@article{MTMT:34850818,
	title = {Development and implementation of a novel CAPS assay reveals high prevalence of a boscalid resistance marker and its co-occurrence with an azole resistance marker in Erysiphe necator},
	url = {https://m2.mtmt.hu/api/publication/34850818},
	author = {Seress, Diána and Molnár, Orsolya and Matolcsi, Fruzsina and Pintye, Alexandra and Kovács, M. Gábor and Németh, Márk Z.},
	doi = {10.1094/PDIS-06-23-1114-SR},
	journal-iso = {PLANT DIS},
	journal = {PLANT DISEASE},
	unique-id = {34850818},
	issn = {0191-2917},
	abstract = {Succinate dehydrogenase inhibitors (SDHIs), are frequently used against powdery mildew (PM) fungi, such as Erysiphe necator, the causal agent of grapevine PM. Fungicide resistance, however, hinders effective control. DNA-based monitoring facilitates the recognition of resistance. We aimed (i) to adapt an effective method to detect a widespread genetic marker of resistance to boscalid, a commonly used SDHI, and (ii) to study the co-occurrence of the marker with a marker of resistance to demethylase inhibitor (DMI) fungicides. Sequencing of the sdhB gene identified a non-synonymous substitution, denoted as sdhB-A794G, leading to an amino acid change (H242R) in the sdhB protein. In vitro fungicide resistance tests showed that E. necator isolates carrying sdhB-A794G were resistant to boscalid. We adopted a cleaved amplified polymorphic sequence-based method and screened more than 500 field samples collected from five Hungarian wine regions in two consecutive years. The sdhB-A794G marker was detected in all wine regions and in both years, altogether in 61.7% of samples, including 20.5% in which both sdhB-A794G and the wild-type were present. The frequency of sdhB-A794G was higher in SDHI-treated vineyards than in vineyards without any SDHI application. A significant difference in the presence of the marker was detected among wine regions; its prevalence ranged from none to 100%. We identified significant co-occurrence of sdhB-A794G with the CYP51-A495T (Y136F) mutation of the CYP51 gene, a known marker of resistance to DMIs. The monitoring of fungicide resistance is fundamental for the successful control of E. necator. Our rapid, cost-effective diagnostic method will support decision-making and fungicide resistance monitoring and management.},
	year = {2024},
	eissn = {1943-7692},
	orcid-numbers = {Kovács, M. Gábor/0000-0001-9509-4270}
}

@article{MTMT:34849835,
	title = {Revisiting the intron hypothesis of QoI resistance in Phyllosticta ampelicida , the causal agent of grape black rot, and other Phyllosticta species},
	url = {https://m2.mtmt.hu/api/publication/34849835},
	author = {Horváth, Áron N. and Molnár, Orsolya and Németh, Márk Z. and Pintye, Alexandra and Dankó, Tamás and Spitzmüller, Zsolt and Váczy, Zsuzsanna and Váczy, Kálmán Zoltán and Onesti, Giovanni and Reis, Pedro and Rego, Cecilia and Bereczky, Zsolt and Kiss, Levente and Kovács, M. Gábor},
	doi = {10.1111/ppa.13912},
	journal-iso = {PLANT PATHOL},
	journal = {PLANT PATHOLOGY},
	unique-id = {34849835},
	issn = {0032-0862},
	abstract = {Chemical control of grape black rot, caused by Phyllosticta ampelicida , relies mainly on the use of demethylation inhibitors (DMIs) and quinone outside inhibitors (QoIs). The effectiveness of QoI fungicides is influenced by alternative respiration activity, and the exon/intron structure and point mutations in the target protein's gene, the cytochrome b ( cytb ) gene. Our study aims to investigate the QoI fungicide sensitivity of 48 P . ampelicida isolates in vitro by measuring EC 50 and the molecular characteristics of the cytb gene and its mRNA in P . ampelicida and other Phyllosticta species. Mycelial growth tests revealed that the P . ampelicida isolates were sensitive to both azoxystrobin and trifloxystrobin; baseline EC 50 values were 0.029 and 0.022 μg/mL, respectively. Addition of salicylhydroxamic acid (SHAM) resulted in lower EC 50 values (0.024 and 0.017 μg/mL, respectively). None of the typical point mutations conferring resistance to QoIs in some fungi were detected. A group I intron was present right after the 143rd codon in the cytb gene in four of the six Phyllosticta species examined. The sequence and exon/intron structure of the cytb gene of P .  ampelicida isolated from Vitis vinifera is studied in detail and published here. Our results indicate a low risk of QoI resistance development via the G143A mutation in P . ampelicida .},
	year = {2024},
	eissn = {1365-3059},
	orcid-numbers = {Molnár, Orsolya/0000-0002-9164-3997; Németh, Márk Z./0000-0001-9521-8421; Pintye, Alexandra/0000-0003-4126-7978; Váczy, Kálmán Zoltán/0000-0002-8303-5887; Reis, Pedro/0000-0001-6344-7916; Rego, Cecilia/0000-0001-8952-537X; Kiss, Levente/0000-0002-4785-4308; Kovács, M. Gábor/0000-0001-9509-4270}
}

