TY - CONF AU - Rauch, Enikő AU - Kovács, Zsolt ED - Puskás, János TI - A keton táplálékkiegészítők izoflurán által indukált vércukorszint-emelkedésre gyakorolt hatása hím és nőstény WAG/Rij patkányokban T2 - Program és az előadások összefoglalói C1 - Szombathely PY - 2024 SP - 4 EP - 4 PG - 1 UR - https://m2.mtmt.hu/api/publication/34526563 ID - 34526563 N1 - Előadás LA - Hungarian DB - MTMT ER - TY - GEN AU - Ari, Csilla AU - D’Agostino, Dominic P. AU - Kovács, Zsolt TI - Review of exogenous ketone supplements with enantiomer and concentration dependent effects PY - 2023 UR - https://m2.mtmt.hu/api/publication/34204869 ID - 34204869 N1 - Poszter LA - English DB - MTMT ER - TY - JOUR AU - Rauch, Enikő AU - Ari, Csilla AU - Kovács, Zsolt TI - Dose-Dependent Beneficial Effect of Ketone Supplement-Evoked Ketosis on Anxiety Level in Female WAG/Rij Rats: Sometimes Less Is More JF - NUTRIENTS J2 - NUTRIENTS VL - 15 PY - 2023 IS - 20 PG - 12 SN - 2072-6643 DO - 10.3390/nu15204412 UR - https://m2.mtmt.hu/api/publication/34204821 ID - 34204821 AB - While one-third of the population can be affected by anxiety disorders during their lifetime, our knowledge of the pathophysiology of these disorders is far from complete. Previously, it has been demonstrated in male animals that exogenous ketone supplement-evoked ketosis can decrease anxiety levels in preclinical rodent models, such as Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats. Thus, in this study, we investigated whether intragastric gavage of the exogenous ketone supplement KEMCT (mix of 1,3-butanediol-acetoacetate diester/ketone ester/KE and medium-chain triglyceride/MCT oil in 1:1 ratio) for 7 days can alter the anxiety levels of female WAG/Rij rats using the light–dark box (LDB) test. We demonstrated that a lower dose of KEMCT (3 g/kg/day) increased blood R-βHB (R-β-hydroxybutyrate) levels and significantly decreased anxiety levels (e.g., increased the time spent in the light compartment) in female WAG/Rij rats on the seventh day of administration. Although the higher KEMCT dose (5 g/kg/day) increased blood R-βHB levels more effectively, compared with the lower KEMCT dose, anxiety levels did not improve significantly. We conclude that ketone supplementation might be an effective strategy to induce anxiolytic effects not only in male but also in female WAG/Rij rats. However, these results suggest that the optimal level may be moderately, not highly, elevated blood R-βHB levels when the goal is to alleviate symptoms of anxiety. More studies are needed to understand the exact mechanism of action of ketone supplementation on anxiety levels and to investigate their use in other animal models and humans for the treatment of anxiety disorders and other mental health conditions. LA - English DB - MTMT ER - TY - GEN AU - Brunner, Brigitta AU - Kovács, Zsolt TI - Az adenozin receptorok modulátoros hatása a keton táplálékkiegészítők kiváltotta rohamszám csökkenésben WAG/Rij patkányokban. PY - 2023 UR - https://m2.mtmt.hu/api/publication/33669033 ID - 33669033 N1 - előadás LA - Hungarian DB - MTMT ER - TY - CONF AU - Saif, Qahtan AU - Márton, Péter AU - Kovács, Zsolt AU - Serguei, Skatchkov AU - Zsolt, Szabó AU - Julianna, Kardos AU - László, Héja TI - Antiepileptic effect of the GABA precursor putrescine in early termination of seizures. T2 - Joint Neuroscience Meeting of the Hungarian Neuroscience Society (MITT) & the Austrian Neuroscience Association (ANA) PY - 2023 SP - 63 UR - https://m2.mtmt.hu/api/publication/33669006 ID - 33669006 N1 - poszter LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zsolt AU - D’Agostino, Dominic P. AU - Ari, Csilla TI - Ketone supplementation abolished isoflurane anesthesia-induced elevation in blood glucose level and increased recovery time from anesthesia in Wistar