@article{MTMT:33708483,
	title = {Prolonged activity of the transposase helper may raise safety concerns during DNA transposon-based gene therapy},
	url = {https://m2.mtmt.hu/api/publication/33708483},
	author = {Imre, Gergely and Takács, Bertalan Vilmos and Czipa, Erik and Drubi, Andrea and Jaksa, Gábor and Latinovics, Dóra and Nagy, Andrea and Karkas, Réka and Hudoba, Liza and Vásárhelyi, Bálint Márk and Pankotai-Bodó, Gabriella and Blastyák, András and Hegedűs, Zoltán and Germán, Péter and Bálint, Balázs and Ahmed Abdullah, Khaldoon Sadiq and Kopasz, Anna Georgina and Kovács, Anita Kármen and Nagy, László and Sükösd, Farkas and Pintér, Lajos and Rülicke, Thomas and Barta, Endre and Nagy, István and Haracska, Lajos and Mátés, Lajos},
	doi = {10.1016/j.omtm.2023.03.003},
	journal-iso = {MOL THER-METH CLIN D},
	journal = {MOLECULAR THERAPY-METHODS AND CLINICAL DEVELOPMENT},
	volume = {29},
	unique-id = {33708483},
	year = {2023},
	eissn = {2329-0501},
	pages = {145-159},
	orcid-numbers = {Vásárhelyi, Bálint Márk/0000-0003-1782-8691; Kovács, Anita Kármen/0000-0001-9805-1647}
}

@article{MTMT:31288128,
	title = {Stability Test of PACAP in Eye Drops},
	url = {https://m2.mtmt.hu/api/publication/31288128},
	author = {Kovács, Anita Kármen and Atlasz, Tamás and Werling, Dóra and Szabó, Edina and Reglődi, Dóra and Tóth, Gábor},
	doi = {10.1007/s12031-020-01532-9},
	journal-iso = {J MOL NEUROSCI},
	journal = {JOURNAL OF MOLECULAR NEUROSCIENCE},
	volume = {71},
	unique-id = {31288128},
	issn = {0895-8696},
	abstract = {PACAP is a neuropeptide with widespread distribution and diverse biological functions. It has strong cytoprotective effects mediated mainly through specific PAC1 receptors. Experimental data show protective effects of PACAP in the retina and cornea in several pathological conditions. Although intravitreal injections are a common practice in some ocular diseases, delivery of therapeutic agents in the form of eye drops would be more convenient and would lead to fewer side effects. We have previously shown that PACAP, in the form of eye drops, is able to pass through the ocular barriers and can exert retinoprotective effects. As eye drops represent a promising form of administration of PACAP in ocular diseases, it is important to investigate the stability of PACAP in solutions used in eye drops. In this study, the stability of PACAP1-27 and PACAP1-38 in eye drops was measured in four common media and a commercially available artificial tear solution at both room temperature and +4 °C. Mass spectrometry results show that the highest stability was gained with PACAP1-38 in water and 0.9% saline solution at +4 °C, representing 80–90% drug persistence after 2 weeks. PACAP1-38 in the artificial tear showed very fast degradation at room temperature, but was stable at +4 °C. In summary, PACAP1-38 has higher stability than PACAP1-27, with highest stability at +4 °C in water solution, but both peptides in each medium can be stored for relatively longer periods without significant degradation. These data can provide reference for future therapeutic use of PACAP in eye drops.},
	year = {2021},
	eissn = {1559-1166},
	pages = {1567-1574},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647; Atlasz, Tamás/0000-0002-8112-8633; Werling, Dóra/0000-0003-0796-7997; Szabó, Edina/0000-0002-0653-9547; Reglődi, Dóra/0000-0001-5481-5529; Tóth, Gábor/0000-0002-3604-4385}
}

