TY - JOUR AU - Páli, Tibor AU - Feniouk, Boris AU - Wilkens, Stephan TI - Editorial: Functions, working mechanisms, and regulation of rotary ATPases and Ductin proteins JF - FRONTIERS IN MOLECULAR BIOSCIENCES J2 - FRONT MOL BIOSCI VL - 11 PY - 2024 SN - 2296-889X DO - 10.3389/fmolb.2024.1399421 UR - https://m2.mtmt.hu/api/publication/34812598 ID - 34812598 LA - English DB - MTMT ER - TY - JOUR AU - Faragó, Anna AU - Zvara, Ágnes AU - Tiszlavicz, László AU - Hunyadi-Gulyás Éva, Csilla AU - Darula, Zsuzsanna AU - Hegedűs, Zoltán AU - Szabó, Enikő AU - Surguta, Sára Eszter AU - Tóvári, József AU - Puskás, László AU - Szebeni, Gábor TI - Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model. JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 21 SN - 1661-6596 DO - 10.3390/ijms25074022 UR - https://m2.mtmt.hu/api/publication/34790193 ID - 34790193 N1 - * Megosztott szerzőség AB - A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies. LA - English DB - MTMT ER - TY - JOUR AU - Greipel, Erika AU - Nagy, Krisztina AU - Csákvári, Eszter AU - Dér, László AU - Galajda, Péter AU - Kutasi, József TI - Chemotactic Interactions of Scenedesmus sp. and Azospirillum brasilense Investigated by Microfluidic Methods JF - MICROBIAL ECOLOGY J2 - MICROB ECOL VL - 87 PY - 2024 IS - 1 SN - 0095-3628 DO - 10.1007/s00248-024-02366-3 UR - https://m2.mtmt.hu/api/publication/34749976 ID - 34749976 AB - The use of algae for industrial, biotechnological, and agricultural purposes is spreading globally. Scenedesmus species can play an essential role in the food industry and agriculture due to their favorable nutrient content and plant-stimulating properties. Previous research and the development of Scenedesmus -based foliar fertilizers raised several questions about the effectiveness of large-scale algal cultivation and the potential effects of algae on associative rhizobacteria. In the microbiological practice applied in agriculture, bacteria from the genus Azospirillum are one of the most studied plant growth-promoting, associative, nitrogen-fixing bacteria. Co-cultivation with Azospirillum species may be a new way of optimizing Scenedesmus culturing, but the functioning of the co-culture system still needs to be fully understood. It is known that Azospirillum brasilense can produce indole-3-acetic acid, which could stimulate algae growth as a plant hormone. However, the effect of microalgae on Azospirillum bacteria is unclear. In this study, we investigated the behavior of Azospirillum brasilense bacteria in the vicinity of Scenedesmus sp . or its supernatant using a microfluidic device consisting of physically separated but chemically coupled microchambers. Following the spatial distribution of bacteria within the device, we detected a positive chemotactic response toward the microalgae culture. To identify the metabolites responsible for this behavior, we tested the chemoeffector potential of citric acid and oxaloacetic acid, which, according to our HPLC analysis, were present in the algae supernatant in 0.074 mg/ml and 0.116 mg/ml concentrations, respectively. We found that oxaloacetic acid acts as a chemoattractant for Azospirillum brasilense . LA - English DB - MTMT ER - TY - JOUR AU - Búzás, András AU - Makai, András AU - Groma, Géza AU - Dancsházy, Zsolt AU - Szendi, István AU - Kish, Laszlo B. AU - Santa Maria, Anaraquel AU - Dér, András TI - Hierarchical organization of human physical activity JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-024-56185-0 UR - https://m2.mtmt.hu/api/publication/34737118 ID - 34737118 N1 - Institute of Biophysics, HUN-REN Biological Research Centre, Temesvári Krt. 62, Szeged, 6701, Hungary Department of Psychiatry, Kiskunhalas Semmelweis Hospital, 1 Dr. Monszpart László Street, Kiskunhalas, 6400, Hungary Department of Electrical and Computer Engineering, Texas A & amp;M University, TAMUS 3128, College Station, TX 77843-3128, United States Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, United States Export Date: 19 April 2024 Correspondence Address: Santa-Maria, A.R.; Institute of Biophysics, Temesvári Krt. 62, Hungary; email: anaraquel.santamaria@wyss.harvard.edu Correspondence Address: Dér, A.; Institute of Biophysics, Temesvári Krt. 62, Hungary; email: der.andras@brc.hu AB - Human physical activity (HPA), a fundamental physiological signal characteristic of bodily motion is of rapidly