Albino Glaxo Rijswijk rats JF - BMC ANESTHESIOLOGY J2 - BMC ANESTHESIOL VL - 23 PY - 2023 IS - 1 PG - 9 SN - 1471-2253 DO - 10.1186/s12871-023-02000-8 UR - https://m2.mtmt.hu/api/publication/33668972 ID - 33668972 N1 - Savaria Department of Biology, ELTE Eötvös Loránd University, Savaria University Centre, Szombathely, Hungary Ketone Technologies LLC, Tampa, FL, United States Department of Molecular Pharmacology and Physiology, Laboratory of Metabolic Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, United States Institute for Human and Machine Cognition, Ocala, FL, United States Department of Psychology, Behavioral Neuroscience Research Laboratory, University of South Florida, 4202 E. Fowler Ave, PCD 3127, Tampa, FL, United States Export Date: 2 March 2023 CODEN: BAMNB Correspondence Address: Ari, C.; Ketone Technologies LLCUnited States; email: csari2000@yahoo.com AB - Background It has been suggested that administration of exogenous ketone supplements (EKSs) not only increases blood ketone body levels but also decreases blood glucose level and modulates isoflurane-induced anesthesia in different rodents, such as Wistar Albino Glaxo Rijswijk (WAG/Rij) rats. Thus, we investigated whether administration of EKSs can modulate the isoflurane anesthesia-generated increase in blood glucose level and the time required to recover from isoflurane-induced anesthesia. Methods To investigate the effect of EKSs on isoflurane anesthesia-induced changes in blood glucose and R-β-hydroxybutyrate (R-βHB) level as well as recovery time from anesthesia, we used KEMCT (mix of ketone ester/KE and medium chain triglyceride/MCT oil in a 1:1 ratio) in WAG/Rij rats. First, to accustom the animals to the method, water gavage was carried out for 5 days (adaptation period). After adaptation period, rats of first group (group 1) were gavaged by water (3 g/kg), whereas, in the case of second group (group 2), the diet of animals was supplemented by KEMCT (3 g/kg, gavage) once per day for 7 days. One hour after the last gavage, isoflurane (3%) anesthesia was induced for 20 min (group 1 and group 2) and the time required for recovery from anesthesia was measured by using righting reflex. Subsequently, blood levels of both R-βHB and glucose were also evaluated. Changes in blood glucose and R-βHB levels were compared to control, which control glucose and R-βHB levels were measured on the last day of the adaptation period (group 1 and group 2). Time required for recovery from isoflurane anesthesia, which was detected after 7th KEMCT gavage (group 2), was compared to recovery time measured after 7th water gavage (group 1). Results The KEMCT maintained the normal glucose level under isoflurane anesthesia-evoked circumstances preventing the glucose level elevating effect of isoflurane. Thus, we demonstrated that administration of KEMCT not only increased blood level of R-βHB but also abolished the isoflurane anesthesia-generated increase in blood glucose level. Moreover, the time required for recovery from isoflurane-evoked anesthesia increased significantly in KEMCT treated animals. Conclusions Putative influence of elevated blood ketone body level on isoflurane-evoked effects, such as modulation of blood glucose level and recovery time from anesthesia, should be considered by anesthesiologists. LA - English DB - MTMT ER - TY - CONF AU - Kovács, Zsolt TI - Influence of ketone supplements on epileptic seizures and anxiety in WAG/Rij rats T2 - ESMED 2022 GENERAL ASSEMBY ABSTRACT BOOK PY - 2022 SP - 138 EP - 139 PG - 2 UR - https://m2.mtmt.hu/api/publication/33212674 ID - 33212674 N1 - Előadás: European Society of Medicine's General Assembly, August 4-6., 2022; LA - English DB - MTMT ER - TY - CONF AU - Kovács, Zsolt TI - Effect of ketone supplements on absence epileptic seizures