TY - JOUR AU - Ébert, Attila AU - Gál, Eleonóra AU - Tóth, Emese AU - Szögi, Titanilla AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Role of CFTR in diabetes‐induced pancreatic ductal fluid and HCO 3 − secretion JF - JOURNAL OF PHYSIOLOGY-LONDON J2 - J PHYSIOL-LONDON VL - 602 PY - 2024 IS - 6 SP - 1065 EP - 1083 PG - 19 SN - 0022-3751 DO - 10.1113/JP285702 UR - https://m2.mtmt.hu/api/publication/34677129 ID - 34677129 N1 - Funding Agency and Grant Number: CF-Trust CFRD-SRC [SRC 007]; National Research, Development and Innovation Office [SNN134497]; New National Excellence Program Of The Ministry Of Human Capacities [UNKP-18-4] Funding text: We are grateful for support for this study provided by the CF-Trust CFRD-SRC Grant (No.: SRC 007) and the National Research, Development and Innovation Office (SNN134497) and New National Excellence Program Of The Ministry Of Human Capacities (UNKP-18-4 to VV). LA - English DB - MTMT ER - TY - JOUR AU - Ébert, Attila AU - Gál, Eleonóra AU - Tóth, Emese AU - Hegyi, Péter AU - Venglovecz, Viktória TI - The role of CFTR in the diabetes-induced pancreatic ductal fluid and HCO3-secretion JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 9 PY - 2023 IS - Suppl 1 SP - 74 EP - 75 PG - 2 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/34053481 ID - 34053481 LA - English DB - MTMT ER - TY - JOUR AU - Marolt, Urška AU - Paradiž Leitgeb, Eva AU - Pohorec, Viljem AU - Lipovšek, Saška AU - Venglovecz, Viktória AU - Gál, Eleonóra AU - Ébert, Attila AU - Menyhárt, István AU - Potrč, Stojan AU - Gosak, Marko AU - Dolenšek, Jurij AU - Stožer, Andraž TI - Calcium imaging in intact mouse acinar cells in acute pancreas tissue slices JF - PLOS ONE J2 - PLOS ONE VL - 17 PY - 2022 IS - 6 PG - 26 SN - 1932-6203 DO - 10.1371/journal.pone.0268644 UR - https://m2.mtmt.hu/api/publication/32867137 ID - 32867137 N1 - Clinical department for abdominal and general surgery, University Medical Centre Maribor, Maribor, Slovenia Institute of Physiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia Faculty of Natural Sciences and Mathematics, University of Maribor, Maribor, Slovenia Faculty of Chemistry and Chemical Engineering, University of Maribor, Maribor, Slovenia Department of Pharmacology and Pharmacotherapy, University of Szeged, Szeged, Hungary Cited By :1 Export Date: 21 August 2022 CODEN: POLNC Correspondence Address: Marolt, U.; Clinical department for abdominal and general surgery, Slovenia; email: ursusmarolt@gmail.com Correspondence Address: Dolenšek, J.; Institute of Physiology, Slovenia; email: jurij.dolensek@um.si Correspondence Address: Stožer, A.; Institute of Physiology, Slovenia; email: andraz.stozer@um.si AB - The physiology and pathophysiology of the exocrine pancreas are in close connection to changes in intra-cellular Ca2+ concentration. Most of our knowledge is based on in vitro experiments on acinar cells or acini enzymatically isolated from their surroundings, which can alter their structure, physiology, and limit our understanding. Due to these limitations, the acute pancreas tissue slice technique was introduced almost two decades ago as a complementary approach to assess the morphology and physiology of both the endocrine and exocrine pancreas in a more conserved in situ setting. In this study, we extend previous work to functional multicellular calcium imaging on acinar cells in tissue slices. The viability and morphological characteristics of acinar cells within the tissue slice were assessed using the LIVE/DEAD assay, transmission electron microscopy, and immunofluorescence imaging. The main aim of our study was to characterize the responses of acinar cells to stimulation with acetylcholine and compare them with responses to cerulein in pancreatic tissue slices, with special emphasis on inter-cellular and inter-acinar heterogeneity and coupling. To this end, calcium imaging was performed employing confocal microscopy during stimulation with a wide range of acetylcholine concentrations and selected concentrations of cerulein. We show that various calcium oscillation parameters depend monotonically on the stimulus concentration and that the activity