growing interest in multidisciplinary research. Here we report the existence of hitherto unidentified hierarchical levels in the temporal organization of HPA on the ultradian scale: on the minute's scale, passive periods are followed by activity bursts of similar intensity (‘quanta’) that are organized into superstructures on the hours- and on the daily scale. The time course of HPA can be considered a stochastic, quasi-binary process, where quanta, assigned to task-oriented actions are organized into work packages on higher levels of hierarchy. In order to grasp the essence of this complex dynamic behaviour, we established a stochastic mathematical model which could reproduce the main statistical features of real activity time series. The results are expected to provide important data for developing novel behavioural models and advancing the diagnostics of neurological or psychiatric diseases. LA - English DB - MTMT ER - TY - JOUR AU - Laczkó-Dobos, Hajnalka AU - Bhattacharjee, Arindam AU - Maddali, Asha Kiran AU - Kincses, András AU - Abuammar, Hussein AU - Sebőkné Nagy, Krisztina AU - Páli, Tibor AU - Dér, András AU - Hegedűs, Tamás AU - Csordás, Gábor AU - Juhász, Gábor TI - PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion JF - AUTOPHAGY J2 - AUTOPHAGY VL - AiP PY - 2024 PG - 12 SN - 1554-8627 DO - 10.1080/15548627.2024.2322493 UR - https://m2.mtmt.hu/api/publication/34724664 ID - 34724664 N1 - Institute of Genetics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Doctoral School of Biology, University of Szeged, Szeged, Hungary Institute of Biophysics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary HUN-REN Biophysical Virology Research Group, Budapest, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Export Date: 27 March 2024 Correspondence Address: Juhász, G.; HUN-REN Biological Research Centre Szeged, Temesvari krt. 62, Hungary; email: juhasz.gabor@brc.hu LA - English DB - MTMT ER - TY - CHAP AU - Sávai, Gergő AU - Kartali, Tünde AU - Benci, Dániel Attila AU - Patai, Roland AU - Lipinszki, Zoltán AU - Vágvölgyi, Csaba AU - Papp, Tamás TI - Mikovírusok azonosítása Rhizopus fajokban T2 - Biotechnológiai Szakmai Nap Absztraktfüzet PB - Doktoranduszok Országos Szövetsége (DOSZ) CY - Budapest SN - 9786156457448 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34723493 ID - 34723493 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Vágvölgyi, Máté AU - Laczkó, Dávid AU - Santa Maria, Anaraquel AU - Vigh, Judit Piroska AU - Walter, Fruzsina AU - Berkecz, Róbert AU - Deli, Mária Anna AU - Tóth, Gábor AU - Hunyadi, Attila TI - 17-Oxime ethers of oxidized ecdysteroid derivatives modulate oxidative stress in human brain endothelial cells and dose-dependently might protect or damage the blood-brain barrier JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 2 PG - 15 SN - 1932-6203 DO - 10.1371/journal.pone.0290526 UR - https://m2.mtmt.hu/api/publication/34691003 ID - 34691003 N1 - Institute of Pharmacognosy, University of Szeged, Szeged, Hungary Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, United States Doctoral School of Biology, University of Szeged, Szeged, Hungary Institute of Pharmaceutical Analysis, University of Szeged, Szeged, Hungary NMR Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Budapest, Hungary Interdisciplinary Centre of Natural Products, University of Szeged, Szeged, Hungary HUN-REN-SZTE Biologically Active Natural Products Research Group, Szeged, Hungary Export Date: 18 March 2024 CODEN: POLNC Correspondence Address: Hunyadi, A.; Institute of Pharmacognosy, Hungary; email: hunyadi.attila@szte.hu AB - 20-Hydroxyecdysone and several of its oxidized derivatives exert cytoprotective effect in mammals including humans. Inspired by this bioactivity of ecdysteroids, in the current study it was our aim to prepare a set of sidechain-modified derivatives and to evaluate their potential to protect the blood-brain barrier (BBB) from oxidative stress. Six novel ecdysteroids, including an oxime and five oxime ethers, were obtained through regioselective synthesis from a sidechain-cleaved calonysterone derivative 2 and fully characterized by comprehensive NMR techniques revealing their complete 1 H and 13 C signal assignments. Surprisingly, several compounds sensitized hCMEC/D3 brain microvascular endothelial cells to tert -butyl hydroperoxide (tBHP)-induced oxidative damage as