@article{MTMT:34836454,
	title = {Short-term hyperoxia-induced functional and morphological changes in rat hippocampus},
	url = {https://m2.mtmt.hu/api/publication/34836454},
	author = {Hencz, Alexandra Júlia and Magony, Andor Dániel and Thomas, Chloe and Kovacs, Krisztina and Szilágyi, Tamás Gábor and Pál, József and Sík, Attila},
	doi = {10.3389/fncel.2024.1376577},
	journal-iso = {FRONT CELL NEUROSCI},
	journal = {FRONTIERS IN CELLULAR NEUROSCIENCE},
	volume = {18},
	unique-id = {34836454},
	issn = {1662-5102},
	abstract = {Excess oxygen (O2) levels may have a stimulating effect, but in the long term, and at high concentrations of O2, it is harmful to the nervous system. The hippocampus is very sensitive to pathophysiological changes and altered O2 concentrations can interfere with hippocampus-dependent learning and memory functions. In this study, we investigated the hyperoxia-induced changes in the rat hippocampus to evaluate the short-term effect of mild and severe hyperoxia. Wistar male rats were randomly divided into control (21% O2), mild hyperoxia (30% O2), and severe hyperoxia groups (100% O2). The O2 exposure lasted for 60 min. Multi-channel silicon probes were used to study network oscillations and firing properties of hippocampal putative inhibitory and excitatory neurons. Neural damage was assessed using the Gallyas silver impregnation method. Mild hyperoxia (30% O2) led to the formation of moderate numbers of silver-impregnated "dark" neurons in the hippocampus. On the other hand, exposure to 100% O2 was associated with a significant increase in the number of "dark" neurons located mostly in the hilus. The peak frequency of the delta oscillation decreased significantly in both mild and severe hyperoxia in urethane anesthetized rats. Compared to normoxia, the firing activity of pyramidal neurons under hyperoxia increased while it was more heterogeneous in putative interneurons in the cornu ammonis area 1 (CA1) and area 3 (CA3). These results indicate that short-term hyperoxia can change the firing properties of hippocampal neurons and network oscillations and damage neurons. Therefore, the use of elevated O2 concentration inhalation in hospitals (i.e., COVID treatment and surgery) and in various non-medical scenarios (i.e., airplane emergency O2 masks, fire-fighters, and high altitude trekkers) must be used with extreme caution.},
	keywords = {hippocampus; Electrophysiology; HYPEROXIA; network oscillation; Dark neuron},
	year = {2024},
	eissn = {1662-5102}
}

@article{MTMT:34832995,
	title = {Changes in the Carotenoids of Zamia dressleri Leaves during Development},
	url = {https://m2.mtmt.hu/api/publication/34832995},
	author = {Murillo, Enrique and Nagy, Veronika and Menchaca, Dania and Deli, József and Agócs, Attila},
	doi = {10.3390/plants13091251},
	journal-iso = {PLANTS-BASEL},
	journal = {PLANTS-BASEL},
	volume = {13},
	unique-id = {34832995},
	abstract = {It has been observed that the leaves of some Zamia species undergo a kind of “reverse ripening”; that is, they change from their original brown color to green during development. We assumed that this strange color change was due to the change in carotenoid composition, so we followed the changes for several weeks. The detailed carotenoid composition and content at different stages of development of the leaves was determined with HPLC-DAD focusing on the changes in red and yellow carotenoids. The total and relative amounts of red and yellow carotenoids were determined simultaneously from one measurement from a saponified and/or unsaponified extract. At the beginning of development, the concentration of red carotenoids was higher than that of the yellow ones; it decreased drastically until 22 days and continued to decrease slowly until they completely disappeared. The concentration of yellow carotenoids decreased at the beginning as well, but after 22 days it started to increase. The amount of red carotenoids started to decrease when the leaflet stopped growing. Lutein is the main component in old leaflets, which is not a red carotenoid precursor. Red carotenoids can always be found in their esterified form in the leaves. These findings support the hypothesis that red and yellow carotenoid accumulation are independent and probably have different functions in the leaflet. The strange color change was explained based on the compartmentalization of red and yellow carotenoids and on the changing activity of the enzyme capsanthin–capsorubin synthase responsible for the synthesis of red carotenoids capsorubin and capsanthin.},
	year = {2024},
	eissn = {2223-7747},
	orcid-numbers = {Nagy, Veronika/0000-0002-9019-7980; Deli, József/0000-0002-0625-6117}
}

@CONFERENCE{MTMT:34813110,
	title = {Fatty acid changes in gestational diabetes patients and healthy pregnant women},
	url = {https://m2.mtmt.hu/api/publication/34813110},
	author = {Frank, Eszter Anita and Pergel, Mónika and Szabó, Éva and Ungár Tamás Lászlóné Polyák, Éva},
	booktitle = {Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference},
	unique-id = {34813110},
	year = {2024},
	pages = {19-19},
	orcid-numbers = {Ungár Tamás Lászlóné Polyák, Éva/0000-0002-3648-1614}
}

