TY - JOUR AU - Farkas, Enikő AU - Kovács, Kinga Dóra AU - Székács, Inna AU - Péter, Beatrix AU - Lagzi, István László AU - Kitahata, Hiroyuki AU - Suematsu, Nobuhiko J. AU - Horváth, Róbert TI - Kinetic monitoring of molecular interactions during surfactant-driven self-propelled droplet motion by high spatial resolution waveguide sensing JF - JOURNAL OF COLLOID AND INTERFACE SCIENCE J2 - J COLLOID INTERF SCI VL - 677 PY - 2025 SP - 352 EP - 364 PG - 13 SN - 0021-9797 DO - 10.1016/j.jcis.2024.07.236 UR - https://m2.mtmt.hu/api/publication/35173267 ID - 35173267 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office of Hungary [K131425, 146071]; National Research, Development, and Innovation Fund of Hungary [TKP2021-EGA-02, TKP2021-EGA-04]; JSPS Japan-Hungary Bilateral Joint Research Project [JPJSBP 120213801]; Hungarian Academy of Sciences; Ministry of Innovation and Technology from the source of the National Research, Development and Innovation Fund Funding text: The authors appreciate Prof. Satoshi Nakata for his helpful advice and discussion. This work was supported by the National Research, Development and Innovation Office of Hungary (K131425 and 146071) , the National Research, Development, and Innovation Fund of Hungary under Grant TKP2021-EGA-02, TKP2021-EGA-04, HUN-REN Hungarian Research Network, and JSPS Japan-Hungary Bilateral Joint Research Project (JPJSBP 120213801) . This project was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (for B.P.) . This work was supported by the KDP-2021 program of the Ministry of Innovation and Technology from the source of the National Research, Development and Innovation Fund. Part number: B AB - Hypothesis: Self-driven actions, like motion, are fundamental characteristics of life. Today, intense research focuses on the kinetics of droplet motion. Quantifying macroscopic motion and exploring the underlying mechanisms are crucial in self-structuring and self-healing materials, advancements in soft robotics, innovations in self-cleaning environmental processes, and progress within the pharmaceutical industry. Usually, the driving forces inducing macroscopic motion act at the molecular scale, making their real-time and high-resolution investigation challenging. Label-free surface sensitive measurements with high lateral resolution could in situ measure both molecular-scale interactions and microscopic motion. Experiments: We employ surface-sensitive label-free sensors to investigate the kinetic changes in a self-assembled monolayer of the trimethyl(octadecyl)azanium chloride surfactant on a substrate surface during the self-propelled motion of nitrobenzene droplets. The adsorption–desorption of the surfactant at various concentrations, its removal due to the moving organic droplet, and rebuilding mechanisms at droplet-visited areas are all investigated with excellent time, spatial, and surface mass density resolution. Findings: We discovered concentration dependent velocity fluctuations, estimated the adsorbed amount of surfactant molecules, and revealed multilayer coverage at high concentrations. The desorption rate of surfactant (18.4 s−1) during the microscopic motion of oil droplets was determined by in situ differentiating between droplet visited and non-visited areas. LA - English DB - MTMT ER - TY - GEN AU - Kanyó, Nicolett AU - Kovács, Kinga Dóra AU - Balogh, Anna AU - Rajmon, Imola AU - Péter, Beatrix AU - Székács, Inna AU - Lagzi, István AU - Molnár, Kinga AU - Truszka, Mónika AU - Misao, Fujiionika AU - Tomaso, Zambelli AU - Hideyuki, Nakanish AU - Horváth, Róbert TI - Funkcionalizált arany nanorészecskék sejten belüli viselkedése FluidFM-alapú nanoinjekcióval PY - 2025 UR - https://m2.mtmt.hu/api/publication/36159627 ID - 36159627 LA - Hungarian DB - MTMT ER - TY - GEN AU - Horváth, Róbert AU - Porkoláb, Gergő AU - Magyaródi, Beatrix AU - Rajmon, Imola AU - Kovács, Kinga Dóra AU - Kanyó, Nicolett AU - Varga, Dános Sebestyén AU - Walter, Fruzsina AU - Martins, Ana AU - Péter, Beatrix AU - Székács, Inna AU - Dér, András AU - Deli, Mária Anna TI - LABEL-FREE BIOPHYSICAL TOOLS FOR CELLULAR ADHESION, BIOMECHANICS, AND SINGLE-CELL INJECTION PY - 2025 UR - https://m2.mtmt.hu/api/publication/36161130 ID - 36161130 LA - English DB - MTMT ER - TY - JOUR AU - Kanyó, Nicolett AU - Borbély, Krisztina AU - Péter, Beatrix AU - Kovács, Kinga Dóra AU - Balogh, Anna AU - Magyaródi, Beatrix AU - Kurunczi, Sándor AU - Székács, Inna AU - Horváth, Róbert TI - Kinetic Analysis of SARS-CoV-2 S1-Integrin Binding Using Live-Cell, Label-Free Optical Biosensing JF - BIOSENSORS J2 - BIOSENSORS-BASEL VL - 15 PY - 2025 IS - 8 SN - 2079-6374 DO - 10.3390/bios15080534 UR - https://m2.mtmt.hu/api/publication/36293154 ID - 36293154 N1 - Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [TKP2021-EGA04]; HUN-REN Hungarian Research Network; National Research, Development