TY - JOUR AU - Al Kateeb, Anfal Izaldeen Mutar AU - Tüfekci, Enis Fuat AU - Altunoglu, Yasemin Celik AU - Baloglu, Mehmet Cengiz AU - Nilofar, Nilufar AU - Yıldıztugay, Evren AU - Jekő, József AU - Cziáky, Zoltán AU - Zengin, Gokhan TI - Multidirectional research for the therapeutic potential of Phlomoides molucelloides (Bunge) Salmaki: LC-MS/MS, antioxidant, enzyme inhibition, and antiproliferative characteristics JF - PROCESS BIOCHEMISTRY J2 - PROCESS BIOCHEM VL - 143 PY - 2024 SP - 302 EP - 314 PG - 13 SN - 1359-5113 DO - 10.1016/j.procbio.2024.05.011 UR - https://m2.mtmt.hu/api/publication/34864806 ID - 34864806 LA - English DB - MTMT ER - TY - JOUR AU - Zengin, Gokhan AU - Terzić, Milena AU - Abul, Nurgul AU - Gulcin, Ilhami AU - Koyuncu, Ismail AU - Basarali, Mustafa Kemal AU - Đorđević, Tatjana AU - Cziáky, Zoltán AU - Jekő, József AU - Cespedes- Acuna, Carlos L. TI - A multidimensional study for design functional foods: Chemical profiling, antioxidant potential, enzyme inhibition, and cytotoxic effects of Alkanna tubulosa extracts JF - FOOD BIOSCIENCE J2 - FOOD BIOSCI VL - 60 PY - 2024 SP - 104280 SN - 2212-4292 DO - 10.1016/j.fbio.2024.104280 UR - https://m2.mtmt.hu/api/publication/34844748 ID - 34844748 N1 - Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University Campus, Konya, 42130, Turkey Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia Department of Chemistry, Faculty of Sciences, Ataturk University 25240-Erzurum, Turkey Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, 63290, Turkey Department of Medical Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, Turkey Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, Hungary Plant Biochemistry and Phytochemical Ecology Lab, Departmento de Ciencias Basicas, Facultad de Ciencias, Universidad del Bio Bio, Av. Andres Bello #720, Chillan, Chile Export Date: 15 May 2024; Cited By: 0; Correspondence Address: M. Terzić; Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia; email: milenavujanovic@uns.ac.rs LA - English DB - MTMT ER - TY - JOUR AU - Szűcs, Zsolt AU - Cziáky, Zoltán AU - Volánszki, László AU - Máthé, Csaba AU - Vasas, Gábor AU - Gonda, Sándor TI - Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 13 PY - 2024 IS - 10 PG - 19 SN - 2223-7747 DO - 10.3390/plants13101288 UR - https://m2.mtmt.hu/api/publication/34843399 ID - 34843399 AB - Medicinal plant tissue cultures are potential sources of bioactive compounds. In this study, we report the chemical characterization of the callus cultures of three medicinal Tilia spp. (Tilia cordata, Tilia vulgaris and Tilia tomentosa), along with the comparison to bracts and flowers of the same species. Our aim was to show that calli of Tilia spp. are good alternatives to the calli of T. americana for the production of polyphenols and are better sources of a subset of polyphenolic metabolites, compared to the original organs. Calli were initiated from young bracts and grown on woody plant medium containing 1 mg L−1 2,4-D and 0.1 mg L−1 BAP. For chemical characterization, a quality-controlled untargeted metabolomics approach and the quantification of several bioactive compounds was performed with the use of LC-ESI-MS/MS. While bracts and flowers contained flavonoid glycosides (astragalin, isoquercitrin) as major polyphenols, calli of all species contained catechins, coumarins (fraxin, esculin and scopoletin) and flavane aglyca. T. tomentosa calli contained 5397 µg g DW−1 catechin, 201 µg g DW−1 esculin, 218 µg g DW−1 taxifolin and 273 µg g DW−1 eriodictyol, while calli from other species contained lower amounts. T. cordata and T. tomentosa flowers were rich in isoquercitrin, containing 8134 and 6385 µg g DW−1, respectively. The currently tested species contained many of the bioactive metabolites described from T. americana. The