TY - JOUR AU - Bunda, Szilvia AU - Kálmán-Szabó, Ibolya AU - Lihi, Norbert AU - Képes, Zita AU - Szikra, Dezső AU - Péliné Szabó, Judit AU - Timári, István AU - Szücs, Dániel AU - May, Nóra Veronika AU - Papp, Gábor Csaba AU - Trencsényi, György AU - Kálmán, Ferenc Krisztián TI - Diagnosis of Melanoma with 61 Cu-Labeled PET Tracer JF - JOURNAL OF MEDICINAL CHEMISTRY J2 - J MED CHEM PY - 2024 SN - 0022-2623 DO - 10.1021/acs.jmedchem.4c00479 UR - https://m2.mtmt.hu/api/publication/34861986 ID - 34861986 LA - English DB - MTMT ER - TY - JOUR AU - Mobasheri, Ali AU - Matta, Csaba AU - Giles, Wayne AU - Choi, Heonsik AU - Ivanavicius, Stefan TI - The interplay between inflammatory mediators and mechanotransduction is mediated by ion channels in articular chondrocytes: Functional consequences in osteoarthritis JF - PHYSICS OF LIFE REVIEWS J2 - PHYS LIFE REV VL - 49 PY - 2024 SP - 123 EP - 126 PG - 4 SN - 1571-0645 DO - 10.1016/j.plrev.2024.04.008 UR - https://m2.mtmt.hu/api/publication/34859982 ID - 34859982 LA - English DB - MTMT ER - TY - JOUR AU - Dócs, Klaudia AU - Balázs, Anita AU - Papp, Ildikó AU - Szűcs, Péter AU - Hegyi, Zoltán TI - Reactive spinal glia convert 2-AG to prostaglandins to drive aberrant astroglial calcium signaling JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 18 PY - 2024 PG - 17 SN - 1662-5102 DO - 10.3389/fncel.2024.1382465 UR - https://m2.mtmt.hu/api/publication/34857224 ID - 34857224 N1 - Edited by: Renato Socodato, University of Porto, Portugal Reviewed by: - Robert Kraft, Leipzig University, Germany - Francesco Ferrini, University of Turin, Italy - Marcello Melone, Marche Polytechnic University, Italy AB - The endogenous cannabinoid 2-arachidonoylglycerol (2-AG) influences neurotransmission in the central nervous system mainly by activating type 1 cannabinoid receptor (CB1). Following its release, 2-AG is broken down by hydrolases to yield arachidonic acid, which may subsequently be metabolized by cyclooxygenase-2 (COX-2). COX-2 converts arachidonic acid and also 2-AG into prostanoids, well-known inflammatory and pro-nociceptive mediators. Here, using immunohistochemical and biochemical methods and pharmacological manipulations, we found that reactive spinal astrocytes and microglia increase the expression of COX-2 and the production of prostaglandin E2 when exposed to 2-AG. Both 2-AG and PGE2 evoke calcium transients in spinal astrocytes, but PGE2 showed 30% more efficacy and 55 times more potency than 2-AG. Unstimulated spinal dorsal horn astrocytes responded to 2-AG with calcium transients mainly through the activation of CB1. 2-AG induced exaggerated calcium transients in reactive astrocytes, but this increase in the frequency and area under the curve of calcium signals was only partially dependent on CB1. Instead, aberrant calcium transients were almost completely abolished by COX-2 inhibition. Our results suggest that both reactive spinal astrocytes and microglia perform an endocannabinoid-prostanoid switch to produce PGE2 at the expense of 2-AG. PGE2 in turn is responsible for the induction of aberrant astroglial calcium signals which, together with PGE2 production may play role in the development and maintenance of spinal neuroinflammation-associated disturbances such as central sensitization. LA - English DB - MTMT ER - TY - JOUR AU - Obajed Al-Ali, Omar AU - Pfliegler, György AU - Magyari, Ferenc AU - Borics, Fanni AU - Pinczés, László Imre AU - Illés, Árpád AU - Brúgós, Boglárka Csilla TI - A Case Report of Hyperhemolytic Syndrome in Sickle Cell Disease, with a Special Focus on Avoiding the Use of Transfusions JF - THALASSEMIA REPORTS J2 - THALASS REP VL - 14 PY - 2024 IS - 1 SP - 18 EP - 25 PG - 8 SN - 2039-4357 DO - 10.3390/thalassrep14010003 UR - https://m2.mtmt.hu/api/publication/34856127 ID - 34856127 AB - In patients with sickle cell disease (SCD), transfusions pose risks like delayed hemolytic transfusion reaction (DHTR) and hyperhemolytic syndrome (HHS). We present the case of a 61-year-old Nigerian male patient with SCD, developing hyperhemolytic syndrome (HHS) post-orthopedic surgery due to alloimmunization from blood transfusions. Surgery induced massive hemorrhage, requiring RBC transfusions. Postoperatively, he developed HHS with jaundice, hemoglobinuria, and fever. Despite additional transfusions, his condition worsened, leading to hematological consultation on postoperative day +9. Laboratory findings showed positive DAT and multiple alloantibodies. The diagnosis of HHS was established and treatment involved high-dose methylprednisolone, intravenous immunoglobulin (IVIG), and erythropoietin. The patient was discharged on postoperative day +24 with stable hemoglobin levels, tapering doses of methylprednisolone, and continuous administration of hydroxyurea