TY - JOUR AU - Bognár, Zsófia AU - Mosshammer, Maria AU - Brodersen, Kasper E. AU - Bollati, Elena AU - Gyurcsányi, Ervin Róbert AU - Kuhl, Michael TI - Multiparameter Sensing of Oxygen and pH at Biological Interfaces via Hyperspectral Imaging of Luminescent Sensor Nanoparticles JF - ACS SENSORS J2 - ACS SENSORS VL - 9 PY - 2024 IS - 4 SP - 1763 EP - 1774 PG - 12 SN - 2379-3694 DO - 10.1021/acssensors.3c01941 UR - https://m2.mtmt.hu/api/publication/34824724 ID - 34824724 N1 - Funding Agency and Grant Number: Gordon and Betty Moore Foundation [TKP2021]; Ministry of Innovation and Technology of Hungary from the National Research, Development, and Innovation Fund [DFF-8022-00301B & DFF-8021-00308B]; Independent Research Fund Denmark [GBMF9206, 10.37807/GBMF9206]; Gordon and Betty Moore Foundation [00028156, VIL50371]; Villum Foundation [DNRF146]; DTU VISION from the Danish National Research Foundation (Center for Visualizing Catalytic Processes); European Commission [860125]; European Union Funding text: We thank Sofie L. Jakobsen for her excellent technical assistance. This study was supported by the Ministry of Innovation and Technology of Hungary from the National Research, Development, and Innovation Fund (R.E.G. BME-EGA-02 under TKP2021), the Independent Research Fund Denmark (M.K.; DFF-8022-00301B & DFF-8021-00308B), an investigator award from the Gordon and Betty Moore Foundation (M.K.; grant no. GBMF9206; 10.37807/GBMF9206), grants from the Villum Foundation (K.E.B.; Villum Experiment: 00028156, and M.M.; Villum Experiment: VIL50371), DTU VISION from the Danish National Research Foundation (Center for Visualizing Catalytic Processes, DNRF146 (Z.B.)), an Erasmus+ Traineeship financed by the European Commission (Z.B.) and a grant from the European Union's Horizon 2020 research and innovation programme (M.K.; Marie Sk & lstrok;odowska-Curie grant agreement No 860125). AB - Chemical dynamics in biological samples are seldom stand-alone processes but represent the outcome of complicated cascades of interlinked reaction chains. In order to understand these processes and how they correlate, it is important to monitor several parameters simultaneously at high spatial and temporal resolution. Hyperspectral imaging is a promising tool for this, as it provides broad-range spectral information in each pixel, enabling the use of multiple luminescent indicator dyes, while simultaneously providing information on sample structures and optical properties. In this study, we first characterized pH- and O-2-sensitive indicator dyes incorporated in different polymer matrices as optical sensor nanoparticles to provide a library for (hyperspectral) chemical imaging. We then demonstrate the successful combination of a pH-sensitive indicator dye (HPTS(DHA)(3)), an O-2-sensitive indicator dye (PtTPTBPF), and two reference dyes (perylene and TFPP), incorporated in polymer nanoparticles for multiparameter chemical imaging of complex natural samples such as green algal biofilms (Chlorella sorokiniana) and seagrass leaves (Zostera marina) with high background fluorescence. We discuss the system-specific challenges and limitations of our approach and further optimization possibilities. Our study illustrates how multiparameter chemical imaging with hyperspectral read-out can now be applied on natural samples, enabling the alignment of several chemical parameters to sample structures. LA - English DB - MTMT ER - TY - JOUR AU - Zhang, Xiaorong AU - Yarman, Aysu AU - Kovács, Norbert AU - Bognár, Zsófia AU - Gyurcsányi, Ervin Róbert AU - Bier, Frank F. AU - Scheller, Frieder W. TI - Specific features of epitope-MIPs and whole-protein MIPs as illustrated for AFP and RBD of SARS-CoV-2 JF - MICROCHIMICA ACTA J2 - MICROCHIM ACTA VL - 191 PY - 2024 IS - 5 PG - 9 SN - 0026-3672 DO - 10.1007/s00604-024-06325-0 UR - https://m2.mtmt.hu/api/publication/34785838 ID - 34785838 N1 - Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Str. 24-25, Potsdam, 14476, Germany Molecular Biotechnology, Faculty