@article{MTMT:34561992, title = {Tadpoles Develop Elevated Heat Tolerance in Urban Heat Islands Regardless of Sex}, url = {https://m2.mtmt.hu/api/publication/34561992}, author = {Bókony, Veronika and Balogh, Emese and Ujszegi, János and Ujhegyi, Nikolett and Szederkényi, Márk and Hettyey, Attila}, doi = {10.1007/s11692-024-09626-7}, journal-iso = {EVOL BIOL}, journal = {EVOLUTIONARY BIOLOGY}, unique-id = {34561992}, issn = {0071-3260}, abstract = {The ability of wildlife to endure the effects of high temperatures is increasingly important for biodiversity conservation under climate change and spreading urbanization. Organisms living in urban heat islands can have elevated heat tolerance via phenotypic or transgenerational plasticity or microevolution. However, the prevalence and mechanisms of such thermal adaptations are barely known in aquatic organisms. Furthermore, males and females can differ in heat tolerance, which may lead to sex-biased mortality, yet it is unknown how sex differences in thermal biology influence urban phenotypic divergence. To address these knowledge gaps, we measured critical thermal maxima (CTmax) in male and female agile frog (Rana dalmatina) tadpoles captured from warm urban ponds and cool woodland ponds, and in a common-garden experiment where embryos collected from both habitat types were raised in the laboratory. We found higher CTmax in urban-dwelling tadpoles compared to their counterparts living in woodland ponds. This difference was reversed in the common-garden experiment: tadpoles originating from urban ponds had lower CTmax than tadpoles originating from woodland ponds. We found no effect of sex on CTmax or its difference between habitats. These results demonstrate that aquatic amphibian larvae can respond to the urban heat island effect with increased heat tolerance similarly to other, mostly terrestrial taxa studied so far, and that phenotypic plasticity may be the main driver of this response. Our findings also suggest that heat-induced mortality may be independent of sex in tadpoles, but research is needed in many more taxa to explore potentially sex-dependent urban thermal responses.}, keywords = {sex difference; ANURANS; phenotypic plasticity; Urban heat island; THERMAL TOLERANCE}, year = {2024}, eissn = {1934-2845}, orcid-numbers = {Bókony, Veronika/0000-0002-2136-5346} } @article{MTMT:34740499, title = {Editorial}, url = {https://m2.mtmt.hu/api/publication/34740499}, author = {Hettyey, Attila and Páll-Gergely, Barna}, doi = {10.17109/AZH.69.3.197.2023}, journal-iso = {ACTA ZOOLOG ACAD SCI HUNG}, journal = {ACTA ZOOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE}, volume = {69}, unique-id = {34740499}, issn = {1217-8837}, year = {2023}, eissn = {2064-2474}, pages = {197-198}, orcid-numbers = {Páll-Gergely, Barna/0000-0002-6167-7221} } @{MTMT:34547623, title = {Egy glifozátalapú gyomirtó ökotoxikológiai hatásai a vízi szervezetekre}, url = {https://m2.mtmt.hu/api/publication/34547623}, author = {Mikó, Zsanett and Bókony, Veronika and Ujszegi, János and Hettyey, Attila}, booktitle = {Tudományos kutatások a környezetbarát növényvédelemért a HUN-REN ATK Növényvédelmi Intézetében}, unique-id = {34547623}, year = {2023}, pages = {8-11}, orcid-numbers = {Bókony, Veronika/0000-0002-2136-5346} } @article{MTMT:34506237, title = {A felülfertőződés ökológiája kétéltűeknél: Különböző paraziták, ha egyszerre támadnak}, url = {https://m2.mtmt.hu/api/publication/34506237}, author = {Hettyey, Attila and Kásler, Andrea and Ujszegi, János and Herczeg, Dávid}, journal-iso = {ÉLET ÉS TUDOMÁNY}, journal = {ÉLET ÉS TUDOMÁNY}, volume = {47}, unique-id = {34506237}, issn = {0013-6077}, year = {2023}, pages = {1484-1487}, orcid-numbers = {Herczeg, Dávid/0000-0001-5098-961X} } @article{MTMT:34111344, title = {Metabolites of Xenorhabdus bacteria are potent candidates for mitigating amphibian chytridiomycosis}, url = {https://m2.mtmt.hu/api/publication/34111344}, author = {Ujszegi, János and Boros, Zsófia and Fodor, András and Vajna, Balázs and