@article{MTMT:34812598, title = {Editorial: Functions, working mechanisms, and regulation of rotary ATPases and Ductin proteins}, url = {https://m2.mtmt.hu/api/publication/34812598}, author = {Páli, Tibor and Feniouk, Boris and Wilkens, Stephan}, doi = {10.3389/fmolb.2024.1399421}, journal-iso = {FRONT MOL BIOSCI}, journal = {FRONTIERS IN MOLECULAR BIOSCIENCES}, volume = {11}, unique-id = {34812598}, year = {2024}, eissn = {2296-889X}, orcid-numbers = {Páli, Tibor/0000-0003-1649-1097} } @article{MTMT:34790193, title = {Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model.}, url = {https://m2.mtmt.hu/api/publication/34790193}, author = {Faragó, Anna and Zvara, Ágnes and Tiszlavicz, László and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Hegedűs, Zoltán and Szabó, Enikő and Surguta, Sára Eszter and Tóvári, József and Puskás, László and Szebeni, Gábor}, doi = {10.3390/ijms25074022}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {25}, unique-id = {34790193}, issn = {1661-6596}, abstract = {A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies.}, keywords = {colorectal carcinoma; lectin binding sugar code; proteomic analysis of murine CRC}, year = {2024}, eissn = {1422-0067}, orcid-numbers = {Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632} } @article{MTMT:34749976, title = {Chemotactic Interactions of Scenedesmus sp. and Azospirillum brasilense Investigated by Microfluidic Methods}, url = {https://m2.mtmt.hu/api/publication/34749976}, author = {Greipel, Erika and Nagy, Krisztina and Csákvári, Eszter and Dér, László and Galajda, Péter and Kutasi, József}, doi = {10.1007/s00248-024-02366-3}, journal-iso = {MICROB ECOL}, journal = {MICROBIAL ECOLOGY}, volume = {87}, unique-id = {34749976}, issn = {0095-3628}, abstract = {The use of algae for industrial, biotechnological, and agricultural purposes is spreading globally. Scenedesmus species can play an essential role in the food industry and agriculture due to their favorable nutrient content and plant-stimulating properties. Previous research and the development of Scenedesmus -based foliar fertilizers raised several questions about the effectiveness of large-scale algal cultivation and the potential effects of algae on associative rhizobacteria. In the microbiological practice applied in agriculture, bacteria from the genus Azospirillum are one of the most studied plant growth-promoting, associative, nitrogen-fixing bacteria. Co-cultivation with Azospirillum species may be a new way of optimizing Scenedesmus culturing, but the functioning of the co-culture system still needs to be fully understood. It is known that Azospirillum brasilense can produce indole-3-acetic acid, which could stimulate algae growth as a plant hormone. However, the effect of microalgae on Azospirillum bacteria is unclear. In this study, we investigated the behavior of Azospirillum brasilense bacteria in the vicinity of Scenedesmus sp . or its supernatant using a microfluidic device consisting of physically separated but chemically coupled microchambers. Following the spatial distribution of bacteria within the device, we detected a positive chemotactic response toward the microalgae culture. To identify the metabolites responsible for this behavior, we tested the chemoeffector potential of citric acid and oxaloacetic acid, which, according to our HPLC analysis, were present in the algae supernatant in 0.074 mg/ml and 0.116 mg/ml concentrations, respectively. We found that oxaloacetic acid acts as a chemoattractant for Azospirillum brasilense .