TY - JOUR AU - Jankó, Laura AU - Tóth, Emese AU - Laczik, Miklós AU - Rauch, Boglárka AU - Janka, Eszter AU - Bálint, Bálint László AU - Bay, Péter TI - PARP2 poly(ADP-ribosyl)ates nuclear factor erythroid 2-related factor 2 (NRF2) affecting NRF2 subcellular localization JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-023-35076-w UR - https://m2.mtmt.hu/api/publication/33870536 ID - 33870536 AB - PARP2 is a member of the PARP enzyme family. Although, PARP2 plays role in DNA repair, it has regulatory roles in mitochondrial and lipid metabolism, it has pivotal role in bringing about the adverse effects of pharmacological PARP inhibitors. Previously, we showed that the ablation of PARP2 induces oxidative stress and, consequently, mitochondrial fragmentation. In attempt to identify the source of the reactive species we assessed the possible role of a central regulator of cellular antioxidant defense, nuclear factor erythroid 2-related factor 2 (NRF2). The silencing of PARP2 did not alter either the mRNA or the protein expression of NRF2, but changed its subcellular localization, decreasing the proportion of nuclear, active fraction of NRF2. Pharmacological inhibition of PARP2 partially restored the normal localization pattern of NRF2 and in line with that, we showed that NRF2 is PARylated that is absent in the cells in which PARP2 was silenced. Apparently, the PARylation of NRF2 by PARP2 has pivotal role in regulating the subcellular (nuclear) localization of NRF2. The silencing of PARP2 rearranged the expression of genes encoding proteins with antioxidant function, among these a subset of NRF2-dependent genes. LA - English DB - MTMT ER - TY - JOUR AU - Jankó, Laura AU - Kovács, Tünde AU - Laczik, Miklós AU - Sári, Zsanett Mercédesz AU - Ujlaki, Gyula AU - Kis, Nikoletta Gréta AU - Horváth, Ibolya AU - Antal, Miklós AU - Vigh, László AU - Bálint, Bálint László AU - Uray (Davis), Karen L. AU - Bay, Péter TI - Silencing of Poly(ADP-Ribose) Polymerase-2 Induces Mitochondrial Reactive Species Production and Mitochondrial Fragmentation JF - CELLS J2 - CELLS-BASEL VL - 10 PY - 2021 IS - 6 PG - 23 SN - 2073-4409 DO - 10.3390/cells10061387 UR - https://m2.mtmt.hu/api/publication/32058440 ID - 32058440 N1 - Funding Agency and Grant Number: NKFIHNational Research, Development & Innovation Office (NRDIO) - Hungary [K123975, GINOP-2.3.2-15-201600006, EFOP-3.6.2-16-2017-00006, K 129166]; Momentum fellowship of the Hungarian Academy of Sciences; University of Debrecen; Higher Education Institutional Excellence Program of the Ministry of Innovation and Technology in Hungary [NKFIH-1150-6/2019]; MOLMEDEX FUN-OMICS [GINOP-2.3.3-15-2016-00007]; Debrecen Venture Catapult Program [EFOP-3.6.1-16-2016-00022]; [UNKP-20-4-II-DE-68] Funding text: This research was funded by grants from the NKFIH (K123975, GINOP-2.3.2-15-201600006, EFOP-3.6.2-16-2017-00006), the Momentum fellowship of the Hungarian Academy of Sciences, and the University of Debrecen. The research was financed by the Higher Education Institutional Excellence Program (NKFIH-1150-6/2019) of the Ministry of Innovation and Technology in Hungary, within the framework of the biotechnology thematic program of the University of Debrecen. Tunde Kovacs was supported by the UNKP-20-4-II-DE-68 