TY - JOUR AU - Toàn, Ngô Minh AU - Vágner, Adrienn AU - Nagy, Gábor AU - Ország, Gábor AU - Nagy, Tamás AU - Csikos, Csaba AU - Váradi, Balázs AU - Sajtos, Gergő Zoltán AU - Kapus, István AU - Szoboszlai, Zoltán AU - Szikra, Dezső AU - Trencsényi, György AU - Tircsó, Gyula AU - Garai, Ildikó TI - [52Mn]Mn-BPPA-Trastuzumab: A Promising HER2-Specific PET Radiotracer JF - JOURNAL OF MEDICINAL CHEMISTRY J2 - J MED CHEM VL - 67 PY - 2024 SP - 1 SN - 0022-2623 DO - 10.1021/acs.jmedchem.4c00344 UR - https://m2.mtmt.hu/api/publication/34831627 ID - 34831627 AB - This study aimed to develop a novel radiotracer using trastuzumab and the long-lived [52Mn]Mn isotope for HER2-targeted therapy selection and monitoring. A new Mn(II) chelator, BPPA, synthesized from a rigid bispyclen platform possessing a picolinate pendant arm, formed a stable and inert Mn(II) complex with favorable relaxation properties. BPPA was converted into a bifunctional chelator (BFC), conjugated to trastuzumab, and labeled with [52Mn]Mn isotope. In comparison to DOTA-GA-trastuzumab, the BPPA-trastuzumab conjugate exhibits a labeling efficiency with [52Mn]Mn approximately 2 orders of magnitude higher. In female CB17 SCID mice bearing 4T1 (HER2−) and MDA-MB-HER2+ (HER2+) xenografts, [52Mn]Mn-BPPA-trastuzumab demonstrated superior uptake in HER2+ cells on day 3, with a 3–4 fold difference observed on day 7. Overall, the hexadentate BPPA chelator proves to be exceptional in binding Mn(II). Upon coupling with trastuzumab as a BFC ligand, it becomes an excellent imaging probe for HER2-positive tumors. [52Mn]Mn-BPPA-trastuzumab enables an extended imaging time window and earlier detection of HER2-positive tumors with superior tumor-to-background contrast. LA - English DB - MTMT ER - TY - JOUR AU - Szekanecz, Zoltán AU - Kacsándi, Dorottya AU - Soós, Boglárka TI - A rheumatoid arthritis etiopatogenezise - újdonságok JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 16 PY - 2024 IS - 1 SP - 4 EP - 14 PG - 11 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/34831051 ID - 34831051 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szekanecz, Zoltán TI - A JAK-gátlók alkalmazásának jövőbeli lehetőségei krónikus gyulladásos kórképekben JF - ORVOSTOVÁBBKÉPZŐ SZEMLE J2 - ORVOSTOVÁBBKÉPZŐ SZLE PY - 2024 SN - 1218-2583 UR - https://m2.mtmt.hu/api/publication/34829474 ID - 34829474 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Ameri, Pietro AU - Mercurio, Valentina AU - Pollesello, Piero AU - Anker, Markus S. AU - Backs, Johannes AU - Bayes‐Genis, Antoni AU - Borlaug, Barry A. AU - Burkhoff, Daniel AU - Caravita, Sergio AU - Chan, Stephen Y. AU - de Man, Frances AU - Giannakoulas, George AU - González, Aránzazu AU - Guazzi, Marco AU - Hassoun, Paul M. AU - Hemnes, Anna R. AU - Maack, Cristoph AU - Madden, Brendan AU - Melenovsky, Vojtech AU - Müller, Oliver J. AU - Papp, Zoltán AU - Pullamsetti, Soni Savai AU - Rainer, Peter P. AU - Redfield, Margaret M. AU - Rich, Stuart AU - Schiattarella, Gabriele G. AU - Skaara, Hall AU - Stellos, Kostantinos AU - Tedford, Ryan J. AU - Thum, Thomas AU - Vachiery, Jean Luc AU - van der Meer, Peter AU - Van Linthout, Sophie AU - Pruszczyk, Piotr AU - Seferovic, Petar AU - Coats, Andrew J.S. AU - Metra, Marco AU - Rosano, Giuseppe AU - Rosenkranz, Stephan AU - Tocchetti, Carlo Gabriele TI - A roadmap for therapeutic discovery in pulmonary hypertension associated with left heart failure. A scientific statement of the Heart Failure Association ( HFA ) of the ESC and the ESC Working Group on Pulmonary Circulation & Right Ventricular Function JF - EUROPEAN JOURNAL OF HEART FAILURE J2 - EUR J HEART FAIL PY - 2024 SN - 1388-9842 DO - 10.1002/ejhf.3236 UR - https://m2.mtmt.hu/api/publication/34827495 ID - 34827495 AB - Pulmonary hypertension (PH) associated with left heart failure (LHF) (PH‐LHF) is one of the most common causes of PH. It directly contributes to symptoms and reduced functional capacity and negatively affects right heart function, ultimately leading to a poor prognosis. There are no specific treatments for PH‐LHF, despite the high number of drugs tested so far. This scientific document addresses the main knowledge gaps in PH‐LHF with emphasis on pathophysiology and clinical trials. Key identified issues include better understanding of the role of pulmonary venous versus arteriolar remodelling, multidimensional phenotyping to recognize patient subgroups