@CONFERENCE{MTMT:34685715,
	title = {A szőlőlisztharmat kórokozó (Erysiphe necator) sdhi-fungicidekkel szembeni rezisztenciája az Egri Borvidéken},
	url = {https://m2.mtmt.hu/api/publication/34685715},
	author = {SPITZMÜLLER, ZS. and KISS, T. and KARÁCSONY-PÁLFI, X. and Pintye, Alexandra and Molnár, Orsolya and Matolcsi, Fruzsina and Németh Z., Márk and Kiss, Levente and Kovács, M. Gábor and Váczy, Kálmán Zoltán},
	booktitle = {70. Növényvédelmi Tudományos Napok},
	unique-id = {34685715},
	year = {2024},
	pages = {74},
	orcid-numbers = {Molnár, Orsolya/0000-0002-9164-3997; Németh Z., Márk/0000-0001-9521-8421; Kovács, M. Gábor/0000-0001-9509-4270}
}

@CONFERENCE{MTMT:34685628,
	title = {Fuzáriumok magyarországi ciprusfélék rákbetegség tüneteit mutató hajtásaiból},
	url = {https://m2.mtmt.hu/api/publication/34685628},
	author = {Molnár, Orsolya and Németh Z., Márk and Bozsik, Gábor and Seress, Diána and Kovács, M. Gábor and Szőcs, Gábor},
	booktitle = {70. Növényvédelmi Tudományos Napok},
	unique-id = {34685628},
	year = {2024},
	pages = {38},
	orcid-numbers = {Molnár, Orsolya/0000-0002-9164-3997; Németh Z., Márk/0000-0001-9521-8421; Kovács, M. Gábor/0000-0001-9509-4270}
}

@article{MTMT:34579676,
	title = {Trans-kingdom fungal pathogens infecting both plants and humans, and the problem of azole fungicide resistance},
	url = {https://m2.mtmt.hu/api/publication/34579676},
	author = {Pintye, Alexandra and Bacsó, Renáta and Kovács, M. Gábor},
	doi = {10.3389/fmicb.2024.1354757},
	journal-iso = {FRONT MICROBIOL},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {15},
	unique-id = {34579676},
	issn = {1664-302X},
	abstract = {Azole antifungals are abundantly used in the environment and play an important role in managing fungal diseases in clinics. Due to the widespread use, azole resistance is an emerging global problem for all applications in several fungal species, including trans-kingdom pathogens, capable of infecting plants and humans. Azoles used in agriculture and clinics share the mode of action and facilitating cross-resistance development. The extensive use of azoles in the environment, e.g., for plant protection and wood preservation, contributes to the spread of resistant populations and challenges using these antifungals in medical treatments. The target of azoles is the cytochrome p450 lanosterol 14-α demethylase encoded by the CYP51 (called also as ERG11 in the case of yeasts) gene. Resistance mechanisms involve mainly the mutations in the coding region in the CYP51 gene, resulting in the inadequate binding of azoles to the encoded Cyp51 protein, or mutations in the promoter region causing overexpression of the protein. The World Health Organization (WHO) has issued the first fungal priority pathogens list (FPPL) to raise awareness of the risk of fungal infections and the increasingly rapid spread of antifungal resistance. Here, we review the main issues about the azole antifungal resistance of trans-kingdom pathogenic fungi with the ability to cause serious human infections and included in the WHO FPPL. Methods for the identification of these species and detection of resistance are summarized, highlighting the importance of these issues to apply the proper treatment.},
	year = {2024},
	eissn = {1664-302X},
	orcid-numbers = {Kovács, M. Gábor/0000-0001-9509-4270}
}

