TY - JOUR AU - Szűcs, Zsolt AU - Cziáky, Zoltán AU - Volánszki, László AU - Máthé, Csaba AU - Vasas, Gábor AU - Gonda, Sándor TI - Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 13 PY - 2024 IS - 10 PG - 19 SN - 2223-7747 DO - 10.3390/plants13101288 UR - https://m2.mtmt.hu/api/publication/34843399 ID - 34843399 AB - Medicinal plant tissue cultures are potential sources of bioactive compounds. In this study, we report the chemical characterization of the callus cultures of three medicinal Tilia spp. (Tilia cordata, Tilia vulgaris and Tilia tomentosa), along with the comparison to bracts and flowers of the same species. Our aim was to show that calli of Tilia spp. are good alternatives to the calli of T. americana for the production of polyphenols and are better sources of a subset of polyphenolic metabolites, compared to the original organs. Calli were initiated from young bracts and grown on woody plant medium containing 1 mg L−1 2,4-D and 0.1 mg L−1 BAP. For chemical characterization, a quality-controlled untargeted metabolomics approach and the quantification of several bioactive compounds was performed with the use of LC-ESI-MS/MS. While bracts and flowers contained flavonoid glycosides (astragalin, isoquercitrin) as major polyphenols, calli of all species contained catechins, coumarins (fraxin, esculin and scopoletin) and flavane aglyca. T. tomentosa calli contained 5397 µg g DW−1 catechin, 201 µg g DW−1 esculin, 218 µg g DW−1 taxifolin and 273 µg g DW−1 eriodictyol, while calli from other species contained lower amounts. T. cordata and T. tomentosa flowers were rich in isoquercitrin, containing 8134 and 6385 µg g DW−1, respectively. The currently tested species contained many of the bioactive metabolites described from T. americana. The production of catechin was shown to be comparable to the most efficient tissue cultures reported. Flowers and bracts contained flavonoid glycosides, including tiliroside, resembling bioactive fractions of T. americana. In addition, untargeted metabolomics has shown fingerprint-like differences among species, highlighting possible chemotaxonomic and quality control applications, especially for bracts. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Loránd AU - Bertalan, László AU - Szabó, Gergely AU - Grigorszky, István AU - Somlyai, Imre AU - Dévai, György AU - Nagy, Sándor Alex AU - Holb, Imre AU - Szabó, Szilárd TI - Aquatic vegetation mapping with UAS-cameras considering phenotypes JF - ECOLOGICAL INFORMATICS J2 - ECOL INFORM PY - 2024 SN - 1574-9541 DO - 10.1016/j.ecoinf.2024.102624 UR - https://m2.mtmt.hu/api/publication/34839516 ID - 34839516 AB - Aquatic vegetation species at the genus level in an oxbow lake were identified in Hungary based on a multispectral Uncrewed Aerial System (UAS ) survey within an elongated oxbow lake area of the Tisza River under continental climate. Seven and 13 classes were discriminated using three different classification methods (Support Vector Machine [SVM], Random Forest [RF] , and Multivariate Adaptive Regression Splines [MARS]) using different input data in ten combinations: original spectral bands , spectral indices, Digital Surface Model (DSM) , and Haralick texture indices. We achieved a high (97.1%) overall accuracies (OAs) by applying the SVM classifier, but the RF performed only <1% worse, as it was represented in the first places of the classification rank before the MARS. The highest classification accuracies (>84% OA) were obtained using the most important variables derived by the Recursive Feature Elimination (RFE) method . The best classification required DSM as an input variable. The poorest classification performance belonged to the model that used only texture indices or spectral indices. On the class level, Stratoites aloides exhibit the lowest degree of separability compared to the other classes. Accordingly, we recommend using supplementary input data for the classifications beside s the original spectral bands, for example , DSM, spectral , and texture indices, as these variables significantly improve the classification accuracies in the proper combinations of the input variables . LA - English DB - MTMT ER - TY - JOUR AU - Máthé, Csaba AU - Bóka, Károly AU - Kónya, Zoltán AU - Erdődi, Ferenc AU - Vasas, Gábor AU - Freytag, Csongor AU - Garda, Tamás TI - Microcystin-LR, a cyanotoxin, modulates division of higher plant chloroplasts through protein phosphatase inhibition and affects cyanobacterial division JF - CHEMOSPHERE J2 - CHEMOSPHERE VL - 358 PY - 2024 PG - 11 SN - 0045-6535 DO - 10.1016/j.chemosphere.2024.142125 UR - https://m2.mtmt.hu/api/publication/34836717 ID - 34836717 N1 - Plant Cell and Developmental Biology Research