TY - JOUR AU - Rónavári, Andrea AU - Ochirkhuyag, Altantuya AU - Igaz, Nóra AU - Szerencsés, Bettina AU - Ballai, Gergő AU - Huliák, Ildikó AU - Bocz, Csenge AU - Kovács, Ákos AU - Pfeiffer, Ilona AU - Csontné Kiricsi, Mónika AU - Kónya, Zoltán TI - Preparation, characterization and in vitro evaluation of the antimicrobial and antitumor activity of MnOx nanoparticles JF - COLLOIDS AND SURFACES A : PHYSICOCHEMICAL AND ENGINEERING ASPECTS J2 - COLLOID SURFACE A VL - 688 PY - 2024 SN - 0927-7757 DO - 10.1016/j.colsurfa.2024.133528 UR - https://m2.mtmt.hu/api/publication/34687262 ID - 34687262 LA - English DB - MTMT ER - TY - CONF AU - Adamecz, Dóra Izabella AU - Petra, Bicskei AU - Árva, Hédi AU - Igaz, Nóra AU - Veres, Éva AU - Rónavári, Andrea AU - Gácser, Attila AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika TI - The possible modulatory effects of metal nanoparticles on macrophage polarization in co-culture T2 - Abstracts for the 47th FEBS Congress PY - 2023 UR - https://m2.mtmt.hu/api/publication/34154600 ID - 34154600 LA - English DB - MTMT ER - TY - CONF AU - Igaz, Nóra AU - Szőke, Krisztina AU - Bocz, Csenge AU - Kovács, Dávid AU - Rónavári, Andrea AU - Szabó, Emilia Rita AU - Polanek, Róbert AU - Buhala, Andrea AU - Vizler, Csaba AU - Tiszlavicz, László AU - Rázga, Zsolt AU - Hideghéty, Katalin AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika TI - Radiosensitizing effect of metal nanoparticles in combination with histone deacetylase inhibitors T2 - FAMÉ 2023 PY - 2023 SP - 75 EP - 76 PG - 2 UR - https://m2.mtmt.hu/api/publication/34154565 ID - 34154565 LA - English DB - MTMT ER - TY - JOUR AU - Szatmári, Orsolya AU - Nagy-Mikó, Bence AU - Györkei, Ádám AU - Varga, Dániel AU - H. Kovács, Bálint Barna AU - Igaz, Nóra AU - Bognár, Bence AU - Rázga, Zsolt AU - Nagy, Gábor AU - Zsindely, Nóra AU - Bodai, László AU - Papp, Balázs AU - Erdélyi, Miklós AU - Csontné Kiricsi, Mónika AU - Blastyák, András AU - Collart, Martine A AU - Boros, Imre Miklós AU - Villanyi, Zoltan TI - Phase-separated ribosome-nascent chain complexes in genotoxic stress response JF - RNA-A PUBLICATION OF THE RNA SOCIETY J2 - RNA VL - 29 PY - 2023 IS - 10 SP - 1557 EP - 1574 PG - 18 SN - 1355-8382 DO - 10.1261/rna.079755.123 UR - https://m2.mtmt.hu/api/publication/34067381 ID - 34067381 N1 - Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary Section for Physiology and Cell Biology, Department of Biosciences, University of Oslo, Oslo, 0316, Norway Department of Optics and Quantum Electronics, University of Szeged, Szeged, 6720, Hungary Department of Pathology, Faculty of Medicine, University of Szeged, Szeged, 6720, Hungary Institute of Genetics, Biological Research Centre, Szeged, 6726, Hungary Department of Microbiology and Molecular Medicine, Institute of Genetics and Genomics Geneva, Faculty of Medicine, University of Geneva, Geneva 4, 1211, Switzerland Cited By :1 Export Date: 12 December 2023 CODEN: RNARF Correspondence Address: Villányi, Z.; Department of Biochemistry and Molecular Biology, Hungary; email: villanyi.zoltan@bio.u-szeged.hu Chemicals/CAS: 1,6 hexanediol, 629-11-8; DNA helicase; edetic acid, 150-43-6, 60-00-4; ribonuclease, 59794-03-5, 9001-99-4; transcriptional regulator ATRX; Werner syndrome ATP dependent helicase; RNA, 63231-63-0; Edetic Acid; RecQ Helicases; Ribonucleoproteins; RNA; Saccharomyces cerevisiae Proteins; SGS1 protein, S cerevisiae Funding details: 31003A_172999, NTP-NFTÖ-20-B-0354 Funding details: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNF Funding details: Magyar Tudományos Akadémia, MTA, BO/00878/19/8, BO/902/19, TKP2021-NVA-19 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding text 1: We are grateful to Dr. Balázs Vedelek and Dr. Zsuzsa Sarkadi for valuable discussions. We thank Jawad Iqbal, Elvira Czvik, Zita Kóra, and Edina Pataki for technical assistance. We are grateful to Blanka Léhy for the graphical abstract. This work was supported by grants GINOP-2.3.2-15-2016-00020 and GINOP-2.3.2-15-2016-00038, as well as by NKFI-K 142961 (Z.V.), ÚNKP-21-5-595-SZTE (Z.V.), and ÚNKP-20-5-SZTE-655 (M.K.) from the Hungarian