instances of gain. The paralogous clades originate from widespread aoxA parent genes but never replace them, aoxA remains permanent across filamentous fungi. Within the database, Aspergillus niger strains possess six different alleles of the aoxB gene. Besides the wild type, we found five different mutations that caused errors in the gene product. A full-length AoxB is encoded in the acid producer ATCC 1015 strain. The investigation of alternative oxidase genes is crucial for a deeper understanding of citric acid production or clinical aspects. Fermentation occurs based on highly complex biochemical relationships, wherein the aox gene plays a crucial role. Understanding a gene well significantly contributes to strain development or possible clinical treatment. LA - English DB - MTMT ER - TY - CHAP AU - Bíró, Vivien AU - Márton, Alexandra AU - Fekete, Erzsébet AU - Karaffa, Levente TI - MANGANESE EFFECT ON CITRIC ACID PRODUCTION BY ASPERGILLUS NIGER: UNLOCKING AN EFFICIENCY BOOSTING HIDDEN KEY T2 - FIBOK 2024 6th National Conference of Young Biotechnologists PB - MTA Agrártudományok Osztálya, Mezőgazdasági Biotechnológiai Tudományos Bizottság CY - Budapest SN - 9786156448453 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34847840 ID - 34847840 AB - Achieving high yields of citric acid requires a unique combination of culture conditions, with the deficiency of manganese(II) ions in the growth medium being especially crucial. Concentrations exceeding 5 μg/L (= 5 ppb) result in a reduction of around 25% in the final citric acid yield. Because of its characteristics, this organic acid finds utility across a spectrum of industrial sectors, spanning from food and beverage to detergent and pharmaceutical industries. The predominant method for citric acid production involves large-scale industrial fermentations utilizing the filamentous fungus Aspergillus niger. When compared to alternative hosts, A. niger stands out for its ability to achieve remarkably high yields, with potential outputs reaching up to 95 kg of citric acid per 100 kg of sugar. Technical-scale production of citric acid predominantly uses stainless steel tank fermenters. However, glass bioreactors, commonly used for process development, also incorporate stainless steel components, where manganese serves as a crucial alloying element. Our study reveals that manganese(II) ions leach from these bioreactors into the growth medium during citric acid fermentation. This leaching phenomenon leads to alterations in fungal physiology and morphology, resulting in a significant decrease in citric acid yields. The extent of manganese(II) ion leaching depends on factors such as fermentation duration, the acidity of the culture broth, and the sterilization method employed. Moreover, CexA is the main citrate exporter of A. niger. The citric acid production was examined in overexpression mutant strains of cexA under manganese deficiency and sufficient conditions. This leads to citric acid accumulation even in the presence of high manganese(II) ion concentrations. Additionally, the impact of CexA on fungal morphology was elucidated through microscopic analysis. LA - English DB - MTMT ER - TY - GEN AU - Bíró, Vivien AU - Márton, Alexandra AU - Fekete, Erzsébet AU - Karaffa, Levente TI - MANGANESE EFFECT ON CITRIC ACID PRODUCTION BY ASPERGILLUS NIGER: UNLOCKING AN EFFICIENCY BOOSTING HIDDEN KEY CY - HUN-REN ATK Martonvásár, 4-5. April 2024. PY - 2024 UR - https://m2.mtmt.hu/api/publication/34847827 ID - 34847827 AB - Achieving high yields of citric acid requires a unique combination of culture conditions, with the deficiency of manganese(II) ions in the growth medium being especially crucial. Concentrations exceeding 5 μg/L (= 5 ppb) result in a reduction of around 25% in the final citric acid yield. Because of its characteristics, this organic acid finds utility across a spectrum of industrial sectors, spanning from food and beverage to detergent and pharmaceutical industries. The predominant method for citric acid production involves large-scale industrial fermentations utilizing the filamentous fungus Aspergillus niger. When compared to alternative hosts, A. niger stands out for its ability to achieve remarkably high yields, with potential outputs reaching up to 95 kg of citric acid per 100 kg of sugar. Technical-scale production of citric acid predominantly