Group, Department of Botany, Institute of Biology and Ecology, Faculty of Science and Technology, University of Debrecen, Egyetem ter 1, Debrecen, H-4032, Hungary Department of Plant Anatomy, Institute of Biology, Faculty of Science, ELTE Eötvös Loránd University, Pázmány P. s. 1/c, Budapest, H-1117, Hungary Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Egyetem ter 1, Debrecen, H-4032, Hungary Plant and Algal Natural Product Research Group, Department of Botany, Institute of Biology and Ecology, Faculty of Science and Technology, University of Debrecen, Egyetem ter 1, Debrecen, H-4032, Hungary Balaton Limnological Research Institute- HUN-REN, Klebelsberg str. 3, Tihany, H-8237, Hungary One Health Institute, Faculty of Health Sciences, University of Debrecen, Nagyerdei krt. 98, Debrecen, H-4032, Hungary Export Date: 8 May 2024 CODEN: CMSHA Correspondence Address: Máthé, C.; Plant Cell and Developmental Biology Research Group, Egyetem ter 1, Hungary; email: mathe.csaba@science.unideb.hu LA - English DB - MTMT ER - TY - JOUR AU - Gálné Remenyik, Judit AU - Csige, László AU - Dávid, Péter AU - Fauszt, Péter AU - Szilágyi-Rácz, Anna Anita AU - Szőllősi, Erzsébet AU - Bacsó, Zsófia Réka AU - Szepsy Jnr, István AU - Molnár, Krisztina AU - Rácz, Csaba AU - Fidler, Gábor AU - Kállai, Zoltán AU - Stündl, László AU - Dobos, Attila Csaba AU - Paholcsek, Melinda TI - Exploring the interplay between the core microbiota, physicochemical factors, agrobiochemical cycles in the soil of the historic tokaj mád wine region JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 4 PG - 24 SN - 1932-6203 DO - 10.1371/journal.pone.0300563 UR - https://m2.mtmt.hu/api/publication/34832088 ID - 34832088 AB - A Hungarian survey of Tokaj-Mád vineyards was conducted. Shotgun metabarcoding was applied to decipher the microbial-terroir. The results of 60 soil samples showed that there were three dominant fungal phyla, Ascomycota 66.36% ± 15.26%, Basidiomycota 18.78% ± 14.90%, Mucoromycota 11.89% ± 8.99%, representing 97% of operational taxonomic units (OTUs). Mutual interactions between microbiota diversity and soil physicochemical parameters were revealed. Principal component analysis showed descriptive clustering patterns of microbial taxonomy and resistance gene profiles in the case of the four historic vineyards (Szent Tamás, Király, Betsek, Nyúlászó). Linear discriminant analysis effect size was performed, revealing pronounced shifts in community taxonomy based on soil physicochemical properties. Twelve clades exhibited the most significant shifts (LDA > 4.0), including the phyla Verrucomicrobia , Bacteroidetes , Chloroflexi , and Rokubacteria , the classes Acidobacteria , Deltaproteobacteria , Gemmatimonadetes , and Betaproteobacteria , the order Sphingomonadales , Hypomicrobiales , as well as the family Sphingomonadaceae and the genus Sphingomonas . Three out of the four historic vineyards exhibited the highest occurrences of the bacterial genus Bradyrhizobium , known for its positive influence on plant development and physiology through the secretion of steroid phytohormones. During ripening, the taxonomical composition of the soil fungal microbiota clustered into distinct groups depending on altitude, differences that were not reflected in bacteriomes. Network analyses were performed to unravel changes in fungal interactiomes when comparing postveraison and preharvest samples. In addition to the arbuscular mycorrhiza Glomeraceae , the families Mycosphaerellacae and Rhyzopodaceae and the class Agaricomycetes were found to have important roles in maintaining soil microbial community resilience. Functional metagenomics showed that the soil Na content stimulated several of the microbiota-related agrobiogeochemical cycles, such as nitrogen and sulphur metabolism; steroid, bisphenol, toluene, dioxin and atrazine degradation and the synthesis of folate. LA - English DB - MTMT ER - TY - JOUR AU - Bodnár , Veronika AU - Antal, Károly AU - de Vries, Ronald P. AU - Pócsi, István AU - Emri, Tamás TI - Aspergillus nidulans gfdB, Encoding the Hyperosmotic Stress Protein Glycerol-3-phosphate Dehydrogenase, Disrupts Osmoadaptation in Aspergillus wentii JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 10 PY - 2024 IS - 4 PG - 23 SN - 2309-608X DO - 10.3390/jof10040291 UR - https://m2.mtmt.hu/api/publication/34802432 ID - 34802432 AB - The genome of the osmophilic Aspergillus wentii, unlike that of the osmotolerant Aspergillus nidulans, contains only the gfdA, but not the gfdB, glycerol 3-phosphate dehydrogenase gene. Here, we studied transcriptomic changes of A. nidulans (reference strain and ΔgfdB gene deletion mutant) and A. wentii (reference strain and An-gfdB expressing