National Research, Development and Innovation Office. Further support was provided by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/902/19 for Z.V. and BO/00878/19/8 for M.K.). Superresolu-tion dSTORM experiments and their evaluation were funded by the Hungarian National Research, Development and Innovation Office (TKP2021-NVA-19), Hungarian Brain Research Program (2017-1.2.1-NKP-2017-00002) awarded to M.E., and NTP-NFTÖ-20-B-0354 awarded to D.V., as well as grant 31003A_172999 from the Swiss National Science Foundation awarded to M.A.C. AB - Assemblysomes are EDTA- and RNase-resistant ribonucleoprotein (RNP) complexes of paused ribosomes with protruding nascent polypeptide chains. They have been described in yeast and human cells for the proteasome subunit Rpt1, and the disordered N-terminal part of the nascent chain was found to be indispensable for the accumulation of the Rpt1-RNP into assemblysomes. Motivated by this, to find other assemblysome-associated RNPs we used bioinformatics to rank subunits of Saccharomyces cerevisiae protein complexes according to their N-terminal disorder propensity. The results revealed that gene products involved in DNA repair are enriched among the top candidates. The Sgs1 DNA helicase was chosen for experimental validation. We found that indeed nascent chains of Sgs1 form EDTA-resistant RNP condensates, assemblysomes by definition. Moreover, upon exposure to UV, SGS1 mRNA shifted from assemblysomes to polysomes, suggesting that external stimuli are regulators of assemblysome dynamics. We extended our studies to human cell lines. The BLM helicase, ortholog of yeast Sgs1, was identified upon sequencing assemblysome-associated RNAs from the MCF7 human breast cancer cell line, and mRNAs encoding DNA repair proteins were overall enriched. Using the radiation-resistant A549 cell line, we observed by transmission electron microscopy that 1,6-hexanediol, an agent known to disrupt phase-separated condensates, depletes ring ribosome structures compatible with assemblysomes from the cytoplasm of cells and makes the cells more sensitive to X-ray treatment. Taken together these findings suggest that assemblysomes may be a component of the DNA damage response from yeast to human. LA - English DB - MTMT ER - TY - JOUR AU - Petrasheuskaya, Tatsiana AU - Kovács, Ferenc AU - Igaz, Nóra AU - Rónavári, Andrea AU - Hajdu, Bálint AU - Nagyné Bereczki, Laura AU - May, Nóra Veronika AU - Spengler, Gabriella AU - Gyurcsik, Béla AU - Csontné Kiricsi, Mónika AU - Nagyné Frank, Éva AU - Enyedy, Éva Anna TI - Estradiol-Based Salicylaldehyde (Thio)semicarbazones and Their Copper Complexes with Anticancer, Antibacterial and Antioxidant Activities JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 1 PG - 26 SN - 1420-3049 DO - 10.3390/molecules28010054 UR - https://m2.mtmt.hu/api/publication/33416616 ID - 33416616 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office-NKFIA (Hungary) [TKP-2021-EGA-32]; OTKA [K124544]; 'Lendulet' Programme ELKH [LP2019-6/2019]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/00158/22/5]; New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund [UNKP-22-5-SZTE-588] Funding text: This work was supported by the National Research, Development and Innovation Office-NKFIA (Hungary) through project TKP-2021-EGA-32 and OTKA grant K124544. The support of the 'Lendulet' Programme (ELKH, LP2019-6/2019) is also acknowledged. G.S. was supported by the Janos Bolyai Research Scholarship (BO/00158/22/5) of the Hungarian Academy of Sciences and by the UNKP-22-5-SZTE-588 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. AB - A series of novel estradiol-based salicylaldehyde (thio)semicarbazones ((T)SCs) bearing (O,N,S) and (O,N,O) donor sets and their Cu(II) complexes were developed and characterized in detail by 1H and ¹³C nuclear magnetic resonance spectroscopy, UV–visible and electron paramagnetic resonance spectroscopy, electrospray ionization mass spectrometry and elemental analysis. The structure of the Cu(II)-estradiol-semicarbazone complex was revealed by X-ray crystallography. Proton dissociation constants