TY - JOUR AU - Hetényi, Anasztázia AU - Imre, Norbert AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Fehérje méretű molekulák humán sejtekbe juttatása lipid-raft mediált endocitózissal JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 45 PY - 2021 IS - 4 SP - 67 EP - 83 PG - 17 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/32570862 ID - 32570862 N1 - Nincs jelölve levelező szerzőség a közleményen. (BÉ SZTE admin5) LA - Hungarian DB - MTMT ER - TY - JOUR AU - Akel, Hussein AU - Pannonhalminé Csóka, Ildikó AU - Ambrus, Rita AU - Bocsik, Alexandra AU - Gróf, Ilona AU - Mészáros, Mária AU - Szecskó, Anikó AU - Kozma, Gábor AU - Veszelka, Szilvia AU - Deli, Mária Anna AU - Kónya, Zoltán AU - Katona, Gábor TI - In Vitro Comparative Study of Solid Lipid and PLGA Nanoparticles Designed to Facilitate Nose-to-Brain Delivery of Insulin JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 24 PG - 22 SN - 1661-6596 DO - 10.3390/ijms222413258 UR - https://m2.mtmt.hu/api/publication/32531953 ID - 32531953 N1 - Funding Agency and Grant Number: Ministry of Human Capacities, Hungary [TKP-2020]; National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP2.3.2-15-2016-00060] Funding text: The publication was founded by the Ministry of Human Capacities, Hungary (Grant TKP-2020), and by the National Research, Development and Innovation Office, Hungary (GINOP2.3.2-15-2016-00060) projects. LA - English DB - MTMT ER - TY - JOUR AU - Bíró, Tivadar AU - Bocsik, Alexandra AU - Jurišić Dukovski, Bisera AU - Gróf, Ilona AU - Lovrić, Jasmina AU - Pannonhalminé Csóka, Ildikó AU - Deli, Mária Anna AU - Aigner, Zoltán TI - New Approach in Ocular Drug Delivery. In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations TS - In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations JF - DRUG DESIGN DEVELOPMENT AND THERAPY J2 - DRUG DES DEV THER VL - 15 PY - 2021 SP - 351 EP - 360 PG - 10 SN - 1177-8881 DO - 10.2147/DDDT.S264745 UR - https://m2.mtmt.hu/api/publication/31890902 ID - 31890902 AB - Background: Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye. Antimicrobial stability of topical ophthalmic formulations is especially important. According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required. In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects. Methods: The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model. The lipophilic drug is dissolved by the formation of cyclodextrin inclusion complex. Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride. Results: As the results show, benzalkonium-chloride-containing samples were toxic on HCE-T cells. The biopolymer caused no cell damage after the treatment. This was confirmed by immunohistochemistry assay. The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation. According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability. Conclusion: Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration. It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability. LA - English DB - MTMT ER - TY - JOUR AU - Bíró, Tivadar AU - Bocsik, Alexandra AU - Gróf, Ilona AU - Bisera, Jurisic Dukowski AU - Jasmina, Lovric AU - Deli, Mária Anna AU - Aigner, Zoltán TI - Investigation of the permeability and cytotoxicity in novel topical ophthalmic formulations using in vitro and ex vivo models JF - ACTA PHARMACEUTICA HUNGARICA J2 - ACTA PHARM HUNG VL - 90 PY - 2020 IS - 2-3 SP - 86 EP - 86 PG - 1 SN - 0001-6659 UR - https://m2.mtmt.hu/api/publication/31978759 ID - 31978759 LA - English DB - MTMT ER - TY - JOUR AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Harazin, András AU - Santa Maria, Anaraquel AU - Vizsnyiczai, Gaszton AU - Barna, Lilla AU - Kiss, Lóránd AU - Fűr, Gabriella AU - Rakonczay, Zoltán AU - Ambrus, Rita AU - Révész, Piroska AU - Gosselet, Fabien AU - Pongsiri, Jaikumpun AU - Szabó, Hajnalka AU - Zsembery, Ákos AU - Deli, Mária Anna TI - The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 11 PG - 23 SN - 1661-6596 DO - 10.3390/ijms21114024 UR - https://m2.mtmt.hu/api/publication/31335512 ID - 31335512 LA - English DB - MTMT ER - TY - JOUR AU - Barna, Lilla AU - Walter, Fruzsina AU - Harazin, András AU - Bocsik, Alexandra AU - Kincses, András AU - Tubak, Vilmos AU - Jósvay, Katalin AU - Zvara, Ágnes AU - Campos-Bedolla, Patricia AU - Deli, Mária Anna TI - Simvastatin, edaravone and dexamethasone protect against kainate-induced brain endothelial cell damage. JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 17 PY - 2020 IS - 1 PG - 13 SN - 2045-8118 DO - 10.1186/s12987-019-0166-1 UR - https://m2.mtmt.hu/api/publication/31181444 ID - 31181444 N1 - Lilla Barna, Fruzsina R. Walter and András Harazin contributed equally to the manuscript. AB - Excitotoxicity is a central pathological pathway in many neurological diseases with blood-brain barrier (BBB) dysfunction. Kainate, an exogenous excitotoxin, induces epilepsy and BBB damage in animal models, but the direct effect of kainate on brain endothelial cells has not been studied in detail. Our aim was to examine the direct effects of kainate on cultured cells of the BBB and to test three anti-inflammatory and antioxidant drugs used in clinical practice, simvastatin, edaravone and dexamethasone, to protect against kainate-induced changes.Primary rat brain endothelial cell, pericyte and astroglia cultures were used to study cell viability by impedance measurement. BBB permeability was