TY - JOUR AU - Balló, András AU - Czétány, Péter AU - Székvári, Kinga AU - Márk, László AU - Henn-Mike, Nóra AU - Török, Attila AU - Szántó, Árpád László AU - Máté, Gábor TI - Oxido-Reduction Potential as a Method to Determine Oxidative Stress in Semen Samples JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 15 PG - 17 SN - 1661-6596 DO - 10.3390/ijms241511981 UR - https://m2.mtmt.hu/api/publication/34106696 ID - 34106696 N1 - ISSN:1422-0067 AB - There are different estimates for the incidence of infertility. Its occurrence may vary from area to area, but on average, it affects 15% of couples and 10-12% of men worldwide. Many aspects of infertility can be linked to reactive oxygen species (ROS) and the process of oxidative stress (OS). The association between poor semen quality and OS is well known. Unfortunately, there is no accepted protocol for the diagnosis and treatment of OS in andrology. Oxido-reduction potential (ORP) measurement is a new method for determining the ratio between oxidant and antioxidant molecules. Currently, ORP measurement is one of the fastest and most user-friendly methods of andrological OS determination and our goals were to confirm published correlations between ORP values and sperm parameters, examine how sperm concentration influences these results, and investigate whether intracellular ROS formations are also manifested in the ORP values or not after artificial ROS induction. Intracellular ROS formations were induced by menadione (superoxide anion inducer), hydrogen peroxide, and tert-butyl hydroperoxide (lipid peroxidation inducer) treatments; sperm parameters like motility and viability were determined with an SCA Scope system, and ORP changes were recorded by the Mioxsys system. Significant correlations were noticed among the ORP, spermatozoa concentration, motility, progressive motility, and viability. Nevertheless, only the ORP value after normalization with the sperm count correlated with these parameters. Due to normalization, very low and very high sperm concentrations can give misleading results. The means of the non-normalized ORP values were almost the same. All of the applied treatments resulted in decreases in the viability, motility, and progressive motility, and interestingly, altered ORP levels were detected. In addition, it was determined that seminal plasma had a significant protective effect on spermatozoa. The elimination of seminal plasma caused higher sensitivity of spermatozoa against used OS inducers, and higher ORP levels and decreased viabilities and motilities were measured. The ORP level could be a good indicator of male OS; however, in cases of low and high sperm counts, its result can be misleading. Overall, the conclusion can be drawn that ORP determination is a suitable method for detecting intracellular ROS accumulation, but it has limitations that still need to be clarified. LA - English DB - MTMT ER - TY - JOUR AU - Balló, András AU - Székvári, Kinga AU - Czétány, Péter AU - Márk, László AU - Török, Attila AU - Szántó, Árpád László AU - Máté, Gábor TI - Estrogenic and Non-Estrogenic Disruptor Effect of Zearalenone on Male Reproduction : A Review JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 2 PG - 24 SN - 1661-6596 DO - 10.3390/ijms24021578 UR - https://m2.mtmt.hu/api/publication/33585064 ID - 33585064 N1 - Export Date: 18 January 2024 AB - According to some estimates, at least 70% of feedstuffs and finished feeds are contaminated with one or more mycotoxins and, due to its significant prevalence, both animals and humans are highly likely to be exposed to these toxins. In addition to health risks, they also cause economic issues. From a healthcare point of view, zearalenone (ZEA) and its derivatives have been shown to exert many negative effects. Specifically, ZEA has hepatotoxicity, immunotoxicity, genotoxicity, carcinogenicity, intestinal toxicity, reproductive toxicity and endocrine disruption effects. Of these effects, male reproductive deterioration and processes that lead to this have been reviewed in this study. Papers are reviewed that demonstrate estrogenic effects of ZEA due to its analogy to estradiol and how these effects may influence male reproductive cells such as spermatozoa, Sertoli cells and Leydig cells. Data that employ epigenetic effects of ZEA are also discussed. We discuss literature data demonstrating that reactive oxygen species formation in ZEA-exposed cells plays a crucial role in diminished spermatogenesis; reduced sperm motility, viability and mitochondrial membrane potential; altered intracellular antioxidant enzyme activities; and increased rates of apoptosis and DNA fragmentation; thereby resulting in reduced pregnancy. LA - English DB - MTMT ER - TY - JOUR AU - Máté, Gábor AU - Balló, András AU - Márk, László AU - Czétány, Péter AU - Szántó, Árpád László AU - Török, Attila TI - Magnetic-Activated Cell Sorting as a Method to Improve Necrozoospermia-Related Asthenozoospermic Samples. JF - JOURNAL OF CLINICAL MEDICINE J2 - J CLIN MED VL - 11 PY - 2022 IS - 10 PG - 7 SN - 2077-0383 DO - 10.3390/jcm11102914 UR - https://m2.mtmt.hu/api/publication/32849802 ID - 32849802 AB - According to some statistics, absolute asthenozoospermia affects every 1 in 5000 men. Although this incidence rate does not appear to be too high, it is extremely important to address the phenomenon because it can drastically reduce the chances of pregnancy, even