TY - JOUR AU - Masood, Thann Oon Saleh Saeed Saleh AU - Lakatos, Szandra AU - Karcsúné Kis, Gyöngyi AU - Ignácz, Melissza AU - Domoki, Ferenc AU - Rosta, Judit TI - Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 8 PG - 18 SN - 2073-4409 DO - 10.3390/cells13080653 UR - https://m2.mtmt.hu/api/publication/34794768 ID - 34794768 AB - Subarachnoid hemorrhage (SAH) remains a major cause of cerebrovascular morbidity, eliciting severe headaches and vasospasms that have been shown to inversely correlate with vasodilator calcitonin gene-related peptide (CGRP) levels. Although dura mater trigeminal afferents are an important source of intracranial CGRP, little is known about the effects of SAH on these neurons in preclinical models. The present study evaluated changes in CGRP levels and expression in trigeminal primary afferents innervating the dura mater 72 h after experimentally induced SAH in adult rats. SAH, eliciting marked damage revealed by neurological examination, significantly reduced the density of CGRP-immunoreactive nerve fibers both in the dura mater and the trigeminal caudal nucleus in the medulla but did not affect the total dural nerve fiber density. SAH attenuated ex vivo dural CGRP release by ~40% and in the trigeminal ganglion, reduced both CGRP mRNA levels and the number of highly CGRP-immunoreactive cell bodies. In summary, we provide novel complementary evidence that SAH negatively affects the integrity of the CGRP-expressing rat trigeminal neurons. Reduced CGRP levels suggest likely impaired meningeal neurovascular functions contributing to SAH complications. Further studies are to be performed to reveal the importance of impaired CGRP synthesis and its consequences in central sensory processing. LA - English DB - MTMT ER - TY - JOUR AU - Benedicter, Nicola AU - Vogler, Birgit AU - Kuhn, Annette AU - Schramm, Jana AU - Mackenzie, Kimberly D. AU - Stratton, Jennifer AU - Dux, Mária AU - Messlinger, Karl TI - Glycerol Trinitrate Acts Downstream of Calcitonin Gene-Related Peptide in Trigeminal Nociception—Evidence from Rodent Experiments with Anti-CGRP Antibody Fremanezumab JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 7 PG - 15 SN - 2073-4409 DO - 10.3390/cells13070572 UR - https://m2.mtmt.hu/api/publication/34783723 ID - 34783723 N1 - Institute of Physiology and Pathophysiology, Friedrich-Alexander-University, Erlangen, D-91054, Germany Teva Pharmaceuticals, Redwood City, CA 94063, United States Department of Physiology, University of Szeged, Szeged, H-6720, Hungary Export Date: 6 May 2024 Correspondence Address: Messlinger, K.; Institute of Physiology and Pathophysiology, Germany; email: karl.messlinger@fau.de AB - Calcitonin gene-related peptide (CGRP) and nitric oxide (NO) have been recognized as important mediators in migraine but their mechanisms of action and interaction have not been fully elucidated. Monoclonal anti-CGRP antibodies like fremanezumab are successful preventives of frequent migraine and can be used to study CGRP actions in preclinical experiments. Fremanezumab (30 mg/kg) or an isotype control monoclonal antibody was subcutaneously injected to Wistar rats of both sexes. One to several days later, glyceroltrinitrate (GTN, 5 mg/kg) mimicking nitric oxide (NO) was intraperitoneally injected, either once or for three consecutive days. The trigeminal ganglia were removed to determine the concentration of CGRP using an enzyme-linked immunosorbent assay (ELISA). In one series of experiments, the animals were trained to reach an attractive sugar solution, the access to which could be limited by mechanical or thermal barriers. Using a semi-automated registration system, the frequency of approaches to the source, the residence time at the source, and the consumed solution were registered. The results were compared with previous data of rats not treated with GTN. The CGRP concentration in the trigeminal ganglia was generally higher in male rats and tended to be increased in animals treated once with GTN, whereas the CGRP concentration decreased after repetitive GTN treatment. No significant difference in CGRP concentration was observed between animals having received fremanezumab or the control antibody. Animals treated with GTN generally spent less time at the source and consumed less sugar solution. Without barriers, there was no significant difference between animals having received fremanezumab or the control antibody. Under mechanical barrier conditions, all behavioral parameters tended to be reduced but animals that had received fremanezumab tended to be more active, partly compensating for the depressive effect of GTN. In conclusion, GTN treatment seems to increase the production of CGRP in the trigeminal ganglion independently of the antibodies applied, but repetitive GTN administration may deplete CGRP stores. GTN treatment generally tends to suppress the animals’ activity and increase facial sensitivity, which is partly compensated by fremanezumab through reduced CGRP signaling. If CGRP and NO signaling share the same pathway in sensitizing trigeminal afferents, GTN and NO may act downstream of CGRP to increase facial sensitivity. LA - English DB - MTMT ER - TY - JOUR AU - Remzső, Gábor AU - Kovács, Viktória AU - Tóth-Szűki, Valéria AU - Domoki, Ferenc TI - The effects of CO2 levels and body temperature on brain interstitial pH alterations during