in the WAG/Rij rat model T2 - World Brain Disorders and Neuroscience Summit 2022 PY - 2022 SP - 14 EP - 14 PG - 1 UR - https://m2.mtmt.hu/api/publication/33212671 ID - 33212671 N1 - Előadás LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zsolt AU - Skatchkov, Serguei N. AU - Szabó, Zsolt AU - Qahtan, Saif AU - Méndez-González, Miguel P. AU - Malpica-Nieves, Christian J. AU - Eaton, Misty J. AU - Kardos, Julianna AU - Héja, László TI - Putrescine Intensifies Glu/GABA Exchange Mechanism and Promotes Early Termination of Seizures JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 15 PG - 11 SN - 1661-6596 DO - 10.3390/ijms23158191 UR - https://m2.mtmt.hu/api/publication/33031938 ID - 33031938 N1 - Department of Biology, Savaria University Centre, ELTE Eötvös Loránd University, Károlyi Gáspár tér 4, Szombathely, 9700, Hungary Department of Physiology, Universidad Central del Caribe, Bayamon, 00960, Puerto Rico Department of Biochemistry, Universidad Central del Caribe, Bayamon, 00960, Puerto Rico Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, Budapest, 1117, Hungary School of Chemistry, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, 1117, Hungary College of Science, University of Al-Qadisiyah, Al-Diwaniyah, 58001, Iraq Natural Sciences Department, University of Puerto Rico in Aguadilla, Aguadilla, 00604, Puerto Rico Department of Science and Technology, Antilles Adventist University, Mayagüez, 00681, Puerto Rico Export Date: 25 October 2022 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Magyar Tudósok Körútja 2, Hungary; email: heja.laszlo@ttk.hu AB - Endogenous anticonvulsant mechanisms represent a reliable and currently underdeveloped strategy against recurrent seizures and may recall novel original therapeutics. Here, we investigated whether the intensification of the astroglial Glu-GABA exchange mechanism by application of the GABA precursor putrescine (PUT) may be effective against convulsive and non-convulsive seizures. We explored the potential of PUT to inhibit spontaneous spike-and-wave discharges (SWDs) in WAG/Rij rats, a genetic model of absence epilepsy. Significant shortening of SWDs in response to intraperitoneally applied PUT has been observed, which could be antagonized by blocking GAT-2/3-mediated astrocytic GABA release with the specific inhibitor SNAP-5114. Direct application of exogenous GABA also reduced SWD duration, suggesting that PUT-triggered astroglial GABA release through GAT-2/3 may be a critical step in limiting seizure duration. PUT application also dose-dependently shortened seizure-like events (SLEs) in the low-[Mg2+] in vitro model of temporal lobe epilepsy. SNAP-5114 reversed the antiepileptic effect of PUT in the in vitro model as well, further confirming that PUT reduces seizure duration by triggering glial GABA release. In accordance, we observed that PUT specifically reduces the frequency of excitatory synaptic potentials, suggesting that it specifically acts at excitatory synapses. We also identified that PUT specifically eliminated the tonic depolarization-induced desynchronization of SLEs. Since PUT is an important source of glial GABA and we previously showed significant GABA release, it is suggested that the astroglial Glu-GABA exchange mechanism plays a key role in limiting ictal discharges, potentially opening up novel pathways to control seizure propagation and generalization. LA - English DB - MTMT ER - TY - GEN AU - Csilla, Ari AU - Collin, Park AU - John, Vallas AU - Raquel, Sanford AU - Sahil, Bharwani AU - Dominic, P. D’Agostino AU - Kovács, Zsolt TI - Concentration Dependent Effect of Ketone Bodies: Cell, Animal and Human Studies - Higher is Not Always Better PY - 2022 UR - https://m2.mtmt.hu/api/publication/32897157 ID - 32897157 N1 - Poszter LA - English DB - MTMT ER -