mutant) elicited by high osmolarity. A. nidulans showed a canonic hyperosmotic stress response characterized by the upregulation of the trehalose and glycerol metabolism genes (including gfdB), as well as the genes of the high-osmolarity glycerol (HOG) map kinase pathway. The deletion of gfdB caused only negligible alterations in the transcriptome, suggesting that the glycerol metabolism was flexible enough to compensate for the missing GfdB activity in this species. A. wentii responded differently to increased osmolarity than did A. nidulans, e.g., the bulk upregulation of the glycerol and trehalose metabolism genes, along with the HOG pathway genes, was not detected. The expression of An-gfdB in A. wentii did not abolish osmophily, but it reduced growth and caused much bigger alterations in the transcriptome than did the missing gfdB gene in A. nidulans. Flexible glycerol metabolism and hence, two differently regulated gfd genes, may be more beneficial for osmotolerant (living under changing osmolarity) than for osmophilic (living under constantly high osmolarity) species. LA - English DB - MTMT ER - TY - CHAP AU - Aszalósné Balogh, Rebeka AU - Lőkös, László AU - Adorján, Balázs AU - Freytag, Csongor AU - Mészáros, Ilona AU - Oláh, Viktor AU - Szűcs, Péter AU - Erzberger, Peter AU - Farkas, Edit AU - Matus, Gábor ED - Buró, Botond ED - Molnár, Mihály TI - Városi lapostetők mint kriptogám élőhelyek T2 - XIX. Kárpát-medencei Környezettudományi Konferencia. Absztrakt füzet PB - MTA Atommagkutató Intézet CY - Debrecen SN - 9789638321602 T3 - Kárpát-Medencei Környezettudományi Konferencia, ISSN 1842-9815 PY - 2024 SP - 104 EP - 105 PG - 2 UR - https://m2.mtmt.hu/api/publication/34801279 ID - 34801279 LA - English DB - MTMT ER - TY - JOUR AU - Triest, Ludwig AU - Van Rossum, Fabienne AU - Sramkó, Gábor AU - Sierens, Tim AU - Volkova, Polina TI - Over the hills and far away: phylogeography and demographic migration history of a dispersal-restricted primrose (Primula vulgaris) JF - FRONTIERS IN ECOLOGY AND EVOLUTION J2 - FRONT ECOL EVOL VL - 12 PY - 2024 PG - 21 SN - 2296-701X DO - 10.3389/fevo.2024.1333726 UR - https://m2.mtmt.hu/api/publication/34796911 ID - 34796911 AB - Introduction: Quaternary glaciations, in particular the last glacial maximum (LGM), have shaped the contemporary distribution of many species. In Europe, survival of temperate species during range contractions was mainly associated with refugia in Mediterranean peninsulas, allowing south to north recolonization. Additionally, the Ponto-Caspian refugium provided an east to west migration route. Methods: Here, we investigated genetic diversity and structure of the dispersal-restricted Primula vulgaris Huds., a temperate herb of supposed Caucasian origin, using 12 highly polymorphic nuclear microsatellite loci to trace the colonization of this species in three European regions. We studied 38 populations, namely an eastern mountainous region (Caucasus), a central region (Carpathian Mountains and Dinaric Alps) and a western lowland region (North European Plain). The role of an eastern refugium of the species was tested by performing Approximate Bayesian computation analyses to elucidate its demographic and phylogeographic history, detecting bottleneck and expansion events, and estimating effective (ancestral and current) population sizes and time of divergence. Results and discussion: We found the eastern and central regions featuring the highest allelic richness and genetic differentiation being strong between and within regions. Stepwise-like mutation processes (RST > FST), together with isolation-by-distance patterns, contributed to genetic differentiation. Demographic event models showed that P. vulgaris experienced bottlenecks as well as expansions across its range during the Quaternary glacial cycles, with the last expansion about 6,000, 4,000, and 2,000 generations ago for eastern, central, and western populations, respectively. The best supported origin model pointed to the Caucasus population being closest to an ancestral situation, and from where central and western populations diverged subsequently. Divergence time of the Caucasus populations from an ancestral lineage referred to the Upper Pleistocene (Middle Weichselian). Divergence of Carpathian populations, including a Lower Danube valley corridor, was estimated during and across the LGM (Late Weichselian), confirming central populations as potential secondary cryptic refugium. Western populations recently diverged during the Holocene, most probably in the Atlanticum phase (7,000–3,900 years ago). Within regions, genetic structure was also shaped by latitude, longitude, or altitudinal migration, and for western lowlands, by