positioned to respond to different therapies, and conduct of rigorous pre‐clinical studies combining small and large animal models. Advancements in these areas are expected to better inform the design of clinical trials and extend treatment options beyond those effective in pulmonary arterial hypertension. Enrichment strategies, endpoint assessments, and thorough haemodynamic studies, both at rest and during exercise, are proposed to play primary roles to optimize early‐stage development of candidate therapies for PH‐LHF. LA - English DB - MTMT ER - TY - JOUR AU - Horváth, Ádám AU - Steib, Anita AU - Nehr-Majoros, Andrea Kinga AU - Kántás, Boglárka AU - Király, Ágnes AU - Racskó, Márk AU - Tóth, Balázs István AU - Szánti-Pintér, Eszter AU - Kudová, Eva AU - Skodáné Földes, Rita AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Anti-Nociceptive Effects of Sphingomyelinase and Methyl-Beta-Cyclodextrin in the Icilin-Induced Mouse Pain Model JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 PG - 13 SN - 1661-6596 DO - 10.3390/ijms25094637 UR - https://m2.mtmt.hu/api/publication/34824919 ID - 34824919 AB - The thermo- and pain-sensitive Transient Receptor Potential Melastatin 3 and 8 (TRPM3 and TRPM8) ion channels are functionally associated in the lipid rafts of the plasma membrane. We have already described that cholesterol and sphingomyelin depletion, or inhibition of sphingolipid biosynthesis decreased the TRPM8 but not the TRPM3 channel opening on cultured sensory neurons. We aimed to test the effects of lipid raft disruptors on channel activation on TRPM3- and TRPM8-expressing HEK293T cells in vitro, as well as their potential analgesic actions in TRPM3 and TRPM8 channel activation involving acute pain models in mice. CHO cell viability was examined after lipid raft disruptor treatments and their effects on channel activation on channel expressing HEK293T cells by measurement of cytoplasmic Ca2+ concentration were monitored. The effects of treatments were investigated in Pregnenolone-Sulphate-CIM-0216-evoked and icilin-induced acute nocifensive pain models in mice. Cholesterol depletion decreased CHO cell viability. Sphingomyelinase and methyl-beta-cyclodextrin reduced the duration of icilin-evoked nocifensive behavior, while lipid raft disruptors did not inhibit the activity of recombinant TRPM3 and TRPM8. We conclude that depletion of sphingomyelin or cholesterol from rafts can modulate the function of native TRPM8 receptors. Furthermore, sphingolipid cleavage provided superiority over cholesterol depletion, and this method can open novel possibilities in the management of different pain conditions. LA - English DB - MTMT ER - TY - JOUR AU - Bäck, Magnus AU - Topouchian, Jirar AU - Labat, Carlos AU - Gautier, Sylvie AU - Blacher, Jacques AU - Cwynar, Marcin AU - de la Sierra, Alejandro AU - Páll, Dénes AU - Duarte, Kevin AU - Fantin, Francesco AU - Farkas, Katalin AU - Garcia-Ortiz, Luis AU - Hakobyan, Zoya AU - Jankowski, Piotr AU - Jelakovic, Ana AU - Kotsani, Marina AU - Konradi, Alexandra AU - Mikhailova, Oksana AU - Mintale, Iveta AU - Plunde, Oscar AU - Ramos, Rafael AU - Rogoza, Anatoly AU - Sirenko, Yuriy AU - Tasic, Nebojsa AU - Rudyk, Iurii AU - Urazalina, Saule AU - Wohlfahrt, Peter AU - Zelveian, Parounak AU - Asmar, Roland AU - Benetos, Athanase TI - Cardio-ankle vascular index for predicting cardiovascular morbimortality and determinants for its progression in the prospective advanced approach to arterial stiffness (TRIPLE-A-Stiffness) study JF - EBIOMEDICINE J2 - EBIOMEDICINE PY - 2024 SN - 2352-3964 DO - 10.1016/j.ebiom.2024.105107 UR - https://m2.mtmt.hu/api/publication/34821280 ID - 34821280 LA - English DB - MTMT ER - TY - JOUR AU - Abdisa, Kenbon Beyene AU - Némethné Szerdahelyi, Emőke AU - Molnár, Máté András AU - Friedrich, László AU - Lakner, Zoltán AU - Koris, András AU - Tóth, Attila AU - Nath, Arijit TI - Metabolic Syndrome and Biotherapeutic Activity of Dairy (Cow and Buffalo) Milk Proteins and Peptides: Fast Food-Induced Obesity Perspective—A Narrative Review JF - BIOMOLECULES J2 - BIOMOLECULES VL - 14 PY - 2024 IS - 4 SP - 478 SN - 2218-273X DO - 10.3390/biom14040478 UR - https://m2.mtmt.hu/api/publication/34820678 ID - 34820678 AB - Metabolic syndrome (MS) is defined by the outcome of interconnected metabolic factors that directly increase the prevalence of obesity and other metabolic diseases. Currently, obesity