of Science, Turkish-German University, Sahinkaya Cad. Beykoz, Istanbul, 34820, Turkey BME “Lendület” Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, 1111, Hungary HUN-REN-BME Computation Driven Chemistry Research Group, Műegyetem rkp. 3, Budapest, 1111, Hungary Export Date: 12 April 2024 CODEN: MIACA Correspondence Address: Zhang, X.; Institute of Biochemistry and Biology, Karl-Liebknecht Str. 24-25, Germany; email: xiaorong.zhang.1@uni-potsdam.de AB - Molecularly imprinted polymer (MIP) nanofilms for alpha-fetoprotein (AFP) and the receptor binding domain (RBD) of the spike protein of SARS-CoV-2 using either a peptide (epitope-MIP) or the whole protein (protein-MIP) as the template were prepared by electropolymerization of scopoletin. Conducting atomic force microscopy revealed after template removal and electrochemical deposition of gold a larger surface density of imprinted cavities for the epitope-imprinted polymers than when using the whole protein as template. However, comparable affinities towards the respective target protein (AFP and RBD) were obtained for both types of MIPs as expressed by the KD values in the lower nanomolar range. On the other hand, while the cross reactivity of both protein-MIPs towards human serum albumin (HSA) amounts to around 50% in the saturation region, the nonspecific binding to the respective epitope-MIPs is as low as that for the non-imprinted polymer (NIP). This effect might be caused by the different sizes of the imprinted cavities. Thus, in addition to the lower costs the reduced nonspecific binding is an advantage of epitope-imprinted polymers for the recognition of proteins. Graphical Abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024. LA - English DB - MTMT ER - TY - JOUR AU - Ahmed, Marwa AU - Hessz, Dóra AU - Gyarmati, Benjámin Sándor AU - Pancsics, Mirko AU - Kovács, Norbert AU - Gyurcsányi, Ervin Róbert AU - Kubinyi, Miklós AU - Horváth, Viola TI - A generic approach based on long-lifetime fluorophores for the assessment of protein binding to polymer nanoparticles by fluorescence anisotropy JF - NANOSCALE J2 - NANOSCALE VL - 16 PY - 2024 IS - 7 SP - 3659 EP - 3667 PG - 9 SN - 2040-3364 DO - 10.1039/d3nr02460a UR - https://m2.mtmt.hu/api/publication/34569163 ID - 34569163 N1 - Funding Agency and Grant Number: Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund under the TKP2021 funding scheme; National Research, Development and Innovation (NRDI) Office, Hungary [BME-EGA-02]; Hungarian Academy of Sciences; Stipendium Hungaricum Scholarship; [FK 138029] Funding text: The research reported in this paper is part of project no. BME-EGA-02, implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021 funding scheme. Further support was provided by the National Research, Development and Innovation (NRDI) Office, Hungary via grant FK 138029. B. Gy. and M. A. A. acknowledge the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences and the Stipendium Hungaricum Scholarship, respectively. AB - Quantitation of protein-nanoparticle interactions is essential for the investigation of the protein corona around NPs in vivo and when using synthetic polymer nanoparticles as affinity reagents for selective protein recognition in vitro. Here, a method based on steady-state fluorescence anisotropy measurement is presented as a novel, separation-free tool for the assessment of protein-nanoparticle interactions. For this purpose, a long-lifetime luminescent Ru-complex is used for protein labelling, which exhibits low anisotropy when conjugated to the protein but displays high anisotropy when the proteins are bound to the much larger polymer nanoparticles. As a proof of concept, the interaction of lysozyme with poly(N-isopropylacrylamide-co-N-tert-butylacrylamide-co-acrylic acid) nanoparticles is studied, and fluorescence anisotropy measurements are used to establish the binding kinetics, binding isotherm and a competitive binding assay. Using long-lifetime fluorophores as protein