TY - JOUR AU - Mucsi, Márton AU - Kériné Borsodi, Andrea AU - Megyes, Melinda AU - Szili Kovács, Tibor TI - Response of the metabolic activity and taxonomic composition of bacterial communities to mosaically varying soil salinity and alkalinity JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-024-57430-2 UR - https://m2.mtmt.hu/api/publication/34768902 ID - 34768902 AB - Soil salinity and sodicity is a worldwide problem that affects the composition and activity of bacterial communities and results from elevated salt and sodium contents. Depending on the degree of environmental pressure and the combined effect of other factors, haloalkalitolerant and haloalkaliphilic bacterial communities will be selected. These bacteria play a potential role in the maintenance and restoration of salt-affected soils; however, until recently, only a limited number of studies have simultaneously studied the bacterial diversity and activity of saline–sodic soils. Soil samples were collected to analyse and compare the taxonomic composition and metabolic activity of bacteria from four distinct natural plant communities at three soil depths corresponding to a salinity‒sodicity gradient. Bacterial diversity was detected using 16S rRNA gene Illumina MiSeq amplicon sequencing. Community-level physiological profiles (CLPPs) were analysed using the MicroResp™ method. The genus-level bacterial composition and CLPPs differed significantly in soils with different alkaline vegetation. The surface soil samples also significantly differed from the intermediate and deep soil samples. The results showed that the pH, salt content, and Na + content of the soils were the main edaphic factors influencing both bacterial diversity and activity. With salinity and pH, the proportion of the phylum Gemmatimonadota increased, while the proportions of Actinobacteriota and Acidobacteriota decreased. LA - English DB - MTMT ER - TY - CHAP AU - Nagy, Balázs AU - Pereira, SR AU - Kovács, József AU - Kériné Borsodi, Andrea AU - Kern, Zoltán AU - Mihajlik, Gábor AU - Ignéczi, Ádám ED - Daniels, JA TI - Exploring the highest Atacama: Where the desert meets the tundra-water-related processes in the cold-dry environment T2 - Advances in Environmental Research. Volume 98 PB - Nova Science Publishers CY - Hauppauge, New York SN - 9798891134317 PY - 2024 SP - 35 EP - 68 PG - 34 UR - https://m2.mtmt.hu/api/publication/34599627 ID - 34599627 LA - English DB - MTMT ER - TY - JOUR AU - Lange-Enyedi, Nóra AU - Németh, Péter AU - Kériné Borsodi, Andrea AU - Spötl, C AU - Makk, Judit TI - Calcium carbonate precipitating extremophilic bacteria in an Alpine ice cave JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-024-53131-y UR - https://m2.mtmt.hu/api/publication/34560277 ID - 34560277 AB - Extensive research has provided a wealth of data on prokaryotes in caves and their role in biogeochemical cycles. Ice caves in carbonate rocks, however, remain enigmatic environments with limited knowledge of their microbial taxonomic composition. In this study, bacterial and archaeal communities of the Obstans Ice Cave (Carnic Alps, Southern Austria) were analyzed by next-generation amplicon sequencing and by cultivation of bacterial strains at 10 °C and studying their metabolism. The most abundant bacterial taxa were uncultured Burkholderiaceae and Brevundimonas spp. in the drip water, Flavobacterium, Alkanindiges and Polaromonas spp. in the ice, Pseudonocardia, Blastocatella spp., uncultured Pyrinomonadaceae and Sphingomonadaceae in carbonate precipitates, and uncultured Gemmatimonadaceae and Longimicrobiaceae in