TY - JOUR AU - Laczkó-Dobos, Hajnalka AU - Bhattacharjee, Arindam AU - Maddali, Asha Kiran AU - Kincses, András AU - Abuammar, Hussein AU - Sebőkné Nagy, Krisztina AU - Páli, Tibor AU - Dér, András AU - Hegedűs, Tamás AU - Csordás, Gábor AU - Juhász, Gábor TI - PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion JF - AUTOPHAGY J2 - AUTOPHAGY VL - 0 PY - 2024 SP - 0 SN - 1554-8627 DO - 10.1080/15548627.2024.2322493 UR - https://m2.mtmt.hu/api/publication/34724664 ID - 34724664 N1 - Institute of Genetics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Doctoral School of Biology, University of Szeged, Szeged, Hungary Institute of Biophysics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary HUN-REN Biophysical Virology Research Group, Budapest, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Export Date: 27 March 2024 Correspondence Address: Juhász, G.; HUN-REN Biological Research Centre Szeged, Temesvari krt. 62, Hungary; email: juhasz.gabor@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Deli, Mária Anna AU - Porkoláb, Gergő AU - Kincses, András AU - Mészáros, Mária AU - Szecskó, Anikó AU - Kocsis, Anna AU - Vigh, Judit Piroska AU - Valkai, Sándor AU - Veszelka, Szilvia AU - Walter, Fruzsina AU - Dér, András TI - Lab-on-a-chip models of the blood-brain barrier: evolution, problems, perspectives JF - LAB ON A CHIP J2 - LAB CHIP PY - 2024 PG - 34 SN - 1473-0197 DO - 10.1039/d3lc00996c UR - https://m2.mtmt.hu/api/publication/34673907 ID - 34673907 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [K-143766, K-124922, FK-143233, PD-138930, PD-143268]; Hungarian Research Network [SA-111/2021]; Centenarian Foundation; Talentum Foundation of Gedeon Richter Plc. [UNKP-23-3-SZTE-517, UNKP-23-3-SZTE-535]; National Academy of Scientist Education Program of the National Biomedical Foundation under Hungarian Ministry of Culture and Innovation; New National Excellence Program of the Hungarian Ministry of Culture and Innovation; [H-1103]; [19-21]; [UNKP-23-3-SZTE-497] Funding text: The following funding was received from the National Research, Development and Innovation Office, Hungary: grants K-143766 (to M. A. D.), K-124922 (to A. D.), FK-143233 (to S. V.), PD-138930 (to M. M.), PD-143268 (to A. K.). F. R. W. was supported by the grant SA-111/2021 from the Hungarian Research Network. M. M. was supported by the Centenarian Foundation, A. S. and J. P. V. by the Talentum Foundation of Gedeon Richter Plc. (H-1103 Budapest, Gyoemr & odblac;i str. 19-21. Hungary). G. P. was supported by the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation. The New National Excellence Program of the Hungarian Ministry of Culture and Innovation supported G. P. (UNKP-23-3-SZTE-497), A. S. (UNKP-23-3-SZTE-517), and J. P. V. (UNKP-23-3-SZTE-535). LA - English DB - MTMT ER - TY - JOUR AU - Sebőkné Nagy, Krisztina AU - Kóta, Zoltán AU - Kincses, András AU - Fazekas, Ákos Ferenc AU - Dér, András AU - László, Zsuzsanna AU - Páli, Tibor TI - Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 19 EP - 17 PG - 17 SN - 1420-3049 DO - 10.3390/molecules28196750 UR - https://m2.mtmt.hu/api/publication/34153921 ID - 34153921 N1 - Funding Agency and Grant Number: We would like to acknowledge the support provided by the National Research, Development, and Innovation Office of Hungary-PD-143268. [PD-143268]; National Research, Development, and Innovation Office of Hungary Funding text: We would like to acknowledge the support provided by the National Research, Development, and Innovation Office of Hungary-PD-143268. AB - The accumulation of proteins in filter membranes limits the efficiency of filtering technologies for cleaning wastewater. Efforts are ongoing to coat commercial filters with different materials (such as titanium dioxide, TiO2) to reduce the fouling of the membrane. Beyond monitoring the desired effect of the retention of biomolecules, it is necessary to understand what the biophysical changes are in water-soluble proteins caused by their interaction with the new coated filter membranes, an aspect that has received little attention so far. Using spin-label electron paramagnetic resonance (EPR), aided with native fluorescence spectroscopy and dynamic light scattering (DLS), here, we report the changes in the structure and dynamics of bovine serum albumin (BSA) exposed to TiO2 (P25) nanoparticles or passing through commercial polyvinylidene fluoride (PVDF) membranes coated with the same nanoparticles. We have found that the filtering process and prolonged exposure to TiO2 nanoparticles had significant effects on