labels, protein-nanoparticle interactions can be monitored through anisotropy change. Besides gaining thermodynamic and kinetic information on the binding process, competitive protein assays can be set up. LA - English DB - MTMT ER - TY - JOUR AU - Bognár, Zsófia AU - Supala, Eszter AU - Yarman, Aysu AU - Zhang, Xiaorong AU - Bier, Frank F. AU - Scheller, Frieder W. AU - Gyurcsányi, Ervin Róbert TI - Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein (vol 13, pg 1263, 2022) JF - CHEMICAL SCIENCE J2 - CHEM SCI VL - 14 PY - 2023 IS - 36 SP - 9980 EP - 9980 PG - 1 SN - 2041-6520 DO - 10.1039/d3sc90170j UR - https://m2.mtmt.hu/api/publication/34192911 ID - 34192911 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Fund; [TKP2021] Funding text: The research reported in this paper is part of Project BME-EGA-02, implemented with support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021 funding scheme. AB - Correction for 'Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein' by Zsofia Bognar et al., Chem. Sci., 2022, 13, 1263-1269, https://doi.org/10.1039/D1SC04502D. LA - English DB - MTMT ER - TY - JOUR AU - Solymosi, Gergely Tamás AU - Gyurcsányi, Ervin Róbert TI - Understanding the potentiometric response of cation-permselective hydrophilic nanopore-based electrodes in the low ion concentration range JF - ELECTROCHEMISTRY COMMUNICATIONS J2 - ELECTROCHEM COMMUN VL - 153 PY - 2023 SN - 1388-2481 DO - 10.1016/j.elecom.2023.107540 UR - https://m2.mtmt.hu/api/publication/34085469 ID - 34085469 N1 - Export Date: 3 August 2023 CODEN: ECCMF Correspondence Address: Gyurcsányi, R.E.; BME Lendület Chemical Nanosensors Research Group, Műegyetem rkp. 3, H-1111, Hungary; email: robert.gyurcsanyi@vbk.bme.hu LA - English DB - MTMT ER - TY - JOUR AU - Szalay, Péter ED - Gyurcsányi, Ervin Róbert / Interviewed person TI - A kémiai érzékelés határainak feszegetése JF - MAGYAR KÉMIKUSOK LAPJA J2 - MAGY KEM LAP VL - 78 PY - 2023 IS - 4 SP - 108 EP - 110 PG - 3 SN - 0025-0163 UR - https://m2.mtmt.hu/api/publication/34069356 ID - 34069356 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Bognár, Zsófia AU - Kozma, József AU - Kovács, Norbert AU - Gyurcsányi, Ervin Róbert TI - Novel functional monomer for the electrochemical synthesis of highly affine epitope-imprinted polymers JF - ELECTROANALYSIS J2 - ELECTROANAL VL - 35 PY - 2023 IS - 6 SN - 1040-0397 DO - 10.1002/elan.202300025 UR - https://m2.mtmt.hu/api/publication/33727389 ID - 33727389 N1 - Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary BME Lendület Chemical Nanosensors Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary ELKH-BME Computation Driven Chemistry Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary CODEN: ELANE Correspondence Address: Gyurcsányi, R.E.; Department of Inorganic and Analytical Chemistry, Műegyetem rkp. 3, Hungary; email: gyurcsanyi.robert@vbk.bme.hu LA - English DB - MTMT ER - TY - JOUR AU - Kozma, József AU - Papp, Soma AU - Gyurcsányi, Ervin Róbert TI - Highly hydrophobic TEMPO-functionalized conducting copolymers for solid-contact ion-selective electrodes JF - BIOELECTROCHEMISTRY J2 - BIOELECTROCHEMISTRY VL - 150 PY - 2023 SN - 1567-5394 DO - 10.1016/j.bioelechem.2022.108352 UR - https://m2.mtmt.hu/api/publication/33453038 ID - 33453038 N1 - Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [BME-NVA-02, BME-EGA-02]; New National Excellence Program of the Ministry for Innovation and Technology [UNKP-21-3] Funding text: The research reported in this paper is part of project no. BME-EGA-02 and BME-NVA-02, implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021 funding scheme. Further support was received from National Research, Development and Innovation Fund through UNKP-21-3 New National Excellence Program of the Ministry for Innovation and Technology. The authors would like to thank to Dr. Lajos Hofler for the inspiring discussion on the margin of GITT experiments. AB - Solid-contact ion-selective electrodes (SCISEs) emerged as the best electrode embodiment for