TY - JOUR AU - Blaskó, Ágnes AU - Gazdag, Zoltán AU - Gróf, Pál AU - Máté, Gábor AU - Tavaszi-Sárosi, Szilvia AU - Krisch, Judit AU - Vágvölgyi, Csaba AU - Makszin, Lilla AU - Pesti, Miklós TI - Effects of clary sage oil and its main components, linalool and linalyl acetate, on the plasma membrane of Candida albicans: an in vivo EPR study. JF - APOPTOSIS J2 - APOPTOSIS VL - 22 PY - 2017 IS - 2 SP - 175 EP - 187 PG - 13 SN - 1360-8185 DO - 10.1007/s10495-016-1321-7 UR - https://m2.mtmt.hu/api/publication/3137194 ID - 3137194 N1 - Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12, Pécs, 7624, Hungary Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Pécs, Hungary Institute of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Medical and Aromatic Plants, Faculty of Horticultural Sciences, Corvinus University of Budapest, Budapest, Hungary Institute of Food Engineering, Faculty of Engineering, University of Szeged, Szeged, Hungary Department of Microbiology, Faculty of Sciences and Informatics, University of Szeged, Szeged, Hungary Cited By :18 Export Date: 24 January 2024 CODEN: APOPF Correspondence Address: Blaskó, Á.; Institute of Bioanalysis, Szigeti út 12, Hungary; email: agnesblasko@hotmail.com AB - The effects of clary sage (Salvia sclarea L.) oil (CS-oil), and its two main components, linalool (Lol) and linalyl acetate (LA), on cells of the eukaryotic human pathogen yeast Candida albicans were studied. Dynamic and thermodynamic properties of the plasma membrane were investigated by electron paramagnetic resonance (EPR) spectroscopy, with 5-doxylstearic acid (5-SASL) and 16-SASL as spin labels. The monitoring of the head group regions with 5-SASL revealed break-point frequency decrease in a temperature dependent manner of the plasma membrane between 9.55 and 13.15 degrees C in untreated, in CS-oil-, Lol- and LA-treated membranes. The results suggest a significant increase in fluidity of the treated plasma membranes close to the head groups. Comparison of the results observed with the two spin labels demonstrated that CS-oil and LA induced an increased level of fluidization at both depths of the plasma membrane. Whereas Lol treatment induced a less (1 %) ordered bilayer organization in the superficial regions and an increased (10 %) order of the membrane leaflet in deeper layers. Acute toxicity tests and EPR results indicated that both the apoptotic and the effects exerted on the plasma membrane fluidity depended on the composition and chemical structure of the examined materials. In comparison with the control, treatment with CS-oil, Lol or LA induced 13.0, 12.3 and 26.4 % loss respectively, of the metabolites absorbing at 260 nm, as a biological consequence of the plasma membrane fluidizing effects. Our results confirmed that clary sage oil causes plasma membrane perturbations which leads to cell apoptosis process. LA - English DB - MTMT ER - TY - JOUR AU - Németh, Csilla Emese AU - Marcolongo, P AU - Gamberucci, A AU - Fulceri, R AU - Benedetti, A AU - Zoppi, N AU - Ritelli, M AU - Chiarelli, N AU - Colombi, M AU - Willaert, A AU - Callewaert, BL AU - Coucke, PJ AU - Gróf, Pál AU - Nagy, Szilvia Krisztina AU - Mészáros, Tamás AU - Bánhegyi, Gábor AU - Margittai, Éva TI - Glucose transporter type 10 - lacking in arterial tortuosity syndrome - facilitates dehydroascorbic acid transport JF - FEBS LETTERS J2 - FEBS LETT VL - 590 PY - 2016 IS - 11 SP - 1630 EP - 1640 PG - 11 SN - 0014-5793 DO - 10.1002/1873-3468.12204 UR - https://m2.mtmt.hu/api/publication/3075301 ID - 3075301 N1 - Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary Department of Molecular and Developmental Medicine, University of Siena, Italy Division of Biology and Genetics, Department of Molecular and Translational Medicine, Medical Faculty, University of Brescia, Italy Center for Medical Genetics, Ghent