uses stainless steel tank fermenters. However, glass bioreactors, commonly used for process development, also incorporate stainless steel components, where manganese serves as a crucial alloying element. Our study reveals that manganese(II) ions leach from these bioreactors into the growth medium during citric acid fermentation. This leaching phenomenon leads to alterations in fungal physiology and morphology, resulting in a significant decrease in citric acid yields. The extent of manganese(II) ion leaching depends on factors such as fermentation duration, the acidity of the culture broth, and the sterilization method employed. Moreover, CexA is the main citrate exporter of A. niger. The citric acid production was examined in overexpression mutant strains of cexA under manganese deficiency and sufficient conditions. This leads to citric acid accumulation even in the presence of high manganese(II) ion concentrations. Additionally, the impact of CexA on fungal morphology was elucidated through microscopic analysis. LA - English DB - MTMT ER - TY - CHAP AU - Márton, Alexandra AU - Bíró, Vivien AU - Flipphi, Michel Johannes Anthonie AU - Fekete, Erzsébet AU - Karaffa, Levente TI - INSIGHTS INTO THE EVOLUTION AND MUTATIONS OF SECOND ALTERNATIVE OXIDASE GENES IN ASPERGILLACEAE T2 - FIBOK 2024 6th National Conference of Young Biotechnologists PB - MTA Agrártudományok Osztálya, Mezőgazdasági Biotechnológiai Tudományos Bizottság CY - Budapest SN - 9786156448453 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34847686 ID - 34847686 AB - Alternative oxidase (Aox) is a branched mitochondrial terminal oxidase, that bypasses Complex III and IV. Aox accepts electrons directly from ubiquinol and reduces oxygen to water without contributing to the proton gradient used for ATP synthesis. Aox is known to have various functions, including helping cells manage stress conditions, regulating cellular metabolism, and maintaining redox balance. In certain organisms, such as plants, it can play a role in preventing excessive reactive oxygen species production under stress conditions. Aox is nearly ubiquitous in fungi, yet the presence of multiple aox genes is uncommon. However, a second aox gene (aoxB) is present in some taxa of Aspergillaceae. Paralogous genes typically originate from duplication events and are passed down vertically. We offer evidence of four separate duplication events along the lineage that resulted in aox paralogues (aoxB) in contemporary Aspergillus and Penicillium taxa. In certain species, three aox genes are co-expressed, yet there are entire sections and series within Aspergillus that lose transient aoxB content. Within the subgenus Nidulantes, we have identified seven instances of independent aoxB gene loss and two instances of gain. The paralogous clades originate from widespread aoxA parent genes but never replace them, aoxA remains permanent across filamentous fungi. Within the database, Aspergillus niger strains possess six different alleles of the aoxB gene. Besides the wild type, we found five different mutations that caused errors in the gene product. A full-length AoxB is encoded in the acid producer ATCC 1015 strain. The investigation of alternative oxidase genes is crucial for a deeper understanding of citric acid production or clinical aspects. Fermentation occurs based on highly complex biochemical relationships, wherein the aox gene plays a crucial role. Understanding a gene well significantly contributes to strain development or possible clinical treatment. LA - English DB - MTMT ER - TY - GEN AU - Márton, Alexandra AU - Bíró, Vivien AU - Flipphi, Michel Johannes Anthonie AU - Fekete, Erzsébet AU - Karaffa, Levente TI - INSIGHTS INTO THE EVOLUTION AND MUTATIONS OF SECOND ALTERNATIVE OXIDASE GENES IN ASPERGILLACEAE CY - HUN-REN ATK Martonvásár, 4-5. April 2024. PY - 2024 UR - https://m2.mtmt.hu/api/publication/34847654 ID - 34847654 AB - Alternative oxidase (Aox) is a branched mitochondrial terminal oxidase, that bypasses Complex III and IV. Aox accepts electrons directly from ubiquinol and reduces oxygen to water without contributing to the proton gradient used for ATP synthesis. Aox is known to have various functions, including helping cells manage stress conditions, regulating cellular metabolism, and maintaining redox balance. In certain organisms, such as plants, it can play a