of the ligands and stability constants of the metal complexes were determined in 30% (v/v) DMSO/H2O. Estradiol-(T)SCs form mono-ligand complexes with Cu(II) ions and exhibit high stability with the exception of estradiol-SC. The Cu(II) complexes of estradiol-TSC and its N,N-dimethyl derivative displayed the highest cytotoxicity among the tested compounds in MCF-7, MCF-7 KCR, DU-145, and A549 cancer cells. The complexes do not damage DNA according to both in vitro cell-free and cellular assays. All the Cu(II)-TSC complexes revealed significant activity against the Gram-positive Staphylococcus aureus bacteria strain. Estradiol-TSCs showed efficient antioxidant activity, which was decreased by complexation with Cu(II) ions. The exchange of estrone moiety to estradiol did not result in significant changes to physico-chemical and biological properties. LA - English DB - MTMT ER - TY - JOUR AU - Igaz, Nóra AU - Bélteky, Péter AU - Kovács, Dávid AU - Papp, Csaba Gergő AU - Rónavári, Andrea AU - Szabó, Diána AU - Gácser, Attila AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika TI - Functionalized Mesoporous Silica Nanoparticles for Drug-Delivery to Multidrug-Resistant Cancer Cells JF - INTERNATIONAL JOURNAL OF NANOMEDICINE J2 - INT J NANOMED VL - 17 PY - 2022 SP - 3079 EP - 3096 PG - 18 SN - 1176-9114 DO - 10.2147/IJN.S363952 UR - https://m2.mtmt.hu/api/publication/33003489 ID - 33003489 N1 - Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary Department of Applied and Environmental Chemistry, University of Szeged, Szeged, Hungary Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur, Inserm, CNRS, Valbonne, France HCEMM-USZ Fungal Pathogens Research Group, Department of Microbiology, University of Szeged, Szeged, Hungary Department of Oto-Rhino-Laryngology and Head & Neck Surgery, Szeged, Hungary Eötvös Loránd Research Network, Reaction Kinetics and Surface Chemistry Research Group, Szeged, Hungary Cited By :8 Export Date: 27 February 2024 Correspondence Address: Kónya, Z.; Department of Applied and Environmental Chemistry, Rerrich square 1, Hungary; email: konya@chem.u-szeged.hu Correspondence Address: Kiricsi, M.; Department of Biochemistry and Molecular Biology, Közép fasor 52, Hungary; email: kiricsim@bio.u-szeged.hu AB - Background: Multidrug resistance is a common reason behind the failure of chemotherapy. Even if the therapy is effective, serious adverse effects might develop due to the low specificity and selectivity of antineoplastic agents. Mesoporous silica nanoparticles (MSNs) are promising materials for tumor-targeting and drug-delivery due to their small size, relatively inert nature, and extremely large specific surfaces that can be functionalized by therapeutic and targeting entities. We aimed to create a fluorescently labeled MSN-based drug-delivery system and investigate their internalization and drug-releasing capability in drug-sensitive MCF-7 and P-glycoprotein-overexpressing multidrug-resistant MCF-7 KCR cancer cells.Methods and Results: To track the uptake and subcellular distribution of MSNs, particles with covalently coupled red fluorescent Rhodamine B (RhoB) were produced (RhoB@MSNs). Both MCF-7 and MCF-7 KCR cells accumulated a significant amount of RhoB@MSNs. The intracellular RhoB@MSN concentrations did not differ between sensitive and multidrug-resistant cells and were kept at the same level even after cessation of RhoB@MSN exposure. Although most RhoB@MSNs resided in the cytoplasm, significantly more RhoB@MSNs co-localized with lysosomes in multidrug-resistant cells compared to sensitive counterparts. To examine the drug-delivery capability of these particles, RhoB@Rho123@MSNs were established, where RhoB-functionalized nanoparticles carried green fluorescent Rhodamine 123 (Rho123) -a P-glycoprotein substrate - as cargo within mesopores. Significantly higher Rho123 fluorescence intensity was detected in RhoB@Rho123@MSN-treated multidrug-resistant cells than in free Rho123-exposed counterparts. The exceptional drug-delivery potential of MSNs was further verified using Mitomycin C (MMC)-loaded RhoB@MSNs (RhoB@MMC@MSNs). Exposures