TY - JOUR AU - Magyar, Melinda AU - Akhtar, Parveen AU - Sipka, Gábor AU - Racskóné Domonkos, Ildikó AU - Han, W. AU - Li, X. AU - Han, G. AU - Shen, J.-R. AU - Lambrev, Petar AU - Garab, Győző TI - Effects of lipids on the rate-limiting steps in the dark-to-light transition of Photosystem II core complex of Thermostichus vulcanus JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 15 PY - 2024 SN - 1664-462X DO - 10.3389/fpls.2024.1381040 UR - https://m2.mtmt.hu/api/publication/34797803 ID - 34797803 N1 - Institute of Plant Biology, HUN-REN Biological Research Centre, Szeged, Hungary Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China Research Institute for Interdisciplinary Science, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan Department of Physics, Faculty of Science, University of Ostrava, Ostrava, Czech Republic Export Date: 16 April 2024 Correspondence Address: Garab, G.; Institute of Plant Biology, Hungary; email: garab.gyozo@brc.hu AB - In our earlier works, we have shown that the rate-limiting steps, associated with the dark-to-light transition of Photosystem II (PSII), reflecting the photochemical activity and structural dynamics of the reaction center complex, depend largely on the lipidic environment of the protein matrix. Using chlorophyll-a fluorescence transients (ChlF) elicited by single-turnover saturating flashes, it was shown that the half-waiting time (Δτ1/2) between consecutive excitations, at which 50% of the fluorescence increment was reached, was considerably larger in isolated PSII complexes of Thermostichus (T.) vulcanus than in the native thylakoid membrane (TM). Further, it was shown that the addition of a TM lipid extract shortened Δτ1/2 of isolated PSII, indicating that at least a fraction of the ‘missing’ lipid molecules, replaced by detergent molecules, caused the elongation of Δτ1/2. Here, we performed systematic experiments to obtain information on the nature of TM lipids that are capable of decreasing Δτ1/2. Our data show that while all lipid species shorten Δτ1/2, the negatively charged lipid phosphatidylglycerol appears to be the most efficient species – suggesting its prominent role in determining the structural dynamics of PSII reaction center. Copyright © 2024 Magyar, Akhtar, Sipka, Domonkos, Han, Li, Han, Shen, Lambrev and Garab. LA - English DB - MTMT ER - TY - JOUR AU - Rani, Vaishali AU - Shetty, Prateek AU - Maróti, Gergely TI - Comparative transcriptome study highlights the versatility of nitrogen metabolism in Chlamydomonas JF - ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS J2 - ALGAL RES VL - 79 PY - 2024 SP - 1 EP - 13 PG - 13 SN - 2211-9264 DO - 10.1016/j.algal.2024.103458 UR - https://m2.mtmt.hu/api/publication/34764951 ID - 34764951 AB - Nitrogen is an essential macronutrient and nitrate is one of the main forms of this macronutrient available for plants and microbes. Nitrate is not only the substrate for the nitrate assimilation pathway, but also a crucial signal for the regulation of numerous metabolic, developmental, and cellular differentiation processes. In the present study, two species of the Chlamydomonas genus, Chlamydomonas reinhardtii cc124 and Chlamydomonas sp. MACC-216 were used to investigate the versatility of nitrate metabolism in green microalgae. Quantification of nitrate removal efficiency showed that Chlamydomonas sp. MACC-216 strongly outperforms C. reinhardtii cc124. Transcriptional changes occurring under nitrate-replete and nitrate-deplete conditions were specifically investigated in the selected species of Chlamydomonas. Whole transcriptome analysis revealed that the genes playing a role in nitrate assimilation did not show differential expression in C. reinhardtii cc124 under changing nitrate conditions (only 45 genes exhibited differential regulation), while in Chlamydomonas sp. MACC-216 a large set of genes (3143) showed altered expression. Furthermore, genes responsible for urea metabolism, like DUR3A gene corresponding to urea transport, were found to be upregulated in Chlamydomonas sp. MACC-216 under nitrate-deplete condition, while the same gene showed elevated expression level in C. reinhardtii cc124 under nitrate-replete condition. The present study indicated the diverseness of nitrate metabolism among species within the Chlamydomonas genus. LA - English DB - MTMT ER - TY - JOUR AU - Papp, Márton AU - Tóth, Adrienn Gréta AU - Békési, László AU - Farkas, Róbert László AU - Makrai, László AU - Maróti, Gergely AU - Solymosi, Norbert TI - Apis mellifera filamentous virus from a honey bee gut microbiome survey in Hungary JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 SP - 1 EP - 8 PG - 8 SN - 2045-2322 DO - 10.1038/s41598-024-56320-x UR - https://m2.mtmt.hu/api/publication/34730183 ID - 34730183 N1 - Funding Agency and Grant Number: European Union [874735]; Hungarian Academy of Sciences [LP2020-5/2020]; NKP-22-3-II. New National Excellence program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund; University of Veterinary Medicine Funding text: In memory of Rajnald Andras Koveshegyi OCist. We would like to say thanks to the beekeepers for giving us their indispensable help. It has also received funding from the European Union's Horizon 2020 research and innovation program under Grant Agreement No. 874735 (VEO). GM received support from the Hungarian Academy of Sciences through the Lenduelet-Programme (LP2020-5/2020). Supported by the & Uacute;NKP-22-3-II. New National Excellence program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund.Open access funding provided by University of Veterinary Medicine. AB - In Hungary, as part of a nationwide, climatically balanced survey for a next-generation sequencing-based study of the honey bee (Apis mellifera) gut microbiome, repeated sampling was carried out during the honey production season (March and May 2019). Among other findings, the presence of Apis mellifera filamentous virus (AmFV) was detected in all samples, some at very high levels. AmFV-derived reads were more abundant in the March samples than in the May samples. In March, a higher abundance of AmFV-originated reads was identified in samples collected from warmer areas compared to those collected from cooler areas. A lower proportion of AmFV-derived reads were identified in samples collected in March from the wetter areas than those collected from the drier areas. AmFV-read abundance in samples collected in May showed no significant differences between groups based on either environmental temperature or precipitation. The AmFV abundance correlated negatively with Bartonella apihabitans, Bartonella choladocola, and positively with Frischella perrara, Gilliamella apicola, Gilliamella sp. ESL0443, Lactobacillus apis, Lactobacillus kullabergensis, Lactobacillus sp. IBH004. De novo metagenome assembly of four samples resulted in almost the complete AmFV genome. According to phylogenetic analysis based on DNA polymerase, the Hungarian strains are closest to the strain CH-05 isolated in Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Zsigmond, Laura AU - Juhász-Erdélyi, Annabella AU - Valkai, Ildikó AU - Aleksza, Dávid AU - Rigó, Gábor AU - Kant, Kamal AU - Szepesi, Ágnes AU - Fiorani, Fabio AU - Körber, Niklas AU - Kovács, László AU - Szabados, László TI - Mitochondrial complex I subunit NDUFS8.2 modulates responses to stresses associated with reduced water availability JF - PLANT PHYSIOLOGY AND BIOCHEMISTRY J2 - PLANT PHYSIOL BIOCH (PPB) VL - 208 PY - 2024 SN - 0981-9428 DO - 10.1016/j.plaphy.2024.108466 UR - https://m2.mtmt.hu/api/publication/34721677 ID - 34721677 LA - English DB - MTMT ER - TY - JOUR AU - Kant, Kamal AU - Rigó, Gábor AU - Faragó, Dóra AU - Benyó, Dániel AU - Tengölics, Roland AU - Szabados, László AU - Zsigmond, Laura TI - Mutation in Arabidopsis mitochondrial Pentatricopeptide repeat 40 gene affects tolerance to water deficit JF - PLANTA J2 - PLANTA VL - 259 PY - 2024 IS - 4 SN - 0032-0935 DO - 10.1007/s00425-024-04354-w UR - https://m2.mtmt.hu/api/publication/34721641 ID - 34721641 N1 - Funding Agency and Grant Number: HUN-REN Biological Research Centre, Szeged Funding text: Open access funding provided by HUN-REN Biological Research Centre, Szeged. LA - English DB - MTMT ER - TY - JOUR AU - Sultana, Most. Sharmin AU - Sakurai, Chisato AU - Biswas, Md. Sanaullah AU - Szabados, László AU - Mano, Jun'ichi TI - Accumulation of reactive carbonyl species in roots as the primary cause of salt stress-induced growth retardation of Arabidopsis thaliana JF - PHYSIOLOGIA PLANTARUM J2 - PHYSIOL PLANTARUM VL - 176 PY - 2024 IS - 1 PG - 10 SN - 0031-9317 DO - 10.1111/ppl.14198 UR - https://m2.mtmt.hu/api/publication/34598013 ID - 34598013 N1 - Funding Agency and Grant Number: Japan Society for the Promotion of Science [17H3700, 20H03278, 21F21383]; KAKENHI grants from the Japan Society for the Promotion of Science Funding text: This work was supported by KAKENHI grants from the Japan Society for the Promotion of Science (17H3700, 20H03278 and 21F21383). AB - Salt stress on plants induces an increase in reactive oxygen species (ROS), which then leads to the formation of reactive carbonyl species (RCS) such as acrolein and 4-hydroxy-(E)-2-nonenal (HNE), potent cytotoxins generated from lipid peroxides. We recently showed that