role in preventing excessive reactive oxygen species production under stress conditions. Aox is nearly ubiquitous in fungi, yet the presence of multiple aox genes is uncommon. However, a second aox gene (aoxB) is present in some taxa of Aspergillaceae. Paralogous genes typically originate from duplication events and are passed down vertically. We offer evidence of four separate duplication events along the lineage that resulted in aox paralogues (aoxB) in contemporary Aspergillus and Penicillium taxa. In certain species, three aox genes are co-expressed, yet there are entire sections and series within Aspergillus that lose transient aoxB content. Within the subgenus Nidulantes, we have identified seven instances of independent aoxB gene loss and two instances of gain. The paralogous clades originate from widespread aoxA parent genes but never replace them, aoxA remains permanent across filamentous fungi. Within the database, Aspergillus niger strains possess six different alleles of the aoxB gene. Besides the wild type, we found five different mutations that caused errors in the gene product. A full-length AoxB is encoded in the acid producer ATCC 1015 strain. The investigation of alternative oxidase genes is crucial for a deeper understanding of citric acid production or clinical aspects. Fermentation occurs based on highly complex biochemical relationships, wherein the aox gene plays a crucial role. Understanding a gene well significantly contributes to strain development or possible clinical treatment. LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Roland Tibor AU - Bán, Miklós AU - Barta, Zoltán TI - Mowing wet meadows reduces the health of their snail communities JF - PEERJ J2 - PEERJ VL - 12 PY - 2024 PG - 16 SN - 2167-8359 DO - 10.7717/peerj.16783 UR - https://m2.mtmt.hu/api/publication/34841708 ID - 34841708 AB - Wet meadows harbor rich biodiversity, making them pivotal ecosystems worldwide. These habitats are commonly used for grazing or hay production for livestock. However, regular mowing can influence these habitats, potentially leading to significant repercussions for the animals residing within them. In order to investigate the effects of land management practices, we conducted an experimental study to compare snail communities in mowed and unmowed wet meadows in northern Hungary. We found that overall, mowing decreases snail populations, as well as species richness and diversity. Thus, our results suggest that routine mowing of wet meadows is deleterious to their snail communities. Based on these results, we suggest that designated patches of meadows that are regularly managed should be left uncultivated in their natural state. These patches can serve as potential colonization sites, facilitating the restoration of the entire meadow’s ecological balance. LA - English DB - MTMT ER - TY - CONF AU - Karaffa, Levente AU - Flipphi, Michel Johannes Anthonie AU - Márton, Alexandra AU - Bíró, Vivien AU - Bakondi-Kovács, István AU - Ág-Rácz, Viktória AU - Ág, Norbert AU - Fekete, Erzsébet TI - Second Alternative Oxidase Genes in Aspergillaceae: Genesis, Loss and Mutations T2 - The 20th International Aspergillus Meeting,Asperfest20 Program and Abstract Book PY - 2024 SP - 26 EP - 27 PG - 2 UR - https://m2.mtmt.hu/api/publication/34751176 ID - 34751176 AB - Alternative oxidase (Aox) is a terminal oxidase in branched mitochondrial electron transport that provides a non-electrogenic alternative to canonical cytochrome-mediated electron flow, bypassing the proton- pumping complexes III and IV. The consequence of the direct transfer of electrons from ubiquinol to oxygen without concomitant build up of proton motive force is the uncoupling of ATP synthesis via oxidative phosporylation from NADH reoxidation, to allow carbon catabolism to continue unabated even when ATP demand is low or when non-carbon nutrients become limiting. Thus, Aox plays an important role in the energetics of overflow metabolism-based bioprocesses such as Aspergillus niger citric acid fermentation and Aspergillus terreus itaconate production. Aox (aoxA gene) is near ubiquitous in the fungal kingdom, but coexistence of multiple aox genes is rare. However, a second aox gene (aoxB) is present in some taxa of Aspergillaceae. Paralogous genes generally originate from duplication and inherit vertically; we provide evidence for four