recorded by impedance measurements. Compound 8 , containing a benzyloxime ether moiety in its sidechain, was the only one that exerted a protective effect at a higher, 10 μM concentration, while at lower (10 nM– 1 μM) concentrations it promoted tBHP-induced cellular damage. Brain endothelial cells were protected from tBHP-induced barrier integrity decrease by treatment with 10 μM of compound 8 , which also mitigated the intracellular reactive oxygen species production elevated by tBHP. Based on our results, 17-oxime ethers of oxidized ecdysteroids modulate oxidative stress of the BBB in a way that may point towards unexpected toxicity. Further studies are needed to evaluate any possible risk connected to dietary ecdysteroid consumption and CNS pathologies in which BBB damage plays an important role. LA - English DB - MTMT ER - TY - JOUR AU - Deli, Mária Anna AU - Porkoláb, Gergő AU - Kincses, András AU - Mészáros, Mária AU - Szecskó, Anikó AU - Kocsis, Anna AU - Vigh, Judit Piroska AU - Valkai, Sándor AU - Veszelka, Szilvia AU - Walter, Fruzsina AU - Dér, András TI - Lab-on-a-chip models of the blood-brain barrier: evolution, problems, perspectives JF - LAB ON A CHIP J2 - LAB CHIP VL - 24 PY - 2024 IS - 5 SP - 1030 EP - 1063 PG - 34 SN - 1473-0197 DO - 10.1039/d3lc00996c UR - https://m2.mtmt.hu/api/publication/34673907 ID - 34673907 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [K-143766, K-124922, FK-143233, PD-138930, PD-143268]; Hungarian Research Network [SA-111/2021]; Centenarian Foundation; Talentum Foundation of Gedeon Richter Plc. [UNKP-23-3-SZTE-517, UNKP-23-3-SZTE-535]; National Academy of Scientist Education Program of the National Biomedical Foundation under Hungarian Ministry of Culture and Innovation; New National Excellence Program of the Hungarian Ministry of Culture and Innovation; [H-1103]; [19-21]; [UNKP-23-3-SZTE-497] Funding text: The following funding was received from the National Research, Development and Innovation Office, Hungary: grants K-143766 (to M. A. D.), K-124922 (to A. D.), FK-143233 (to S. V.), PD-138930 (to M. M.), PD-143268 (to A. K.). F. R. W. was supported by the grant SA-111/2021 from the Hungarian Research Network. M. M. was supported by the Centenarian Foundation, A. S. and J. P. V. by the Talentum Foundation of Gedeon Richter Plc. (H-1103 Budapest, Gyoemr & odblac;i str. 19-21. Hungary). G. P. was supported by the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation. The New National Excellence Program of the Hungarian Ministry of Culture and Innovation supported G. P. (UNKP-23-3-SZTE-497), A. S. (UNKP-23-3-SZTE-517), and J. P. V. (UNKP-23-3-SZTE-535). LA - English DB - MTMT ER - TY - JOUR AU - Csonti, Katalin AU - Fazakas, Csilla AU - Molnár, Kinga AU - Wilhelm, Imola Mária AU - Krizbai, István Adorján AU - Végh, Attila Gergely TI - Breast adenocarcinoma cells adhere stronger to brain pericytes than to endothelial cells JF - COLLOIDS AND SURFACES B: BIOINTERFACES J2 - COLLOID SURFACE B VL - 234 PY - 2024 SN - 0927-7765 DO - 10.1016/j.colsurfb.2024.113751 UR - https://m2.mtmt.hu/api/publication/34628964 ID - 34628964 LA - English DB - MTMT ER - TY - JOUR AU - Váradi, Györgyi AU - Bende, Gábor AU - Borics, Attila AU - Dán, Kinga AU - Rákhely, Gábor AU - Tóth, Gábor AU - Galgóczi, László Norbert TI - Rational Design of Antifungal Peptides Based on the γ-Core Motif of a Neosartorya (Aspergillus) fischeri Antifungal Protein to Improve Structural Integrity, Efficacy, and Spectrum JF - ACS OMEGA J2 - ACS OMEGA VL - 9 PY - 2024 IS - 6 SP - 7206 EP - 7214 PG - 9 SN - 2470-1343 DO - 10.1021/acsomega.3c09377 UR - https://m2.mtmt.hu/api/publication/34627084 ID - 34627084 N1 - Funding Agency and Grant Number: , Nemzeti Kutat?si Fejleszt?si ?s Innov?ci?s Hivatal [TKP2021-EGA-32]; Hungarian National Research, Development, and Innovation OfficeyNKFIH [FK 134343]; Hungarian National Research Development and Innovation OfficeyNKFIH; University of Szeged Open Access Fund [6653] Funding text: G.V. and G.K.T. were supported by the TKP2021-EGA-32 fund of the Hungarian National Research, Development, and Innovation OfficeyNKFIH. The present work of L.G. was financed by the Hungarian National Research Development and Innovation OfficeyNKFIH, FK 134343 project. The open-access publishing was supported by the University of Szeged Open Access Fund; grant number: 6653. LA - English DB - MTMT ER -