University, Belgium Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Institute of Clinical Experimental Research, Semmelweis University, Budapest, Hungary Cited By :9 Export Date: 3 September 2019 CODEN: FEBLA Correspondence Address: Margittai, É.; Institute of Clinical Experimental Research, Semmelweis UniversityHungary; email: margittai.eva@med.semmelweis-univ.hu Chemicals/CAS: dehydroascorbic acid, 33124-69-5, 490-83-5 Funding details: Hungarian Scientific Research Fund, G057413N Funding details: Universiteit Gent, BOF08/01M01108 Funding details: Fondazione Telethon, GGP13167 Funding details: Hungarian Scientific Research Fund Funding details: Magyar Tudományos Akadémia, MTA Funding details: Hungarian Scientific Research Fund, 111031, 100293, 112696, 105246 Funding text 1: This work was supported by the Hungarian Scientific Research Fund (OTKA), Grants 100293, 111031, 112696, 105246, by the Telethon Grant n. GGP13167, by Ghent University (Methusalem grant BOF08/01M01108) and by the Fund for Scientific Research ? Flanders (Research Project G057413N). ?.M. was supported by the J?nos Bolyai scholarship of the Hungarian Academy of Sciences, and by the E?tv?s Scholarship of the Hungarian State. AB - Loss-of-function mutations in the gene encoding GLUT10 are responsible for arterial tortuosity syndrome (ATS), a rare connective tissue disorder. In the present study GLUT10 mediated dehydroascorbic acid (DAA) transport was investigated, supposing its involvement in the pathomechanism. GLUT10 protein produced by in vitro translation and incorporated into liposomes efficiently transported DAA. Silencing of GLUT10 decreased DAA transport in immortalized human fibroblasts whose plasma membrane was selectively permeabilized. Similarly, the transport of DAA through endomembranes was markedly reduced in fibroblasts from ATS patients. Re-expression of GLUT10 in patients' fibroblasts restored DAA transport activity. The present results demonstrate that GLUT10 is a DAA transporter and DAA transport is diminished in the endomembranes of fibroblasts from ATS patients. This article is protected by copyright. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Szeiliné Türmer, Katalin AU - Orbán, József AU - Gróf, Pál AU - Nyitrai, Miklós TI - FASCIN and alpha-actinin can regulate the conformation of actin filaments JF - BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS J2 - BBA-GEN SUBJECTS VL - 1850 PY - 2015 IS - 9 SP - 1855 EP - 1861 PG - 7 SN - 0304-4165 DO - 10.1016/j.bbagen.2015.05.018 UR - https://m2.mtmt.hu/api/publication/2914852 ID - 2914852 N1 - Export Date: 27 January 2024; CODEN: BBGSB LA - English DB - MTMT ER - TY - JOUR AU - Bozó, Tamás AU - Brecska, R AU - Gróf, Pál AU - Kellermayer, Miklós TI - Extreme resilience in cochleate nanoparticles JF - LANGMUIR J2 - LANGMUIR VL - 31 PY - 2015 IS - 2 SP - 839 EP - 845 PG - 7 SN - 0743-7463 DO - 10.1021/la504428x UR - https://m2.mtmt.hu/api/publication/2802362 ID - 2802362 AB - Cochleates, prospective nanoscale drug delivery vehicles, are rolls of negatively-charged phospholipid membrane layers. The membrane layers are held together by calcium ions; however, neither the magnitude of membrane-interaction forces, nor the overall mechanical properties of cochleates have been known. Here we manipulated individual nanoparticles with atomic force microscopy to characterize their nanomechanical behavior. Their stiffness (4.2-12.5 N/m) and membrane-rupture forces (45.3-278 nN) are orders magnitude greater than those of the tough viral nanoshells. Even though the fundamental building material of cochleates is a fluid membrane, the combination of supramolecular geometry, the cross-linking action of calcium and the tight packing of the ions apparently lead to extreme mechanical resilience. The supramolecular design of cochleates may provide efficient protection for encapsulated materials and give clues to