and Innovation Office of Hungary [K146071]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; University Research Scholarship Program of the University of Pannonia [2024-2.1.1-EKOP-2024-00025]; EKOP-24 University Excellence Scholarship Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund [EKOP-24-3-I-ELTE-525] Funding text: This research was funded by the TKP2021-EGA04 (TKP2021 funding scheme) and KDP-2021 programs provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund. Additionally, this project was supported by the HUN-REN Hungarian Research Network, the National Research, Development and Innovation Office of Hungary (K146071), and the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (for B.P.). Further support was provided by the University Research Scholarship Program 2024-2.1.1-EKOP-2024-00025 of the University of Pannonia (for N.K.), as well as by the EKOP-24 University Excellence Scholarship Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund (EKOP-24-3-I-ELTE-525) (for A.B.). AB - The SARS-CoV-2 spike (S1) protein facilitates viral entry through binding to angiotensin-converting enzyme 2 (ACE2), but it also contains an Arg–Gly–Asp (RGD) motif that may enable interactions with RGD-binding integrins on ACE2-negative cells. Here, we provide quantitative evidence for this alternative binding pathway using a live-cell, label-free resonant waveguide grating (RWG) biosensor. RWG technology allowed us to monitor real-time adhesion kinetics of live cells to RGD-displaying substrates, as well as cell adhesion to S1-coated surfaces. To characterize the strength of the integrin–S1 interaction, we determined the dissociation constant using two complementary approaches. First, we performed a live-cell competitive binding assay on RGD-displaying surfaces, where varying concentrations of soluble S1 were added to cell suspensions. Second, we recorded the adhesion kinetics of cells on S1-coated surfaces and fitted the data using a kinetic model based on coupled ordinary differential equations. By comparing the results from both methods, we estimate that approximately 33% of the S1 molecules immobilized on the Nb2O5 biosensor surface are capable of initiating integrin-mediated adhesion. These findings support the existence of an alternative integrin-dependent entry route for SARS-CoV-2 and highlight the effectiveness of label-free RWG biosensing for quantitatively probing virus–host interactions under physiologically relevant conditions without the need of the isolation of the interaction partners from the cells. LA - English DB - MTMT ER - TY - GEN AU - Borbély, Krisztina AU - Balogh, Dóra Anett AU - Kurunczi, Sándor AU - Farkas, Enikő AU - Péter, Beatrix AU - Székács, Inna AU - Endre, Gabriella AU - Kondorosi, Eva AU - Horváth, Róbert TI - From protein-peptide interactions to cell adhesion: label-free biophysical insights into peptide activity PY - 2025 UR - https://m2.mtmt.hu/api/publication/36333981 ID - 36333981 LA - English DB - MTMT ER - TY - GEN AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Székács, Inna AU - Bősze, Szilvia AU - M. Kovács, Gábor AU - Boldizsár, Imre AU - Horváth, Róbert TI - Label-free biosensing of lignan compounds applying engineered model surfaces PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334122 ID - 36334122 LA - English DB - MTMT ER - TY - GEN AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Székács, Inna AU - Bősze, Szilvia AU - M. Kovács, Gábor AU - Boldizsár, Imre AU - Horváth, Róbert TI - Label-free biosensing of lignan compounds for therapeutics PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334130 ID - 36334130 LA - English DB - MTMT ER - TY - GEN AU - Farkas, Enikő AU - Kovács, Kinga Dóra AU - Székács, Inna AU - Péter, Beatrix AU - Lagzi, Istvan AU - Kitahata, Hiroyuki AU - J. Suematsu, Nobuhiko AU - Horváth, Róbert TI - Self-Propelled Droplet Motion with Characterization of Real-Time Kinetic Analysis of Surfactant Interactions by Label-Free Surface-Sensitive Biosensors PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334145 ID - 36334145 LA - English DB - MTMT ER - TY - GEN AU - Farkas, Enikő AU - Kovács, Kinga Dóra AU - Székács, Inna AU - Péter, Beatrix AU - Lagzi, Istvan AU - Kitahata, Hiroyuki AU - J. Suematsu, Nobuhiko AU - Horváth, Róbert TI - Real time, Nanometer Scale Resolution Kinetic Detection of Self Propelled Droplet Motion by Waveguide Biosensor PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334149 ID - 36334149 LA - English DB - MTMT ER - TY - GEN AU - Balogh, Dóra Anett AU - Novák, Szabolcs AU - Farkas, Enikő AU - Péter, Beatrix AU - Domokos, Heléna Brigitta AU - Szabó, Bálint AU - Horváth, Róbert TI - Technical advancements in circulating tumor cell sorting using a piezoelectric micropipette PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334158 ID - 36334158 LA - English DB - MTMT ER -