@article{MTMT:34840271,
	title = {Peripheral immunophenotyping reveals lymphocyte stimulation in healthy women living with hereditary breast and ovarian cancer syndrome},
	url = {https://m2.mtmt.hu/api/publication/34840271},
	author = {Balog, József Ágoston and Horti-Oravecz, Klaudia and Kövesdi, Dorottya and Bozsik, Anikó and Papp, Janos and Butz, Henriett and Patócs, Attila and Szebeni, Gábor and Grolmusz, Vince Kornél},
	doi = {10.1016/j.isci.2024.109882},
	journal-iso = {ISCIENCE},
	journal = {ISCIENCE},
	volume = {AiP},
	unique-id = {34840271},
	year = {2024},
	eissn = {2589-0042},
	pages = {109882},
	orcid-numbers = {Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34840255,
	title = {Identification of immune subsets with distinct lectin binding signatures using multi-parameter flow cytometry: correlations with disease activity in systemic lupus erythematosus},
	url = {https://m2.mtmt.hu/api/publication/34840255},
	author = {Szabó, Enikő and Faragó, Anna and Bodor, Gergely and Gémes, Nikolett and Puskás, László and Kovács, László and Szebeni, Gábor},
	doi = {10.3389/fimmu.2024.1380481},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {15},
	unique-id = {34840255},
	issn = {1664-3224},
	year = {2024},
	eissn = {1664-3224},
	orcid-numbers = {Kovács, László/0000-0003-4457-1430; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34829278,
	title = {Comparative single-cell multiplex immunophenotyping of therapy-naive patients with rheumatoid arthritis, systemic sclerosis, and systemic lupus erythematosus shed light on disease-specific composition of the peripheral immune system},
	url = {https://m2.mtmt.hu/api/publication/34829278},
	author = {Balog, József Ágoston and Zvara, Ágnes and Bukovinszki, Vivien and Puskás, László and Balog, Attila and Szebeni, Gábor},
	doi = {10.3389/fimmu.2024.1376933},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {15},
	unique-id = {34829278},
	issn = {1664-3224},
	year = {2024},
	eissn = {1664-3224},
	orcid-numbers = {Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34790193,
	title = {Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model},
	url = {https://m2.mtmt.hu/api/publication/34790193},
	author = {Faragó, Anna and Zvara, Ágnes and Tiszlavicz, László and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Hegedűs, Zoltán and Szabó, Enikő and Surguta, Sára Eszter and Tóvári, József and Puskás, László and Szebeni, Gábor},
	doi = {10.3390/ijms25074022},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34790193},
	issn = {1661-6596},
	abstract = {A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies.},
	keywords = {colorectal carcinoma; lectin binding sugar code; proteomic analysis of murine CRC},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Tiszlavicz, László/0000-0003-1134-6587; Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34486293,
	title = {Single-cell immunophenotyping revealed the association of CD4+ central and CD4+ effector memory T cells linking exacerbating chronic obstructive pulmonary disease and NSCLC.},
	url = {https://m2.mtmt.hu/api/publication/34486293},
	author = {Gémes, Nikolett and Balog, József Ágoston and Neuperger, Patricia and Schlegl, Erzsébet and Barta, Imre and Fillinger, János and Antus, Balázs and Zvara, Ágnes and Hegedűs, Zoltán and Czimmerer, Zsolt and Manczinger, Máté and Balogh, Gergő Mihály and Tóvári, József and Puskás, László and Szebeni, Gábor},
	doi = {10.3389/fimmu.2023.1297577},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {14},
	unique-id = {34486293},
	issn = {1664-3224},
	abstract = {Tobacco smoking generates airway inflammation in chronic obstructive pulmonary disease (COPD), and its involvement in the development of lung cancer is still among the leading causes of early death. Therefore, we aimed to have a better understanding of the disbalance in immunoregulation in chronic inflammatory conditions in smoker subjects with stable COPD (stCOPD), exacerbating COPD (exCOPD), or non-small cell lung cancer (NSCLC).Smoker controls without chronic illness were recruited as controls. Through extensive mapping of single cells, surface receptor quantification was achieved by single-cell mass cytometry (CyTOF) with 29 antibodies. The CyTOF characterized 14 main immune subsets such as CD4+, CD8+, CD4+/CD8+, CD4-/CD8-, and γ/δ T cells and other subsets such as CD4+ or CD8+ NKT cells, NK cells, B cells, plasmablasts, monocytes, CD11cdim, mDCs, and pDCs. The CD4+ central memory (CM) T cells (CD4+/CD45RA-/CD45RO+/CD197+) and CD4+ effector memory (EM) T cells (CD4+/CD45RA-/CD45RO+/CD197-) were FACS-sorted for RNA-Seq analysis. Plasma samples were assayed by Luminex MAGPIX® for the quantitative measurement of 17 soluble immuno-oncology mediators (BTLA, CD28, CD80, CD27, CD40, CD86, CTLA-4, GITR, GITRL, HVEM, ICOS, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2) in