is rather well synchronized within acini, but not between acini. The acute pancreas tissue slice represents a viable and reliable experimental approach for the evaluation of both intra- and inter-cellular signaling characteristics of acinar cell calcium dynamics. It can be utilized to assess many cells simultaneously with a high spatiotemporal resolution, thus providing an efficient and high-yield platform for future studies of normal acinar cell biology, pathophysiology, and screening pharmacological substances. LA - English DB - MTMT ER - TY - JOUR AU - Ébert, Attila AU - Gajdos, T AU - Erdélyi, M AU - Molnár, Réka AU - Kelemen, D AU - Hegyi, P AU - Venglovecz, Viktória TI - Islet damage and regeneration in a mouse model of chronic pancreatititis JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 8 PY - 2022 IS - Suppl. 1 SP - 73 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/32843432 ID - 32843432 LA - English DB - MTMT ER - TY - JOUR AU - Gál, Eleonóra AU - Dolenšek, Jurij AU - Stožer, Andraž AU - Czakó, László AU - Ébert, Attila AU - Venglovecz, Viktória TI - Mechanisms of Post-Pancreatitis Diabetes Mellitus and Cystic Fibrosis-Related Diabetes. A Review of Preclinical Studies TS - A Review of Preclinical Studies JF - FRONTIERS IN ENDOCRINOLOGY J2 - FRONT ENDOCRINOL VL - 12 PY - 2021 SP - 715043 PG - 12 SN - 1664-2392 DO - 10.3389/fendo.2021.715043 UR - https://m2.mtmt.hu/api/publication/32246015 ID - 32246015 N1 - Journal Article; Review AB - Anatomical proximity and functional correlations between the exocrine and endocrine pancreas warrant reciprocal effects between the two parts. Inflammatory diseases of the exocrine pancreas, such as acute or chronic pancreatitis, or the presence of cystic fibrosis disrupt endocrine function, resulting in diabetes of the exocrine pancreas. Although novel mechanisms are being increasingly identified, the intra- and intercellular pathways regulating exocrine-endocrine interactions are still not fully understood, making the development of new and more effective therapies difficult. Therefore, this review sought to accumulate current knowledge regarding the pathogenesis of diabetes in acute and chronic pancreatitis, as well as cystic fibrosis. LA - English DB - MTMT ER - TY - JOUR AU - Hegyi, Péter Jenő AU - Soós, Alexandra AU - Tóth, Emese AU - Ébert, Attila AU - Venglovecz, Viktória AU - Márta, Katalin AU - Mátrai, Péter AU - Mikó, Alexandra AU - Bajor, Judit AU - Sarlós, Patrícia AU - Vincze, Áron AU - Szabó-Halász, Adrienn AU - Izbéki, Ferenc AU - Szepes, Zoltán AU - Czakó, László AU - Kovács, György AU - Papp, Mária AU - Dubravcsik, Zsolt AU - Varga, Márta AU - Hamvas, József AU - Németh, Balázs AU - Macarie, Melania AU - Ince, Ali Tüzün AU - Bordin, Dmitry S AU - Dubtsova, Elena A AU - Kiryukova, Mariya A AU - Khatkov, Igor E AU - Bideeva, Tanya AU - Mickevicius, Artautas AU - Ramírez-Maldonado, Elena AU - Sallinen, Ville AU - Erőss, Bálint Mihály AU - Pécsi, Dániel AU - Szentesi, Andrea Ildikó AU - Párniczky, Andrea AU - Tiszlavicz, László AU - Hegyi, Péter TI - Evidence for diagnosis of early chronic pancreatitis after three episodes of acute pancreatitis: a cross-sectional multicentre international study with experimental animal model JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 11 PY - 2021 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-020-80532-6 UR - https://m2.mtmt.hu/api/publication/31815619 ID - 31815619 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office [K116634, FK131864, FK124632, K128222]; Economic Development and Innovation Operative Programme Grant from the National Research, Development and Innovation Office [GINOP 2.3.2-15-2016-00048]; Human Resources Development Operational Programme Grant from the National Research, Development and Innovation Office [EFOP-3.6.2-16-2017-00006]; Hungarian Academy of Sciences [LP2014-10/2014]; Hungarian Academy of Sciences; Ministry for Innovation and Technology [UNKP-20-5] Funding text: The study was funded by Project Grants (K116634 to PH, FK131864 to AM, FK124632 to BCN and K128222 to LC), an Economic Development and Innovation Operative Programme Grant (GINOP 