TY - JOUR AU - Laurinyecz, Barbara AU - Vedelek, Viktor AU - Kovács, Attila Lajos AU - Szilasi, Kinga AU - Lipinszki, Zoltán AU - Slezák, Csilla AU - Darula, Zsuzsanna AU - Juhász, Gábor AU - Sinka, Rita TI - Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm. JF - PLOS GENETICS J2 - PLOS GENET VL - 15 PY - 2019 IS - 2 PG - 24 SN - 1553-7390 DO - 10.1371/journal.pgen.1007987 UR - https://m2.mtmt.hu/api/publication/30481567 ID - 30481567 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00032, 2.3.2-15-2016-00035, GINOP-2.3.2-15-2016-00001, EFOP-3.6.1-16-2016-00008, NF101001, GINOP 2.3.2-15-2016-00034]; Hungarian Academy of Sciences [LP2017-7/2017] Funding text: This work was funded by National Research, Development and Innovation Office of Hungary (http://nkfih.gov.hu/) GINOP-2.3.2-15-2016-00032 to GJ, 2.3.2-15-2016-00035, GINOP-2.3.2-15-2016-00001, EFOP-3.6.1-16-2016-00008, NF101001 to RS, and GINOP 2.3.2-15-2016-00034 and Hungarian Academy of Sciences Lendulet Program Grant LP2017-7/2017 to ZL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Összes idézések száma a WoS-ban: 0 Department of Genetics, University of Szeged, Szeged, Hungary Department of Anatomy, Eötvös Loránd University, Budapest, Hungary Institute of Biochemistry and MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :3 Export Date: 20 July 2020 Correspondence Address: Sinka, R.; Department of Genetics, University of SzegedHungary; email: rsinka@bio.u-szeged.hu Department of Genetics, University of Szeged, Szeged, Hungary Department of Anatomy, Eötvös Loránd University, Budapest, Hungary Institute of Biochemistry and MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :6 Export Date: 13 January 2021 Correspondence Address: Sinka, R.; Department of Genetics, University of SzegedHungary; email: rsinka@bio.u-szeged.hu AB - Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide structural rigidity for flagellar movement, but its precise function and organization is incompletely understood. The two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The molecular constituents and precise function of the paracrystalline material have not yet been revealed. Here we purified the paracrystalline material from mature sperm and identified by mass spectrometry Sperm-Leucylaminopeptidase (S-Lap) family members as important constituents of it. To study the function of S-Lap proteins we show the characterization of classical mutants and RNAi lines affecting of the S-Lap genes and the analysis of their mutant phenotypes. We show that the male sterile phenotype of the S-Lap mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives. Our work shows that S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochondrial derivative. Therefore, we propose that S-Lap proteins are important constituents of the paracrystalline material of Drosophila melanogaster sperm. LA - English DB - MTMT ER - TY - JOUR AU - Vedelek, Viktor AU - Bodai, László AU - Grézal, Gábor AU - Kovács, Bence AU - Boros, Imre Miklós AU - Laurinyecz, Barbara AU - Sinka, Rita TI - Analysis of Drosophila melanogaster testis transcriptome JF - BMC GENOMICS J2 - BMC GENOMICS VL - 19 PY - 2018 IS - 1 PG - 19 SN - 1471-2164 DO - 10.1186/s12864-018-5085-z UR - https://m2.mtmt.hu/api/publication/30388331 ID - 30388331 LA - English DB - MTMT ER - TY - CONF AU - Jankovics, F AU - Bence, M AU - Takacs, Z AU - Laurinyecz, Barbara AU - Sinka, Rita AU - Erdelyi, M TI - Analysis of a novel gene required for ovarian germline stem cell maintenance and differentiation T2 - 25th European Drosophila Research Conference PY - 2017 SP - 314 PG - 1 UR - https://m2.mtmt.hu/api/publication/3273186 ID - 3273186 LA - English DB - MTMT ER - TY - CONF AU - Vedelek, Viktor AU - Laurinyecz, Barbara AU - Kovács, AL AU - Juhász, G AU - Sinka, Rita TI - Changes of mitochondria in Drosophila melanogaster spermatid T2 - 25th European Drosophila Research Conference PY - 2017 SP - 231 PG - 1 UR - https://m2.mtmt.hu/api/publication/3273183 ID - 3273183 LA - English DB - MTMT ER - TY - CONF AU - Boros, I AU - Sinka, Rita AU - Vedelek, Viktor AU - Grezal, G AU - Laurinyecz, Barbara AU - Kovacs, B AU - Bodai, László TI - Stage-specific transcriptome analysis in Drosophila testis T2 - 25th European Drosophila Research Conference PY - 2017 SP - 221 PG - 1 UR - https://m2.mtmt.hu/api/publication/3273127 ID - 3273127 LA - English DB - MTMT ER - TY - CHAP AU - Vedelek, Viktor AU - Grézal, Gábor AU - Laurinyecz, Barbara AU - Bence, Kovács AU - Boros, Imre Miklós AU - Bodai, László AU - Sinka, Rita ED - Zsuzsanna, Heiszler ED - Róbert, Hohol ED - Nóra, Éles-Etele TI - Stage-specific transcriptome analysis in Drosophila testis T2 - Hungarian Molecular Life Sciences 2017 PB - Diamond Congress Kft. CY - Budapest SN - 9786155270345 PY - 2017 SP - 233 EP - 233 PG - 1 UR - https://m2.mtmt.hu/api/publication/3208412 ID - 3208412 LA - English DB - MTMT ER - TY - CHAP AU - Laurinyecz, Barbara AU - Slezák, Csilla AU - Vedelek, Viktor AU - Szilasi, Kinga AU - Attila, Lajos Kovács AU - Gábor, Juhász AU - Zsuzsanna, Darula AU - Katalin, F Medzihradszky AU - Sinka, Rita ED - Zsuzsanna, Heiszler ED - Róbert, Hohol ED - Nóra, Éles-Etele TI - The most abundantly occurring protein family in the Drosophila sperm T2 - Hungarian Molecular Life Sciences 2017 PB - Diamond Congress Kft. CY - Budapest SN - 9786155270345 PY - 2017 SP - 158 EP - 158 PG - 1 UR - https://m2.mtmt.hu/api/publication/3208410 ID - 3208410 LA - English DB - MTMT ER - TY - JOUR AU - Vedelek, Viktor AU - Grézal, Gábor AU - Laurinyecz, Barbara AU - Bence, Kovács AU - Imre, Boros AU - Bodai, László AU - Sinka, Rita TI - Stage-specific transcriptome analysis in Drosophila testis JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 40 PY - 2016 IS - 3 SP - 67 EP - 67 PG - 1 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/3105635 ID - 3105635 LA - English DB - MTMT ER - TY - JOUR AU - Laurinyecz, Barbara AU - Slezák, Csilla AU - Vedelek, Viktor AU - Attila, Lajos Kovács AU - Gábor, Juhász AU - Sinka, Rita TI - Investigation the protein family occurring most abundantly in the Drosophila sperm JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 40 PY - 2016 IS - 3 SP - 54 EP - 54 PG - 1 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/3105628 ID - 3105628 LA - English DB - MTMT ER - TY - JOUR AU - Vedelek, Viktor AU - Laurinyecz, Barbara AU - Attila, L Kovács AU - Juhász, Gábor AU - Sinka, Rita TI - Testis-specific glutamate dehydrogenase is essential in the development of spermatid mitochondria JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 40 PY - 2016 IS - 3 SP - 38 EP - 38 PG - 1 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/3105627 ID - 3105627 LA - English DB - MTMT ER -