the four studied groups.Our focus was on T-cell-dependent differences in COPD and NSCLC, where peripheral CD4+ central memory and CD4+ effector memory cells showed a significant reduction in exCOPD and CD4+ CM showed elevation in NSCLC. The transcriptome analysis delineated a perfect correlation of differentially expressed genes between exacerbating COPD and NSCLC-derived peripheral CD4+ CM or CD4+ EM cells. The measurement of 17 immuno-oncology soluble mediators revealed a disease-associated phenotype in the peripheral blood of stCOPD, exCOPD, and NSCLC patients.The applied single-cell mass cytometry, the whole transcriptome profiling of peripheral CD4+ memory cells, and the quantification of 17 plasma mediators provided complex data that may contribute to the understanding of the disbalance in immune homeostasis generated or sustained by tobacco smoking in COPD and NSCLC.},
	keywords = {Tobacco smoking; non-small cell lung cancer; single-cell mass cytometry; CD4 central memory T cells; CD4 effector memory T cells; exacerbating COPD; stable COPD},
	year = {2023},
	eissn = {1664-3224},
	orcid-numbers = {Manczinger, Máté/0000-0003-0831-9617; Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34199166,
	title = {Single-cell mass cytometric analysis of peripheral immunity and multiplex plasma marker profiling of non-small cell lung cancer patients receiving PD-1 targeting immune checkpoint inhibitors in comparison with platinum-based chemotherapy},
	url = {https://m2.mtmt.hu/api/publication/34199166},
	author = {Neuperger, Patricia and Szalontai, Klára Margit and Gémes, Nikolett and Balog, József Ágoston and Tiszlavicz, László and Furák, József and Lázár, György ifj and Puskás, László and Szebeni, Gábor},
	doi = {10.3389/fimmu.2023.1243233},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {14},
	unique-id = {34199166},
	issn = {1664-3224},
	year = {2023},
	eissn = {1664-3224},
	orcid-numbers = {Tiszlavicz, László/0000-0003-1134-6587; Furák, József/0000-0002-7224-1642; Lázár, György ifj/0000-0001-7155-2978; Szebeni, Gábor/0000-0002-6998-5632}
}

@{MTMT:34136606,
	title = {A PCOS gyulladásos hátterének vizsgálata és terápiás megközelítése},
	url = {https://m2.mtmt.hu/api/publication/34136606},
	author = {Börzsei, Denise and Nagyné Hoffmann, Alexandra and Kiss, Viktória and Sugár, Anna and Neuperger, Patrícia and Szebeni, Gábor and Bagyánszki, Mária and Barta, Bence Pál and Almási, Nikoletta and Veszelka, Médea and Török, Szilvia and Varga, Csaba and Szabó, Renáta},
	booktitle = {A Magyar Szabadgyökkutató Társaság XII. Kongresszusa},
	unique-id = {34136606},
	year = {2023},
	pages = {16-16},
	orcid-numbers = {Szebeni, Gábor/0000-0002-6998-5632; Bagyánszki, Mária/0000-0003-3533-9461; Barta, Bence Pál/0000-0002-5309-8633; Almási, Nikoletta/0000-0002-7371-5272; Varga, Csaba/0000-0002-2678-665X}
}

@article{MTMT:34131836,
	title = {Antiproliferative and Antimetastatic Properties of 16-Azidomethyl Substituted 3-O-Benzyl Estrone Analogs},
	url = {https://m2.mtmt.hu/api/publication/34131836},
	author = {Senobar Tahaei, Seyyed Ashkan and Kulmány, Ágnes Erika and Minorics, Renáta and Kiss, Anita and Szabó, Zoltán and Germán, Péter and Szebeni, Gábor and Gémes, Nikolett and Mernyák, Erzsébet and Zupkó, István},
	doi = {10.3390/ijms241813749},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {24},
	unique-id = {34131836},
	issn = {1661-6596},
	abstract = {Four diastereomers of 16-azidomethyl substituted 3-O-benzyl estradiol (1–4) and their two estrone analogs (16AABE and 16BABE) were tested for their antiproliferative properties against human gynecological cancer cell lines. The estrones were selected for additional experiments based on their outstanding cell growth-inhibiting activities. Both compounds increased hypodiploid populations of breast cancer cells, and 16AABE elicited cell cycle disturbance as evidenced by flow cytometry. The two analogs substantially increased the rate of tubulin polymerization in vitro. 16AABE and 16BABE inhibited breast cancer cells’ migration and invasive ability, as evidenced by wound healing and Boyden chamber assays. Since both estrone analogs exerted remarkable estrogenic activities, as documented by a luciferase reporter gene assay, they can be considered as promising drug candidates for hormone-independent malignancies.},
	year = {2023},
	eissn = {1422-0067},
	orcid-numbers = {Minorics, Renáta/0000-0001-9685-813X; Kiss, Anita/0000-0003-3352-0996; Szabó, Zoltán/0000-0001-8278-8038; Szebeni, Gábor/0000-0002-6998-5632; Mernyák, Erzsébet/0000-0003-4494-1817; Zupkó, István/0000-0003-3243-5300}
}

@article{MTMT:34093747,
	title = {Galectin-1 as a marker for microglia activation in the aging brain},
	url = {https://m2.mtmt.hu/api/publication/34093747},
