TY - JOUR AU - Pap, Domonkos AU - Pajtók, Csenge AU - Veres-Székely, Apor AU - Szebeni, Beáta AU - Szász, Csenge AU - Bokrossy, Péter AU - Zrufkó, Réka AU - Vannay, Ádám AU - Tulassay, Tivadar AU - Szabó, Attila TI - High Salt Promotes Inflammatory and Fibrotic Response in Peritoneal Cells JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 18 PG - 19 SN - 1661-6596 DO - 10.3390/ijms241813765 UR - https://m2.mtmt.hu/api/publication/34135659 ID - 34135659 N1 - Pediatric Center, MTA Center of Excellence, Semmelweis University, Budapest, 1085, Hungary HUN-REN–SU Pediatrics and Nephrology Research Group, Budapest, 1052, Hungary Export Date: 9 January 2024 Correspondence Address: Pap, D.; Pediatric Center, Hungary; email: pap.domonkos@med.semmelweis-univ.hu AB - Recent studies draw attention to how excessive salt (NaCl) intake induces fibrotic alterations in the peritoneum through sodium accumulation and osmotic events. The aim of our study was to better understand the underlying mechanisms. The effects of additional NaCl were investigated on human primary mesothelial cells (HPMC), human primary peritoneal fibroblasts (HPF), endothelial cells (HUVEC), immune cells (PBMC), as well as ex vivo on peritoneal tissue samples. Our results showed that a high-salt environment and the consequently increased osmolarity increase the production of inflammatory cytokines, profibrotic growth factors, and components of the renin–angiotensin–aldosterone system, including IL1B, IL6, MCP1, TGFB1, PDGFB, CTGF, Renin and Ace both in vitro and ex vivo. We also demonstrated that high salt induces mesenchymal transition by decreasing the expression of epithelial marker CDH1 and increasing the expression of mesenchymal marker ACTA2 and SNAIL1 in HPMCs, HUVECs and peritoneal samples. Furthermore, high salt increased extracellular matrix production in HPFs. We demonstrated that excess Na+ and the consequently increased osmolarity induce a comprehensive profibrotic response in the peritoneal cells, thereby facilitating the development of peritoneal fibrosis. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Kata AU - Giesinger, Regan E. AU - Lakatos, Andrea AU - Szabó, Attila AU - Szabó, Miklós AU - Jermendy, Ágnes AU - McNamara, Patrick J. TI - Comparative evaluation of approach to cardiovascular care in neonatal encephalopathy undergoing therapeutic hypothermia JF - JOURNAL OF PERINATOLOGY J2 - J PERINATOL VL - 42 PY - 2022 IS - 12 SP - 1637 EP - 1643 PG - 7 SN - 0743-8346 DO - 10.1038/s41372-022-01459-6 UR - https://m2.mtmt.hu/api/publication/33028317 ID - 33028317 LA - English DB - MTMT ER - TY - JOUR AU - Becsei, Dóra AU - Kiss, Erika AU - Szatmári, Ildikó AU - Arató, András AU - Reusz, György AU - Szabó, Attila AU - Bókay, János AU - Zsidegh, Petra TI - A retrospective analysis of metabolic control in children with PKU in the COVID-19 era JF - MOLECULAR GENETICS AND METABOLISM REPORTS J2 - MOL GENET METAB REP VL - 32 PY - 2022 PG - 7 SN - 2214-4269 DO - 10.1016/j.ymgmr.2022.100897 UR - https://m2.mtmt.hu/api/publication/33023411 ID - 33023411 LA - English DB - MTMT ER - TY - JOUR AU - Sepsi, Zita AU - Bokrossy, Péter AU - Szász, Csenge AU - Pap, Domonkos AU - Szebeni, Beáta AU - Szabó, Attila AU - Vannay, Ádám AU - Veres-Székely, Apor TI - A fibroblastok funkcionális vizsgálata – II. rész: Sejtmigrációs mérések JF - GYERMEKGYÓGYÁSZAT J2 - GYERMEKGYÓGYÁSZAT VL - 73 PY - 2022 IS - 4 SP - 252 EP - 254 PG - 3 SN - 0017-5900 UR - https://m2.mtmt.hu/api/publication/33002115 ID - 33002115 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Méder, Ünőke AU - Csekő, Anna Judit AU - Szakacs, Laszlo AU - Balogh, Dóra Csenge AU - Szakmár, Enikő AU - Andorka, Csilla AU - Kovács, Kata AU - Dobi, Marianna AU - Brandt, Ferenc AU - Szabó, Miklós AU - Szabó, Attila AU - Jermendy, Ágnes TI - Longitudinal Analysis of Amplitude-Integrated Electroencephalography for Outcome Prediction in Hypoxic-Ischemic Encephalopathy JF - JOURNAL OF PEDIATRICS J2 - J PEDIATR VL - 246 PY - 2022 SP - 19 EP - 25.e5 PG - 12 SN - 0022-3476 DO - 10.1016/j.jpeds.2022.04.013 UR - https://m2.mtmt.hu/api/publication/32962031 ID - 32962031 AB - Objective To investigate the prognostic accuracy of longitudinal analysis of amplitude-integrated electroencephalography (aEEG) background activity to predict long-term neurodevelopmental outcome in neonates with hypoxic-ischemic encephalopathy (HIE) receiving therapeutic