TY - JOUR AU - Szerencsés, Bettina AU - Igaz, Nóra AU - Tóbiás, Ákos AU - Prucsi, Zsombor AU - Rónavári, Andrea AU - Bélteky, Péter AU - Madarász, Dániel AU - Papp, Csaba Gergő AU - Makra, Ildikó AU - Vágvölgyi, Csaba AU - Kónya, Zoltán AU - Pfeiffer, Ilona AU - Csontné Kiricsi, Mónika TI - Size-dependent activity of silver nanoparticles on the morphological switch and biofilm formation of opportunistic pathogenic yeasts JF - BMC MICROBIOLOGY J2 - BMC MICROBIOL VL - 20 PY - 2020 IS - 1 PG - 13 SN - 1471-2180 DO - 10.1186/s12866-020-01858-9 UR - https://m2.mtmt.hu/api/publication/31353819 ID - 31353819 N1 - Department of Microbiology, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Department of Applied and Environmental Chemistry, University of Szeged, Szeged, Hungary HAS-USZ Reaction Kinetics and Surface Chemistry Research Group, Szeged, Hungary Cited By :2 Export Date: 31 May 2021 Correspondence Address: Kiricsi, M.; Department of Biochemistry and Molecular Biology, Közép fasor 52, Hungary; email: kiricsim@gmail.com LA - English DB - MTMT ER - TY - JOUR AU - Fajka-Boja, Roberta AU - Suhajdáné Urbán, Veronika AU - Szebeni, Gábor AU - Czibula, Ágnes AU - Blaskó, Andrea AU - Kriston-Pál, Éva AU - Makra, Ildikó AU - Hornung, Ákos AU - Szabó, Enikő AU - Uher, Ferenc AU - Than, Nándor Gábor AU - Monostori, Éva TI - Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells JF - CYTOTHERAPY J2 - CYTOTHERAPY VL - 18 PY - 2016 IS - 3 SP - 360 EP - 370 PG - 11 SN - 1465-3249 DO - 10.1016/j.jcyt.2015.12.004 UR - https://m2.mtmt.hu/api/publication/3024122 ID - 3024122 AB - BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) have powerful immunosuppressive activity. This function of MSCs is attributed to plethora of the expressed immunosuppressive factors, such as galectin-1 (Gal-1), a pleiotropic lectin with robust anti-inflammatory effect. Nevertheless, whether Gal-1 renders or contributes to the immunosuppressive effect of MSCs has not been clearly established. Therefore, this question was the focus of a complex study. METHODS: MSCs were isolated from bone marrows of wild-type and Gal-1 knockout mice and their in vitro anti-proliferative and apoptosis-inducing effects on activated T cells were examined. The in vivo immunosuppressive activity was tested in murine models of type I diabetes and delayed-type hypersensitivity. RESULTS: Both Gal-1-expressing and -deficient MSCs inhibited T-cell proliferation. Inhibition of T-cell proliferation by MSCs was mediated by nitric oxide but not PD-L1 or Gal-1. In contrast, MSC-derived Gal-1 triggered apoptosis in activated T cells that were directly coupled to MSCs, representing a low proportion of the T-cell population. Furthermore, absence of Gal-1 in MSCs did not affect their in vivo immunosuppressive effect. CONCLUSIONS: These results serve as evidence that Gal-1 does not play a role in the systemic immunosuppressive effect of MSCs. However, a local contribution of Gal-1 to modulation of T-cell response by direct cell-to-cell interaction cannot be excluded. Notably, this study serves a good model to understand how the specificity of a pleiotropic protein depends on the type and localization of the producing effector cell and its target. LA - English DB - MTMT ER - TY - JOUR AU - Hegedüs, Zsófia AU - Makra, Ildikó AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Mándity, István AU - Monostori, Éva AU - Martinek, Tamás TI - Foldameric probes for membrane interactions by induced β-sheet folding JF - CHEMICAL COMMUNICATIONS J2 - CHEM COMMUN VL - 52 PY - 2016 IS - 9 SP - 1891 EP - 1894 PG - 4 SN - 1359-7345 DO - 10.1039/C5CC09257D UR - https://m2.mtmt.hu/api/publication/2993079 ID - 2993079 N1 - Funding Agency and Grant Number: Hungarian Academy of Sciences; Lendulet program [LP-2011-009]; [TAMOP-4.2.6-14/1]\n Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet program (LP-2011-009) and TAMOP-4.2.6-14/1. Csaba Vizler is gratefully acknowledged for providing the bEND.3 cell line.\n Funding Agency and Grant Number: Hungarian Academy of Sciences; Lendulet program [LP-2011-009]; [TAMOP-4.2.6-14/1] Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet program (LP-2011-009) and TAMOP-4.2.6-14/1. Csaba Vizler is gratefully acknowledged for providing the bEND.3 cell line. Funding Agency and Grant Number: Hungarian Academy of SciencesHungarian Academy of Sciences; Lendulet program [LP-2011-009]; [TAMOP-4.2.6-14/1] Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet program (LP-2011-009) and TAMOP-4.2.6-14/1. Csaba Vizler is gratefully acknowledged for providing the bEND.3 cell line. AB - Design strategies were devised for alpha/beta-peptide foldameric analogues of the antiangiogenic anginex with the goal of mimicking the diverse structural features from the unordered conformation to a folded beta-sheet in response to membrane interactions. Structure-activity relationships were investigated in the light of different beta-sheet folding levels. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Enikő AU - Fajka-Boja, Roberta AU - Kriston-Pál, Éva AU - Hornung, Ákos AU - Makra, Ildikó AU - Kudlik, Gyöngyi AU - Uher, Ferenc AU - Katona, Róbert László AU - Monostori, Éva AU - Czibula, Ágnes TI - Licensing by Inflammatory Cytokines Abolishes Heterogeneity of Immunosuppressive Function of Mesenchymal Stem Cell Population JF - STEM CELLS AND DEVELOPMENT J2 - STEM CELLS DEV VL - 24 PY - 2015 IS - 18 SP - 2171 EP - 2180 PG - 10 SN - 1547-3287 DO - 10.1089/scd.2014.0581 UR - https://m2.mtmt.hu/api/publication/2946565 ID - 2946565 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, 62 Temesvári krt, Szeged, 6726, Hungary Stem Cell Biology Unit, National Blood Service, Budapest, Hungary Cited By :38 Export Date: 10 February 2021 CODEN: SCDTA Correspondence Address: Czibula, A.; Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, 62 Temesvári krt, Hungary AB - When mesenchymal stem cells (MSCs) are used for therapy of immunological pathologies, they get into an inflammatory environment, altering the effectiveness of the treatment. To establish the impact of environmental inflammatory factors on MSCs' immunofunction in the mirror of intrinsic heterogeneity of mouse MSC population, individual MSC clones were generated and characterized. Adipogenic but not osteogenic differentiation and pro-angiogenic activity of five independent MSC cell lines were similar. Regarding osteogenic differentiation, clones MSC3 and MSC6 exhibited poorer capacity than MSC2, MSC4, and MSC5. To study the immunosuppressive heterogeneity, in vitro and in vivo experiments have been carried out using T-cell proliferation assay and delayed-type hypersensitivity (DTH) response, respectively. A remarkable difference was found between the clones in their ability to inhibit T-cell proliferation in the following order: MSC2MSC5>MSC4>MSC3>>MSC6. Nevertheless, the differences between the immunosuppressive activities of the individual clones disappeared on pretreatment of the cells with pro-inflammatory cytokines, a procedure called licensing. Stimulation of all clones with IFN- and TNF- resulted in elevation of their inhibitory capability to a similar level. Nitric oxide (NO) and prostaglandin E2 (PGE2) were identified as major mediators of immunofunction of the MSC clones. The earlier findings were also supported by in vivo results. Without licensing, MSC2 inhibited DTH response, while MSC6 did not affect DTH response. In contrast, prestimulation of MSC6 with inflammatory cytokines resulted in strong suppression by this clone as well. Here, we have showed that MSC population is functionally heterogeneous in terms of immunosuppressive function; however, this variability is largely reduced under pro-inflammatory conditions. LA - English DB - MTMT ER - TY - JOUR AU - Hegedüs, Zsófia AU - Wéber, Edit AU - Kriston-Pál, Éva AU - Makra, Ildikó AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Foldameric α/β-Peptide Analogs of the β-Sheet-Forming Antiangiogenic Anginex: Structure and Bioactivity JF - JOURNAL OF THE AMERICAN CHEMICAL SOCIETY J2 - J AM CHEM SOC VL - 135 PY - 2013 IS - 44 SP - 16578 EP - 16584 PG - 7 SN - 0002-7863 DO - 10.1021/ja408054f UR - https://m2.mtmt.hu/api/publication/2459240 ID - 2459240 AB - The principles of beta-sheet folding and design for alpha-peptidic sequences are well established, while those for sheet mimetics containing homologated amino acid building blocks are still under investigation. To reveal the structure-function relations of beta-amino-acid-containing foldamers, we followed a top-down approach to study a series of alpha/beta-peptidic analogs of anginex, a beta-sheet-forming antiangiogenic peptide. Eight anginex analogs were developed by systematic alpha --> beta(3) substitutions and analyzed by using NMR and CD spectroscopy. The foldamers retained the beta-sheet tendency, though with a decreased folding propensity. beta-Sheet formation could be induced by a micellar environment, similarly to that of the parent peptide. The destructuring effect was higher when the alpha --> beta(3) exchange was located in the beta-sheet core. Analysis of the beta-sheet stability versus substitution pattern and the local conformational bias of the bulky beta(3)V and beta(3)I residues revealed that a mismatch between the H-bonding preferences of the alpha- and beta-residues played a minor role in the structure-breaking effect. Temperature-dependent CD and NMR measurements showed that the hydrophobic stabilization was scaled-down for the alpha/beta-peptides. Analysis of the biological activity of the foldamer peptides showed that four anginex derivatives dose-dependently inhibited the proliferation of a mouse endothelial cell line. The alpha --> beta(3) substitution strategy applied in this work can be a useful approach to the construction of bioactive beta-sheet mimetics with a reduced aggregation tendency and improved pharmacokinetic properties. LA - English DB - MTMT ER -