@article{MTMT:34411893,
	title = {Exploring diversity within the genus Tulostoma (Basidiomycota, Agaricales) in the Pannonian sandy steppe: four fascinating novel species from Hungary},
	url = {https://m2.mtmt.hu/api/publication/34411893},
	author = {Finy, Péter and Jeppson, Mikael and Knapp, Dániel and Papp, Viktor and Albert, László and Ölvedi, István and Bóka, Károly and Varga, Dóra and Kovács, M. Gábor and Dima, Bálint},
	doi = {10.3897/mycokeys.100.112458},
	journal-iso = {MYCOKEYS},
	journal = {MYCOKEYS},
	volume = {100},
	unique-id = {34411893},
	issn = {1314-4057},
	abstract = {Steppe vegetation on sandy soil in Hungary has recently been revealed as one of the hot spots in Europe for the stalked puffballs (genus Tulostoma ). In the framework of the taxonomic revision of gasteroid fungi in Hungary, four Tulostoma species are described here as new to science: T. dunense , T. hungaricum , T. sacchariolens and T. shaihuludii . The study is based on detailed macro- and micromorphological investigations (including light and scanning electron microscopy), as well as a three-locus phylogeny of nrDNA ITS, nrDNA LSU and tef1-α sequences. The ITS and LSU sequences generated from the type specimen of T. cretaceum are provided and this resolved partly the taxonomy of the difficult species complex of T. aff. cretaceum.},
	year = {2023},
	eissn = {1314-4049},
	pages = {153-170},
	orcid-numbers = {Knapp, Dániel/0000-0002-7568-238X; Bóka, Károly/0000-0002-1324-3592; Kovács, M. Gábor/0000-0001-9509-4270; Dima, Bálint/0000-0003-2099-3903}
}

@article{MTMT:34281522,
	title = {Untargeted metabolomic analyses support the main phylogenetic groups of the common plant-associated Alternaria fungi isolated from grapevine (Vitis vinifera)},
	url = {https://m2.mtmt.hu/api/publication/34281522},
	author = {Molnár, Anna and Knapp, Dániel and Lovas, Miklós and Tóth, Gergő and Boldizsár, Imre and Váczy, Kálmán Zoltán and Kovács, M. Gábor},
	doi = {10.1038/s41598-023-46020-3},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {13},
	unique-id = {34281522},
	issn = {2045-2322},
	abstract = {Alternaria , a cosmopolitan fungal genus is a dominant member of the grapevine ( Vitis vinifera ) microbiome. Several Alternaria species are known to produce a variety of secondary metabolites, which are particularly relevant to plant protection and food safety in field crops. According to previous findings, the majority of Alternaria species inhabiting grapevine belong to Alternaria sect. Alternaria . However, the phylogenetic diversity and secondary metabolite production of the distinct Alternaria species has remained unclear. In this study, our aim was to examine the genetic and metabolic diversity of endophytic Alternaria isolates associated with the above-ground tissues of the grapevine. Altogether, 270 Alternaria isolates were collected from asymptomatic leaves and grape clusters of different grapevine varieties in the Eger wine region of Hungary. After analyses of the nuclear ribosomal DNA internal transcribed spacer (ITS) and RNA polymerase second largest subunit ( rpb2 ) sequences, 170 isolates were chosen for further analyses. Sequences of the Alternaria major allergen gene ( Alt a 1 ), endopolygalacturonase ( endoPG ), OPA10-2, and KOG1058 were also included in the phylogenetic analyses. Identification of secondary metabolites and metabolite profiling of the isolates were performed using high-performance liquid chromatography (HPLC)–high-resolution tandem mass spectrometry (HR-MS/MS). The multilocus phylogeny results revealed two distinct groups in grapevine, namely A . alternata and the A . arborescens species complex (AASC). Eight main metabolites were identified in all collected Alternaria isolates, regardless of their affiliation to the species and lineages. Multivariate analyses of untargeted metabolites found no clear separations; however, a partial least squares-discriminant analysis model was able to successfully discriminate between the metabolic datasets from isolates belonging to the AASC and A. alternata . By conducting univariate analysis based on the discriminant ability of the metabolites, we also identified several features exhibiting large and significant variation between A. alternata and the AASC. The separation of these groups may suggest functional differences, which may also play a role in the functioning of the plant microbiome.},
	year = {2023},
	eissn = {2045-2322},
	orcid-numbers = {Molnár, Anna/0000-0002-0919-0512; Knapp, Dániel/0000-0002-7568-238X; Tóth, Gergő/0000-0001-5341-319X; Boldizsár, Imre/0000-0001-7852-8364; Kovács, M. Gábor/0000-0001-9509-4270}
}