measured on a model made from the co-culture of the three cell types. The production of nitrogen monoxide and reactive oxygen species was followed by fluorescent probes. The mRNA expression of kainate receptors and nitric oxide synthases were studied by PCR.Kainate damaged brain endothelial cells and made the immunostaining of junctional proteins claudin-5 and zonula occludens-1 discontinuous at the cell border indicating the opening of the barrier. The permeability of the BBB model for marker molecules fluorescein and albumin and the production of nitric oxide in brain endothelial cells were increased by kainate. Simvastatin, edaravone and dexamethasone protected against the reduced cell viability, increased permeability and the morphological changes in cellular junctions caused by kainate. Dexamethasone attenuated the elevated nitric oxide production and decreased the inducible nitric oxide synthase (NOS2/iNOS) mRNA expression increased by kainate treatment.Kainate directly damaged cultured brain endothelial cells. Simvastatin, edaravone and dexamethasone protected the BBB model against kainate-induced changes. Our results confirmed the potential clinical usefulness of these drugs to attenuate BBB damage. LA - English DB - MTMT ER - TY - JOUR AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Routing Nanomolar Protein Cargoes to Lipid Raft‐Mediated/Caveolar Endocytosis through a Ganglioside GM1‐Specific Recognition Tag JF - ADVANCED SCIENCE J2 - ADV SCI VL - 7 PY - 2020 IS - 4 PG - 10 SN - 2198-3844 DO - 10.1002/advs.201902621 UR - https://m2.mtmt.hu/api/publication/31126947 ID - 31126947 N1 - Funding text: This research was funded by the National Research, Development and Innovation Office of Hungary, grant number GINOP-2.2.1-15-2016-00007, the Hungarian Ministry of Innovation and Technology, TUDFO/47138-1/2019-ITM, and Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG). T.A.M. acknowledges support from the Hungarian Academy of Sciences LENDULET-Foldamer. P.H. acknowledges support from the Finnish TEKES FiDiPro Fellow Grant 40294/13, the Hungarian Academy of Sciences LENDULET-Biomag. LA - English DB - MTMT ER - TY - JOUR AU - Ismail, Ruba AU - Bocsik, Alexandra AU - Katona, Gábor AU - Gróf, Ilona AU - Deli, Mária Anna AU - Pannonhalminé Csóka, Ildikó TI - Encapsulation in Polymeric Nanoparticles Enhances the Enzymatic Stability and the Permeability of the GLP-1 Analog, Liraglutide, Across a Culture Model of Intestinal Permeability JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 11 PY - 2019 IS - 11 PG - 13 SN - 1999-4923 DO - 10.3390/pharmaceutics11110599 UR - https://m2.mtmt.hu/api/publication/30912242 ID - 30912242 LA - English DB - MTMT ER - TY - JOUR AU - Bocsik, Alexandra AU - Gróf, Ilona AU - Kiss, Lóránd AU - Ötvös, Ferenc AU - Zsíros, Ottó AU - Daruka, Lejla AU - Fülöp, Lívia AU - Vastag, Monika AU - Kittel, Ágnes AU - Imre, Norbert AU - Martinek, Tamás AU - Pál, Csaba AU - Révész, Piroska AU - Deli, Mária Anna TI - Dual Action of the PN159/KLAL/MAP Peptide. Increase of Drug Penetration across Caco-2 Intestinal Barrier Model by Modulation of Tight Junctions and Plasma Membrane Permeability. TS - Increase of Drug Penetration across Caco-2 Intestinal Barrier Model by Modulation of Tight Junctions and Plasma Membrane Permeability. JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 11 PY - 2019 IS - 2 PG - 21 SN - 1999-4923 DO - 10.3390/pharmaceutics11020073 UR - https://m2.mtmt.hu/api/publication/30434125 ID - 30434125 AB - The absorption of drugs is limited by the epithelial barriers of the gastrointestinal tract. One of the strategies to improve drug delivery is the modulation of barrier function by the targeted opening of epithelial tight junctions. In our previous study the 18-mer amphiphilic PN159 peptide was found to be an effective tight junction modulator on intestinal epithelial and blood⁻brain barrier models. PN159, also known as KLAL or MAP, was described to interact with biological membranes as a cell-penetrating peptide. In the present work we demonstrated that the PN159 peptide as a penetration enhancer has a dual action on intestinal epithelial cells. The peptide safely and reversibly enhanced the permeability of Caco-2 monolayers by opening the intercellular junctions. The penetration of dextran molecules with different size and four efflux pump substrate drugs was increased several folds. We identified claudin-4 and -7 junctional proteins by docking studies as potential binding partners and targets of PN159 in the opening of the paracellular pathway. In addition to the tight junction modulator action, the peptide showed cell membrane permeabilizing and antimicrobial effects. This dual action is not general for cell-penetrating peptides (CPPs), since the other three CPPs tested did not show barrier opening effects. LA - English DB - MTMT ER - TY - JOUR AU - Bíró, Tivadar AU - Horvát, Gabriella AU - Bocsik, Alexandra AU - Gróf, Ilona AU - Zsoldiné Urbán, Edit AU - Deli, Mária Anna AU - Csányi, Erzsébet AU - Révész, Piroska AU - Pannonhalminé Csóka, Ildikó AU - Aigner, Zoltán TI - Formulation of steroid containing eye drops with cyclodextrin derivatives and mucoadhesive preservative system JF - ACTA PHARMACEUTICA HUNGARICA J2 - ACTA PHARM HUNG VL - 88 PY - 2018 IS - 3 SP - 139 EP - 140 PG - 2 SN - 0001-6659 UR - https://m2.mtmt.hu/api/publication/30548713 ID - 30548713 LA - English DB - MTMT ER -