miniaturized, wearable and disposable sensors for ion/electrolyte measurements in body fluids. The commercialization of inexpensive single-use "calibration-free" electrodes requires large scale manufacturing of electrodes with reproducible calibration parameters, e.g. E0. This is perhaps the most important shortcoming of SCISEs, beside the many advantages over their conventional liquid-contact counterparts. However, adjusting the E0 value for optimal potential stability is challenging for all state-of-the-art solid-contact materials, which may combine several types of transducing mechanism (e.g. capacitive and redox materials or their combination) for enhanced potential stability and analytical performance. Therefore, here we introduce for the first time the galvanostatic intermittent titration technique (GITT) to determine the best preadjusment potential. The proof of concept is shown for a novel type of solid-contact based on the copolymerization of 3,4-ethylenedioxythiophene with perfluorinated alkyl side chain (EDOTF) and (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl modified 3,4-ethylenedioxythiophene (EDOT-TEMPO). Such materials that are compliant with local electrodeposition and provide multiple functionalities, i.e. high hydrophobicity by the perfluorinated alkyl side chain, electron-to-ion transduction by the conducting polymer (EDOT) backbone and the confinement of well-defined redox couple (TEMPO), are expected to prevail as solid-contacts. LA - English DB - MTMT ER - TY - JOUR AU - Zhang, Xiaorong AU - Waffo, Armel T. AU - Yarman, Aysu AU - Kovács, Norbert AU - Bognár, Zsófia AU - Wollenberger, Ulla AU - El-Sherbiny, Ibrahim M. AU - Hassan, Rabeay Y. A. AU - Bier, Frank F. AU - Gyurcsányi, Ervin Róbert AU - Zebger, Ingo AU - Scheller, Frieder W. TI - How an ACE2 mimicking epitope-MIP nanofilm recognizes template-related peptides and the receptor binding domain of SARS-CoV-2 JF - NANOSCALE J2 - NANOSCALE VL - 14 PY - 2022 IS - 48 SP - 18106 EP - 18114 PG - 9 SN - 2040-3364 DO - 10.1039/D2NR03898F UR - https://m2.mtmt.hu/api/publication/33452970 ID - 33452970 N1 - Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Str. 24-25, Potsdam, 14476, Germany Institut für Chemie, PC 14 Technische Universität Berlin, Straße des 17. Juni 135, Berlin, 10623, Germany Molecular Biotechnology, Faculty of Science, Turkish-German University, Sahinkaya Cad, 86, Beykoz, Istanbul, 34820, Turkey Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary ELKH-BME Computation Driven Chemistry Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary Nanoscience Program, University of Science and Technology (UST), Center for Materials Science (CMS), Zewail City of Science and Technology, Giza, 12578, Egypt Cited By :1 Export Date: 5 April 2023 Correspondence Address: Gyurcsányi, R.E.; Department of Inorganic and Analytical Chemistry, Műegyetem rkp. 3, Hungary; email: gyurcsanyi.robert@vbk.bme.hu Correspondence Address: Scheller, F.W.; Institute of Biochemistry and Biology, Karl-Liebknecht Str. 24-25, Germany; email: fschell@uni-potsdam.de AB - We developed original methods to confirm the liberation of the imprinted binding cavities by electrochemical template removal and identified the amino acid motif of the template which is determinant for the affinity of the epitope-imprinted polymer. LA - English DB - MTMT ER - TY - JOUR AU - Kozma, József AU - Papp, Soma AU - Gyurcsányi, Ervin Róbert TI - TEMPO-Functionalized Carbon Nanotubes for Solid-Contact Ion-Selective Electrodes with Largely Improved Potential Reproducibility and Stability JF - ANALYTICAL CHEMISTRY J2 - ANAL CHEM VL - 94 PY - 2022 IS - 23 SP - 8249 EP - 8257 PG - 9 SN - 0003-2700 DO - 10.1021/acs.analchem.2c00395 UR - https://m2.mtmt.hu/api/publication/32897099 ID - 32897099 N1 - Export Date: 21 June 2022 CODEN: ANCHA Correspondence Address: Gyurcsányi, R.E.; Department of Inorganic and Analytical Chemistry, Müegyetem rkp. 3, Hungary; email: gyurcsanyi.robert@vbk.bme.hu LA - English DB - MTMT ER -