@article{MTMT:31385279,
	title = {Imidazo[1,2-b]pyrazole-7-Carboxamide Derivative Induces Differentiation-Coupled Apoptosis of Immature Myeloid Cells Such as Acute Myeloid Leukemia and Myeloid-Derived Suppressor Cells},
	url = {https://m2.mtmt.hu/api/publication/31385279},
	author = {Kotogány, Edit and Balog, József Ágoston and Nagy, Lajos I. and Alföldi, Róbert and Bertagnolo, Valeria and Brugnoli, Federica and Demjén, András and Kovács, Anita Kármen and Batár, Péter and Mezei, Gabriella and Szabó, Renáta and Kanizsai, Iván and Varga, Csaba and Puskás, László and Szebeni, Gábor},
	doi = {10.3390/ijms21145135},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {21},
	unique-id = {31385279},
	issn = {1661-6596},
	year = {2020},
	eissn = {1422-0067},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647; Varga, Csaba/0000-0002-2678-665X; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:31143380,
	title = {Single Cell Mass Cytometry Revealed the Immunomodulatory Effect of Cisplatin Via Downregulation of Splenic CD44+, IL-17A+ MDSCs and Promotion of Circulating IFN-γ+ Myeloid Cells in the 4T1 Metastatic Breast Cancer Model.},
	url = {https://m2.mtmt.hu/api/publication/31143380},
	author = {Balog, József Ágoston and Hackler, László and Kovács, Anita Kármen and Neuperger, Patricia and Alföldi, Róbert and Nagy, Lajos István and Puskás, László and Szebeni, Gábor},
	doi = {10.3390/ijms21010170},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {21},
	unique-id = {31143380},
	issn = {1661-6596},
	abstract = {The treatment of metastatic breast cancer remained a challenge despite the recent breakthrough in the immunotherapy regimens. Here, we addressed the multidimensional immunophenotyping of 4T1 metastatic breast cancer by the state-of-the-art single cell mass cytometry (CyTOF). We determined the dose and time dependent cytotoxicity of cisplatin on 4T1 cells by the xCelligence real-time electronic sensing assay. Cisplatin treatment reduced tumor growth, number of lung metastasis, and the splenomegaly of 4T1 tumor bearing mice. We showed that cisplatin inhibited the tumor stroma formation, the polarization of carcinoma-associated fibroblasts by the diminished proteolytic activity of fibroblast activating protein. The CyTOF analysis revealed the emergence of CD11b+/Gr-1+/CD44+ or CD11b+/Gr-1+/IL-17A+ myeloid-derived suppressor cells (MDSCs) and the absence of B220+ or CD62L+ B-cells, the CD62L+/CD4+ and CD62L+/CD8+ T-cells in the spleen of advanced cancer. We could show the immunomodulatory effect of cisplatin via the suppression of splenic MDSCs and via the promotion of peripheral IFN-γ+ myeloid cells. Our data could support the use of low dose chemotherapy with cisplatin as an immunomodulatory agent for metastatic triple negative breast cancer.},
	keywords = {immunophenotyping; Metastatic breast cancer; Myeloid-derived suppressor cells; single cell mass cytometry},
	year = {2020},
	eissn = {1422-0067},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647; Szebeni, Gábor/0000-0002-6998-5632}
}

@mastersthesis{MTMT:30806726,
	title = {Optimized synthesis routes and biological application of N-peptide-6-amino-D-luciferin conjugates},
	url = {https://m2.mtmt.hu/api/publication/30806726},
	author = {Kovács, Anita Kármen},
	doi = {10.14232/phd.10082},
	publisher = {SZTE},
	unique-id = {30806726},
	year = {2019},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647}
}

@article{MTMT:30382079,
	title = {Synthesis of N-Peptide-6-Amino-d-Luciferin Conjugates with Optimized Fragment Condensation Strategy},
	url = {https://m2.mtmt.hu/api/publication/30382079},
	author = {Kovács, Anita Kármen and Hegyes, P. and Szebeni, Gábor and Bogár, K. and Puskás, László and Tóth, Gábor},
	doi = {10.1007/s10989-018-9768-8},
	journal-iso = {INT J PEPT RES THER},
	journal = {INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS},
	volume = {25},
	unique-id = {30382079},
	issn = {1573-3149},
	abstract = {Abstract: The synthesis of peptide-luciferin conjugates has a pivotal role in the development of bioluminescent detection systems that are based on the determination of protease enzyme activity. This work describes the optimized synthesis of an N-peptide-6-amino-d-luciferin conjugate (Fmoc-Gly-Pro-6-amino-d-luciferin) with a simple fragment condensation method in adequate yields. Fmoc-Gly-Pro-6-amino-d-luciferin was produced from a previously synthesized Fmoc-Gly-Pro-OH and also previously synthesized 6-amino-2-cyanobenzothiazole with an optimized method, to which conjugate cysteine was added in an also improved way. The resulting conjugate was successfully used in a bioluminescent system, in vitro, demonstrating the applicability of the method. Graphical Abstract: [Figure not available: see fulltext.]. © 2018, Springer Nature B.V.},
	keywords = {CONJUGATE; BIOLUMINESCENCE; protease activity; aminoluciferin; Fragment condensation},
	year = {2019},
	eissn = {1573-3904},
	pages = {1209-1215},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647; Szebeni, Gábor/0000-0002-6998-5632; Tóth, Gábor/0000-0002-3604-4385}
}