@CONFERENCE{MTMT:34813000,
	title = {Comparison of Quality Parameters and Fatty Acid Content of Fish Oil Supplements Across Countries},
	url = {https://m2.mtmt.hu/api/publication/34813000},
	author = {Kaçar, Ömer Furkan and Szentes, T and Verzár, Zsófia and Poór, Viktória and Marosvölgyi, Tamás and Szabó, Éva},
	booktitle = {Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference},
	unique-id = {34813000},
	year = {2024},
	pages = {17-17},
	orcid-numbers = {Verzár, Zsófia/0000-0001-5323-0748; Marosvölgyi, Tamás/0000-0002-4244-5513}
}

@article{MTMT:34790193,
	title = {Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model},
	url = {https://m2.mtmt.hu/api/publication/34790193},
	author = {Faragó, Anna and Zvara, Ágnes and Tiszlavicz, László and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Hegedűs, Zoltán and Szabó, Enikő and Surguta, Sára Eszter and Tóvári, József and Puskás, László and Szebeni, Gábor},
	doi = {10.3390/ijms25074022},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34790193},
	issn = {1661-6596},
	abstract = {A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies.},
	keywords = {colorectal carcinoma; lectin binding sugar code; proteomic analysis of murine CRC},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Tiszlavicz, László/0000-0003-1134-6587; Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34775247,
	title = {Exploring the Chemical Profile, In Vitro Antioxidant and Anti-Inflammatory Activities of Santolina rosmarinifolia Extracts},
	url = {https://m2.mtmt.hu/api/publication/34775247},
	author = {Schmidt, János and Juhász, Kata and Bóna, Ágnes},
	doi = {10.3390/molecules29071515},
	journal-iso = {MOLECULES},
	journal = {MOLECULES},
	volume = {29},
	unique-id = {34775247},
	issn = {1420-3049},
	abstract = {In this study, the phytochemical composition, in vitro antioxidant, and anti-inflammatory effects of the aqueous and 60% ethanolic (EtOH) extracts of Santolina rosmarinifolia leaf, flower, and root were examined. The antioxidant activity of S. rosmarinifolia extracts was determined by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The total phenolic content (TPC) of the extracts was measured by the Folin–Ciocalteu assay. The anti-inflammatory effect of the extracts was monitored by the Griess assay. The chemical composition of S. rosmarinifolia extracts was analysed using the LC-MS technique. According to our findings, 60% EtOH leaf extracts showed the highest Trolox equivalent antioxidant capacity (TEAC) values in both ABTS (8.39 ± 0.43 µM) and DPPH (6.71 ± 0.03 µM) antioxidant activity assays. The TPC values of the samples were in good correspondence with the antioxidant activity measurements and showed the highest gallic acid equivalent value (130.17 ± 0.01 µg/mL) in 60% EtOH leaf extracts. In addition, the 60% EtOH extracts of the leaves were revealed to possess the highest anti-inflammatory effect. The LC-MS analysis of S. rosmarinifolia extracts proved the presence of ascorbic acid, catalpol, chrysin, epigallocatechin, geraniol, isoquercitrin, and theanine, among others, for the first time. However, additional studies are needed to investigate the direct relationship between the chemical composition and physiological effects of the herb. The 60% EtOH extracts of S. rosmarinifolia leaves are potential new sources of natural antioxidants and anti-inflammatory molecules in the production of novel nutraceutical products.},
	year = {2024},
	eissn = {1420-3049}
}

@article{MTMT:34753763,
	title = {An Alternative Application of Magnetic-Activated Cell Sorting: CD45 and CD235a Based Purification of Semen and Testicular Tissue Samples},
	url = {https://m2.mtmt.hu/api/publication/34753763},
	author = {Czétány, Péter and Balló, András and Márk, László and Török, Attila and Szántó, Árpád László and Máté, Gábor},
	doi = {10.3390/ijms25073627},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34753763},
	issn = {1661-6596},
	abstract = {Magnetic activated cell sorting (MACS) is a well-known sperm selection technique, which is able to remove apoptotic spermatozoa from semen samples using the classic annexinV based method. Leukocytes and erythrocytes in semen samples or in testicular tissue processed for in vitro fertilization (IVF) could exert detrimental effects on sperm. In the current study, we rethought the aforementioned technique and used magnetic microbeads conjugated with anti-CD45/CD235a antibodies to eliminate contaminating leukocytes and erythrocytes from leukocytospermic semen samples and testicular tissue samples gained via testicular sperm extraction (TESE). With this technique, a 15.7- and a 30.8-fold reduction could be achieved in the ratio of leukocytes in semen and in the number of erythrocytes in TESE samples, respectively. Our results show that MACS is a method worth to reconsider, with more potential alternative applications. Investigations to find molecules labeling high-quality sperm population and the development of positive selection procedures based on these might be a direction of future research.},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Márk, László/0000-0002-9301-8159}
}