grant. B.L.B. was supported by MOLMEDEX FUN-OMICS (GINOP-2.3.3-15-2016-00007), Debrecen Venture Catapult Program (EFOP-3.6.1-16-2016-00022), NKFIH K 129166. LA - English DB - MTMT ER - TY - JOUR AU - Sári, Zsanett Mercédesz AU - Mikó, Edit AU - Kovács, Tünde AU - Boratkó, Anita AU - Ujlaki, Gyula AU - Jankó, Laura AU - Kiss, Borbála Katalin AU - Uray (Davis), Karen L. AU - Bay, Péter TI - Indoxylsulfate, a metabolite of the microbiome, has cytostatic effects in breast cancer via activation of AHR and PXR receptors and induction of oxidative stress JF - CANCERS J2 - CANCERS VL - 12 PY - 2020 IS - 10 PG - 23 SN - 2072-6694 DO - 10.3390/cancers12102915 UR - https://m2.mtmt.hu/api/publication/31627406 ID - 31627406 N1 - Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Egyetem tér 1., Debrecen, 4032, Hungary MTA-DE Lendület Laboratory of Cellular Metabolism, Debrecen, 4032, Hungary Department of Oncology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Cited By :2 Export Date: 24 May 2021 Correspondence Address: Bai, P.; Department of Medical Chemistry, Egyetem tér 1., Hungary; email: baip@med.unideb.hu Correspondence Address: Bai, P.; MTA-DE Lendület Laboratory of Cellular MetabolismHungary; email: baip@med.unideb.hu Correspondence Address: Bai, P.; Research Center for Molecular Medicine, Hungary; email: baip@med.unideb.hu Funding details: ÚNKP-19-4-DE-79, ÚNKP-20-5-DE-96 Funding details: Funding details: ÚNKP-20-4-II-DE-68 Funding details: NKFIH-1150-6/2019 LA - English DB - MTMT ER - TY - JOUR AU - Sári, Zsanett Mercédesz AU - Mikó, Edit AU - Kovács, Tünde AU - Jankó, Laura AU - Csonka, Tamás AU - Lente, G AU - Sebő, É AU - Toth, J AU - Tóth, Dezső AU - Árkosy, Péter AU - Boratkó, Anita AU - Ujlaki, Gyula AU - Török, M AU - Kovács, Ilona AU - Szabó, Judit AU - Kiss, Borbála Katalin AU - Méhes, Gábor AU - Goedert, JJ AU - Bay, Péter TI - Indolepropionic acid, a metabolite of the microbiome, has cytostatic properties in breast cancer by activating AHR and PXR receptors and inducing oxidative stress JF - CANCERS J2 - CANCERS VL - 12 PY - 2020 IS - 9 PG - 27 SN - 2072-6694 DO - 10.3390/cancers12092411 UR - https://m2.mtmt.hu/api/publication/31408725 ID - 31408725 LA - English DB - MTMT ER - TY - JOUR AU - Jankó, Laura AU - Sári, Zsanett Mercédesz AU - Kovács, Tünde AU - Kis, Nikoletta Gréta AU - Szántó, Magdolna AU - Antal, Miklós AU - Juhász, Gábor AU - Bay, Péter TI - Silencing of PARP2 blocks autophagic degradation JF - CELLS J2 - CELLS-BASEL VL - 9 PY - 2020 IS - 2 SN - 2073-4409 DO - 10.3390/cells9020380 UR - https://m2.mtmt.hu/api/publication/31156819 ID - 31156819 N1 - Funding Agency and Grant Number: NKFIH [K123975, PD121138, KKP129797, GINOP-2.3.2-15-2016-00006]; Higher Education Institutional Excellence Programme of the Ministry of Innovation and Technology in Hungary [NKFIH-1150-6/2019]; Hungarian Academy of SciencesHungarian Academy of Sciences; [NKM-26/2019] Funding text: Our work was supported by K123975, PD121138, KKP129797, GINOP-2.3.2-15-2016-00006 from NKFIH and NKM-26/2019 and a Bolyai fellowship (to M.S.) from the Hungarian Academy of Sciences. The research was financed by the Higher Education Institutional Excellence Programme (NKFIH-1150-6/2019) of the Ministry of Innovation and Technology in Hungary, within