recent bottleneck and genetic drift processes. LA - English DB - MTMT ER - TY - CHAP AU - Gebrehaweria Kidane, Reda AU - Ndunguru, Sawadi Fransisco AU - Csernus, Brigitta AU - Szabó, Csaba AU - Czeglédi, Levente AU - Lendvai, Ádám Zoltán ED - Hajdú, Péter TI - Expression of nutrient sensing genes mediate the effect of dietary unpredictability in Japanese quails T2 - II. Magyar Agrártudományi Doktoranduszok Szimpóziuma 2024 PB - Doktoranduszok Országos Szövetsége (DOSZ) CY - Budapest SN - 9786156457431 PY - 2024 SP - 27 UR - https://m2.mtmt.hu/api/publication/34794891 ID - 34794891 LA - English DB - MTMT ER - TY - JOUR AU - Gebrehaweria Kidane, Reda AU - Ndunguru, Sawadi Fransisco AU - Csernus, Brigitta AU - Knop, Renáta AU - Lugata, James Kachungwa AU - Szabó, Csaba AU - Czeglédi, Levente AU - Lendvai, Ádám Zoltán TI - Dietary restriction reveals sex-specific expression of the mTOR pathway genes in Japanese quails JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-024-58487-9 UR - https://m2.mtmt.hu/api/publication/34786100 ID - 34786100 AB - Limited resources affect an organism’s physiology through the conserved metabolic pathway, the mechanistic target of rapamycin (mTOR). Males and females often react differently to nutritional limitation, but whether it leads to differential mTOR pathway expression remains unknown. Recently, we found that dietary restriction (DR) induced significant changes in the expression of mTOR pathway genes in female Japanese quails ( Coturnix japonica ). We simultaneously exposed 32 male and female Japanese quails to either 20%, 30%, 40% restriction or ad libitum feeding for 14 days and determined the expression of six key genes of the mTOR pathway in the liver to investigate sex differences in the expression patterns. We found that DR significantly reduced body mass, albeit the effect was milder in males compared to females. We observed sex-specific liver gene expression. DR downregulated mTOR expression more in females than in males. Under moderate DR, ATG9A and RPS6K1 expressions were increased more in males than in females. Like females, body mass in males was correlated positively with mTOR and IGF1, but negatively with ATG9A and RS6K1 expressions. Our findings highlight that sexes may cope with nutritional deficits differently and emphasise the importance of considering sexual differences in studies of dietary restriction. LA - English DB - MTMT ER - TY - JOUR AU - Gebrehaweria Kidane, Reda AU - Ndunguru, Sawadi Fransisco AU - Csernus, Brigitta AU - Tóthné Gulyás, Gabriella AU - Knop, Renáta AU - Szabó, Csaba AU - Czeglédi, Levente AU - Lendvai, Ádám Zoltán TI - Dietary restriction and life-history trade-offs: insights into mTOR pathway regulation and reproductive investment in Japanese quails JF - JOURNAL OF EXPERIMENTAL BIOLOGY J2 - J EXP BIOL PY - 2024 SN - 0022-0949 DO - 10.1242/jeb.247064 UR - https://m2.mtmt.hu/api/publication/34785047 ID - 34785047 AB - Resources are needed for growth, reproduction and survival, and organisms must trade-off limited resources among competing processes. Nutritional availability in organisms is sensed and monitored by nutrient-sensing pathways that can trigger physiological changes or alter gene expression. Previous studies have proposed that one such signalling pathway, the mechanistic target of rapamycin (mTOR), underpins a form of adaptive plasticity when individuals encounter constraints in their energy budget. Despite the fundamental importance of this process in evolutionary biology, how nutritional limitation is regulated through the expression of genes governing this pathway and its consequential effects on fitness remains understudied, particularly in birds. We used dietary restriction to simulate resource depletion and examined its effects on body mass, reproduction and gene expression in Japanese quails (Coturnix japonica). Quails were subjected to feeding 20%, 30%, 40% restriction levels or ad libitum for two weeks. All restricted groups exhibited reduced body mass, whereas reductions in the number and mass of eggs were observed only under more severe restrictions. Additionally, dietary restriction led to decreased expression of mTOR and insulin-like growth factor 1 (IGF1), whereas the ribosomal protein S6 kinase 1 (RPS6K1) and autophagy-related genes (ATG9A and ATG5) were upregulated. The pattern in which mTOR respond to restriction was similar to what has been seen in body mass. Regardless of the treatment, proportionally higher reproductive investment was associated with individual variation in mTOR expression. These findings reveal the connection between dietary intake and the expression of mTOR and related genes in this pathway. LA - English DB - MTMT ER -