@article{MTMT:34139263,
	title = {Comprehensive analyses of the occurrence of a fungicide resistance marker and the genetic structure in Erysiphe necator populations},
	url = {https://m2.mtmt.hu/api/publication/34139263},
	author = {Pintye, Alexandra and Németh Z., Márk and Molnár, Orsolya and Horváth, Áron and Matolcsi, Fruzsina and Bókony, Veronika and Spitzmüller, Zsolt and Pálfi, Xénia and Váczy, Kálmán Zoltán and Kovács, M. Gábor},
	doi = {10.1038/s41598-023-41454-1},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {13},
	unique-id = {34139263},
	issn = {2045-2322},
	abstract = {Genetically distinct groups of Erysiphe necator , the fungus causing grapevine powdery mildew infect grapevine in Europe, yet the processes sustaining stable genetic differences between those groups are less understood. Genotyping of over 2000 field samples from six wine regions in Hungary collected between 2017 and 2019 was conducted to reveal E. necator genotypes and their possible differentiation. The demethylase inhibitor (DMI) fungicide resistance marker A495T was detected in all wine regions, in 16% of the samples. Its occurrence differed significantly among wine regions and grape cultivars, and sampling years, but it did not differ between DMI-treated and untreated fields. Multilocus sequence analyses of field samples and 59 in vitro maintained isolates revealed significant genetic differences among populations from distinct wine regions. We identified 14 E. necator genotypes, of which eight were previously unknown. In contrast to the previous concept of A and B groups, European E. necator populations should be considered genetically more complex. Isolation by geographic distance, growing season, and host variety influence the genetic structuring of E. necator , which should be considered both during diagnoses and when effective treatments are planned.},
	year = {2023},
	eissn = {2045-2322},
	orcid-numbers = {Németh Z., Márk/0000-0001-9521-8421; Molnár, Orsolya/0000-0002-9164-3997; Bókony, Veronika/0000-0002-2136-5346; Kovács, M. Gábor/0000-0001-9509-4270}
}

@CONFERENCE{MTMT:34122754,
	title = {TAXONOMIC AND METABOLIC CHARACTERIZATION OF ALTERNARIA SPECIES IN GRAPEVINE (VITIS VINIFERA) IN HUNGARY},
	url = {https://m2.mtmt.hu/api/publication/34122754},
	author = {Molnár, Anna and Knapp, Dániel and Lovas, Miklós and Boldizsár, Imre and Tóth, Gergő and Váczy, Kálmán Zoltán and Kovács, M. Gábor},
	booktitle = {Abstracts of the 19th International Congress of the Hungarian Society for Microbiology},
	unique-id = {34122754},
	year = {2023},
	pages = {72-72},
	orcid-numbers = {Molnár, Anna/0000-0002-0919-0512; Knapp, Dániel/0000-0002-7568-238X; Boldizsár, Imre/0000-0001-7852-8364; Tóth, Gergő/0000-0001-5341-319X; Kovács, M. Gábor/0000-0001-9509-4270}
}

@CONFERENCE{MTMT:34113193,
	title = {Prevalence and co-occurrence of two fungicide resistance markers in Erysiphe necator},
	url = {https://m2.mtmt.hu/api/publication/34113193},
	author = {Seress, Diána and Molnár, Orsolya and Pintye, Alexandra and Matolcsi, Fruzsina and Bókony, Veronika and Horváth, Áron and Kovács, M. Gábor and Németh Z., Márk},
	booktitle = {12th International Congress of Plant Pathology},
	unique-id = {34113193},
	year = {2023},
	orcid-numbers = {Molnár, Orsolya/0000-0002-9164-3997; Bókony, Veronika/0000-0002-2136-5346; Kovács, M. Gábor/0000-0001-9509-4270; Németh Z., Márk/0000-0001-9521-8421}
}