the induction of hypoxic-ischemic encephalopathy in newborn pigs JF - HELIYON J2 - HELIYON VL - 10 PY - 2024 IS - 7 PG - 11 SN - 2405-8440 DO - 10.1016/j.heliyon.2024.e28607 UR - https://m2.mtmt.hu/api/publication/34780663 ID - 34780663 N1 - Export Date: 10 April 2024 Correspondence Address: Remzső, G.; Department of Physiology, Hungary; email: remzso.gabor@med.u-szeged.hu AB - Brain interstitial pH (pHbrain) alterations play a crucial role in the development of hypoxic-ischemic (HI) encephalopathy (HIE) caused by asphyxia in neonates. The newborn pig is one of the most suitable large animal models for studying HIE, however, compared to rats, experimental data on pHbrain alterations during HIE induction are limited. The major objective of the present study was thus to compare pHbrain changes during HIE development induced by experimental normocapnic hypoxia (H) or asphyxia (A), elicited with ventilation of a gas mixture containing 6%O2 or 6%O2/20%CO2, respectively for 20 min, under either normothermia (NT) or hypothermia (HT) (38.5 ± 0.5 °C or 33.5 ± 0.5 °C core temperature, respectively) in anesthetized piglets yielding four groups: H-NT, A-NT, H-HT, and A-HT. pHbrain changes during HI stress and the 60 min reoxygenation period were measured using a pH-selective microelectrode inserted into the parietal cortex through an open cranial window. In all groups, the pHbrain response to HI stress was acidosis, at the nadir pHbrain values dropped from the baseline of 7.27 ± 0.02 to H-NT:5.93 ± 0.30, A-NT:5.90 ± 0.52, H-HT:6.81 ± 0.27, and A-HT:6.27 ± 0.24 indicating that (1) H and A elicited similar, severe brain acidosis under NT greatly exceeding pH changes in arterial blood (pHa dropped to 7.24 ± 0.07 and 6.78 ± 0.03 from 7.52 ± 0.06 and 7.50 ± 0.05, respectively), and (2) HT ameliorated more the brain acidosis induced by H than by A. In all four groups, pHbrain was restored to baseline values without an alkalotic overshoot during the observed reoxygenation, Our findings suggest that under NT either H or A – both commonly employed HI stresses to elicit HIE in piglet models – would result in a similar acidotic pHbrain response without an alkalotic component either during the HI stress or the early reoxygenation period. © 2024 The Authors LA - English DB - MTMT ER - TY - JOUR AU - Tuboly, Gábor AU - Horváth, Gyöngyi AU - Nagy, Kamilla AU - Nagy, Edit TI - Personalized analysis of pain–weather associations: a pilot study JF - IDEGGYOGYASZATI SZEMLE / CLINICAL NEUROSCIENCE J2 - IDEGGYOGY SZEMLE VL - 77 PY - 2024 IS - 3-4 SP - 77 EP - 87 PG - 11 SN - 0019-1442 DO - 10.18071/isz.77.0077 UR - https://m2.mtmt.hu/api/publication/34779925 ID - 34779925 AB - Background and purpose – It is a wellknown belief that weather can influence human health, including pain sensation. However, the current data are controversial, which might be due to the wide range of interindividual differences. The present study aimed to characterize the individual pain– weather associations during chronic pain by utilizing several data analytical methods. Methods – The study included 3-3 patients with (P1, P3, and P4) or without (P2, P5, P6) diabetes mellitus and signs of trigeminal neuralgia or low back pain. Subjective pain scores (0–10) and 12 weather parameters (terrestrial, geomagnetic, and solar) were recorded for one month repeated three times daily. Nonparametric Spearman’s correlation (Sp), multiple regression (Mx), and principal component (PCA) analyses were performed to evaluate associations between pain and meteorological factors obtained at the day of recorded pain value, 2 days before and 2 days after the recorded pain, and the changes in these parameters (5 × 12 parameters). Complex scores were calculated based on the results of these analyses. Results – While the temperature had the highest effects on the pain levels in most of the participants, huge interindividual dif­ferences in the degree and the direction of the associations between pain and weather parameters could be obtained. The analytic methods also revealed subjectspecific results, and the synthesis of different statistical methods as total scores provided a personalized map for each patient, which showed disparate patterns across the study participants. Thus, Participants 2 and 5 had higher scores for Mx compared to Sp; furthermore, certain factors showed opposite direction in their associations with the pain level depending on the type of analysis (Sp vs Mx). In contrast, P3 had a lower score for Mx compared to Sp, which might suggest a low level of weather sensitivity on the association between the different weather parameters in this subject. Furthermore, participants P4 and P6 had a very high level of weather sensitivity, while P1 had an opposite pattern. Regarding the time point-related effects on the pain level, most patients were sensitive to parameters obtained at the same day or two days before, except the P1 subject, who had the highest sensitivity to weather parameters detected two days after. Conclusion – The present study highlights the importance of integrating different data analysis approaches to elucidate the individual connections between pain and most of the weather parameters. In conclusion, complex personalized profiling should be considered for the characterization of pain–weather associations by applying different data analytical