@article{MTMT:34836449, title = {Antibacterial activity of Hungarian varietal honeys against respiratory pathogens as a function of storage time}, url = {https://m2.mtmt.hu/api/publication/34836449}, author = {Radványi, Lilla and Balázs, Viktória Lilla and Kocsis, Béla and Csikós, Eszter and Ángyán, Virág Diána and Szabó, Péter and Biró, Viktória and Kocsis, Marianna and Farkas, Ágnes}, doi = {10.1038/s41598-024-60961-3}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34836449}, issn = {2045-2322}, abstract = {Today, antibiotic therapies that previously worked well against certain bacteria due to their natural sensitivity, are becoming less effective. Honey has been proven to inhibit the biofilm formation of some respiratory bacteria, however few data are available on how the storage time affects the antibacterial effect. The activity of black locust, goldenrod, linden and sunflower honeys from three consecutive years (2020, 2021, 2022) was analyzed in 2022 against Gram-negative (Haemophilus influenzae, H. parainfluenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) bacteria using in vitro microbiological methods. After determining the physicochemical parameters of honey, broth microdilution was applied to determine the minimum inhibitory concentration of each honey type against each bacterium, and crystal violet assay was used to test their antibiofilm effect. The possible mechanism of action was explored with membrane degradation test, while structural changes were illustrated with scanning electron microscopy. Honeys stored for one or two years were darker than fresh honeys, while older honeys had significantly lower antibacterial activity. The most remarkable inhibitory effect was exerted by linden and sunflower honeys, and P. aeruginosa proved to be the most resistant bacterium. Based on our results, honey intended for medicinal purposes should be used as fresh as possible during a treatment.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Csikós, Eszter/0000-0002-5368-9492; Farkas, Ágnes/0000-0002-8590-3203} } @CONFERENCE{MTMT:34826025, title = {Potential new treatment target in bacterial keratitis}, url = {https://m2.mtmt.hu/api/publication/34826025}, author = {Mérész, Balázs and Inez, Bosnyák and Molitor, Dorottya and Kocsis, Béla and Borbásné Farkas, Kornélia and Reglődi, Dóra and Atlasz, Tamás and Váczy, Alexandra}, booktitle = {Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference}, unique-id = {34826025}, year = {2024}, pages = {154-154}, orcid-numbers = {Borbásné Farkas, Kornélia/0000-0002-5349-6527; Atlasz, Tamás/0000-0002-8112-8633} } @CONFERENCE{MTMT:34814487, title = {A gesztenyeméz biofilm képződést gátló hatása}, url = {https://m2.mtmt.hu/api/publication/34814487}, author = {Koloh, Regina and Farkas, Ágnes and Balázs, Viktória Lilla and Kocsis, Béla}, booktitle = {Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference}, unique-id = {34814487}, year = {2024}, pages = {87-87}, orcid-numbers = {Farkas, Ágnes/0000-0002-8590-3203} } @CONFERENCE{MTMT:34814458, title = {Mi az evidenciaalapú megközelítés alapján megítélt összefüggés a Pseudomonas aeruginosa baktérium és a kakukkfű illóolaj között?}, url = {https://m2.mtmt.hu/api/publication/34814458}, author = {Nagy, Anett and Ormai, Edit and Horváth, Györgyi and Kocsis, Béla and Balázs, Viktória Lilla}, booktitle = {Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference}, unique-id = {34814458}, year = {2024}, pages = {86-86}, orcid-numbers = {Horváth, Györgyi/0000-0001-5344-0294} } @CONFERENCE{MTMT:34814440, title = {Hársméz, levendula illóolaj és kombinációjuk antibakteriális aktivitása}, url = {https://m2.mtmt.hu/api/publication/34814440}, author = {Ángyán, Virág Diána and Farkas, Ágnes and Kocsis, Béla and Radványi, Lilla}, booktitle = {Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference}, unique-id = {34814440}, year = {2024}, pages = {85-85}, orcid-numbers = {Farkas, Ágnes/0000-0002-8590-3203} } @CONFERENCE{MTMT:34814410, title = {Nozokomiális patogének és a szegfűszeg illóolaj kapcsolatának bemutatása evidenciaalapú megközelítésben}, url = {https://m2.mtmt.hu/api/publication/34814410}, author = {Ormai, Edit and Kocsis, Béla and Böszörményi, Andrea and Horváth, Györgyi and Balázs, Viktória Lilla}, booktitle = {Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference}, unique-id = {34814410}, year = {2024}, pages = {83-83}, orcid-numbers = {Horváth, Györgyi/0000-0001-5344-0294} } @CONFERENCE{MTMT:34795108, title = {Négy gyógyszerkönyvi illóolaj antibakteriális hatásának vizsgálata a Pseudomonas aeruginosa baktériummal szemben}, url = {https://m2.mtmt.hu/api/publication/34795108}, author = {Nagy, Anett and Ormai, Edit and Horváth, Györgyi and Kocsis, Béla and Balázs, Viktória Lilla}, booktitle = {Abstract Book of the 7th International Cholnoky Symposium}, unique-id = {34795108}, year = {2024}, pages = {23-23}, orcid-numbers = {Horváth, Györgyi/0000-0001-5344-0294} } @CONFERENCE{MTMT:34795054, title = {A gesztenyeméz biofilmgátló hatása az idő függvényében}, url = {https://m2.mtmt.hu/api/publication/34795054}, author = {Koloh, Regina and Farkas, Ágnes and Kocsis, Béla and Kocsis, Marianna and Balázs, Viktória Lilla}, booktitle = {Abstract Book of the 7th International Cholnoky Symposium}, unique-id = {34795054}, year = {2024}, pages = {21-21}, orcid-numbers = {Farkas, Ágnes/0000-0002-8590-3203} } @CONFERENCE{MTMT:34795038, title = {A borsmenta illóolaj hatása kórházi környezetben előforduló patogéneken}, url = {https://m2.mtmt.hu/api/publication/34795038}, author = {Ormai, Edit and Kocsis, Béla and Böszörményi, Andrea and Horváth, Györgyi and Balázs, Viktória Lilla}, booktitle = {Abstract Book of the 7th International Cholnoky Symposium}, unique-id = {34795038}, year = {2024}, pages = {19-19}, orcid-numbers = {Böszörményi, Andrea/0000-0003-3982-7059; Horváth, Györgyi/0000-0001-5344-0294} } @article{MTMT:34753258, title = {Gram-negative rough mutants used as test bacteria can increase sensitivity of direct bioautography}, url = {https://m2.mtmt.hu/api/publication/34753258}, author = {Balázs, Viktória Lilla and Böszörményi, Andrea and Kocsis, Béla and Horváth, Györgyi}, doi = {10.1007/s00764-024-00293-0}, journal-iso = {J PLANAR CHROMATOGR MOD TLC}, journal = {JPC - JOURNAL OF PLANAR CHROMATOGRAPHY - MODERN TLC}, unique-id = {34753258}, issn = {0933-4173}, abstract = {Currently, the antimicrobial activity of essential oils (EOs) is an outstanding research field due to antibiotic resistance of microorganisms. Thin-layer chromatography‒direct bioautography (TLC‒DB) is an effective, fast method to find components with antimicrobial activity in a mixture of plant compounds, e.g., in EOs. The volatility and hydrophobic characters of EOs require special experimental conditions, and disc diffusion assay is not appropriate to explore the antimicrobial activity of them. The aim of this study was to use “R” mutants, which are more sensitive to synthetic antimicrobial drugs, in DB to increase the sensitivity of this method. Our hypothesis was that these mutants show sensitivity to some EOs (thyme, clove, and peppermint) as well. The chemical composition of our tested EOs was measured with gas chromatography‒mass spectrometry (GC‒MS). The main compounds (39.8% thymol, 78.8% eugenol, and 50.4% menthol) of EOs showed notable antibacterial activity in TLC‒DB. Based on our results, we suggest to use Salmonella minnesota Re595 rough strain as test bacterium in bioautography, because it showed the highest sensitivity to the tested antibiotics (gentamicin and cephalexin) and EOs. Furthermore, this rough mutant could make TLC‒DB more faster, because only 4 h incubation time was enough to detect the inhibition zones of the active compounds used in this study.}, year = {2024}, eissn = {1789-0993}, orcid-numbers = {Böszörményi, Andrea/0000-0003-3982-7059; Horváth, Györgyi/0000-0001-5344-0294} }