different regions of BSA, and denaturation of the protein was not observed, neither with the TiO2 nanoparticles nor when passing through the TiO2-coated filter membranes. LA - English DB - MTMT ER - TY - JOUR AU - Kincses, András AU - Vigh, Judit Piroska AU - Petrovszki, Dániel AU - Valkai, Sándor AU - Kocsis, Anna AU - Walter, Fruzsina AU - Lin, Hung-Yin AU - Jan, Jeng-Shiung AU - Deli, Mária Anna AU - Dér, András TI - The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions JF - BIOSENSORS J2 - BIOSENSORS-BASEL VL - 13 PY - 2023 IS - 3 PG - 17 SN - 2079-6374 DO - 10.3390/bios13030357 UR - https://m2.mtmt.hu/api/publication/33688148 ID - 33688148 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [NNE-129617, K-124922, PD-143268, SA-111/2021]; National Science Technology Council, Taiwan [NSTC107-2923-M-006-002-MY3] Funding text: The following funding was received from the National Research, Development and Innovation Office, Hungary: grants NNE-129617 (to M.A.D.), K-124922 (to A.D.), PD-143268 (to A.K.). F.R.W. was supported by the grant SA-111/2021 from Lorand Eotvos Research Network, Hungary. J.S.J. was supported by the National Science Technology Council, Taiwan: NSTC107-2923-M-006-002-MY3. AB - The application of lab-on-a-chip technologies in in vitro cell culturing swiftly resulted in improved models of human organs compared to static culture insert-based ones. These chip devices provide controlled cell culture environments to mimic physiological functions and properties. Models of the blood-brain barrier (BBB) especially profited from this advanced technological approach. The BBB represents the tightest endothelial barrier within the vasculature with high electric resistance and low passive permeability, providing a controlled interface between the circulation and the brain. The multi-cell type dynamic BBB-on-chip models are in demand in several fields as alternatives to expensive animal studies or static culture inserts methods. Their combination with integrated biosensors provides real-time and noninvasive monitoring of the integrity of the BBB and of the presence and concentration of agents contributing to the physiological and metabolic functions and pathologies. In this review, we describe built-in sensors to characterize BBB models via quasi-direct current and electrical impedance measurements, as well as the different types of biosensors for the detection of metabolites, drugs, or toxic agents. We also give an outlook on the future of the field, with potential combinations of existing methods and possible improvements of current techniques. LA - English DB - MTMT ER - TY - JOUR AU - Barabási, Beáta AU - Barna, Lilla AU - Santa Maria, Anaraquel AU - Harazin, András AU - Molnár, Réka AU - Kincses, András AU - Vigh, Judit Piroska AU - Dukay, Brigitta AU - Sántha, Miklós AU - Tóth, Erzsébet Melinda AU - Walter, Fruzsina AU - Deli, Mária Anna AU - Hoyk, Zsófia TI - Role of interleukin-6 and interleukin-10 in morphological and functional changes of the blood–brain barrier in hypertriglyceridemia JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 20 PY - 2023 IS - 1 PG - 20 SN - 2045-8118 DO - 10.1186/s12987-023-00418-3 UR - https://m2.mtmt.hu/api/publication/33688118 ID - 33688118 N1 - Funding Agency and Grant Number: ELKH Biological Research Center - National Research, Development, and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00060]; European Training Network [H2020-MSCA-ITN-2015, 675619] Funding text: Open access funding provided by ELKH Biological Research Center. This work was funded by the National Research, Development, and Innovation Office of Hungary, Grant Number GINOP-2.3.2-15-2016-00060. ARSM was supported by the European Training Network H2020-MSCA-ITN-2015 [Grant Number 675619]. LA - English DB - MTMT ER - TY - JOUR AU - Walter, Fruzsina AU - Harazin, András AU - Tóth, Andrea AU - Veszelka, Szilvia AU - Santa Maria, Anaraquel AU - Barna, Lilla AU - Kincses, András AU - Biczo, G AU - Balla, Zsolt AU - Kui, Balázs AU - Maléth, József AU - Cervenak, László AU - Tubak, Vilmos AU - Kittel, Ágnes AU - Rakonczay, Zoltán AU - Deli, Mária Anna TI - Blood–brain barrier dysfunction in l-ornithine induced acute pancreatitis in rats and the direct effect of l-ornithine on cultured brain endothelial cells JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 19 PY - 2022 IS - 1 PG - 20 SN - 2045-8118 DO - 10.1186/s12987-022-00308-0 UR - https://m2.mtmt.hu/api/publication/32667372 ID - 32667372 N1 - Institute of Biophysics, Biological Research Centre, Temesvári krt. 62, Szeged, 