production of catechin was shown to be comparable to the most efficient tissue cultures reported. Flowers and bracts contained flavonoid glycosides, including tiliroside, resembling bioactive fractions of T. americana. In addition, untargeted metabolomics has shown fingerprint-like differences among species, highlighting possible chemotaxonomic and quality control applications, especially for bracts. LA - English DB - MTMT ER - TY - JOUR AU - Zengin, Gokhan AU - Yagi, Sakina AU - Ibrahime, Sinan AU - Yildiztugay, Evren AU - Jekő, József AU - Cziáky, Zoltán AU - Aktumsek, Abdurrahman TI - Screening for chemical components, antioxidant and anti-enzymatic abilites activities of three Astragalus species from Turkey JF - JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS J2 - J BIOL REG HOMEOS AG VL - 38 PY - 2024 IS - 2 SP - 973 EP - 987 PG - 15 SN - 0393-974X DO - 10.23812/j.biol.regul.homeost.agents.20243802.78 UR - https://m2.mtmt.hu/api/publication/34551425 ID - 34551425 AB - Background: In Turkey, Astragalus species are highly appreciated for their traditional therapeutic values. This research was devised to investigate the chemical composition, antioxidant, and enzyme inhibition activities of the aerial components of Astragalus (A.) angustifolius subsp. pungens, A. prusianus, and A. zederbaueri. Methods: Four extracts namely; ethyl acetate (EtOAc), methanol (MeOH), 70% MeOH, and aqueous extracts, were meticulously prepared. The chemical composition was examined utilizing spectrophotometric and High performance liquid chromatography-mass spectrometry (HPLC-MS)/MS techniques. The antioxidant activity was assessed by investigating the extracts' capability to scavenge radicals, reduce ions, and chelate ferrous ions. The enzyme inhibition properties were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase (Tyr), α-glucosidase, and α-amylase enzymes. Results: The findings revealed that the greatest total phenolic (28.33 mg gallic acid equivalent/g) and flavonoid (66.59 mg rutin equivalent/g) contents were achieved with the MeOH extract of A. angustifolius. The chemical profiles of the three species were marked by the presence of flavonol glycosides containing kaempferol, rhamnetin, isorhamnetin, and quercetin structural motifs. MeOH extract of A. angustifolius exerted the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (48.73 mg trolox equivalent (TE)/g), Cu++ (150.10 mg TE/g) and Fe+++ (84.78 mg TE/g) reducing capacity. The three polar extracts (67.18–69.64 mg TE/g) of this species in addition to the aqueous extract (69.46 mg TE/g) of A. zederbaueri displayed the best 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. 70% MeOH extract of A. prusianus had the highest metal chelating power (25.61 mg ethylenediaminetetraacetate equivalent (EDTAE)/g). MeOH extracts (2.37–2.50 mg galatamine equivalent (GALAE)/g) of the three species revealed the highest AChE inhibition activity while EtOAc extract (2.01 mg GALAE/g) of A. zederbaueri had the highest BChE inhibition activity. The highest anti-Tyr (50.40 mg kojic acid equivalent/g) activity was recorded from the EtOAc extract of A. zederbaueri. Conclusion: Thus, the three species of the studied Astragalus may represent a promising natural source of pyhtopharmaceutical agents for various pharmaceutical and nutritional uses. LA - English DB - MTMT ER - TY - JOUR AU - Custódio, L. AU - Cziáky, Zoltán AU - Castañeda-Loaiza, V. AU - Rodrigues, M.J. TI - Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties JF - PLANT CELL TISSUE AND ORGAN CULTURE J2 - PLANT CELL TISS ORG VL - 156 PY - 2024 IS - 2 SN - 0167-6857 DO - 10.1007/s11240-024-02690-z UR - https://m2.mtmt.hu/api/publication/34543379 ID - 34543379 N1 - Centre of Marine Sciences, (CCMAR/CIMAR LA), University of Algarve, Campus of