the framework of the Biotechnology thematic programme of the University of Debrecen. LA - English DB - MTMT ER - TY - JOUR AU - Hegedűs, Csaba AU - Boros, Gábor AU - Fidrus, Eszter AU - Kis, Gréta Nikoletta AU - Antal, Miklós AU - Juhász, Tamás AU - Janka, Eszter Anna AU - Jankó, Laura AU - Paragh, György AU - Emri, Gabriella AU - Bay, Péter AU - Remenyik, Éva TI - PARP1 Inhibition Augments UVB-Mediated Mitochondrial Changes—Implications for UV-Induced DNA Repair and Photocarcinogenesis JF - CANCERS J2 - CANCERS VL - 12 PY - 2020 IS - 1 SN - 2072-6694 DO - 10.3390/cancers12010005 UR - https://m2.mtmt.hu/api/publication/31123558 ID - 31123558 N1 - 278463 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Patrik Bence AU - Csonka, Tamás AU - Kovács, Tünde AU - Sári, Zsanett Mercédesz AU - Ujlaki, Gyula AU - Sipos, Adrienn AU - Karányi, Zsolt AU - Szeőcs, Dóra AU - Hegedűs, Csaba AU - Uray (Davis), Karen L. AU - Jankó, Laura AU - Kiss, M AU - Kiss, Borbála Katalin AU - Laoui, D AU - Virág, László AU - Méhes, Gábor AU - Bay, Péter AU - Mikó, Edit TI - Lithocholic acid, a metabolite of the microbiome, increases oxidative stress in breast cancer JF - CANCERS J2 - CANCERS VL - 11 PY - 2019 IS - 9 PG - 31 SN - 2072-6694 DO - 10.3390/cancers11091255 UR - https://m2.mtmt.hu/api/publication/30774202 ID - 30774202 LA - English DB - MTMT ER - TY - JOUR AU - Márton, Judit AU - Péter, Mária AU - Balogh, Gábor AU - Bódi, Beáta AU - Vida, András AU - Szántó, Magdolna AU - Bojcsuk, Dóra AU - Jankó, Laura AU - Bhattoa Harjit, Pál AU - Gombos, Imre AU - Uray (Davis), Karen L. AU - Horváth, Ibolya AU - Török, Zsolt AU - Bálint, Bálint László AU - Papp, Zoltán AU - Vigh, László AU - Bay, Péter TI - Poly(ADP-ribose) polymerase-2 is a lipid-modulated modulator of muscular lipid homeostasis JF - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS J2 - BBA-MOL CELL BIOL L VL - 1863 PY - 2018 IS - 11 SP - 1399 EP - 1412 PG - 14 SN - 1388-1981 DO - 10.1016/j.bbalip.2018.07.013 UR - https://m2.mtmt.hu/api/publication/30385388 ID - 30385388 N1 - Cited By :4 Export Date: 20 September 2022 CODEN: BBMLF AB - There is a growing body of evidence that poly(ADP-ribose) polymerase-2 (PARP2), although originally described as a DNA repair protein, has a widespread role as a metabolic regulator. We show that the ablation of PARP2 induced characteristic changes in the lipidome. The silencing of PARP2 induced the expression of sterol regulatory element-binding protein-1 and -2 and initiated de novo cholesterol biosynthesis in skeletal muscle. Increased muscular cholesterol was shunted to muscular biosynthesis of dihydrotestosterone, an anabolic steroid. Thus, skeletal muscle fibers in PARP2(-/-) mice were stronger compared to those of their wild-type littermates. In addition, we detected changes in the dynamics of the cell membrane, suggesting that lipidome changes also affect the biophysical characteristics of the cell membrane. In in silico and wet chemistry studies, we identified lipid species that can decrease the expression of PARP2 and potentially phenocopy the genetic abruption of PARP2, including artificial steroids. In view of these observations, we propose a new role for PARP2 as a lipid-modulated regulator of lipid metabolism. LA - English DB - MTMT ER -