@article{MTMT:3389411,
	title = {Lewis Acid-Catalyzed Diastereoselective Synthesis of Multisubstituted N-Acylaziridine-2-carboxamides from 2H-Azirines via Joullie-Ugi Three-Component Reaction},
	url = {https://m2.mtmt.hu/api/publication/3389411},
	author = {Angyal, Anikó and Demjén, András and Wéber, Edit and Kovács, Anita Kármen and Wölfling, János and Puskás, László and Kanizsai, Iván},
	doi = {10.1021/acs.joc.7b03189},
	journal-iso = {J ORG CHEM},
	journal = {JOURNAL OF ORGANIC CHEMISTRY},
	volume = {83},
	unique-id = {3389411},
	issn = {0022-3263},
	abstract = {A ZnCl2-catalyzed diastereoselective Joullie Ugi three-component reaction from 2H-azirines, isocyanides, and carboxylic acids was established. The protocol allows the preparation of highly and diversely functionalized N-acylaziridine-2-carboxamide derivatives in up to 82% isolated yields. Moreover, the applicability of N-acylaziridines is demonstrated through a variety of transformations.},
	keywords = {ASYMMETRIC-SYNTHESIS; CYSTEINE PROTEASES; enantioselective synthesis; MULTICOMPONENT REACTIONS; N-ACYLAZIRIDINES; 3+2 CYCLOADDITION; CARBOXYLIC ESTERS; AZIRIDINYL PEPTIDES; 2,4-DISUBSTITUTED OXAZOLES; SUBSTITUTED PROLYL PEPTIDES},
	year = {2018},
	eissn = {1520-6904},
	pages = {3570-3581},
	orcid-numbers = {Wéber, Edit/0000-0002-5904-0619; Kovács, Anita Kármen/0000-0001-9805-1647; Wölfling, János/0000-0002-3037-309X}
}

@article{MTMT:3361945,
	title = {Synthesis of N-peptide-6-amino-D-luciferin Conjugates},
	url = {https://m2.mtmt.hu/api/publication/3361945},
	author = {Kovács, Anita Kármen and Hegyes, Péter and Szebeni, Gábor and Nagy, Lajos I and Puskás, László and Tóth, Gábor},
	doi = {10.3389/fchem.2018.00120},
	journal-iso = {FRONT CHEM},
	journal = {FRONTIERS IN CHEMISTRY},
	volume = {6},
	unique-id = {3361945},
	issn = {2296-2646},
	abstract = {A general strategy for the synthesis of N-peptide-6-amino-D-luciferin conjugates has been developed. The applicability of the strategy was demonstrated with the preparation of a known substrate, N-Z-Asp-Glu-Val-Asp-6-amino-D-luciferin (N-Z-DEVD-aLuc). N-Z-DEVD-aLuc was obtained via a hybrid liquid/solid phase synthesis method, in which the appropriately protected C-terminal amino acid was coupled to 6-amino-2-cyanobenzothiazole and the resulting conjugate was reacted with D-cysteine in order to get the protected amino acid-6-amino-D-luciferin conjugate, which was then attached to resin. The resulting loaded resin was used for the solid-phase synthesis of the desired N-peptide-6-amino-D-luciferin conjugate without difficulties, which was then attested with NMR spectroscopy and LC-MS, and successfully tested in a bioluminescent system.},
	keywords = {MODEL; APOPTOSIS; SUBSTRATE; ANALOGS; PROTEASES; CONJUGATE; REAGENT; ASSAYS; BIOLUMINESCENCE; solid-phase peptide synthesis; protease activity; firefly luciferase; aminoluciferin},
	year = {2018},
	eissn = {2296-2646},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647; Szebeni, Gábor/0000-0002-6998-5632; Tóth, Gábor/0000-0002-3604-4385}
}

@CONFERENCE{MTMT:3284935,
	title = {Comparison of Fmoc-, Boc- and fragment condensation strategies in the synthesis of peptide-6-amino-D-luciferin conjugates},
	url = {https://m2.mtmt.hu/api/publication/3284935},
	author = {Kovács, Anita Kármen and Hagyes, P and Szebeni, Gábor and Puskas, LG and Toth, GK},
	booktitle = {12th Australian Peptide Conference},
	unique-id = {3284935},
	year = {2017},
	pages = {89},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647; Szebeni, Gábor/0000-0002-6998-5632}
}

@CONFERENCE{MTMT:3184498,
	title = {Novel synthesis methods of peptide-t-amino-D-luciferin conjugates for protease activity detection},
	url = {https://m2.mtmt.hu/api/publication/3184498},
	author = {Kovács, Anita Kármen and Hegyes, P and Szebeni, Gábor and Puskas, LG and Toth, GK},
	booktitle = {23rd Young Research Fellow Meeting},
	unique-id = {3184498},
	year = {2016},
	orcid-numbers = {Kovács, Anita Kármen/0000-0001-9805-1647; Szebeni, Gábor/0000-0002-6998-5632}
}