to RhoB@MMC@MSNs significantly decreased the viability not only of drug-sensitive but of multidrug-resistant cells and the elimination of MDR cells was significantly more robust than upon free MMC treatments.Conclusion: The efficient delivery of Rho123 and MMC to multidrug-resistant cells via MSNs, the amplified and presumably prolonged intracellular drug concentration, and the consequently enhanced cytotoxic effects envision the enormous potential of MSNs to defeat multidrug-resistant cancer. LA - English DB - MTMT ER - TY - GEN AU - Szatmári, Orsolya AU - Györkei, Á AU - Varga, Dániel AU - H. Kovács, Bálint Barna AU - Igaz, Nóra AU - Német, K AU - Bagi, N AU - Nagy-Mikó, B AU - Balogh, D AU - Rázga, Zsolt AU - Erdélyi, Miklós AU - Papp, B AU - Csontné Kiricsi, Mónika AU - Blastyák, A AU - Collart, MA AU - Boros, Imre Miklós AU - Villanyi, Zoltan TI - Phase separated ribosome nascent chain complexes paused in translation are capable to continue expression of proteins playing role in genotoxic stress response upon DNA damage PY - 2022 DO - 10.1101/2022.03.16.484567 UR - https://m2.mtmt.hu/api/publication/32783378 ID - 32783378 LA - English DB - MTMT ER - TY - JOUR AU - Vadovics, Máté AU - Ho, Jemima AU - Igaz, Nóra AU - Alföldi, Róbert AU - Rakk, Dávid AU - Veres, Éva AU - Szücs, Balázs AU - Horváth, Márton AU - Tóth, Renáta AU - Szűcs, Attila AU - Csibi, Andrea AU - Horváth, Péter AU - Tiszlavicz, László AU - Vágvölgyi, Csaba AU - Nosanchuk, Joshua D. AU - Szekeres, András AU - Csontné Kiricsi, Mónika AU - Henley-Smith, Rhonda AU - Moyes, David L. AU - Thavaraj, Selvam AU - Brown, Rhys AU - Puskás, László AU - Naglik, Julian R. AU - Gácser, Attila TI - Candida albicans Enhances the Progression of Oral Squamous Cell Carcinoma In Vitro and In Vivo JF - MBIO J2 - MBIO VL - 13 PY - 2022 IS - 1 PG - 21 SN - 2161-2129 DO - 10.1128/mBio.03144-21 UR - https://m2.mtmt.hu/api/publication/32607262 ID - 32607262 N1 - Funding Agency and Grant Number: New National Excellence Program of the Ministry for Innovation and Technology from the National Research, Development, and Innovation Fund [20391-3/2018/FEKUSTRAT, NKFIH K 123952, LP2018-15/2018, uNKP-20-5-SZTE-655]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00878/19/8]; National Research, Development, and Innovation Office-NKFIHNational Research, Development & Innovation Office (NRDIO) - Hungary; EU's Horizon 2020 research and innovation program [GINOP-2.3.2-15-2016-00038, GINOP-2.3.2-15-2016-00035]; Wellcome TrustWellcome TrustEuropean Commission [739593]; National Research, Development and Innovation Office-NKFIHNational Research, Development & Innovation Office (NRDIO) - Hungary [214229_Z_18_Z, PD 138450] Funding text: This work was supported by grant no. 20391-3/2018/FEKUSTRAT, NKFIH K 123952, LP2018-15/2018, and uNKP-20-5-SZTE-655 (M.K.) from the New National Excellence Program of the Ministry for Innovation and Technology from the National Research, Development, and Innovation Fund; by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (grant no. BO/00878/19/8 to M.K.) ; and by the National Research, Development, and Innovation Office-NKFIH through project no. GINOP-2.3.2-15-2016-00038 and GINOP-2.3.2-15-2016-00035. The project also received funding from the EU's Horizon 2020 research and innovation program under grant agreement no. 739593. J.R.N. was supported by the Wellcome Trust (grant no. 214229_Z_18_Z) . R.T. was financed by the National Research, Development and Innovation Office-NKFIH through grant no. PD 138450. AB - Oral squamous cell carcinoma (OSCC) is associated with oral Candida albicans infection, although it is unclear whether the fungus promotes the genesis and progression of OSCC or whether cancer facilitates fungal growth. In this study, we investigated whether C. albicans can potentiate OSCC tumor development and progression. In vitro, the presence of live C. albicans, but not Candida parapsilosis, enhanced the progression of OSCC by stimulating the production of matrix metalloproteinases, oncometabolites, protumor signaling pathways, and overexpression of prognostic marker genes associated