approaches, which may provide feedback to physicians and patients. LA - English DB - MTMT ER - TY - CONF AU - Plesz, Szonja Bianka AU - Adlan, Leatitia Gabriella AU - Büki, Alexandra AU - Horváth, Gyöngyi AU - Ligeti, Balázs AU - Zádori, Zoltán Sándor AU - Kékesi, Gabriella TI - Exploring the gut-brain connection: How does microbiome composition relate to cognitive behavior in Wisket model rats? T2 - 7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet PY - 2024 SP - 86 UR - https://m2.mtmt.hu/api/publication/34577105 ID - 34577105 LA - English DB - MTMT ER - TY - CONF AU - Adlan, Leatitia Gabriella AU - Plesz, Szonja Bianka AU - Büki, Alexandra AU - Horváth, Gyöngyi AU - Ligeti, Balázs AU - Zádori, Zoltán Sándor AU - Kékesi, Gabriella TI - The Firmicutes/Bacteroidetes ratio in the triple-hit Wisket model rats of schizophrenia T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - 74 UR - https://m2.mtmt.hu/api/publication/34577022 ID - 34577022 LA - English DB - MTMT ER - TY - CONF AU - Plesz, Szonja Bianka AU - Adlan, Leatitia Gabriella AU - Büki, Alexandra AU - Ligeti, Balázs AU - Zádori, Zoltán Sándor AU - Kékesi, Gabriella TI - Is gut dysbiosis associated with the motivational deficit observed in Wisket animals? T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - 73 UR - https://m2.mtmt.hu/api/publication/34576951 ID - 34576951 LA - English DB - MTMT ER - TY - CONF AU - Eördegh, Gabriella AU - Braunitzer, Gábor AU - Tót, Kálmán AU - Kiss, Ádám AU - Kóbor, Jenő Dezső AU - Nagy, Attila TI - Altered multisensory integration in pediatric migraine without aura T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - 198 EP - 198 PG - 1 UR - https://m2.mtmt.hu/api/publication/34551935 ID - 34551935 AB - Alterations of visual processing in migraine are well known. It seems to be that multisensory information processing is altered in migraine, too, but this aspects of migraine are strongly underex- plored, especially regarding pediatric migraine. In the present study performances in visual and audiovisual associative equivalence learning of the pediatric patients with migraine without aura (aged 7-17.5 years) were compared to those of age- and sex-matched controls. The application of audiovisual stimuli significantly facilitated associative pair learning in healthy children and adolescents, but not in pediatric migraine patients. Although the performances were not significant worse in pediatric migraineurs compared those of the controls but the multisensory gain is significantly reduced in these patients. The results of this study suggest that multisensory integration is altered not only in adult mi- graineurs but this dysfunction can be already observed in pediatric migraine patients, too. LA - English DB - MTMT ER - TY - CONF AU - Tót, Kálmán AU - Eördegh, Gabriella AU - Bátor, Brassó AU - Harcsa-Pintér, Noémi AU - Kiss, Ádám AU - Bodosi, Balázs AU - Kelemen, András AU - Nagy, Attila TI - The effect of the applied visual stimuli with different level of complexity on audiovisual equivalence learning T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - 196 EP - 196 PG - 1 UR - https://m2.mtmt.hu/api/publication/34551903 ID - 34551903 N1 - Supported by SZTE SZAOK- KKA-SZGYA Grant No.:2023/5S479 AB - The Rutgers Acquired Equivalence Test (RAET) is a learning task, where the subjects learn visual stimuli pairs, and after the learning, they have to retrieve and generalize the previously learned as- sociations. We developed three audiovisual equivalence learning tests with the same structure, in which the antecedents were the same four different distinguishable sounds, but the consequents differed in complexity and semantic meanings. In the SoundFace test the consequents were four drawn faces, in the SoundFish four different colored fish (all same in size and shape), and in the SoundPolygon test blank geometric shapes. In the present study we compared the psychophysi- cal performances of 52 healthy volunteers between the three audiovisual tests. We asked whether there is any difference between the performances when visual stimuli differ in complexity and se- mantic meanings. In all parts (learning, retrieval and generalization) of the tests, the performance was significantly better in the SoundFace test compared to the other two, but there was no signifi- cant difference between the SoundFish and SoundPolygon. Our results suggest that the color infor- mation alone could not significantly enhance the effectiveness of the associative learning. However, the verbalizability of individual features of the visual stimuli seem to determine primarily the perfor- mances in associative equivalence learning. LA - English DB - MTMT ER - TY - CONF AU - Kiss, Ádám AU - Kelemen, András AU - Nagy, Attila TI - Blink rate extraction from EEG recording T2 - 7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet PY - 2024 SP - 57 EP - 57 PG - 1 UR - https://m2.mtmt.hu/api/publication/34551651 ID - 34551651 N1 - The study was supported by the SZTE SZAOK-KKA-SZGYA (No. 2023/5S479) grant from University of Szeged. AB - EEG recordings include many components, such as EMG or blink components. In classical processing these are excluded, however, they may contain additional incomes. A possible side-channel information is the blink rate. A possible extraction of it is presented in this paper. LA - English DB - MTMT ER -