with assisted reproduction. The biggest problem with absolute asthenozoospermia is that it is difficult to distinguish between live and dead sperm cells, and fertilization with non-viable spermatozoa may contribute to the failure of an assisted reproduction cycle. Nowadays, DNA fragmentation (DF) is a crucial parameter of semen analysis, and in this paper, we provide evidence of the correlation between DF and vitality. For this purpose, the main semen parameters were investigated by a CASA system (concentration, motility, progressive motility, vitality and DF). In the necrozoospermic group (vitality < 58%), all the measured parameters showed significant differences compared to normal vitality. Concentration (30.1 M mL-1 vs. 13.6 M mL-1), motility (31.9% vs. 18.3%), and progressive motility (24.3% vs. 12.7%) were significantly decreased, while DF was significantly increased (17.4% vs. 23.7%). Based on the connection between vitality decrement and DF increment, DF lowering methods, such as magnetic-activated cell sorting, have been hypothesized as novel methods for the elimination of dead spermatozoa. LA - English DB - MTMT ER - TY - JOUR AU - Török, Attila AU - Máté, Gábor TI - Endometriosis and Oxidative Stress. Do Reactive Oxygen Species Always Lead to Damages of Gametes and Embryos? JF - AUSTIN JOURNAL OF REPRODUCTIVE MEDICINE & INFERTILITY J2 - AUSTIN JOURNAL OF REPRODUCTIVE MEDICINE & INFERTILITY VL - 7 PY - 2021 IS - 1 SP - 1054 SN - 2471-0393 UR - https://m2.mtmt.hu/api/publication/32096436 ID - 32096436 AB - Reactive Oxygen Species (ROS) play a crucial role in the pathogenesis of many reproductive disorders, such as endometriosis on the one hand, but on the other hand they participate in different cellular proliferation processes, too. Endometriosis is an apoptotic endometrial, menstrual cells and lysed erythrocytes-induced inflammatory disease outside the uterine cavity, which activates macrophages leading to ROS production and oxidative stress. However, based on the available literature, the reproductive outcomes are still contradictory. In this study, the demographic, embryological and clinical results of 252 patients suffering from tubal infertility (control), ASRM I-II and III-IV endometriosis were analyzed. Endometriosis was associated with decreased anti-Müllerian hormone level and increased gonadotropin doses during stimulation (p<0.0001). In ASRM III-IV, reduced embryological parameters were observed, which resulted in 13.73% and 15.21% decrements in the implantation rates, 19.96% and 23.89% in the clinical pregnancy rates of patients suffering from ASRM III-IV endometriosis in comparison with control or ASRM I-II, respectively. In addition, miscarriage rates were 19.04%, 29.03% and 38.46% in control, ASRM I-II and ASRM III-IV, respectively. In our study, the supposed altered oxido-reduction environment of gametes and embryos obviously exerted negative effects on the embryological and clinical parameters, but these effects could not be observed in case of mild endometriosis with low level of stress. LA - English DB - MTMT ER - TY - JOUR AU - Máté, Gábor AU - Török, Attila TI - Spermiumdiagnosztikai- és szelekciós eljárások JF - MAGYAR NŐORVOSOK LAPJA J2 - MAGYAR NŐORVOSOK LAPJA VL - 83 PY - 2020 IS - 5 SP - 271 EP - 275 PG - 5 SN - 0025-021X UR - https://m2.mtmt.hu/api/publication/31789565 ID - 31789565 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Máté, Gábor AU - Török, Attila TI - Elimination of stress factors by continuous embryo culture and its influence on in vitro fertilization outcomes JF - REPRODUCTIVE BIOLOGY J2 - REPROD BIOL VL - 20 PY - 2020 IS - 4 SP - 512 EP - 519 PG - 8 SN - 1642-431X DO - 10.1016/j.repbio.2020.08.004 UR - https://m2.mtmt.hu/api/publication/31789560 ID - 31789560 LA - English DB - MTMT ER - TY - CHAP AU - Török, Attila AU - Szmatona, Gábor ED - Steven, G Kaáli ED - Bernard, Artúr ED - Kovács, Péter Tamás TI - Az in vitro fertilizáció és emnrió transzfer menete T2 - A meddőség korszerű diagnosztikája és kezelése PB - Medicina Könyvkiadó CY - Budapest SN - 9632260422 PY - 2006 SP - 167133 EP - 138 UR - https://m2.mtmt.hu/api/publication/2153301 ID - 2153301 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Török, Attila ED - Steven, G Kaáli ED - Bernard, Artúr ED - Kovács, Péter Tamás TI - Habituális abortusz T2 - A meddőség korszerű diagnosztikája és kezelése PB - Medicina Könyvkiadó CY - Budapest SN - 9632260422 PY - 2006 SP - 231 EP - 248 PG - 18 UR - https://m2.mtmt.hu/api/publication/2153296 ID - 2153296 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Garai, János AU - Molnár, Valéria AU - Varga, T AU - Koppán, Miklós AU - Török, Attila AU - Bódis, József TI - Endometriosis: harmful survival of an ectopic tissue JF - FRONTIERS IN BIOSCIENCE J2 - FRONT BIOSCI VL - 11 PY - 2006 SP - 595 EP - 619 PG - 25 SN - 1093-9946 DO - 10.2741/1821 UR - https://m2.mtmt.hu/api/publication/1176928 ID - 1176928 N1 - Export Date: 27 January 2024 LA - English DB - MTMT ER - TY - JOUR AU - Garai, János AU - Molnár, Valéria AU - Varga, T AU - Török, Attila AU - Koppán, Miklós AU - Bódis, József TI - Macrophage migration inhibitory factor (MIF). Enzymatic activity of peritoneal fluids in endometriosis JF - FEBS JOURNAL J2 - FEBS J VL - 272 PY - 2005 IS - Suppl. 1 SP - 289 EP - 290 PG - 2 SN - 1742-464X UR - https://m2.mtmt.hu/api/publication/1431030 ID - 1431030 LA - English DB - MTMT ER -