6726, Hungary Department of Medicine, University of Szeged, Kálvária sgt 57, Szeged, 6725, Hungary Department of Pathophysiology, University of Szeged, Semmelweis u. 1, Szeged, 6701, Hungary HAS-USZ Momentum Epithelial Cell Signaling and Secretion Research Group, University of Szeged, Dóm sqr. 10, Szeged, 6720, Hungary HCEMM-SZTE Molecular Gastroenterology Research Group, University of Szeged, Dóm sqr. 10, Szeged, 6720, Hungary Department of Internal Medicine and Hematology, Research Laboratory, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary Creative Laboratory Ltd, Temesvári krt. 62, Szeged, 6726, Hungary Institute of Experimental Medicine, Eötvös Loránd Research Network, Szigony u. 43, Budapest, 1083, Hungary Wyss Institute for Biologically Inspired Engineering at Harvard University, 3 Blackfan Circle, Boston, MA 02115, United States Department of Biomedicine, Faculty of Health, Aarhus University, Høegh-Guldbergs Gade 10, Aarhus C, 8000, Denmark Institute of Applied Sciences, Department of Environmental Biology and Education, Juhász Gyula Faculty of Education, University of Szeged, Boldogasszony sgt. 6, Szeged, 6725, Hungary Cited By :1 Export Date: 23 November 2022 Correspondence Address: Deli, M.A.; Institute of Biophysics, Temesvári krt. 62, Hungary; email: deli.maria@brc.hu LA - English DB - MTMT ER - TY - GEN ED - Dér, András ED - Valkai, Sándor ED - Walter, Fruzsina ED - Kincses, András TI - Special Issue "Versatile Organ-on-a-Chip Devices" PY - 2021 UR - https://m2.mtmt.hu/api/publication/33017577 ID - 33017577 LA - English DB - MTMT ER - TY - THES AU - Kincses, András TI - Lab-on-a-chip tool for the investigation of biological barriers [Csiplaboratóriumi eszköz biológiai gátak modellezésére] PB - Szegedi Tudományegyetem (SZTE) PY - 2021 SP - 49 DO - 10.14232/phd.11024 UR - https://m2.mtmt.hu/api/publication/32868162 ID - 32868162 LA - English DB - MTMT ER - TY - JOUR AU - Vigh, Judit Piroska AU - Kincses, András AU - Ozgür, Burak AU - Walter, Fruzsina AU - Santa Maria, Anaraquel AU - Valkai, Sándor AU - Vastag, Monika AU - Neuhaus, Winfried AU - Brodin, Birger AU - Dér, András AU - Deli, Mária Anna TI - Transendothelial Electrical Resistance Measurement across the Blood–Brain Barrier: A Critical Review of Methods JF - MICROMACHINES J2 - MICROMACHINES-BASEL VL - 12 PY - 2021 IS - 6 PG - 18 SN - 2072-666X DO - 10.3390/mi12060685 UR - https://m2.mtmt.hu/api/publication/32077894 ID - 32077894 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA NNE 129617, PD-128480]; Lundbeck FoundationLundbeckfonden [R155-2013-14113]; European Union's Horizon 2020 research and innovation program Innovative Medicines Initiative (IMI) [807015] These authors contributed equally to this manuscript. (Vigh JP and Kincses A) LA - English DB - MTMT ER - TY - JOUR AU - Fazakas, Csilla AU - Kozma, Mihály AU - Molnár, Kinga AU - Kincses, András AU - Dér, András AU - Fejér, Adrienn AU - Horváth, Barnabás AU - Wilhelm, Imola Mária AU - Krizbai, István Adorján AU - Végh, Attila Gergely TI - Breast adenocarcinoma-derived exosomes lower first-contact de-adhesion strength of adenocarcinoma cells to brain endothelial layer JF - COLLOIDS AND SURFACES B: BIOINTERFACES J2 - COLLOID SURFACE B VL - 204 PY - 2021 PG - 8 SN - 0927-7765 DO - 10.1016/j.colsurfb.2021.111810 UR - https://m2.mtmt.hu/api/publication/32008069 ID - 32008069 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00037, GINOP-2.3.2-15-2016-00020]; National Science Fund of HungaryOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [OTKA PD121130, FK128654, FK124114, K135425, K135475]; Romanian Ministry of Education and Research [PN-III-P1-1.1TE-2019-1302, PN-III-P4-ID-PCE-2020-1529]; Janos Bolyai fellowship of the Hungarian Academy of Sciences [BO/00213/19/8]; New National Excellence Program of the Ministry for Innovation and Technology of Hungary [UNKP-20-4-SZTE-138] Funding text: This work was supported by the National Research, Development and Innovation Office of Hungary in the framework of the GINOP2.3.215201600037, GINOP2.3.215201600020 programs, the National Science Fund of Hungary OTKA PD121130, FK128654, FK124114, K135425, K135475 and by the Romanian Ministry of Education and Research within the frame of PNIIIP11.1TE20191302, PN-III-P4-ID-PCE-2020-1529 (UEFISCDI) programs. Csilla Fazakas was supported by the Janos Bolyai fellowship (BO/00213/19/8) of the Hungarian Academy of Sciences. Kinga Molnar was supported by New National Excellence Program of the Ministry for Innovation and Technology of Hungary (uNKP204SZTE138) . LA - English DB - MTMT ER -