understanding biomolecular structures of similar design, such as the myelinated axon. LA - English DB - MTMT ER - TY - CONF AU - Budai, Lívia AU - Budai, Marianna AU - Gróf, Pál AU - Nóra, Mike-Kaszás AU - Pápay, Zsófia Edit AU - Füredi, Petra AU - Noémi, Anna Niczinger AU - Antal, István TI - Liposomesin pharmaceutical dosage forms and in cosmetics T2 - 3rd International Conference on Nutraceutical and Cosmetic Sciences PY - 2014 SP - 24 UR - https://m2.mtmt.hu/api/publication/2794545 ID - 2794545 LA - English DB - MTMT ER - TY - CONF AU - Bozó, Tamás AU - Brecska, R AU - Gróf, Pál AU - Kellerrmayer, MSZ TI - Membrán tekercsek morfológiája és mechanikai tulajdonságai T2 - 44. Membrán-transzport konferencia PB - Romhányi György Alapítvány C1 - Sümeg PY - 2014 SP - 35 UR - https://m2.mtmt.hu/api/publication/2739141 ID - 2739141 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Bozó, Tamás AU - Brecska, Richárd AU - Gróf, Pál AU - Kellermayer, Miklós TI - Kohleát rendszerek előállítása, morfológiája és nanomechanikája JF - GYÓGYSZERÉSZET J2 - GYÓGYSZERÉSZET VL - 86 PY - 2014 IS - Suppl. 1 SP - S104 SN - 0017-6036 UR - https://m2.mtmt.hu/api/publication/2578434 ID - 2578434 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Székács, Inna AU - Mike-Kaszás, Nóra AU - Gróf, Pál AU - Erdelyi, K AU - Szendro, I AU - Mihalik, Balázs AU - Pataki, A AU - Antoni, Ferenc András AU - Madarász, Emilia TI - Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities. JF - PLOS ONE J2 - PLOS ONE VL - 8 PY - 2013 IS - 12 PG - 11 SN - 1932-6203 DO - 10.1371/journal.pone.0081398 UR - https://m2.mtmt.hu/api/publication/2506863 ID - 2506863 AB - Optical waveguide lightmode spectroscopic (OWLS) techniques were probed for monitoring ion permeation through channels incorporated into artificial lipid environment. A novel sensor set-up was developed by depositing liposomes or cell-derived membrane fragments onto hydrophilic polytetrafluoroethylene (PTFE) membrane. The fibrous material of PTFE membrane could entrap lipoid vesicles and the water-filled pores provided environment for the hydrophilic domains of lipid-embedded proteins. The sensor surface was kept clean from the lipid holder PTFE membrane by a water- and ion-permeable polyethylene terephthalate (PET) mesh. The sensor set-up was tested with egg yolk lecithin liposomes containing gramicidin ion channels and with cell-derived membrane fragments enriched in GABA-gated anion channels. The method allowed monitoring the move of Na(+) and organic cations through gramicidin channels and detecting the Cl(-)-channel functions of the (alpha5beta2gamma2) GABAA receptor in the presence or absence of GABA and the competitive GABA-blocker bicuculline. LA - English DB - MTMT ER - TY - JOUR AU - Budai, Lívia AU - Mike-Kaszás, Nóra AU - Gróf, Pál AU - Földvári-Nagy Lászlóné Lenti, Katalin AU - Maghami, Katayoon AU - Antal, István AU - Klebovich, Imre AU - Petrikovics, Ilona AU - Budai, Marianna TI - Liposomes for topical use: physico-chemical comparison of vesicles prepared from egg or soy lecithin JF - SCIENTIA PHARMACEUTICA J2 - SCI PHARM VL - 81 PY - 2013 IS - 4 SP - 1151 EP - 1166 PG - 16 SN - 0036-8709 DO - 10.3797/scipharm.1305-11 UR - https://m2.mtmt.hu/api/publication/2461806 ID - 2461806 LA - English DB - MTMT ER - TY - JOUR AU - Kupi, Tünde AU - Gróf, Pál AU - Nyitrai, Miklós AU - Belágyi, József TI - Interaction of formin FH2 with skeletal muscle actin. EPR and DSC studies JF - EUROPEAN BIOPHYSICS JOURNAL J2 - EUR BIOPHYS J VL - 42 PY - 2013 IS - 10 SP - 757 EP - 765 PG - 9 SN - 0175-7571 DO - 10.1007/s00249-013-0922-0 UR - https://m2.mtmt.hu/api/publication/2443105 ID - 2443105 N1 - Cited By :5 Export Date: 17 October 2023 CODEN: EBJOE Correspondence Address: Nyitrai, M.; Department of Biophysics, Szigeti str. 12, Pécs 7624, Hungary; email: Miklos.nyitrai@aok.pte.hu LA - English DB - MTMT ER -