@article{MTMT:35133615, title = {Disentangling the Hippocampal Projectome}, url = {https://m2.mtmt.hu/api/publication/35133615}, author = {Varga, Viktor}, doi = {10.1007/s12264-024-01255-6}, journal-iso = {NEUROSCI BULL}, journal = {NEUROSCIENCE BULLETIN}, unique-id = {35133615}, issn = {1673-7067}, year = {2024}, eissn = {1995-8218} } @article{MTMT:35058848, title = {Microglia contribute to neuronal synchrony despite endogenous ATP-related phenotypic transformation in acute mouse brain slices}, url = {https://m2.mtmt.hu/api/publication/35058848}, author = {Berki, Péter and Cserép, Csaba and Környei, Zsuzsanna and Pósfai, Balázs and Cserépné Szabadits, Eszter and Domonkos, Andor and Kellermayer, Anna and Nyerges, Miklós and Wei, Xiaofei and Mody, Istvan and Kunihiko, Araki and Beck, Heinz and Kaikai, He and Ya, Wang and Lénárt, Nikolett and Wu, Zhaofa and Jing, Miao and Li, Yulong and Gulyás, Attila and Dénes, Ádám}, doi = {10.1038/s41467-024-49773-1}, journal-iso = {NAT COMMUN}, journal = {NATURE COMMUNICATIONS}, volume = {15}, unique-id = {35058848}, issn = {2041-1723}, year = {2024}, eissn = {2041-1723}, orcid-numbers = {Cserép, Csaba/0000-0001-5513-2471; Pósfai, Balázs/0000-0003-1035-565X; Lénárt, Nikolett/0000-0002-7456-949X; Gulyás, Attila/0000-0003-4961-636X} } @article{MTMT:35050908, title = {Parvalbumin-expressing basal forebrain neurons mediate learning from negative experience}, url = {https://m2.mtmt.hu/api/publication/35050908}, author = {Hegedüs, Panna and Király, Bálint and Schlingloff, Dániel and Lyakhova, Victoria and Velencei, Anna and Szabó, Írisz and Mayer, Márton István and Zelenak, Zsofia and Nyíri, Gábor and Hangya, Balázs}, doi = {10.1038/s41467-024-48755-7}, journal-iso = {NAT COMMUN}, journal = {NATURE COMMUNICATIONS}, volume = {15}, unique-id = {35050908}, issn = {2041-1723}, abstract = {Parvalbumin (PV)-expressing GABAergic neurons of the basal forebrain (BFPVNs) were proposed to serve as a rapid and transient arousal system, yet their exact role in awake behaviors remains unclear. We performed bulk calcium measurements and electrophysiology with optogenetic tagging from the horizontal limb of the diagonal band of Broca (HDB) while male mice were performing an associative learning task. BFPVNs responded with a distinctive, phasic activation to punishment, but showed slower and delayed responses to reward and outcome-predicting stimuli. Optogenetic inhibition during punishment impaired the formation of cue-outcome associations, suggesting a causal role of BFPVNs in associative learning. BFPVNs received strong inputs from the hypothalamus, the septal complex and the median raphe region, while they synapsed on diverse cell types in key limbic structures, where they broadcasted information about aversive stimuli. We propose that the arousing effect of BFPVNs is recruited by aversive stimuli to serve crucial associative learning functions.}, year = {2024}, eissn = {2041-1723}, orcid-numbers = {Hegedüs, Panna/0000-0002-9984-5729} } @article{MTMT:35050868, title = {Early and selective localization of tau filaments to glutamatergic subcellular domains within the human anterodorsal thalamus}, url = {https://m2.mtmt.hu/api/publication/35050868}, author = {Sárkány, Barbara and Dávid, Csaba and Hortobágyi, Tibor and Gombás, Péter and Somogyi, Peter and Acsády, László and Viney, Tim J.}, doi = {10.1007/s00401-024-02749-3}, journal-iso = {ACTA NEUROPATHOL}, journal = {ACTA NEUROPATHOLOGICA}, volume = {147}, unique-id = {35050868}, issn = {0001-6322}, abstract = {Widespread cortical accumulation of misfolded pathological tau proteins (ptau) in the form of paired helical filaments is a major hallmark of Alzheimer’s disease. Subcellular localization of ptau at various stages of disease progression is likely to be informative of the cellular mechanisms involving its spread. Here, we found that the density of ptau within several distinct rostral thalamic nuclei in post-mortem human tissue ( n = 25 cases) increased with the disease stage, with the anterodorsal nucleus (ADn) consistently being the most affected. In the ADn, ptau-positive elements were present already in the pre-cortical (Braak 0) stage. Tau pathology preferentially affected the calretinin-expressing subpopulation of glutamatergic neurons in the ADn. At the subcellular level, we detected ptau immunoreactivity in ADn cell bodies, dendrites, and in a specialized type of presynaptic terminal that expresses vesicular glutamate transporter 2 (vGLUT2) and likely originates from the mammillary body. The ptau-containing terminals displayed signs of degeneration, including endosomal/lysosomal organelles. In contrast, corticothalamic axon terminals lacked ptau. The data demonstrate the involvement of a specific cell population in ADn at the onset of the disease. The presence of ptau in subcortical glutamatergic presynaptic terminals supports hypotheses about the transsynaptic spread of tau selectively affecting specialized axonal pathways.}, year = {2024}, eissn = {1432-0533}, orcid-numbers = {Viney, Tim J./0000-0001-6444-1188} } @article{MTMT:35050690, title = {Intracortical mechanisms of single pulse electrical stimulation (SPES) evoked excitations and inhibitions in humans.