is considered one of the most relevant topics of discussion because an epidemic heave of the incidence of obesity in both developing and underdeveloped countries has been reached. According to the World Obesity Atlas 2023 report, 38% of the world population are presently either obese or overweight. One of the causes of obesity is an imbalance of energy intake and energy expenditure, where nutritional imbalance due to consumption of high-calorie fast foods play a pivotal role. The dynamic interactions among different risk factors of obesity are highly complex; however, the underpinnings of hyperglycemia and dyslipidemia for obesity incidence are recognized. Fast foods, primarily composed of soluble carbohydrates, non-nutritive artificial sweeteners, saturated fats, and complexes of macronutrients (protein-carbohydrate, starch-lipid, starch-lipid-protein) provide high metabolic calories. Several experimental studies have pointed out that dairy proteins and peptides may modulate the activities of risk factors of obesity. To justify the results precisely, peptides from dairy milk proteins were synthesized under in vitro conditions and their contributions to biomarkers of obesity were assessed. Comprehensive information about the impact of proteins and peptides from dairy milks on fast food-induced obesity is presented in this narrative review article. LA - English DB - MTMT ER - TY - JOUR AU - Farkasinszky, Gergely AU - Péliné Szabó, Judit AU - Károlyi, Péter AU - Rácz, Szilvia AU - Dénes, Noémi AU - Papp, Tamás AU - Király, József AU - Szabó, Zsuzsanna AU - Kertész, István AU - Mező, Gábor AU - Halmos, Gábor AU - Képes, Zita AU - Trencsényi, György TI - In Vivo Imaging of Acute Hindlimb Ischaemia in Rat Model: A Pre-Clinical PET Study JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 16 PY - 2024 IS - 4 SP - 542 SN - 1999-4923 DO - 10.3390/pharmaceutics16040542 UR - https://m2.mtmt.hu/api/publication/34820368 ID - 34820368 AB - Background: To better understand ischaemia-related molecular alterations, temporal changes in angiogenic Aminopeptidase N (APN/CD13) expression and glucose metabolism were assessed with PET using a rat model of peripheral arterial disease (PAD). Methods: The mechanical occlusion of the base of the left hindlimb triggered using a tourniquet was applied to establish the ischaemia/reperfusion injury model in Fischer-344 rats. 2-[18F]FDG and [68Ga]Ga-NOTA-c(NGR) PET imaging performed 1, 3, 5, 7, and 10 days post-ischaemia induction was followed by Western blotting and immunohistochemical staining for APN/CD13 in ischaemic and control muscle tissue extracts. Results: Due to a cellular adaptation to hypoxia, a gradual increase in [68Ga]Ga-NOTA-c(NGR) and 2-[18F]FDG uptake was observed from post-intervention day 1 to 7 in the ischaemic hindlimbs, which was followed by a drop on day 10. Conforming pronounced angiogenic recovery, the NGR accretion of the ischaemic extremities differed significantly from the controls 5, 7, and 10 days after ischaemia induction (p ≤ 0.05), which correlated with the Western blot and immunohistochemical results. No remarkable radioactivity was depicted between the normally perfused hindlimbs of either the ischaemic or the control groups. Conclusions: The PET-based longitudinal assessment of angiogenesis-associated APN/CD13 expression and glucose metabolism during ischaemia may continue to broaden our knowledge on the pathophysiology of PAD. LA - English DB - MTMT ER - TY - JOUR AU - Geetha, Duvuru AU - Dua, Anisha AU - Yue, Huibin AU - Springer, Jason AU - Salvarani, Carlo AU - Jayne, David AU - Merkel, Peter AU - Szűcs, Gabriella TI - Efficacy and safety of avacopan in patients with ANCA-associated vasculitis receiving rituximab in a randomised trial JF - ANNALS OF THE RHEUMATIC DISEASES J2 - ANN RHEUM DIS VL - 83 PY - 2024 IS - 2 SP - 223 EP - 232 PG - 10 SN - 0003-4967 DO - 10.1136/ard-2023-224816 UR - https://m2.mtmt.hu/api/publication/34820283 ID - 34820283 LA - English DB - MTMT ER - TY - JOUR AU - Isotta, Sturniolo AU - Csongor, Váróczy AU - Ákos, Máté Bede AU - Hegedűs, Csaba AU - Demény, Máté Ágoston AU - Virág, László TI - Quantifying antibody-dependent cellular cytotoxicity in a tumor spheroid model : application for drug discovery JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP PY - 2024 SN - 1940-087X UR - https://m2.mtmt.hu/api/publication/34820104 ID - 34820104 LA - English DB - MTMT ER -