with metastatic events. C. albicans also upregulated oncogenes in nonmalignant cells. Using a newly established xenograft in vivo mouse model to investigate OSCC-C. albicans interactions, oral candidiasis enhanced the progression of OSCC through inflammation and induced the overexpression of metastatic genes and significant changes in markers of the epithelial-mesenchymal transition. Finally, using the 4-nitroquinoline 1-oxide (4NQO) murine model, we directly correlate these in vitro and short-term in vivo findings with the progression of oncogenesis over the long term. Taken together, these data indicate that C. albicans upregulates oncogenes, potentiates a premalignant phenotype, and is involved in early and late stages of malignant promotion and progression of oral cancer. IMPORTANCE Oral squamous cell carcinoma (OSCC) is a serious health issue worldwide that accounts for 2% to 4% of all cancer cases. Previous studies have revealed a higher yeast carriage and diversity in oral cancer patients than in healthy individuals. Furthermore, fungal colonization in the oral cavity bearing OSCC is higher on the neoplastic epithelial surface than on adjacent healthy surfaces, indicating a positive association between oral yeast carriage and epithelial carcinoma. In addition to this, there is strong evidence supporting the idea that Candida contributes to carcinogenesis events in the oral cavity. Here, we show that an increase in Candida albicans burden promotes an oncogenic phenotype in the oral cavity. Copyright © 2022 Vadovics et al. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Dávid AU - Igaz, Nóra AU - Gopisetty, Mohana Krishna AU - Csontné Kiricsi, Mónika TI - Cancer Therapy by Silver Nanoparticles: Fiction or Reality? JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 2 PG - 26 SN - 1661-6596 DO - 10.3390/ijms23020839 UR - https://m2.mtmt.hu/api/publication/32592254 ID - 32592254 N1 - Department of Biochemistry and Molecular Biology, University of Szeged, Közép Fasor 52, Szeged, H-6726, Hungary CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur, 660 Route des Lucioles, Valbonne, 06560, France Interdisciplinary Center of Excellence, Department of Applied and Environmental Chemistry, University of Szeged, Rerrich Béla Tér 1, Szeged, H-6720, Hungary Cited By :9 Export Date: 16 November 2022 Correspondence Address: Kiricsi, M.; Department of Biochemistry and Molecular Biology, Közép Fasor 52, Hungary; email: kiricsim@gmail.com AB - As an emerging new class, metal nanoparticles and especially silver nanoparticles hold great potential in the field of cancer biology. Due to cancer-specific targeting, the consequently attenuated side-effects and the massive anti-cancer features render nanoparticle therapeutics desirable platforms for clinically relevant drug development. In this review, we highlight those characteristics of silver nanoparticle-based therapeutic concepts that are unique, exploitable, and achievable, as well as those that represent the critical hurdle in their advancement to clinical utilization. The collection of findings presented here will describe the features that distinguish silver nanoparticles from other anti-cancer agents and display the realistic opportunities and implications in oncotherapeutic innovations to find out whether cancer therapy by silver nanoparticles is fiction or reality. LA - English DB - MTMT ER - TY - CHAP AU - Nógrádi-Halmi, Dóra AU - Molnár-Gáspár, Renáta AU - Diószegi, Petra AU - Csontné Kiricsi, Mónika AU - Igaz, Nóra AU - Roland, Patai AU - Tamás, Polgár AU - László, Juhász AU - Csont, Tamás Bálint ED - László, Buday ED - Miklós, Erdélyi ED - Beáta, Lontay ED - József, Mihály ED - Sinka, Rita ED - László, Virág ED - Gergely, Szakáts ED - Attila, Varga TI - The role of mitoprotection in the cytoprotective effect of kynurenic acid against simulated ischemia/reoxygenation-induced cardiac cell damage T2 - Hungarian Molecular Life Sciences 2021 PB - Diamond Congress Kft. CY - Eger SN - 9786155270673 PY - 2021 SP - 140 UR - https://m2.mtmt.hu/api/publication/33104916 ID - 33104916 LA - English DB - MTMT ER -