TY - JOUR AU - Tököli, Attila AU - Bodnár, Brigitta AU - Bogár, Ferenc AU - Paragi, Gábor AU - Hetényi, Anasztázia AU - Bartus, Éva AU - Wéber, Edit AU - Hegedüs, Zsófia AU - Szabó, Zoltán AU - Kecskeméti, Gábor AU - Szakonyi, Gerda AU - Martinek, Tamás TI - Structural Adaptation of the Single-Stranded DNA-Binding Protein C-Terminal to DNA Metabolizing Partners Guides Inhibitor Design JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 4 PG - 17 SN - 1999-4923 DO - 10.3390/pharmaceutics15041032 UR - https://m2.mtmt.hu/api/publication/33712712 ID - 33712712 N1 - Department of Medical Chemistry, University of Szeged, Szeged, H6720, Hungary ELKH-SZTE Biomimetic Systems Research Group, Eötvös Loránd Research Network (ELKH), Szeged, H6720, Hungary Institute of Physics, University of Pécs, Pécs, H7624, Hungary Department of Theoretical Physics, University of Szeged, Szeged, H6720, Hungary Institute of Pharmaceutical Analysis, University of Szeged, Szeged, H6720, Hungary Export Date: 8 September 2023 Correspondence Address: Martinek, T.A.; Department of Medical Chemistry, Hungary; email: martinek.tamas@med.u-szeged.hu AB - Single-stranded DNA-binding protein (SSB) is a bacterial interaction hub and an appealing target for antimicrobial therapy. Understanding the structural adaptation of the disordered SSB C-terminus (SSB-Ct) to DNA metabolizing enzymes (e.g., ExoI and RecO) is essential for designing high-affinity SSB mimetic inhibitors. Molecular dynamics simulations revealed the transient interactions of SSB-Ct with two hot spots on ExoI and RecO. The residual flexibility of the peptide–protein complexes allows adaptive molecular recognition. Scanning with non-canonical amino acids revealed that modifications at both termini of SSB-Ct could increase the affinity, supporting the two-hot-spot binding model. Combining unnatural amino acid substitutions on both segments of the peptide resulted in enthalpy-enhanced affinity, accompanied by enthalpy–entropy compensation, as determined by isothermal calorimetry. NMR data and molecular modeling confirmed the reduced flexibility of the improved affinity complexes. Our results highlight that the SSB-Ct mimetics bind to the DNA metabolizing targets through the hot spots, interacting with both of segments of the ligands. LA - English DB - MTMT ER - TY - JOUR AU - Hetényi, Anasztázia AU - Imre, Norbert AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Fehérje méretű molekulák humán sejtekbe juttatása lipid-raft mediált endocitózissal JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 45 PY - 2021 IS - 4 SP - 67 EP - 83 PG - 17 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/32570862 ID - 32570862 N1 - Nincs jelölve levelező szerzőség a közleményen. (BÉ SZTE admin5) LA - Hungarian DB - MTMT ER - TY - PAT AU - Imre, Norbert AU - Martinek, Tamás AU - Hetényi, Anasztázia AU - Bodnár, Brigitta AU - Monostori, Eva AU - Czibula, Agnes AU - Szabo, Eniko AU - Deli, Mária Anna AU - Horvath, Peter TI - Endocytosis routing sequence peptide for cell delivery systems PY - 2020 UR - https://m2.mtmt.hu/api/publication/32024174 ID - 32024174 AB - The present invention relates to cell delivery systems. The present invention specifically relates to new methods of intracellular delivery by endocytosis routing sequence peptides having the sequence of WYKYV or analogs thereof, by caveloar/lipid raft-mediated endocytosis and uses of such peptides. The invention also relates to conjugates comprising said peptides and uses thereof in therapies wherein intracellular delivery of a therapeutically active mol. is required. LA - English DB - MTMT ER - TY - JOUR AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Routing Nanomolar Protein Cargoes to Lipid Raft‐Mediated/Caveolar Endocytosis through a Ganglioside GM1‐Specific Recognition Tag JF - ADVANCED SCIENCE J2 - ADV SCI VL - 7 PY - 2020 IS - 4 PG - 10 SN - 2198-3844 DO - 10.1002/advs.201902621 UR - https://m2.mtmt.hu/api/publication/31126947 ID - 31126947 N1 - Funding text: This research was funded by the National Research, Development and Innovation Office of Hungary, grant number GINOP-2.2.1-15-2016-00007, the Hungarian Ministry of Innovation and Technology, TUDFO/47138-1/2019-ITM, and Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG). T.A.M. acknowledges support from the Hungarian Academy of Sciences LENDULET-Foldamer. P.H. acknowledges support from the Finnish TEKES FiDiPro Fellow Grant 40294/13, the Hungarian Academy of Sciences LENDULET-Biomag. LA - English DB - MTMT ER - TY - GEN AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, A. Mária AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - IgG bejuttatása specifikus GM1 gangliozid felismerő szekvenciával PY - 2019 UR - https://m2.mtmt.hu/api/publication/31624665 ID - 31624665 LA - Hungarian DB - MTMT ER - TY - THES AU - Bodnár, Brigitta TI - Synthesis of monosaccharide and nucleoside conjugates of estrone derivatives PB - Szegedi Tudományegyetem (SZTE) PY - 2018 SP - 56 DO - 10.14232/phd.9761 UR - https://m2.mtmt.hu/api/publication/3402058 ID - 3402058 LA - English DB - MTMT ER - TY - GEN AU - Bodnár, Brigitta AU - Kovács, Lajos AU - Zoltán, Kupihár TI - Synthesis of 5'-azidonucleosides as building blocks for the preparation of biologically active bioconjugates CY - 2017. augusztus 28-29. PY - 2017 UR - https://m2.mtmt.hu/api/publication/3337576 ID - 3337576 LA - English DB - MTMT ER - TY - GEN AU - Bodnár, Brigitta TI - 5'-Azidonukleozidok előállítása CY - 2017. március 31.-április 02. PY - 2017 UR - https://m2.mtmt.hu/api/publication/3337573 ID - 3337573 LA - Hungarian DB - MTMT ER - TY - GEN AU - Bodnár, Brigitta AU - Kovács, Lajos AU - Zoltán, Kupihár TI - The synthesis of 5'-azido-5'-deoxynucleosides and homologues thereof CY - 2017. május 31.-június 02. PY - 2017 UR - https://m2.mtmt.hu/api/publication/3337568 ID - 3337568 LA - English DB - MTMT ER - TY - PAT AU - Kupihár, Zoltán AU - Bodnár, Brigitta AU - Kovács, Lajos TI - 5'-linkerrel módosított nukleozidok CY - Country:10001(1) PY - 2017 UR - https://m2.mtmt.hu/api/publication/3327529 ID - 3327529 LA - Hungarian DB - MTMT ER -