TY - JOUR AU - Márkus, Róbert AU - Lerner, Zita AU - Honti, Viktor AU - Csordás, Gábor AU - Zsámboki, János AU - Cinege, Gyöngyi Ilona AU - Párducz, Árpád AU - Lukacsovich, Tamás AU - Kurucz, Judit Éva AU - Andó, István TI - Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 7 PY - 2015 IS - 4 SP - 340 EP - 353 PG - 14 SN - 1662-811X DO - 10.1159/000369618 UR - https://m2.mtmt.hu/api/publication/2853634 ID - 2853634 N1 - Megjegyzés-25258522 Hiányzó Jelleg: 'JOUR\n\nArticle' Admin megjegyzés-25258522 tblcategory: (Category) ('JOUR\n\nArticle') #Jelleg AB - We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. © 2015 S. Karger AG, Basel LA - English DB - MTMT ER - TY - CHAP AU - Varga, Gergely István AU - Honti, Viktor AU - Csordás, Gábor AU - Jankovics, Ferenc AU - Márkus, Róbert AU - Lukacsovich, Tamás AU - Kurucz, Judit Éva AU - Andó, István ED - Vágvölgyi, Csaba ED - Szekeres, András TI - Vérsejt-differenciálódás vizsgálata a Drosophila melanogaster hematopoietikus kompartmentumaiban T2 - A biológia jövője, a jövő biológusai PB - JATEPress CY - Szeged SN - 9789633152126 PY - 2014 SP - 103 EP - 113 PG - 11 UR - https://m2.mtmt.hu/api/publication/2765598 ID - 2765598 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Andó, István TI - The cell-mediated immunity of Drosophila melanogaster: Hemocyte lineages, immune compartments, microanatomy and regulation. JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 42 PY - 2014 IS - 1 SP - 47 EP - 56 PG - 10 SN - 0145-305X DO - 10.1016/j.dci.2013.06.005 UR - https://m2.mtmt.hu/api/publication/2372553 ID - 2372553 N1 - Cited By :124 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Andó, I.; Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary; email: ando.istvan@brc.mta.hu AB - In the animal kingdom, innate immunity is the first line of defense against invading pathogens. The dangers of microbial and parasitic attacks are countered by similar mechanisms, involving the prototypes of the cell-mediated immune responses, the phagocytosis and encapsulation. Work on Drosophila has played an important role in promoting an understanding of the basic mechanisms of phylogenetically conserved modules of innate immunity. The aim of this review is to survey the developments in the identification and functional definition of immune cell types and the immunological compartments of Drosophila melanogaster. We focus on the molecular and developmental aspects of the blood cell types and compartments, as well as the dynamics of blood cell development and the immune response. Further advances in the characterization of the innate immune mechanisms in Drosophila will provide basic clues to the understanding of the importance of the evolutionary conserved mechanisms of innate immune defenses in the animal kingdom. LA - English DB - MTMT ER - TY - CHAP AU - Kari, Beáta AU - Zsámboki, János AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Andó, István AU - Kurucz, Judit Éva ED - Horváth, József ED - Monostori, Tamás TI - A SZEPTIKUS SÉRÜLÉST KÖVETŐ IMMUNVÁLASZT SZABÁLYOZÓ GENETIKAI FAKTOROK AZONOSÍTÁSÁNAK MÓDSZEREI DROSOPHILÁBAN T2 - Tudomány a vidék mindennapjaiban PB - Szegedi Tudományegyetem Mezőgazdasági Kar CY - Hódmezővásárhely SN - 9789633062456 PY - 2013 SP - 43 EP - 47 PG - 5 UR - https://m2.mtmt.hu/api/publication/2519814 ID - 2519814 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Csordás, Gábor AU - Honti, Viktor AU - Márkus, Róbert AU - Jankovics, Ferenc AU - Varga, Gergely István AU - Kurucz, Judit Éva AU - Andó, István ED - Horváth, József ED - Monostori, Tamás TI - AZ ECETMUSLICA (DROSOPHILA MELANOGASTER) VÉRSEJTKÉPZŐDÉSE T2 - Tudomány a vidék mindennapjaiban PB - Szegedi Tudományegyetem Mezőgazdasági Kar CY - Hódmezővásárhely SN - 9789633062456 PY - 2013 SP - 23 EP - 28 PG - 6 UR - https://m2.mtmt.hu/api/publication/2519808 ID - 2519808 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Lipinszki, Zoltán AU - Klement, Éva AU - Hunyadi-Gulyás Éva, Csilla AU - Medzihradszky F., Katalin AU - Márkus, Róbert AU - Pál, Margit AU - Deák, Péter AU - Udvardy, Andor TI - A novel interplay between the ubiquitin-proteasome system and serine proteases during Drosophila development. JF - BIOCHEMICAL JOURNAL J2 - BIOCHEM J VL - 454 PY - 2013 IS - 3 SP - 571 EP - 583 PG - 13 SN - 0264-6021 DO - 10.1042/BJ20130040 UR - https://m2.mtmt.hu/api/publication/2490590 ID - 2490590 N1 - Megjegyzés-24101922 PN 3 Megjegyzés-24098840 PN 3 Megjegyzés-24101552 PN 3 Megjegyzés-24093510 PN 3 \n Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary \n Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary \n Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom \n Cited By :3 \n Export Date: 1 December 