2.3.2-15-2016-00048 to PH) and a Human Resources Development Operational Programme Grant (EFOP-3.6.2-16-2017-00006 to PH) from the National Research, Development and Innovation Office, as well as a Momentum Grant from the Hungarian Academy of Sciences (LP2014-10/2014 to PH). Supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (to AM) and by the UNKP-20-5 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund (to AM). AB - Chronic pancreatitis (CP) is an end-stage disease with no specific therapy; therefore, an early diagnosis is of crucial importance. In this study, data from 1315 and 318 patients were analysed from acute pancreatitis (AP) and CP registries, respectively. The population from the AP registry was divided into AP (n = 983), recurrent AP (RAP, n = 270) and CP (n = 62) groups. The prevalence of CP in combination with AP, RAP2, RAP3, RAP4 and RAP5 + was 0%, 1%, 16%, 50% and 47%, respectively, suggesting that three or more episodes of AP is a strong risk factor for CP. Laboratory, imaging and clinical biomarkers highlighted that patients with RAP3 + do not show a significant difference between RAPs and CP. Data from CP registries showed 98% of patients had at least one AP and the average number of episodes was four. We mimicked the human RAPs in a mouse model and found that three or more episodes of AP cause early chronic-like morphological changes in the pancreas. We concluded that three or more attacks of AP with no morphological changes to the pancreas could be considered as early CP (ECP).The new diagnostic criteria for ECP allow the majority of CP patients to be diagnosed earlier. They can be used in hospitals with no additional costs in healthcare. LA - English DB - MTMT ER - TY - JOUR AU - Ébert, Attila AU - Tóth, Emese AU - Gajdos, T AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Diabetes increases secretory activity of pancreatic ductal epithelial cells JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 6 PY - 2020 IS - Suppl 2 SP - 91 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/31822043 ID - 31822043 LA - English DB - MTMT ER - TY - JOUR AU - Hegyi, P AU - Soós, A AU - Tóth, Emese AU - Ébert, Attila AU - Venglovecz, Viktória AU - Márta, K AU - Mátrai, P AU - Mikó, A AU - Bajor, Judit AU - Sarlós, P AU - Szabó-Halász, Adrienn AU - Izbéki, F AU - Szepes, Zoltán AU - Czakó, László AU - Kovács, G AU - Papp, M AU - Dubravcsik, Zsolt AU - Varga, M AU - Hamvas, J AU - Németh, Balázs AU - Macarie, Mq AU - Tüzün, A AU - Bordin, D AU - Kiryukova, M AU - Khatkov, I AU - Bideeva, T AU - Mickevicius, A AU - Ramírez-Maldonado, E AU - Sallinen, V AU - ErÖss, B AU - Pécsi, Dániel AU - Szentesi, Andrea Ildikó AU - Párniczky, A AU - Hegyi, Péter TI - The new definition of early chronic pancreatitis JF - PANCREATOLOGY J2 - PANCREATOLOGY VL - 20 PY - 2020 IS - Suppl. 1 SP - S56 EP - S58 PG - 3 SN - 1424-3903 DO - 10.1016/j.pan.2020.07.071 UR - https://m2.mtmt.hu/api/publication/31822032 ID - 31822032 LA - English DB - MTMT ER - TY - JOUR AU - Hegyi, P AU - Soós, A AU - Tóth, Emese AU - Ébert, Attila AU - Venglovecz, Viktória AU - Márta, K AU - Mikó, A AU - Bajor, J AU - Sarlós, P AU - Vincze, Á AU - Szabó-Halász, Adrienn AU - Izbéki, F AU - Szepes, Zoltán AU - Czakó, László AU - Kovács, L. Gábor AU - Papp, Mária AU - Dubravcsik, Zsolt AU - Varga, M AU - Hamvas, J AU - Németh, Balázs AU - Erőss, B AU - Pécsi, D AU - Szentesi, Andrea Ildikó AU - Párniczky, A AU - Hegyi, Péter TI - The new definition of early chronic pancreatitis JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 6 PY - 2020 IS - Suppl 2 SP - 54 EP - 54 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/31784556 ID - 31784556 LA - English DB - MTMT ER - TY - CHAP AU - Gál, Eleonóra AU - Dolenšek, J AU - Stožer, A AU - Pohorec, V AU - Ébert, Attila AU - Venglovecz, Viktória ED - Rakonczay, Zoltán ED - Kiss, Lóránd TI - Development of a new method for the examination of pancreatic ducts T2 - Proceedings of the EFOP-3.6.2-16-2017-00006 (LIVE LONGER) project PB - University of Szeged CY - Szeged SN - 9789633067642 PY - 2020 SP - 16 UR - https://m2.mtmt.hu/api/publication/31641281 ID - 31641281 LA - English DB - MTMT ER -