clastic cave sediments. These taxa are psychrotolerant/psychrophilic and chemoorganotrophic bacteria. On a medium with Mg2+/Ca2+ = 1 at 21 °C and 10 °C, 65% and 35% of the cultivated strains precipitated carbonates, respectively. The first ~ 200 µm-size crystals appeared 2 and 6 weeks after the start of the cultivation experiments at 21 °C and 10 °C, respectively. The crystal structure of these microbially induced carbonate precipitates and their Mg-content are strongly influenced by the Mg2+/Ca2+ ratio of the culture medium. These results suggest that the high diversity of prokaryotic communities detected in cryogenic subsurface environments actively contributes to carbonate precipitation, despite living at the physical limit of the presence of liquid water. LA - English DB - MTMT ER - TY - GEN AU - Lennert, K. AU - Engloner, Attila AU - Kériné Borsodi, Andrea TI - A mikrobióta taxonómiai összetételének változása a Duna különböző víztesttípusaiban és élőhelyein PY - 2023 UR - https://m2.mtmt.hu/api/publication/34475071 ID - 34475071 AB - Előadás LA - Hungarian DB - MTMT ER - TY - GEN AU - Lennert, K. AU - Engloner, Attila AU - Kériné Borsodi, Andrea TI - Taxonomic diversity of planktonic and biofilm bacterial communities in different Danube water body types in Hungary PY - 2023 SP - 79 UR - https://m2.mtmt.hu/api/publication/34475023 ID - 34475023 AB - Poster LA - English DB - MTMT ER - TY - CONF AU - ÁBEL, CSONGOR NÉMETH AU - NÓRA, TÜNDE LANGE-ENYEDI AU - M Tóth, Erika AU - PÉTER, NÉMETH AU - IVETT, KOVÁCS AU - PÉTER, DOBOSY AU - ATTILA, DEMÉNY AU - Kériné Borsodi, Andrea AU - Makk, Judit ED - K., MÁRIALIGETI ED - O., DOBAY TI - Geomicrobiological study of modern microbialites in a thermal spring (Köröm, Hungary) T2 - Abstracts of the 19th International Congress of the Hungarian Society for Microbiology PY - 2023 SP - 74 UR - https://m2.mtmt.hu/api/publication/34474922 ID - 34474922 LA - English DB - MTMT ER - TY - CONF AU - Lennert, K. AU - Kériné Borsodi, Andrea AU - Engloner, A. ED - K., MÁRIALIGETI ED - O., DOBAY TI - Variation in the taxonomic composition of microbiota by type of water body and habitat in the river Danube T2 - Abstracts of the 19th International Congress of the Hungarian Society for Microbiology PY - 2023 SP - 70 UR - https://m2.mtmt.hu/api/publication/34474905 ID - 34474905 LA - English DB - MTMT ER - TY - JOUR AU - Ács, Éva AU - Kériné Borsodi, Andrea AU - Kiss, Keve Tihamér AU - Teszárné, Nagy Mariann TI - Reskóné Dr. Nagy Mária – Ács Éva, Borsodi Andrea, Kiss Keve Tihamér és Teszárné Nagy Mariann megemlékezése JF - HIDROLÓGIAI KÖZLÖNY J2 - HIDROL KOZL VL - 103 PY - 2023 IS - 4 SP - 80 EP - 80 PG - 1 SN - 0018-1323 UR - https://m2.mtmt.hu/api/publication/34431758 ID - 34431758 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szili Kovács, Tibor AU - Mucsi, Márton AU - Füzy, Anna AU - Takács, Tünde Mária AU - Kériné Borsodi, Andrea TI - A talaj katabolikus aktivitás mintázatának elemzése mikrorespirációs (MicroResp™) módszerrel JF - AGROKÉMIA ÉS TALAJTAN J2 - AGROKÉMIA ÉS TALAJTAN VL - 72 PY - 2023 IS - 2 SP - 118 EP - 131 PG - 14 SN - 0002-1873 DO - 10.1556/0088.2023.00153 UR - https://m2.mtmt.hu/api/publication/34431713 ID - 34431713 AB - A talaj mikrobiális közösségének funkcionális diverzitása a talaj ökoszisztéma szolgáltatások jelentős részéhez hozzájárul, sok esetben meghatározó jelentőségű. Többféle kísérleti és elméleti megközelítés közül a katabolikus aktivitás-mintázat mikrorespirációs – MicroResp™ – módszerrel történő megközelítését mutatjuk be. A módszer a régebbről ismert szubsztrát-indukált respiráció