}, url = {https://m2.mtmt.hu/api/publication/35050690}, author = {Hajnal, Boglárka Zsófia and Szabó, Johanna Petra and Tóth, Emília and Keller, Corey J and Wittner, Lucia and Mehta, Ashesh D and Erőss, Loránd and Ulbert, István and Fabó, Dániel and Entz, László}, doi = {10.1038/s41598-024-62433-0}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {35050690}, issn = {2045-2322}, abstract = {Cortico-cortical evoked potentials (CCEPs) elicited by single-pulse electric stimulation (SPES) are widely used to assess effective connectivity between cortical areas and are also implemented in the presurgical evaluation of epileptic patients. Nevertheless, the cortical generators underlying the various components of CCEPs in humans have not yet been elucidated. Our aim was to describe the laminar pattern arising under SPES evoked CCEP components (P1, N1, P2, N2, P3) and to evaluate the similarities between N2 and the downstate of sleep slow waves. We used intra-cortical laminar microelectrodes (LMEs) to record CCEPs evoked by 10 mA bipolar 0.5 Hz electric pulses in seven patients with medically intractable epilepsy implanted with subdural grids. Based on the laminar profile of CCEPs, the latency of components is not layer-dependent, however their rate of appearance varies across cortical depth and stimulation distance, while the seizure onset zone does not seem to affect the emergence of components. Early neural excitation primarily engages middle and deep layers, propagating to the superficial layers, followed by mainly superficial inhibition, concluding in a sleep slow wave-like inhibition and excitation sequence.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Szabó, Johanna Petra/0000-0001-7690-9171; Wittner, Lucia/0000-0001-6800-0953; Erőss, Loránd/0000-0002-5796-5546; Ulbert, István/0000-0001-9941-9159; Fabó, Dániel/0000-0001-5141-5351} } @article{MTMT:34939308, title = {Characterization of dUTPase expression in mouse postnatal development and adult neurogenesis}, url = {https://m2.mtmt.hu/api/publication/34939308}, author = {Nagy, Nikolett and Hádinger, Nóra and Tóth, Otília and Rácz, Gergely Attila and Pintér, Tímea and Gál, Zoltán and Urbán, Martin and Gócza, Elen and Hiripi, László and Acsády, László and Vértessy, Beáta (Grolmuszné)}, doi = {10.1038/s41598-024-63405-0}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34939308}, issn = {2045-2322}, abstract = {The enzyme dUTPase has an essential role in maintaining genomic integrity. In mouse, nuclear and mitochondrial isoforms of the enzyme have been described. Here we present the isoform-specific mRNA expression levels in different murine organs during development using RT-qPCR. In this study, we analyzed organs of 14.5-day embryos and of postnatal 2-, 4-, 10-week- and 13-month-old mice. We demonstrate organ-, sex- and developmental stage-specific differences in the mRNA expression levels of both isoforms. We found high mRNA expression level of the nuclear isoform in the embryo brain, and the expression level remained relatively high in the adult brain as well. This was surprising, since dUTPase is known to play an important role in proliferating cells, and mass production of neural cells is completed by adulthood. Thus, we investigated the pattern of the dUTPase protein expression specifically in the adult brain with immunostaining and found that dUTPase is present in the germinative zones, the subventricular and the subgranular zones, where neurogenesis occurs and in the rostral migratory stream where neuroblasts migrate to the olfactory bulb. These novel findings suggest that dUTPase may have a role in cell differentiation and indicate that accurate dTTP biosynthesis can be vital, especially in neurogenesis.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Rácz, Gergely Attila/0000-0002-8051-8269} } @article{MTMT:34833183, title = {Different states of synaptic vesicle priming explain target cell type–dependent differences in neurotransmitter release}, url = {https://m2.mtmt.hu/api/publication/34833183}, author = {Aldahabi, Mohammad and Neher, Erwin and Nusser, Zoltán}, doi = {10.1073/pnas.2322550121}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {121}, unique-id = {34833183}, issn = {0027-8424}, abstract = {Pronounced differences in neurotransmitter release from a given presynaptic neuron, depending on the synaptic target, are among the most intriguing features of cortical networks. Hippocampal pyramidal cells (PCs) release glutamate with low probability to somatostatin expressing oriens-lacunosum-moleculare (O-LM) interneurons (INs), and the postsynaptic responses show robust short-term facilitation, whereas the release from the same presynaptic axons onto fast-spiking INs (FSINs) is ~10-fold higher and the excitatory postsynaptic currents (EPSCs) display depression. The mechanisms underlying these vastly different synaptic behaviors have not been conclusively identified. Here, we applied a combined functional, pharmacological, and