salt-stress treatment of Arabidopsis thaliana plants increased RCS levels, and exogenously added RCS-scavenging chemicals alleviated the stress symptoms, indicating that RCS were responsible for the tissue damage in salt-stressed plants. To obtain deeper insights into the role of RCS in stressed plants, we here analyzed changes in the levels of various RCS in the roots and shoots of A. thaliana. NaCl (90 mM) addition to the culture medium as a salt-stress treatment caused growth inhibition and leaf chlorosis. Carbonyl analysis using HPLC revealed that the stress treatment induced a 2-fold increase in the root levels of RCS, including acrolein, HNE and 4-hydroxy-(E)-2-hexenal (HHE). In the shoots, basal levels and stress-induced increases of the RCS were lower than in roots. In the transgenic A. thaliana plants that overexpress the RCS-scavenging enzyme 2-alkenal reductase (AER) cDNA under the beta-estradiol (beta-ED)-responsive promoter, salt stress induced less damage than in the wild-type under beta-ED supplementation. The AER overexpression suppressed the stress-induced increases in HNE, acrolein, HHE and (E)-2-hexenal in roots and in HNE in leaves, but not the ROS increase. These results suggest that the RCS increase in roots was the primary cause of salt-induced damages. Enhancing RCS-scavenging abilities, such as by AER overexpression, could be a new strategy to confer salt-stress tolerance to plants. LA - English DB - MTMT ER - TY - JOUR AU - Jamwal, Rohit AU - Devi, Pukhrambam Pushpa AU - Rani, Vaishali AU - Rawat, Nitish AU - Daimei, Guisuibou AU - Saurav, Gunjan Kumar AU - Renukadevi, Perumal AU - Yadav, Karuna AU - Rajagopal, Raman TI - Structural and Functional Analysis of Groundnut bud necrosis virus (GBNV) Using Computational and Biochemical Approaches JF - MOLECULAR BIOTECHNOLOGY J2 - MOL BIOTECHNOL PY - 2024 PG - 14 SN - 1073-6085 DO - 10.1007/s12033-024-01046-4 UR - https://m2.mtmt.hu/api/publication/34575642 ID - 34575642 N1 - Funding Agency and Grant Number: University of Delhi; DST-PURSE; UGC Funding text: We thank Ashok Kumar and Ravi Singh for their lab assistance. DST-PURSE and Delhi University-IoE grant funded part of this work. Rohit Jamwal thanks UGC for providing financial support. AB - Groundnut bud necrosis virus (GBNV) belonging to the genus Orthotospovirus is transmitted by its vector Thrips palmi. It is a tri-segmented RNA virus that consists of L, M, and S RNA segments. We analysed the secondary structure features of GBNV proteins through various software and predicted the transmembrane helix, glycosylation, and signal peptidase sites within the GBNV protein sequences (G(N), G(C), N, NSm, and NSs). In glycoprotein sequence, extended strands are predominant (52.87%) whereas the N protein sequence mostly contains alpha helices (47.46%). The random coils are present in movement protein (43.97%) and structural protein (39.41%). We generated the 3D structure of G(N) and N protein using SWISS MODEL software and quality is validated through PROCHECK and PDBsum software. We also expressed the GBNV proteins (G(N), G(C), N, NSm, and NSs) in bacterial expression system. The recombinant proteins were used to raise polyclonal antibodies in mice. Our study will be useful in understanding GBNV protein structures in further detail by analysing the important domains that interact with the thrips proteins. This will further aid us in understanding virus-vector relationship through the application of protein-protein interaction and other immunodiagnostic techniques. LA - English DB - MTMT ER - TY - JOUR AU - Biswas, Avratanu AU - Akhtar, Parveen AU - Lambrev, Petar AU - van, Stokkum Ivo H. M. TI - Energy transfer from phycobilisomes to photosystem I at room temperature JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 14 PY - 2024 PG - 10 SN - 1664-462X DO - 10.3389/fpls.2023.1300532 UR - https://m2.mtmt.hu/api/publication/34575639 ID - 34575639 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Fund [NKFI FK-139067, ANN-144012, 2018-1.2.1-NKP-2018-00009]; Hungarian Research Network [SA-76/2021]; LASERLAB-EUROPE [871124]; European Union Funding text: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by grants from the National Research, Development and Innovation Fund (NKFI FK-139067 to PA and ANN-144012 and 2018-1.2.1-NKP-2018-00009 to PL) and the Hungarian Research Network (SA-76/2021 to PA). The research leading to these results has received funding from LASERLAB-EUROPE (grant agreement no. 871124, European Union's Horizon 2020 research and innovation program). AB - The phycobilisomes function as the primary light-harvesting antennae in cyanobacteria and red