TY - JOUR AU - Radványi, Lilla AU - Balázs, Viktória Lilla AU - Kocsis, Béla AU - Csikós, Eszter AU - Ángyán, Virág Diána AU - Szabó, Péter AU - Biró, Viktória AU - Kocsis, Marianna AU - Farkas, Ágnes TI - Antibacterial activity of Hungarian varietal honeys against respiratory pathogens as a function of storage time JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-024-60961-3 UR - https://m2.mtmt.hu/api/publication/34836449 ID - 34836449 N1 - Export Date: 15 May 2024 AB - Today, antibiotic therapies that previously worked well against certain bacteria due to their natural sensitivity, are becoming less effective. Honey has been proven to inhibit the biofilm formation of some respiratory bacteria, however few data are available on how the storage time affects the antibacterial effect. The activity of black locust, goldenrod, linden and sunflower honeys from three consecutive years (2020, 2021, 2022) was analyzed in 2022 against Gram-negative (Haemophilus influenzae, H. parainfluenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) bacteria using in vitro microbiological methods. After determining the physicochemical parameters of honey, broth microdilution was applied to determine the minimum inhibitory concentration of each honey type against each bacterium, and crystal violet assay was used to test their antibiofilm effect. The possible mechanism of action was explored with membrane degradation test, while structural changes were illustrated with scanning electron microscopy. Honeys stored for one or two years were darker than fresh honeys, while older honeys had significantly lower antibacterial activity. The most remarkable inhibitory effect was exerted by linden and sunflower honeys, and P. aeruginosa proved to be the most resistant bacterium. Based on our results, honey intended for medicinal purposes should be used as fresh as possible during a treatment. LA - English DB - MTMT ER - TY - CONF AU - Koloh, Regina AU - Farkas, Ágnes AU - Balázs, Viktória Lilla AU - Kocsis, Béla ED - Kovács, Bettina ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám TI - A gesztenyeméz biofilm képződést gátló hatása T2 - Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat C1 - Pécs SN - 9789636262457 PY - 2024 SP - 87 EP - 87 PG - 1 UR - https://m2.mtmt.hu/api/publication/34814487 ID - 34814487 LA - Hungarian DB - MTMT ER - TY - CONF AU - Nagy, Anett AU - Ormai, Edit AU - Horváth, Györgyi AU - Kocsis, Béla AU - Balázs, Viktória Lilla ED - Kovács, Bettina ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám TI - Mi az evidenciaalapú megközelítés alapján megítélt összefüggés a Pseudomonas aeruginosa baktérium és a kakukkfű illóolaj között? T2 - Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat C1 - Pécs SN - 9789636262457 PY - 2024 SP - 86 EP - 86 PG - 1 UR - https://m2.mtmt.hu/api/publication/34814458 ID - 34814458 LA - Hungarian DB - MTMT ER - TY - CONF AU - Ormai, Edit AU - Kocsis, Béla AU - Böszörményi, Andrea AU - Horváth, Györgyi AU - Balázs, Viktória Lilla ED - Kovács, Bettina ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám TI - Nozokomiális patogének és a szegfűszeg illóolaj kapcsolatának bemutatása evidenciaalapú megközelítésben T2 - Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat C1 - Pécs SN - 9789636262457 PY - 2024 SP - 83 EP - 83 PG - 1 UR - https://m2.mtmt.hu/api/publication/34814410 ID - 34814410 LA - Hungarian DB - MTMT ER - TY - CONF AU - Nagy, Anett AU - Ormai, Edit AU - Horváth, Györgyi AU - Kocsis, Béla AU - Balázs, Viktória Lilla ED - Szabó, Szilvia TI - Négy gyógyszerkönyvi illóolaj antibakteriális hatásának vizsgálata a Pseudomonas aeruginosa baktériummal szemben T2 - Abstract Book of the 7th International Cholnoky Symposium PY - 2024 SP - 23 EP - 23 PG - 1 UR - https://m2.mtmt.hu/api/publication/34795108 ID - 34795108 LA - Hungarian DB - MTMT ER - TY - CONF AU - Koloh, Regina AU - Farkas, Ágnes AU - Kocsis, Béla AU - Kocsis, Marianna AU - Balázs, Viktória Lilla ED - Szabó, Szilvia TI - A gesztenyeméz biofilmgátló hatása az idő függvényében T2 - Abstract Book of the 7th International Cholnoky Symposium PY - 2024 SP - 21 EP - 21 PG - 1 UR - https://m2.mtmt.hu/api/publication/34795054 ID - 34795054 LA - Hungarian DB - MTMT ER - TY - CONF AU - Ormai, Edit AU - Kocsis, Béla AU - Böszörményi, Andrea AU - Horváth, Györgyi AU - Balázs, Viktória Lilla ED - Szabó, Szilvia TI - A borsmenta illóolaj hatása kórházi környezetben előforduló patogéneken T2 - Abstract Book of the 7th International Cholnoky Symposium PY - 2024 SP - 19 EP - 19 PG - 1 UR - https://m2.mtmt.hu/api/publication/34795038 ID - 34795038 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Balázs, Viktória Lilla AU - Böszörményi, Andrea AU - Kocsis, Béla AU - Horváth, Györgyi TI - Gram-negative rough mutants used as test bacteria can increase sensitivity of direct bioautography JF - JPC - JOURNAL OF PLANAR CHROMATOGRAPHY - MODERN TLC J2 - J PLANAR CHROMATOGR MOD TLC PY - 2024 SN - 0933-4173 DO - 10.1007/s00764-024-00293-0 UR - https://m2.mtmt.hu/api/publication/34753258 ID - 34753258 N1 - Export Date: 16 May 2024 CODEN: JPCTE AB - Currently, the antimicrobial activity of essential oils (EOs) is an outstanding research field due to antibiotic resistance of microorganisms. Thin-layer chromatography‒direct bioautography (TLC‒DB) is an effective, fast method to find components with antimicrobial activity in a mixture of plant compounds, e.g., in EOs. The volatility and hydrophobic characters of EOs require special experimental conditions, and disc diffusion assay is not appropriate to explore the antimicrobial activity of them. The aim of this study was to use “R” mutants, which are more sensitive to synthetic antimicrobial drugs, in DB to increase the sensitivity of this method. Our hypothesis was that these mutants show sensitivity to some EOs (thyme, clove, and peppermint) as well. The chemical composition of our tested EOs was measured with gas chromatography‒mass spectrometry (GC‒MS). The main compounds (39.8% thymol, 78.8% eugenol, and 50.4% menthol) of EOs showed notable antibacterial activity in TLC‒DB. Based on our results, we suggest to use Salmonella minnesota Re595 rough strain as test bacterium in bioautography, because it showed the highest sensitivity to the tested antibiotics (gentamicin and cephalexin) and EOs. Furthermore, this rough mutant could make TLC‒DB more faster, because only 4 h incubation time was enough to detect the inhibition zones of the active compounds used in this study. LA - English DB - MTMT ER - TY - JOUR AU - Koloh, Regina AU - Balázs, Viktória Lilla AU - Radványi, Lilla AU - Kocsis, Béla AU - Kerekes, Erika Beáta AU - Kocsis, Marianna AU - Farkas, Ágnes TI - Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 13 PY - 2024 IS - 3 PG - 11 SN - 2079-6382 DO - 10.3390/antibiotics13030255 UR - https://m2.mtmt.hu/api/publication/34738553 ID - 34738553 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Office NKFIH K [132044]; New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development, and Innovation Fund [UNKP-23-3-I] Funding text: This research was funded by the grant from the National Research, Development, and Innovation Office NKFIH K 132044, and by the UNKP-23-3-I New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development, and Innovation Fund. AB - The irresponsible overuse of antibiotics has increased the occurrence of resistant bacterial strains, which represents one of the biggest patient safety risks today. Due to antibiotic resistance and biofilm formation in bacteria, it is becoming increasingly difficult to suppress the bacterial strains responsible for various chronic infections. Honey was proven to inhibit bacterial growth and biofilm development, offering an alternative solution in the treatment of resistant infections and chronic wounds. Our studies included chestnut honey, valued for its high antibacterial activity, and the bacteria Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and S. epidermidis, known to form multi-species biofilm communities. Minimum inhibitory concentrations (MIC) of chestnut honey were determined for each bacterial strain. Afterwards, the mixed bacterial biofilms were treated with chestnut honey at different stages of maturity (incubation times: 2, 4, 6, 12, 24 h). The extent of biofilm inhibition was measured with a crystal violet assay and demonstrated by scanning electron microscopy (SEM). As the incubation time increased and the biofilm became more mature, inhibition rates decreased gradually. The most sensitive biofilm was the combination MRSA-S. epidermidis, with a 93.5% inhibition rate after 2 h of incubation. Our results revealed that chestnut honey is suitable for suppressing the initial and moderately mature stages of mixed biofilms. LA - English DB - MTMT ER - TY - CONF AU - Ormai, Edit AU - Kocsis, Béla AU - Böszörményi, Andrea AU - Horváth, Györgyi AU - Balázs, Viktória Lilla ED - Ormai, Edit ED - Balázs, Viktória Lilla ED - Ollmann, Tamás TI - Nozokomiális infekciók és illóolajok kapcsolatának bemutatása evidenciaalapú megközelítésben T2 - Előadás-kivonatok: XXII. Grastyán Konferencia Tanuljunk egymástól! PY - 2023 SP - 10 EP - 10 PG - 1 UR - https://m2.mtmt.hu/api/publication/34491721 ID - 34491721 N1 - ISBN és DOI azonosító nélkül megjelent egyéb konferenciakötet, két külön pdf, de ugyanazokat az absztraktokat tartalmazza, az oldalszámok megegyeznek LA - English DB - MTMT ER -