modeling approach to address whether the main difference lies in the action potential-evoked fusion or else in upstream priming processes of synaptic vesicles (SVs). A sequential two-step SV priming model was fitted to the peak amplitudes of unitary EPSCs recorded in response to complex trains of presynaptic stimuli in acute hippocampal slices of adult mice. At PC–FSIN connections, the fusion probability (P fusion ) of well-primed SVs is 0.6, and 44% of docked SVs are in a fusion-competent state. At PC–O-LM synapses, P fusion is only 40% lower (0.36), whereas the fraction of well-primed SVs is 6.5-fold smaller. Pharmacological enhancement of fusion by 4-AP and priming by PDBU was recaptured by the model with a selective increase of P fusion and the fraction of well-primed SVs, respectively. Our results demonstrate that the low fidelity of transmission at PC–O-LM synapses can be explained by a low occupancy of the release sites by well-primed SVs.}, keywords = {hippocampal interneurons; Synaptic modeling; short-term plasticity; synaptic diversity; active zone}, year = {2024}, eissn = {1091-6490}, orcid-numbers = {Neher, Erwin/0000-0002-9758-7922} } @article{MTMT:34749015, title = {Synaptic and dendritic architecture of different types of hippocampal somatostatin interneurons}, url = {https://m2.mtmt.hu/api/publication/34749015}, author = {Tresóné Takács, Virág and Bardóczi, Zsuzsanna and Orosz, Áron and Major, Ábel and Tar, Luca and Berki, Péter and Papp, Péter and Mayer, Márton István and Sebők, Hunor and Zsolt, Luca and Sós, Katalin Eszter and Káli, Szabolcs and Freund, Tamás and Nyíri, Gábor}, doi = {10.1371/journal.pbio.3002539}, journal-iso = {PLOS BIOL}, journal = {PLOS BIOLOGY}, volume = {22}, unique-id = {34749015}, issn = {1544-9173}, abstract = {GABAergic inhibitory neurons fundamentally shape the activity and plasticity of cortical circuits. A major subset of these neurons contains somatostatin (SOM); these cells play crucial roles in neuroplasticity, learning, and memory in many brain areas including the hippocampus, and are implicated in several neuropsychiatric diseases and neurodegenerative disorders. Two main types of SOM-containing cells in area CA1 of the hippocampus are oriens-lacunosum-moleculare (OLM) cells and hippocampo-septal (HS) cells. These cell types show many similarities in their soma-dendritic architecture, but they have different axonal targets, display different activity patterns in vivo, and are thought to have distinct network functions. However, a complete understanding of the functional roles of these interneurons requires a precise description of their intrinsic computational properties and their synaptic interactions. In the current study we generated, analyzed, and make available several key data sets that enable a quantitative comparison of various anatomical and physiological properties of OLM and HS cells in mouse. The data set includes detailed scanning electron microscopy (SEM)-based 3D reconstructions of OLM and HS cells along with their excitatory and inhibitory synaptic inputs. Combining this core data set with other anatomical data, patch-clamp electrophysiology, and compartmental modeling, we examined the precise morphological structure, inputs, outputs, and basic physiological properties of these cells. Our results highlight key differences between OLM and HS cells, particularly regarding the density and distribution of their synaptic inputs and mitochondria. For example, we estimated that an OLM cell receives about 8,400, whereas an HS cell about 15,600 synaptic inputs, about 16% of which are GABAergic. Our data and models provide insight into the possible basis of the different functionality of OLM and HS cell types and supply essential information for more detailed functional models of these neurons and the hippocampal network.}, year = {2024}, eissn = {1545-7885}, orcid-numbers = {Tresóné Takács, Virág/0000-0002-3276-4131} } @article{MTMT:34714189, title = {Working memory features are embedded in hippocampal place fields}, url = {https://m2.mtmt.hu/api/publication/34714189}, author = {Varga, Viktor and Petersen, Peter and Zutshi, Ipshita and Huszar, Roman and Zhang, Yiyao and Buzsáki, György}, doi = {10.1016/j.celrep.2024.113807}, journal-iso = {CELL REP}, journal = {CELL REPORTS}, volume = {43}, unique-id = {34714189}, issn = {2211-1247}, year = {2024}, eissn = {2211-1247}, pages = {113807} } @article{MTMT:35159077, title = {A láthatatlan diszbiózis}, url = {https://m2.mtmt.hu/api/publication/35159077}, author = {Zsoldos, Márton and Major, Ábel}, journal-iso = {KONZÍLIUM}, journal = {KONZÍLIUM}, volume = {2023}, unique-id = {35159077}, issn = {1416-1397}, abstract = {Az alábbiakban a fogorvosi ellátás során gyakran alkal- mazott antibiotikumok használatának gasztrointesztinális traktusra gyakorolt hatásaival foglalkozunk. Szeretnénk felhívni a klinikus kollégák figyelmét az antibiotikumok használatából adódó mellékhatásokra és azok elkerülé- sének, mérséklésének gyakorlati lehetőségeire.}, year = {2023}, pages = {1-5}, orcid-numbers = {Zsoldos, Márton/0009-0004-0440-2047} }