@article{MTMT:34745300,
	title = {Antibiotics and probiotics-induced effects on the total fatty acid composition of feces in a rat model},
	url = {https://m2.mtmt.hu/api/publication/34745300},
	author = {Marosvölgyi, Tamás and Mintál, Kitti and Borbásné Farkas, Kornélia and Sipos, Zoltán and Makszin, Lilla and Szabó, Éva and Tóth, Attila and Kocsis, Béla and Kovács, Krisztina and Hormay, Edina and Lénárd, László and Karádi, Zoltán György and Bufa, Anita},
	doi = {10.1038/s41598-024-57046-6},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {14},
	unique-id = {34745300},
	issn = {2045-2322},
	abstract = {Fatty acids (FAs) play important roles as membrane components and signal transduction molecules. Changes in short chain FA (SCFA) composition are associated with gut microbiota modifications. However, the effect of bacteria-driven changes on the detailed FA spectrum has not been explored yet. We investigated the effect of antibiotics (ABx) and/or probiotics, in four treatment groups on rat stool FA composition. Principal component analysis indicated that the chromatogram profiles of the treatment groups differ, which was also observed at different time points. Linear mixed effects models showed that in the parameters compared (sampling times, treatments. and their interactions), both the weight percentage and the concentration of FAs were affected by ABx and probiotic administration. This study found that the gut microbiome defines trans and branched saturated FAs, most saturated FAs, and unsaturated FAs with less carbon atoms. These results are among the first ones to demonstrate the restoring effects of a probiotic mixture on a substantial part of the altered total FA spectrum, and also revealed a previously unknown relationship between gut bacteria and a larger group of FAs. These findings suggest that intestinal bacteria produce not only SCFAs but also other FAs that may affect the host’s physiological processes.},
	year = {2024},
	eissn = {2045-2322},
	orcid-numbers = {Marosvölgyi, Tamás/0000-0002-4244-5513; Mintál, Kitti/0009-0003-4739-2902; Borbásné Farkas, Kornélia/0000-0002-5349-6527; Sipos, Zoltán/0000-0001-7845-8116; Makszin, Lilla/0000-0002-9764-4763; Hormay, Edina/0009-0004-0598-5061}
}

@article{MTMT:34724787,
	title = {Trans isomeric fatty acids in human milk and their role in infant health and development},
	url = {https://m2.mtmt.hu/api/publication/34724787},
	author = {Hatem, Okba and Kaçar, Ömer Furkan and Kaçar, Hüsna Kaya and Szentpéteri, József László (Joe Petersburger) and Marosvölgyi, Tamás and Szabó, Éva},
	doi = {10.3389/fnut.2024.1379772},
	journal-iso = {FRONT NUTR},
	journal = {FRONTIERS IN NUTRITION},
	volume = {11},
	unique-id = {34724787},
	issn = {2296-861X},
	abstract = {It is well known that long chain polyunsaturated fatty acids (LCPUFAs) play an important role in neurodevelopment in the perinatal life. The most important source of these fatty acids is the diet, however, they can also be formed in the human body from their shorter chain precursors, the essential fatty acids. Since the WHO recommends exclusive breastfeeding for the first six months after birth, the exclusive source of these fatty acids for breastfed infants is human milk, which can be influenced by the mother’s diet. Unsaturated fatty acids can have either cis or trans configuration double bond in their chain with distinct physiological effects. Cis isomeric unsaturated fatty acids have several beneficial effects, while trans isomers are mostly detrimental, because of their similar structure to saturated fatty acids. Trans fatty acids (TFAs) can be further subdivided into industrial (iTFA) and ruminant-derived trans fatty acids (rTFA). However, the physiological effects of these two TFA subgroups may differ. In adults, dietary intake of iTFA has been linked to atherosclerosis, insulin resistance, obesity, chronic inflammation, and increased development of certain cancers, among other diseases. However, iTFAs can have a negative impact on health not only in adulthood but in childhood too. Results from previous studies have shown that iTFAs have a significant negative effect on LCPUFA levels in the blood of newborns and infants. In addition, iTFAs can affect the growth and development of infants, and animal studies suggest that they might even have lasting negative effects later in life. Since the only source of TFAs in the human body is the diet, the TFA content of breast milk may determine the TFA supply of breastfed infants and thus affect the levels of LCPUFAs important for neurodevelopment and the health of infants. In this review, we aim to provide an overview of the TFA content in human milk available in the literature and their potential effects on infant health and development.},
	year = {2024},
	eissn = {2296-861X},
	orcid-numbers = {Marosvölgyi, Tamás/0000-0002-4244-5513}
}

@article{MTMT:34723100,
	title = {Comprehensive within-population characterization of the Transylvanian Seklers based on high-resolution autosomal marker data},
	url = {https://m2.mtmt.hu/api/publication/34723100},
	author = {Bánfai, Zsolt and Adam, Valerian and Sümegi, Katalin and Szabo, Andras and Büki, Gergely and Magyari, Lili and Hadzsiev, Kinga and Kasler, Miklos and Melegh, Bela},
	journal-iso = {EUR J HUM GENET},
	journal = {EUROPEAN JOURNAL OF HUMAN GENETICS},
	volume = {32},
	unique-id = {34723100},
	issn = {1018-4813},
	keywords = {Biochemistry & Molecular Biology},
	year = {2024},
	eissn = {1476-5438},
	pages = {313-313}
}