Gambelas, Faro, 8005-139, Portugal Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, 4400, Hungary Export Date: 30 January 2024; Cited By: 0; Correspondence Address: M.J. Rodrigues; Centre of Marine Sciences, (CCMAR/CIMAR LA), University of Algarve, Faro, Campus of Gambelas, 8005-139, Portugal; email: mjrodrigues@ualg.pt; CODEN: PTCED LA - English DB - MTMT ER - TY - JOUR AU - Tewari, Devesh AU - Bawari, Sweta AU - Mishra, Saurabh T AU - Gupta, Pawan AU - M, Aryalaxmi AU - Cziáky, Zoltán AU - Jekő, József AU - Lazarova, Irina AU - Zengin, Gökhan TI - Metabolomics‐based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.‐Ham. ex C.B. Clarke from the western Himalayas JF - JOURNAL OF SEPARATION SCIENCE J2 - J SEP SCI VL - 47 PY - 2024 IS - 1 SN - 1615-9306 DO - 10.1002/jssc.202300795 UR - https://m2.mtmt.hu/api/publication/34502575 ID - 34502575 N1 - Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Delhi Pharmaceutical Sciences and Research University, New Delhi, India Department of Pharmacology, Amity Institute of Pharmacy, Amity University, Noida, India Department of Career Services, Edvancer - a part of Global University System, India, Mumbai, India Department of Pharmaceutical Chemistry, Shri Vile Parle Kelavani Mandal's Institute of Pharmacy, Dhule, India Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyiregyhaza, Hungary Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, Sofia, Bulgaria Department of Biology, Faculty of Science, Selçuk University, Konya, Turkey Export Date: 25 January 2024 CODEN: JSSCC Correspondence Address: Tewari, D.; Department of Pharmacognosy and Phytochemistry, India; email: devesh.tewari@dpsru.edu.in Correspondence Address: Zengin, G.; Department of Biology, Turkey; email: gokhanzengin@selcuk.edu.tr AB - Swertia species are common ingredients in numerous herbal remedies. It is also used to treat a wide range of illnesses and possess diverse therapeutic activities. The aim of the study is to elucidate the comprehensive metabolomics profile of Swertia chirayita and the role of various extraction methods in the phytochemical compositions of the extracts of S. chirayita , and their antioxidant and enzyme inhibitory activities. Extraction of the stems, leaves, and flowering tops of S. chirayita was performed by maceration, infusion, and soxhlation using methanol and water as solvent. Extracts were subjected to phytochemical profiling by a liquid‐chromatographic system. Antioxidant and enzyme inhibitory activity was carried out. The metabolomics profiling showed that a diverse range of specialized metabolites were present in the stems and leaves & flowering tops of the plant. All the extracts showed substantial antioxidant and enzyme inhibitory activities further confirmed by molecular docking studies. This study appraised the use of S. chirayita aerial parts as a potential antioxidant and its therapeutic application in various chronic illnesses including Alzheimer's disease, diabetes, and other skin‐related disorders. LA - English DB - MTMT ER - TY - JOUR AU - Mirzaei, Hasan AU - Jekő, József AU - Cziáky, Zoltán AU - Jahanshahi, Mehrdad AU - Zengin, Gokhan AU - Enayati, Aysheh TI - LC–MS/MS phytochemical profiling, antioxidant activity, and enzyme inhibitory of Potentilla reptans L. root: Computational studies and experimental validation JF - PROCESS BIOCHEMISTRY J2 - PROCESS BIOCHEM VL - 137 PY - 2024 SP - 30 EP - 40 PG - 11 SN - 1359-5113 DO - 10.1016/j.procbio.2023.12.013 UR - https://m2.mtmt.hu/api/publication/34442108 ID - 34442108 N1 - Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, Hungary Neuroscience Research Center, Department of Anatomy, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey Export Date: 15 January 2024; Cited By: 0; Correspondence Address: G. Zengin; Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey; email: gokhanzengin@selcuk.edu.tr; CODEN: PBCHE LA - English DB - MTMT ER - TY - JOUR AU - Youssef, Seria AU - Custódio, Luisa AU - Rodrigues, Maria João AU - Pereira, Catarina G. AU - Calhelha, Ricardo C. AU - Jekő, József AU - Cziáky, Zoltán AU - Ben Hamed, Karim TI - Harnessing the Bioactive Potential of Limonium spathulatum (Desf.) Kuntze: Insights into Enzyme Inhibition and Phytochemical Profile JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 12 PY - 2023 IS - 19 SN - 2223-7747 DO - 10.3390/plants12193391 UR - https://m2.mtmt.hu/api/publication/34157617 ID - 34157617 N1 - Laboratory of Extremophile Plants, Center of Biotechnology of BorjCedria, Hammam-Lif, 2050, Tunisia Centre of Marine Sciences (CCMAR), Universidade do Algarve, Campus de Gambelas, Faro, 8005-139, Portugal Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança, 5300-253, Portugal Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, 4405, Hungary Export Date: 27 October 2023 Correspondence Address: Ben Hamed, K.; Laboratory of Extremophile Plants, Tunisia; email: karim.benhamed@cbbc.rnrt.tn AB - This study assessed the halophyte species Limonium spathulatum (Desf.) as a possible source of natural ingredients with the capacity to inhibit enzymes related to relevant human health disorders and food browning. Extracts using food-grade solvents such as water and ethanol were prepared by maceration from dried L. spathulatum leaves. They were evaluated for in vitro inhibition activity of enzymes such as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), α-glucosidase, tyrosinase and lipase, related to Alzheimer’s disease, type-2-diabetes mellitus, skin hyperpigmentation, and obesity, respectively. These extracts were also appraised for in vitro acute toxicity on tumoral and non-tumoral cell lines and their chemical composition by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). The extracts were more effective towards BChE than AChE. The best results were obtained with the hydroethanolic and water extracts, with IC50 values of 0.03 mg/mL and 0.06 mg/mL, respectively. The hydroethanolic extract had the highest capacity to inhibit α-glucosidase (IC50: 0.04 mg/mL), higher than the positive control used (acarbose, IC50 = 3.14 mg/mL). The ethanol extract displayed the best inhibitory activity against tyrosinase (IC50 = 0.34 mg/mL). The tested samples did not inhibit lipase and exhibited low to moderate cytotoxic activity against the tested cell lines. The hydroethanolic extract had a higher diversity of compounds, followed by the ethanol and water samples. Similar molecules were identified in all the extracts and were mainly hydroxybenzoic acids, hydroxycinnamic acids, and flavonoids. Taken together, these results suggest that L. spathulatum should be further explored as a source of bioactive ingredients for the food, cosmetic, and pharmaceutical industries. LA - English DB - MTMT ER - TY - JOUR AU - Alaya, Amina AU - Mihok, Emőke AU - Pecsenye, Bence AU - Jolji, Maria AU - Kertész, Attila AU - Bársony, Péter AU - Vigh, Szabolcs AU - Cziáky, Zoltán AU - Máthé , Anna AU - Burtescu, Ramona Flavia AU - Oláh, Neli-Kinga AU - Neamțu, Andreea-Adriana AU - Turcuș, Violeta AU - Máthé, Endre TI - Phytoconstituent Profiles Associated with Relevant Antioxidant Potential and Variable Nutritive Effects of the Olive, Sweet Almond, and Black Mulberry Gemmotherapy Extracts JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 12 PY - 2023 IS - 9 PG - 36 SN - 2076-3921 DO - 10.3390/antiox12091717 UR - https://m2.mtmt.hu/api/publication/34129813 ID - 34129813 N1 - Doctoral School of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi Str. 128, Debrecen, 4032, Hungary Doctoral School of Nutrition and Food Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi Str. 128, Debrecen, 4032, Hungary Institute of Nutrition Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi Str. 128, Debrecen, 4032, Hungary Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi Str. 128, Debrecen, 4032, Hungary Agricultural and Molecular Research Institute, University of Nyíregyháza, Sóstói Str. 31, Nyíregyháza, 4400, Hungary Doctoral School of Neuroscience, Faculty of Medicine, University of Debrecen, Nagyerdei Str. 94, Debrecen, 4032, Hungary PlantExtrakt Ltd, Cluj, 407059, Romania Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Vasile Goldiș Western University from Arad, L.Rebreanu Str. 86, Arad, 310414, Romania Department of Life Sciences, Faculty of Medicine, Vasile Goldiș Western University from Arad, L.Rebreanu Str. 86, Arad, 310414, Romania CE-MONT Mountain Economy Center, Costin C. Kirițescu National Institute of Economic Research, Romanian Academy, Petreni Str. 49, Suceava, 725700, Romania Export Date: 27 October 2023 Correspondence Address: Turcuș, V.; Department of Life Sciences, L.Rebreanu Str. 86, Romania; email: violeta_buruiana@yahoo.com AB - The extracts of whole plants or specific organs from different plant species are gaining increasing attention for their phytotherapy applications. Accordingly, we prepared standardized gemmotherapy extracts (GTEs) from young shoots/buds of olive (Olea europaea), sweet almond (Prunus amygdalus), and black mulberry (Morus nigra), and analyzed the corresponding phytonutrient profiles. We identified 42, 103, and 109 phytonutrients in the olive, almond, and black mulberry GTEs, respectively, containing amino acids, vitamins, polyphenols, flavonoids, coumarins, alkaloids, iridoids, carboxylic acids, lignans, terpenoids, and others. In order to assess the physiological effects generated by the GTEs, we developed a translational nutrition model based on Drosophila melanogaster and Cyprinus carpio. The results indicate that GTEs could influence, to a variable extent, viability and ATP synthesis, even though both are dependent on the specific carbohydrate load of the applied diet and the amino acid and polyphenol pools provided by the GTEs. It seems, therefore, likely that the complex chemical composition of the GTEs offers nutritional properties that cannot be separated from the health-promoting mechanisms that ultimately increase viability and survival. Such an approach sets the paves the way for the nutritional genomic descriptions regarding GTE-associated health-promoting effects. LA - English DB - MTMT ER - TY - JOUR AU - Zengin, Gokhan AU - Yagi, Sakina AU - Selvi, Selami AU - Cziáky, Zoltán AU - Jekő, József AU - Sinan, Kouadio Ibrahime AU - Topcu, Asena Ayyüce AU - Erci, Fatih AU - Boczkaj, Grzegorz TI - Elucidation of chemical compounds in different extracts of two Lavandula taxa and their biological potentials: Walking with versatile agents on the road from nature to functional applications JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 204 PY - 2023 PG - 11 SN - 0926-6690 DO - 10.1016/j.indcrop.2023.117366 UR - https://m2.mtmt.hu/api/publication/34112875 ID - 34112875 N1 - Department of Biology, Science Faculty, Selcuk University, Konya, 42130, Turkey Department of Botany, Faculty of Science, University of Khartoum, Sudan Department of Plant and Animal Production, Altınoluk Vocational School, Balıkesir University, Balıkesir, Turkey Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, 4400, Hungary Necmettin Erbakan University, Faculty of Science, Department of Biotechnology, Konya, Turkey Department of Sanitary Engineering, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, Poland Export Date: 14 September 2023 CODEN: ICRDE Correspondence Address: Zengin, G.; Department of Biology, Turkey; email: gokhanzengin@selcuk.edu.tr LA - English DB - MTMT ER -