több-szubsztrátos, mikrotiter lemez alapú kiterjesztése, amivel a talaj mikroba-közösség in-situ közösségi-szintű fiziológiai mintázata határozható meg. Mivel az egyes mikroorganizmusok szubsztrát-hasznosítása eltérő, a mikroba-közösség aktuális összetételétől, abundanciájától függően változó a szubsztrát hasznosítási mintázat egy-egy talajminta esetében. Az alkalmazott szubsztrátok köre tetszőleges, rendszerint egyszerű cukrok, aminosavak, aminok, karbonsavak. A módszer gyors, érzékeny, megbízható, ezért alkalmazása tervezett kísérletekben és talajmonitoring programokban egyaránt javasolható. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Lange-Enyedi, Nóra AU - Kériné Borsodi, Andrea AU - Németh, Péter AU - Czuppon, György AU - Kovács, Ivett AU - Leél-Őssy, Szabolcs AU - Dobosy, Péter AU - Felföldi, Tamás AU - Demény, Attila AU - Makk, Judit TI - Habitat-related variability in the morphological and taxonomic diversity of microbial communities in two Hungarian epigenic karst caves JF - FEMS MICROBIOLOGY ECOLOGY J2 - FEMS MICROBIOL ECOL VL - 99 PY - 2023 IS - 12 PG - 17 SN - 0168-6496 DO - 10.1093/femsec/fiad161 UR - https://m2.mtmt.hu/api/publication/34431117 ID - 34431117 N1 - Institute for Geological and Geochemical Research, HUN-REN Research Centre for Astronomy and Earth Sciences, Budaörsi út 45, Budapest, H-1112, Hungary Department of Microbiology, Institute of Biology, Faculty of Science, Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, H-1117, Hungary Institute of Aquatic Ecology, HUN-REN Centre for Ecological Research, Karolina út 29, Budapest, H-1113, Hungary Research Institute of Biomolecular and Chemical Engineering, Nanolab, University of Pannonia, Egyetem út 10, Veszprém, H-8200, Hungary Department of Physical and Applied Geology, Faculty of Science, Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, H-1117, Hungary Export Date: 8 February 2024 CODEN: FMECE Correspondence Address: Makk, J.; Department of Microbiology, Pázmány P. sétány 1/C, Hungary; email: makk.judit@ttk.elte.hu AB - The physical and chemical characteristics of the bedrock, along with the geological and hydrological conditions of karst caves may influence the taxonomic and functional diversity of prokaryotes. Most studies so far have focused on microbial communities of caves including only a few samples and have ignored the chemical heterogeneity of different habitat types such as sampling sites, dripping water, carbonate precipitates, cave walls, cave sediment and surface soils connected to the caves. The aim of the present study was to compare the morphology, the composition and physiology of the microbiota in caves with similar environmental parameters (temperature, host rock, elemental and mineral composition of speleothems) but located in different epigenic karst systems. Csodabogyós Cave and Baradla Cave (Hungary) were selected for the analysis of bacterial and archaeal communities using electron microscopy, amplicon sequencing, X-ray diffraction and mass spectroscopic techniques. The microbial communities belonged to the phyla Pseudomonadota, Acidobacteriota, Nitrospirota and Nitrososphaerota, and they showed site-specific variation in composition and diversity. The results indicate that morphological and physiological adaptations provide survival for microorganisms according to the environment. In epigenic karst caves, prokaryotes are prone to increase their adsorption surface, cooperate in biofilms, and implement chemolithoautotrophic growth with different electron-donors and acceptors available in the microhabitats. LA - English DB - MTMT ER -