Hettyey, Attila}, doi = {10.1186/s13568-023-01585-0}, journal-iso = {AMB EXPRESS}, journal = {AMB EXPRESS}, volume = {13}, unique-id = {34111344}, issn = {2191-0855}, abstract = {Chytridiomycosis, caused by the chytrid fungus Batrachochytrium dendrobatidis (Bd), has caused extreme losses in amphibian biodiversity. Finding bacteria that produce metabolites with antifungal properties may turn out to be invaluable in the fight against this devastating disease. The entomopathogenic bacteria, Xenorhabdus szentirmaii and X. budapestensis produce secondary metabolites that are effective against a wide range of fungal plant pathogens. To assess whether they may also be effective against Bd, we extracted cell-free culture media (CFCM) from liquid cultures of X. szentirmaii and X. budapestensis and tested their ability to inhibit Bd growth in vitro. As a second step, using juvenile common toads ( Bufo bufo ) experimentally infected with Bd we also tested the in vivo antifungal efficacy of X. szentirmaii CFCM diluted to 2 and 10% (v/v), while also assessing possible malign side effects on amphibians. Results of the in vitro experiment documented highly effective growth inhibition by CFCMs of both Xenorhabdus species. The in vivo experiment showed that treatment with CFCM of X. szentirmaii applied at a dilution of 10% resulted in infection intensities reduced by ca. 73% compared to controls and to juvenile toads treated with CFCM applied at a dilution of 2%. At the same time, we detected no negative side effects of treatment with CFCM on toad survival and development. Our results clearly support the idea that metabolites of X. szentirmaii , and perhaps of several other Xenorhabdus species as well, may prove highly useful for the treatment of Bd infected amphibians.}, year = {2023}, eissn = {2191-0855}, orcid-numbers = {Vajna, Balázs/0000-0002-5604-7997} } @article{MTMT:34074949, title = {Amphibian larvae benefit from a warm environment under simultaneous threat from chytridiomycosis and ranavirosis}, url = {https://m2.mtmt.hu/api/publication/34074949}, author = {Herczeg, Dávid and Holly, Dóra and Kásler, Andrea and Bókony, Veronika and Papp, Tibor and Takács-Vágó, Hunor and Ujszegi, János and Hettyey, Attila}, doi = {10.1111/oik.09953}, journal-iso = {OIKOS}, journal = {OIKOS}, volume = {11}, unique-id = {34074949}, issn = {0030-1299}, year = {2023}, eissn = {1600-0706}, orcid-numbers = {Herczeg, Dávid/0000-0001-5098-961X; Bókony, Veronika/0000-0002-2136-5346; Papp, Tibor/0000-0002-6402-3864} } @article{MTMT:34010493, title = {Does The Glucocorticoid Stress Response Make Toads More Toxic? An Experimental Study on The Regulation of Bufadienolide Toxin Synthesis}, url = {https://m2.mtmt.hu/api/publication/34010493}, author = {Üveges, Bálint and Kalina, C and Szabó, Krisztina and Móricz, Ágnes and Holly, Dóra and Gabor, C R and Hettyey, Attila and Bókony, Veronika}, doi = {10.1093/iob/obad021}, journal = {Integrative Organismal Biology}, volume = {5}, unique-id = {34010493}, abstract = {Chemical defence is a crucial component of fitness in many organisms, yet the physiological regulation of defensive toxin synthesis is poorly understood, especially in vertebrates. Bufadienolides, the main defensive compounds of toads, are toxic to many predators and other natural enemies, and their synthesis can be upregulated by stressors including predation risk, high conspecific density, and pollutants. Thus, higher toxin content may be the consequence of a general endocrine stress response in toads. Therefore, we hypothesized that bufadienolide synthesis may be stimulated by elevated levels of corticosterone (CORT), the main glucocorticoid hormone of amphibians, or by upstream regulators that stimulate CORT production. To test these alternatives, we treated common toad tadpoles with exogenous CORT (exoCORT) or metyrapone (MTP, a CORT-synthesis inhibitor that stimulates upstream regulators of CORT by negative feedback) in the presence or absence of predation cues for 2 or 6 days, and subsequently measured their