	author = {Kiss, Tamás and Mir, Mohd Yaqub and Stefancsik, Gergely and Ganbat, Gantulga and Askarova, Aruzhan and Monostori, Éva and Dulka, Karolina and Szebeni, Gábor and Nyúl-Tóth, Ádám and Csiszar, Anna and Légrádi, Ádám},
	doi = {10.1016/j.brainres.2023.148517},
	journal-iso = {BRAIN RES},
	journal = {BRAIN RESEARCH},
	volume = {1818},
	unique-id = {34093747},
	issn = {0006-8993},
	abstract = {Microglia cells, the immune cells residing in the brain, express immune regulatory molecules that have a central role in the manifestation of age-related brain characteristics. Our hypothesis suggests that galectin-1, an anti-inflammatory member of the beta-galactoside-binding lectin family, regulates microglia and neuroinflammation in the aging brain. Through our in-silico analysis, we discovered a subcluster of microglia in the aged mouse brain that exhibited increased expression of galectin-1 mRNA. In our Western blotting experiments, we observed a decrease in galectin-1 protein content in our rat primary cortical cultures over time. Additionally, we found that the presence of lipopolysaccharide, an immune activator, significantly increased the expression of galectin-1 protein in microglial cells. Utilizing flow cytometry, we determined that a portion of the galectin-1 protein was localized on the surface of the microglial cells. As cultivation time increased, we observed a decrease in the expression of activation-coupled molecules in microglial cells, indicating cellular exhaustion. In our mixed rat primary cortical cell cultures, we noted a transition of amoeboid microglial cells labeled with OX42(CD11b/c) to a ramified, branched phenotype during extended cultivation, accompanied by a complete disappearance of galectin-1 expression. By analyzing the transcriptome of a distinct microglial subpopulation in an animal model of aging, we established a correlation between chronological aging and galectin-1 expression. Furthermore, our in vitro study demonstrated that galectin-1 expression is associated with the functional activation state of microglial cells exhibiting specific amoeboid morphological characteristics. Based on our findings, we identify galectin-1 as a marker for microglia activation in the context of aging.},
	keywords = {Aging; Galectin-1; neuroinflammation; microglia},
	year = {2023},
	eissn = {1872-6240},
	orcid-numbers = {Kiss, Tamás/0000-0001-5339-5227; Stefancsik, Gergely/0000-0002-2098-652X; Monostori, Éva/0000-0002-7442-3562; Dulka, Karolina/0000-0002-7368-8198; Szebeni, Gábor/0000-0002-6998-5632; Légrádi, Ádám/0000-0001-7994-1935}
}

@article{MTMT:34076543,
	title = {Introduction of an Ultraviolet C-Irradiated 4T1 Murine Breast Cancer Whole-Cell Vaccine Model},
	url = {https://m2.mtmt.hu/api/publication/34076543},
	author = {Szebeni, Gábor and Alföldi, Róbert and Nagy, Lajos I. and Neuperger, Patricia and Gémes, Nikolett and Balog, József Ágoston and Tiszlavicz, László and Puskás, László},
	doi = {10.3390/vaccines11071254},
	journal-iso = {VACCINES-BASEL},
	journal = {VACCINES (BASEL)},
	volume = {11},
	unique-id = {34076543},
	abstract = {The advent of immunotherapy has revolutionized cancer treatments. However, the application of immune checkpoint inhibitors may entail severe side effects, with the risk of therapeutic resistance. The generation of chimeric antigen receptor (CAR) T-cells or CAR-NK cells requires specialized molecular laboratories, is costly, and is difficult to adapt to the rapidly growing number of cancer patients. To provide a simpler but effective immune therapy, a whole-cell tumor vaccine protocol was established based on ultraviolet C (UCV)-irradiated 4T1 triple-negative breast cancer cells. The apoptosis of tumor cells after UVC irradiation was verified using resazurin and Annexin V/propidium iodide flow cytometric assays. Protective immunity was achieved in immunized BALB/c mice, showing partial remission. Adoptive transfer of splenocytes or plasma from the mice in remission showed a protective effect in the naive BALB/c mice that received a living 4T1 tumor cell injection. 4T1-specific IgG antibodies were recorded in the plasma of the mice following immunization with the whole-cell vaccine. Interleukin-2 (IL-2) and oligonucleotide 2006 (ODN2006) adjuvants were used for the transfer of splenocytes from C57BL/6 mice into cyclophosphamide-treated BALB/c mice, resulting in prolonged survival, reduced tumor growth, and remission in 33% of the cases, without the development of the graft-versus-host disease. Our approach offers a simple, cost-effective whole-cell vaccine protocol that can be administered to immunocompetent healthy organisms. The plasma or the adoptive transfer of HLA-matching immunized donor-derived leukocytes could be used as an immune cell therapy for cancer patients.},
	year = {2023},
	eissn = {2076-393X},
	orcid-numbers = {Szebeni, Gábor/0000-0002-6998-5632; Tiszlavicz, László/0000-0003-1134-6587}
}