hypothermia.Study design This single-center observational study included 149 neonates for derivation and 55 neonates for validation with moderate-severe HIE and of gestational age 35 weeks at a tertiary neonatal intensive care unit. Single-channel aEEG background pattern, sleep-wake cycling, and seizure activity were monitored over 84 hours during therapeutic hypothermia and rewarming, then scored for each 6-hour interval. Neurodevelopmental outcome was assessed using the Bayley Scales of Infant Development, Second Edition. Favorable outcome was defined as having both a Mental Development Index (MDI) score and Psychomotor Development Index (PDI) score >= 70, and adverse outcome was defined as either an MDI or a PDI <70 or death. Regression modeling for longitudinal analysis of repeatedly measured data was applied, and area under the receiver operating characteristic curve (AUC) was calculated.Resuits Longitudinal aEEG background analysis combined with sleep-wake cycling score had excellent predictive value (AUC, 0.90; 95% CI, 0.85-0.95), better than single aEEG scores at any individual time point. The model performed well in the independent validation cohort (AUC, 0.87; 95% CI, 0.62-1.00). The reclassification rate of this model compared with the conventional analysis of aEEG background at 48 hours was 18% (24 patients); 14% (18 patients) were reclassified correctly. Our results were used to develop a user-friendly online outcome prediction tool.Conclusions Longitudinal analysis of aEEG background activity and sleep-wake cycling is a valuable and accurate prognostic tool. LA - English DB - MTMT ER - TY - GEN AU - Lakat, Tamás AU - Hosszú, Ádám AU - Tóth, Ákos Roland AU - Molnár, Ágnes AU - Varga, Zoltán Kristóf AU - Kelemen, Hanga AU - Szabó, Attila AU - Szabó, Miklós AU - Fekete, Andrea TI - Perinatalis aszfixiát követő többszervi károsodás patkány modellben PY - 2022 PG - 1 UR - https://m2.mtmt.hu/api/publication/32925862 ID - 32925862 N1 - 2017-1.3.1-VKE-2017-00006, OTKA (FK124491, PD131637, K135398), 2020-4.1.1-TKP2020-(6183069269, 6183169273), ÚNKP-20-4-II-SE-13, KDP-2020/1019145, ÚNKP-21-3-II-SE-58 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Pap, Domonkos AU - Pajtók, Csenge AU - Veres-Székely, Apor AU - Szebeni, Beáta AU - Lévai, Eszter AU - Reusz, György AU - Szabó, Attila AU - Vannay, Ádám TI - Új terápiás lehetőségek az akut vesekárosodás kezelésében JF - HYPERTONIA ÉS NEPHROLOGIA J2 - HYPERTONIA NEPHROLOGIA VL - 26 PY - 2022 IS - Suppl .2 SP - 41 EP - 42 PG - 2 SN - 1418-477X UR - https://m2.mtmt.hu/api/publication/32925822 ID - 32925822 N1 - 20382-3/2018 FEKUTSTRAT, STIA-KFI-2020 (61830-96319), ÚNKP-20-3-I-SE-49, FIKP(61830-69271), TKP2020-NKA-09, TKP2020-NKA-13, K125470, NKFI-124549 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Veres-Székely, Apor AU - Lévai, Eszter AU - Szebeni, Beáta AU - Szász, Csenge AU - Pap, Domonkos AU - Pajtók, Csenge AU - Reusz, György AU - Szabó, Attila AU - Vannay, Ádám TI - A PARK7 szerepe a peritonealis dialízis-asszociált fibrózisban JF - HYPERTONIA ÉS NEPHROLOGIA J2 - HYPERTONIA NEPHROLOGIA VL - 26 PY - 2022 IS - Suppl. 2 SP - 46 EP - 47 PG - 2 SN - 1418-477X UR - https://m2.mtmt.hu/api/publication/32925798 ID - 32925798 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szebeni, Beáta AU - Varga, Zoltán AU - Takács, István Márton AU - Pállinger, Éva AU - Pap, Domonkos AU - Veres-Székely, Apor AU - Szabó, Attila AU - Reusz, György AU - Vannay, Ádám TI - Extracelluláris vezikulák (EV) szerepe a peritonealis fibrózis kialakulásában JF - HYPERTONIA ÉS NEPHROLOGIA J2 - HYPERTONIA NEPHROLOGIA VL - 26 PY - 2022 IS - Suppl.2 SP - 45 EP - 45 PG - 1 SN - 1418-477X UR - https://m2.mtmt.hu/api/publication/32925740 ID - 32925740 N1 - 20382-3/FEKUTSTRAT, STIA-KFI-2000 (61830-96319), ÚNKP-20-3-I-SE-49, FIKP (61830-69271), TKP2020-NKA-09, TKP2020-NKA-14, K125470, K124549 LA - Hungarian DB - MTMT ER - TY - CONF AU - Tran, Ngoc Minh AU - Besztercei, Balázs AU - Torok, Gyorgy AU - Medveczki, Tímea AU - Kovács, Illés AU - Szabó, Attila AU - Fekete, Andrea AU - Hodrea, Judit TI - The protective role of fluvoxamine in fibrotic disorders of the eye T2 - 15th EGS Congress PY - 2022 SP - 44 EP - 44 PG - 1 UR - https://m2.mtmt.hu/api/publication/32924364 ID - 32924364 N1 - Grants: OTKA- K135398, LP2021-3/2021, 2020-4.1.1.-TKP2020-6183069269 (FIKP), 2020-4.1.1.-TKP2020-6183169273. LA - English DB - MTMT ER -