2018 \n CODEN: BIJOA \n Correspondence Address: Lipinszki, Z.; Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom; email: zl295@cam.ac.uk Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom Cited By :3 Export Date: 20 October 2020 CODEN: BIJOA Correspondence Address: Lipinszki, Z.; Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom; email: zl295@cam.ac.uk AB - The concentrations of the Drosophila proteasomal and extraproteasomal polyubiquitin receptors fluctuate in a developmentally regulated fashion. This fluctuation is generated by a previously unidentified proteolytic activity. In the present paper, we describe the purification, identification and characterization of this protease (endoproteinase I). Its expression increases sharply at the L1-L2 larval stages, remains high until the second half of the L3 stage, then declines dramatically. This sharp decrease coincides precisely with the increase of polyubiquitin receptor concentrations in late L3 larvae, which suggests a tight developmental co-regulation. RNAi-induced down-regulation of endoproteinase I results in pupal lethality. Interestingly, we found a cross-talk between the 26S proteasome and this larval protease: transgenic overexpression of the in vivo target of endoproteinase I, the C-terminal half of the proteasomal polyubiquitin receptor subunit p54/Rpn10 results in transcriptional down-regulation of endoproteinase I and consequently a lower level of proteolytic elimination of the polyubiquitin receptors. Another larval protease, Jonah65A-IV, which degrades only unfolded proteins and exhibits similar cross-talk with the proteasome has also been purified and characterized. It may prevent the accumulation of polyubiquitylated proteins in larvae contrary to the low polyubiquitin receptor concentration. LA - English DB - MTMT ER - TY - JOUR AU - Kari, Beáta AU - Zsámboki, János AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Andó, István AU - Kurucz, Judit Éva TI - A novel method for the identification of factors involved in host-pathogen interactions in Drosophila melanogaster. JF - JOURNAL OF IMMUNOLOGICAL METHODS J2 - J IMMUNOL METHODS VL - 398-399 PY - 2013 SP - 76 EP - 82 PG - 7 SN - 0022-1759 DO - 10.1016/j.jim.2013.09.011 UR - https://m2.mtmt.hu/api/publication/2463198 ID - 2463198 N1 - WoS:hiba:000329011500009 2019-03-03 20:54 kötet nem egyezik AB - A new method was established, standardized and validated for screening factors involved in the response to septic injury in Drosophila melanogaster. The method, based on inducing lesion by removing the tarsal segments of the first pair of legs of Drosophila adults and exposing them to different bacteria, imitates injury that often occurs in the natural habitat. The method is easy to perform, highly reproducible and suitable for large-scale genetic screens with the aim of identifying factors involved in host-pathogen interactions. The technique was validated by using mutant variations of different components of the immune response, blood clotting as well as the involvement of a number of genes known to be instrumental in the humoral and cell-mediated immune responses of Drosophila was confirmed. Moreover, the combination of the present method with antibiotic treatment allows the screening of potential antimicrobial drugs in vivo. LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Kurucz, Judit Éva AU - Jankovics, Ferenc AU - Andó, István TI - Cell lineage tracing reveals the plasticity of the hemocyte lineages and of the hematopoietic compartments in drosophila melanogaster JF - MOLECULAR IMMUNOLOGY J2 - MOL IMMUNOL VL - 47 PY - 2010 IS - 11-12 SP - 1997 EP - 2004 PG - 8 SN - 0161-5890 DO - 10.1016/j.molimm.2010.04.017 UR - https://m2.mtmt.hu/api/publication/1921048 ID - 1921048 N1 - Cited By :83 Export Date: 19 January 2022 CODEN: IMCHA Correspondence Address: Andó, I.; Institute of Genetics, Temesvári krt. 62, 6726 Szeged, Csongrád, Hungary; email: ando@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Csordás, Gábor AU - Laurinyecz, Barbara AU - Márkus, Róbert AU - Andó, István TI - In vivo detection of lamellocytes in Drosophila melanogaster JF - IMMUNOLOGY LETTERS J2 - IMMUNOL LETT VL - 126 PY - 2009 IS - 1-2 SP - 83 EP - 84 PG - 2 SN - 0165-2478 DO - 10.1016/j.imlet.2009.08.004 UR - https://m2.mtmt.hu/api/publication/1920812 ID - 1920812 N1 - Cited By :27 Export Date: 30 June 2022 CODEN: IMLED LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert TI - Csodálatos apróságok a mikroszkóp alatt JF - DIGITÁLIS FOTÓ MAGAZIN J2 - DIGITÁLIS FOTÓ MAGAZIN VL - 9 PY - 2009 IS - 5 SP - 80 EP - 93 PG - 14 SN - 1587-7280 UR - https://m2.mtmt.hu/api/publication/1920807 ID - 1920807 LA - Hungarian DB - MTMT ER -