algae, effectively harvesting and transferring excitation energy to both photosystems. Here we investigate the direct energy transfer route from the phycobilisomes to photosystem I at room temperature in a mutant of the cyanobacterium Synechocystis sp. PCC 6803 that lacks photosystem II. The excitation dynamics are studied by picosecond time-resolved fluorescence measurements in combination with global and target analysis. Global analysis revealed several fast equilibration time scales and a decay of the equilibrated system with a time constant of approximate to 220 ps. From simultaneous target analysis of measurements with two different excitations of 400 nm (chlorophyll a) and 580 nm (phycobilisomes) a transfer rate of 42 ns-1 from the terminal emitter of the phycobilisome to photosystem I was estimated. LA - English DB - MTMT ER - TY - JOUR AU - Vályi, Péter AU - Wirth, Roland AU - Minárovits, János AU - Strang, Orsolya AU - Maróti, Gergely AU - Kovács, Kornél Lajos TI - The oral microbiome of a family including Papillon-Lefèvre-syndrome patients and clinically healthy members JF - BMC ORAL HEALTH J2 - BMC ORAL HEALTH VL - 24 PY - 2024 IS - 1 PG - 17 SN - 1472-6831 DO - 10.1186/s12903-024-03856-z UR - https://m2.mtmt.hu/api/publication/34558800 ID - 34558800 N1 - Department of Oral Diagnostics, Faculty of Dentistry, Semmelweis University, Szentkirályi u 47, Budapest, H1085, Hungary Department of Biotechnology, University of Szeged, Közép fasor 52, Szeged, H6726, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza L. krt 64, Szeged, H6720, Hungary Institute of Biophysics, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Institute of Plant Biology, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Export Date: 1 May 2024 Correspondence Address: Vályi, P.; Department of Oral Diagnostics, Szentkirályi u 47, Hungary; email: valyi.peter@dent.semmelweis-univ.hu Chemicals/CAS: dipeptidyl peptidase I, 9032-68-2 Funding details: European Regional Development Fund, ERDF, FK123899, GINOP-2.3.2-15-2016-00011, PD132145 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding text 1: This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. Funding text 2: Open access funding provided by Semmelweis University. This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. AB - The oral microbiota composition of patients diagnosed with Papillon-Lefèvre-syndrome and treated for several years were compared to those existing in the oral cavity of the clinically healthy family members and a cohort of patients having various stages of chronic periodontitis. LA - English DB - MTMT ER - TY - JOUR AU - Albert, Pál AU - Varga, Bence AU - Ferenc, Györgyi AU - Kiss, Antal TI - Conversion of the CG specific M.MpeI DNA methyltransferase into an enzyme predominantly methylating CCA and CCC sites JF - NUCLEIC ACIDS RESEARCH J2 - NUCLEIC ACIDS RES VL - 52 PY - 2024 SP - 1896 EP - 1908 PG - 13 SN - 0305-1048 DO - 10.1093/nar/gkad1217 UR - https://m2.mtmt.hu/api/publication/34547384 ID - 34547384 N1 - Funding Agency and Grant Number: Ministry of Finance of Hungary [GINOP-2.3.2-15-2016-00001]; Eotvoes Lorand Research Network (ELKH) [SA-110/2021] Funding text: Ministry of Finance of Hungary [GINOP-2.3.2-15-2016-00001]; Eotvoes Lorand Research Network (ELKH) [SA-110/2021]. Funding for open access charge: Budget of our institute. AB - We used structure guided mutagenesis and directed enzyme evolution to alter the specificity of the CG specific bacterial DNA (cytosine-5) methyltransferase M.MpeI. Methylation specificity of the M.MpeI variants was characterized by digestions with methylation sensitive restriction enzymes and by measuring incorporation of tritiated methyl groups into double-stranded oligonucleotides containing single CC, CG, CA or CT sites. Site specific mutagenesis steps designed to disrupt the specific contacts between the enzyme and the non-substrate base pair of the target sequence (5 '-CG/5 '-CG) yielded M.MpeI variants with varying levels of CG specific and increasing levels of CA and CC specific MTase activity. Subsequent random mutagenesis of the target recognizing domain coupled with selection for non-CG specific methylation yielded a variant, which predominantly methylates CC dinucleotides, has very low activity on CG and CA sites, and no activity on CT sites. This M.MpeI variant contains a one amino acid deletion (Delta A323) and three substitutions (N324G, R326G and E305N) in the target recognition domain. The mutant enzyme has very strong preference for A and C in the 3 ' flanking position making it a CCA and CCC specific DNA methyltransferase. Graphical Abstract LA - English DB - MTMT ER -