independent duplication events at different points in evolution that resulted in aoxB paralogs in contemporary Aspergilli and Penicillia. The paralog in A. niger has a different origin than the paralog in A. terreus, while a third independently formed paralog is found in A. wentii. All paralogous clades arise from original aoxA parent genes but never replace them. Few species have accumulated three co-expressed aox genes. Therefore, loss of once acquired paralogs co-determines contemporary aox gene content in individual species. For instance, section Fumigati has lost all its transient paralogs. In the subgenus Nidulantes, we identified seven independent occasions of aoxB gene loss and two gains. In A. calidoustus, both more ancient aoxB paralogs present in the last common ancestor of the subgenus have been substituted by two other aoxB genes of completely distinct origins. We found that the paralogous aoxB gene in some 75 genome-sequenced A. niger strains features variation at a level not detected for the ubiquitous aoxA gene. Five mutations were identified that plausibly affect transcription, function, or terminally modify the gene product. A full-length AoxB is encoded in the acid producer ATCC 1015. Hence, the A. niger sensu stricto complex can be subdivided into six taxa according to the resident aoxB allele. To date, confident separation could only be accomplished after comparative analyses of whole genome sequences. LA - English DB - MTMT ER - TY - GEN AU - Karaffa, Levente AU - Flipphi, Michel Johannes Anthonie AU - Márton, Alexandra AU - Bíró, Vivien AU - Bakondi-Kovács, István AU - Ág-Rácz, Viktória AU - Ág, Norbert AU - Fekete, Erzsébet TI - Second Alternative Oxidase Genes in Aspergillaceae: Genesis, Loss and Mutations CY - Asperfest20 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34751163 ID - 34751163 AB - Alternative oxidase (Aox) is a terminal oxidase in branched mitochondrial electron transport that provides a non-electrogenic alternative to canonical cytochrome-mediated electron flow, bypassing the proton- pumping complexes III and IV. The consequence of the direct transfer of electrons from ubiquinol to oxygen without concomitant build up of proton motive force is the uncoupling of ATP synthesis via oxidative phosporylation from NADH reoxidation, to allow carbon catabolism to continue unabated even when ATP demand is low or when non-carbon nutrients become limiting. Thus, Aox plays an important role in the energetics of overflow metabolism-based bioprocesses such as Aspergillus niger citric acid fermentation and Aspergillus terreus itaconate production. Aox (aoxA gene) is near ubiquitous in the fungal kingdom, but coexistence of multiple aox genes is rare. However, a second aox gene (aoxB) is present in some taxa of Aspergillaceae. Paralogous genes generally originate from duplication and inherit vertically; we provide evidence for four independent duplication events at different points in evolution that resulted in aoxB paralogs in contemporary Aspergilli and Penicillia. The paralog in A. niger has a different origin than the paralog in A. terreus, while a third independently formed paralog is found in A. wentii. All paralogous clades arise from original aoxA parent genes but never replace them. Few species have accumulated three co-expressed aox genes. Therefore, loss of once acquired paralogs co-determines contemporary aox gene content in individual species. For instance, section Fumigati has lost all its transient paralogs. In the subgenus Nidulantes, we identified seven independent occasions of aoxB gene loss and two gains. In A. calidoustus, both more ancient aoxB paralogs present in the last common ancestor of the subgenus have been substituted by two other aoxB genes of completely distinct origins. We found that the paralogous aoxB gene in some 75 genome-sequenced A. niger strains features variation at a level not detected for the ubiquitous aoxA gene. Five mutations were identified that plausibly affect transcription, function, or terminally modify the gene product. A full-length AoxB is encoded in the acid producer ATCC 1015. Hence, the A. niger sensu stricto complex can be subdivided into six taxa according to the resident aoxB allele. To date, confident separation could only be accomplished after comparative analyses of whole genome sequences. LA - English DB - MTMT ER -