CORT release rates and bufadienolide content. We found that CORT release rates were elevated by exoCORT, and to a lesser extent also by MTP, regardless of treatment length. Bufadienolide content was significantly decreased by treatment with exoCORT for 6 days, but was unaffected by exposure to exoCORT for 2 days or to MTP for either 6 or 2 days. Presence or absence of predation cues affected neither CORT release rate nor bufadienolide content. Our results suggest that changes in bufadienolide synthesis in response to environmental challenges are not driven by CORT but may rather be regulated by upstream hormones of the stress response.}, year = {2023}, eissn = {2517-4843}, pages = {1-13}, orcid-numbers = {Üveges, Bálint/0000-0001-9234-9258; Gabor, C R/0000-0001-7584-1451; Bókony, Veronika/0000-0002-2136-5346} } @CONFERENCE{MTMT:33661348, title = {A klímaváltozás és két piretroid rovarölőszer együttes hatásai juvenilis barna varangyok (Bufo bufo) fejlődésére és túlélésére}, url = {https://m2.mtmt.hu/api/publication/33661348}, author = {Kásler, Andrea and Bókony, Veronika and Mikó, Zsanett and Herczeg, Dávid and Ujszegi, János and Holly, Dóra and Hettyey, Attila}, booktitle = {69. NÖVÉNYVÉDELMI TUDOMÁNYOS NAPOK}, unique-id = {33661348}, year = {2023}, pages = {36}, orcid-numbers = {Bókony, Veronika/0000-0002-2136-5346; Herczeg, Dávid/0000-0001-5098-961X} } @CONFERENCE{MTMT:33661330, title = {Ökotoxikológia egy komplex világban: peszticidek és patogének együttes hatásai erdei békákra (Rana dalmatina) a korai életszakaszban}, url = {https://m2.mtmt.hu/api/publication/33661330}, author = {Hettyey, Attila and Kásler, Andrea and Ujszegi, János and Holly, Dóra and Herczeg, Dávid}, booktitle = {69. NÖVÉNYVÉDELMI TUDOMÁNYOS NAPOK}, unique-id = {33661330}, year = {2023}, pages = {34}, orcid-numbers = {Herczeg, Dávid/0000-0001-5098-961X} } @article{MTMT:33657273, title = {Toxicity of POEA-containing glyphosate-based herbicides to amphibians is mainly due to the surfactant, not to the active ingredient}, url = {https://m2.mtmt.hu/api/publication/33657273}, author = {Mikó, Zsanett and Hettyey, Attila}, doi = {10.1007/s10646-023-02626-x}, journal-iso = {ECOTOXICOLOGY}, journal = {ECOTOXICOLOGY}, volume = {32}, unique-id = {33657273}, issn = {0963-9292}, abstract = {Current international legislation regarding agrochemicals requires thorough toxicological testing mainly of the active ingredients. In a 96-h acute toxicity test we exposed Rana dalmatina and Bufo bufo tadpoles to either one of three concentrations of glyphosate, three concentrations of the surfactant (POEA), three concentrations of the two components together, or to non-contaminated water (control), and subsequently assessed mortality and body mass. To investigate whether simultaneous exposure to another stress factor influences effects of the contaminants, we performed tests both in the presence or absence of predator chemical cues. We found that the surfactant had significant harmful effects on tadpoles; survival was lowered by the highest concentration of the surfactant in case of R. dalmatina , while in B. bufo tadpoles it reduced survival already at medium concentrations. Body mass was significantly influenced by medium and high surfactant concentrations in both species. The presence of glyphosate did not have a significant effect by itself, but it slightly increased mortality in tadpoles exposed to medium concentrations of the surfactant in both species. The presence of chemical cues did not have an effect on the examined variables. Our study confirms that the toxicity of glyphosate-based herbicides is mainly due to the examined surfactant. Nonetheless, we found that glyphosate can enhance the harmful effect of the surfactant. These results stress that during the authorization process of new pesticide formulations, not only the active ingredients would need to be examined but the excipients should also be taken into account in an obligatory and systematic manner.}, year = {2023}, eissn = {1573-3017}, pages = {150-159} }