prescribed. HHS pathogenesis involves extensive intravascular hemolysis, exacerbated by alloimmunization. Diagnostic challenges and therapy selection complexity underscore the need for cautious transfusion strategies in HHS, reserving them for hemodynamic instability or hypoxia. This case highlights promptly recognizing and managing HHS in SCD for improved outcomes and avoiding unnecessary transfusions. LA - English DB - MTMT ER - TY - JOUR AU - Enyedi, Enikő E. AU - Petukhov, Pavel A. AU - Kozuch, Alexander J. AU - Dudek, Steven M. AU - Toth, Attila AU - Fagyas, Miklós AU - Danilov, Sergei M. TI - ACE Phenotyping in Human Blood and Tissues: Revelation of ACE Outliers and Sex Differences in ACE Sialylation JF - BIOMEDICINES J2 - BIOMEDICINES VL - 12 PY - 2024 IS - 5 SP - 940 SN - 2227-9059 DO - 10.3390/biomedicines12050940 UR - https://m2.mtmt.hu/api/publication/34855859 ID - 34855859 AB - Angiotensin-converting enzyme (ACE) metabolizes a number of important peptides participating in blood pressure regulation and vascular remodeling. Elevated ACE expression in tissues (which is generally reflected by blood ACE levels) is associated with an increased risk of cardiovascular diseases. Elevated blood ACE is also a marker for granulomatous diseases. Decreased blood ACE activity is becoming a new risk factor for Alzheimer’s disease. We applied our novel approach—ACE phenotyping—to characterize pairs of tissues (lung, heart, lymph nodes) and serum ACE in 50 patients. ACE phenotyping includes (1) measurement of ACE activity with two substrates (ZPHL and HHL); (2) calculation of the ratio of hydrolysis of these substrates (ZPHL/HHL ratio); (3) determination of ACE immunoreactive protein levels using mAbs to ACE; and (4) ACE conformation with a set of mAbs to ACE. The ACE phenotyping approach in screening format with special attention to outliers, combined with analysis of sequencing data, allowed us to identify patient with a unique ACE phenotype related to decreased ability of inhibition of ACE activity by albumin, likely due to competition with high CCL18 in this patient for binding to ACE. We also confirmed recently discovered gender differences in sialylation of some glycosylation sites of ACE. ACE phenotyping is a promising new approach for the identification of ACE phenotype outliers with potential clinical significance, making it useful for screening in a personalized medicine approach. LA - English DB - MTMT ER - TY - JOUR AU - Szűcs, Gabriella AU - Szekanecz, Zoltán AU - Szamosi, Szilvia TI - Can we define difficult-to-treat systemic sclerosis? JF - EXPERT REVIEW OF CLINICAL IMMUNOLOGY J2 - EXPERT REV CLIN IMMUNOL PY - 2024 SP - 1 EP - 17 PG - 17 SN - 1744-666X DO - 10.1080/1744666X.2024.2352450 UR - https://m2.mtmt.hu/api/publication/34855342 ID - 34855342 LA - English DB - MTMT ER - TY - JOUR AU - Serban, Norbert AU - Kupás, Dávid AU - Hajdu, András AU - Török, Péter AU - Harangi, Balázs TI - Distinguishing the Uterine Artery, the Ureter, and Nerves in Laparoscopic Surgical Images Using Ensembles of Binary Semantic Segmentation Networks JF - SENSORS J2 - SENSORS-BASEL VL - 24 PY - 2024 IS - 9 SP - 1 EP - 12 PG - 12 SN - 1424-8220 DO - 10.3390/s24092926 UR - https://m2.mtmt.hu/api/publication/34850523 ID - 34850523 AB - Performing a minimally invasive surgery comes with a significant advantage regarding rehabilitating the patient after the operation. But it also causes difficulties, mainly for the surgeon or expert who performs the surgical intervention, since only visual information is available and they cannot use their tactile senses during keyhole surgeries. This is the case with laparoscopic hysterectomy since some organs are also difficult to distinguish based on visual information, making laparoscope-based hysterectomy challenging. In this paper, we propose a solution based on semantic segmentation, which can create pixel-accurate predictions of surgical images and differentiate the uterine arteries, ureters, and nerves. We trained three binary semantic segmentation models based on the U-Net architecture with the EfficientNet-b3 encoder; then, we developed two ensemble techniques that enhanced the segmentation performance. Our pixel-wise ensemble examines the segmentation map of the binary networks on the lowest level of pixels. The other algorithm developed is a region-based ensemble technique that takes this examination to a higher level and makes the ensemble based on every connected component detected by the binary segmentation networks. We also introduced and trained a classic multi-class semantic segmentation model as a reference and compared it to the ensemble-based approaches. We used 586 manually annotated images from 38 surgical videos for this research and published this dataset. LA - English DB - MTMT ER - TY - JOUR AU - Csigó, Katalin AU - Münnich, Ákos AU - Molnár, Judit TI - The importance of examining early maladaptive schemas in the diagnosis and treatment of obsessive-compulsive disorder JF - FRONTIERS IN PSYCHIATRY J2 - FRONT PSYCHIATRY VL - 15 PY - 2024 PG - 10 SN - 1664-0640 DO - 10.3389/fpsyt.2024.1360127 UR - https://m2.mtmt.hu/api/publication/34849539 ID - 34849539 LA - English DB - MTMT ER - TY - JOUR AU - De Gasperi, Rita AU - Csernoch, László AU - Dienes, Beatrix AU - Gönczi, Mónika AU - Chakrabarty, Jayanta K. AU - Goeta, Shahar AU - Aslan, Abdurrahman AU - Toro, Carlos A. AU - Karasik, David AU - Brown, Lewis M. AU - Brotto, Marco AU - Cardozo, Christopher P. TI - Septin 7 interacts with Numb to preserve sarcomere structural organization and muscle contractile function JF - ELIFE J2 - ELIFE VL - 12 PY - 2024 PG - 18 SN - 2050-084X DO - 10.7554/eLife.89424.4 UR - https://m2.mtmt.hu/api/publication/34846822 ID - 34846822 AB - Here, we investigated mechanisms by which aging-related reductions of the levels of Numb in skeletal muscle fibers contribute to loss of muscle strength and power, two critical features of sarcopenia. Numb is an adaptor protein best known for its critical roles in development including asymmetric cell division, cell-type specification and termination of intracellular signaling. Numb expression is reduced in old humans and mice. We previously showed that, in mouse skeletal muscle fibers, Numb is localized to sarcomeres where it is concentrated near triads; conditional inactivation of Numb and a closely related protein Numb-like (NumbL) in mouse myofibers caused weakness, disorganization of sarcomeres and smaller mitochondria with impaired function. Here, we found that a single knockout of Numb in myofibers causes reduction in tetanic force comparable to a double Numb, NumbL knockout. We found by proteomics analysis of protein complexes isolated from C2C12 myotubes by immunoprecipitation using antibodies against Numb, that Septin 7 is a potential Numb binding partner. Septin 7 is a member of the family of GTP-binding proteins that organize into filaments, sheets and rings, and is considered part of the cytoskeleton. Immunofluorescence evaluation revealed a partial overlap of staining for Numb and Septin 7 in myofibers. Conditional, inducible knockouts of Numb led to disorganization of Septin 7 staining in myofibers. These findings indicate that Septin 7 is a Numb binding partner and suggest that interactions between Numb and Septin 7 are critical for structural organization of the sarcomere and muscle contractile function. LA - English DB - MTMT ER - TY - JOUR AU - Szekanecz, Zoltán AU - Szamosi, Szilvia AU - Benkő, Szilvia AU - Szűcs, Gabriella TI - Monogénesen öröklődő és szerzett autoinflammatoricus betegségek JF - ORVOSI HETILAP J2 - ORV HETIL VL - 165 PY - 2024 IS - 18 SP - 683 EP - 697 PG - 15 SN - 0030-6002 DO - 10.1556/650.2024.33038 UR - https://m2.mtmt.hu/api/publication/34845388 ID - 34845388 AB - Az autoinflammatio a természetes (innate) immunitás zavara, mely veleszületett, monogénes vagy szerzett lehet. A monogénes autoinflammatoricus kórképek közé tartoznak az inflammasomopathiák, az actinopathiák, az endoplazmatikus reticulum stresszt okozó mutációk, az NFκB-hez társult betegségek, az interferonopathiák, az endogén antagonisták génjeinek mutációi és a DADA2. A szerzett autoinflammatoricus betegségek közé számos gyulladásos reumatológiai kórképet, bél-, bőr- és csontbetegséget, valamint egyéb kórképeket (például VEXAS, IgG4-gyel társult betegség, recurrens pericarditis, 2-es típusú diabetes, interstitialis tüdőbetegség) sorolhatunk. Ebben az összefoglalóban áttekintjük az autoinflammatio koncepcióját és főbb mechanizmusait, a legfontosabb monogénes és szerzett autoinflammatoricus kórképeket, az immundeficientiák autoinflammatióban játszott szerepét, valamint a szóba jövő terápiás lehetőségeket. Autoinflammation is a disorder of natural (innate) immunity, which can be monogenic, or acquired. Monogenic autoinflammatory diseases include inflammasomopathies, actinopathies, endoplasmic reticulum stress mutations, NFκB-associated diseases, interferonopathies, mutations in endogenous antagonist genes, and DADA2. Acquired autoinflammatory diseases include numerous inflammatory rheumatological diseases, intestinal, skin and bone diseases, as well as other conditions (e.g., VEXAS, IgG4-related disease, recurrent pericarditis, type 2 diabetes, interstitial lung disease). In this summary, we review the concept and main mechanisms of autoinflammation, the most important monogenic and acquired autoinflammatory conditions, the role of inborn errors of immunity in autoinflammation, as well as the possible therapeutic options. LA - Hungarian DB - MTMT ER -