}, year = {2024}, eissn = {1432-184X} } @article{MTMT:34737118, title = {Hierarchical organization of human physical activity}, url = {https://m2.mtmt.hu/api/publication/34737118}, author = {Búzás, András and Makai, András and Groma, Géza and Dancsházy, Zsolt and Szendi, István and Kish, Laszlo B. and Santa Maria, Anaraquel and Dér, András}, doi = {10.1038/s41598-024-56185-0}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34737118}, issn = {2045-2322}, abstract = {Human physical activity (HPA), a fundamental physiological signal characteristic of bodily motion is of rapidly growing interest in multidisciplinary research. Here we report the existence of hitherto unidentified hierarchical levels in the temporal organization of HPA on the ultradian scale: on the minute's scale, passive periods are followed by activity bursts of similar intensity (‘quanta’) that are organized into superstructures on the hours- and on the daily scale. The time course of HPA can be considered a stochastic, quasi-binary process, where quanta, assigned to task-oriented actions are organized into work packages on higher levels of hierarchy. In order to grasp the essence of this complex dynamic behaviour, we established a stochastic mathematical model which could reproduce the main statistical features of real activity time series. The results are expected to provide important data for developing novel behavioural models and advancing the diagnostics of neurological or psychiatric diseases.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Santa Maria, Anaraquel/0000-0003-3505-5477} } @article{MTMT:34724664, title = {PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion}, url = {https://m2.mtmt.hu/api/publication/34724664}, author = {Laczkó-Dobos, Hajnalka and Bhattacharjee, Arindam and Maddali, Asha Kiran and Kincses, András and Abuammar, Hussein and Sebőkné Nagy, Krisztina and Páli, Tibor and Dér, András and Hegedűs, Tamás and Csordás, Gábor and Juhász, Gábor}, doi = {10.1080/15548627.2024.2322493}, journal-iso = {AUTOPHAGY}, journal = {AUTOPHAGY}, volume = {AiP}, unique-id = {34724664}, issn = {1554-8627}, year = {2024}, eissn = {1554-8635}, orcid-numbers = {Páli, Tibor/0000-0003-1649-1097; Hegedűs, Tamás/0000-0002-0331-9629; Csordás, Gábor/0000-0001-6871-6839; Juhász, Gábor/0000-0001-8548-8874} } @{MTMT:34723493, title = {Mikovírusok azonosítása Rhizopus fajokban}, url = {https://m2.mtmt.hu/api/publication/34723493}, author = {Sávai, Gergő and Kartali, Tünde and Benci, Dániel Attila and Patai, Roland and Lipinszki, Zoltán and Vágvölgyi, Csaba and Papp, Tamás}, booktitle = {Biotechnológiai Szakmai Nap Absztraktfüzet}, unique-id = {34723493}, year = {2024}, orcid-numbers = {Lipinszki, Zoltán/0000-0002-2067-0832; Vágvölgyi, Csaba/0000-0003-0009-7773; Papp, Tamás/0000-0001-8211-5431} } @article{MTMT:34691003, title = {17-Oxime ethers of oxidized ecdysteroid derivatives modulate oxidative stress in human brain endothelial cells and dose-dependently might protect or damage the blood-brain barrier}, url = {https://m2.mtmt.hu/api/publication/34691003}, author = {Vágvölgyi, Máté and Laczkó, Dávid and Santa Maria, Anaraquel and Vigh, Judit Piroska and Walter, Fruzsina and Berkecz, Róbert and Deli, Mária Anna and Tóth, Gábor and Hunyadi, Attila}, doi = {10.1371/journal.pone.0290526}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {19}, unique-id = {34691003}, issn = {1932-6203}, abstract = {20-Hydroxyecdysone and several of its oxidized derivatives exert cytoprotective effect in mammals including humans. Inspired by this bioactivity of ecdysteroids, in the current study it was our aim to prepare a set of sidechain-modified derivatives and to evaluate their potential to protect the blood-brain barrier (BBB) from oxidative stress. Six novel ecdysteroids, including an oxime and five oxime ethers, were obtained through regioselective synthesis from a sidechain-cleaved calonysterone derivative 2 and fully characterized by comprehensive NMR techniques revealing their complete 1 H and 13 C signal assignments. Surprisingly, several compounds sensitized hCMEC/D3 brain microvascular endothelial cells to tert -butyl hydroperoxide (tBHP)-induced oxidative damage as recorded by impedance measurements. Compound 8 , containing a benzyloxime ether moiety in its sidechain, was the only one that exerted a protective effect at a higher, 10 μM concentration, while at lower (10 nM– 1 μM) concentrations it promoted tBHP-induced cellular damage. Brain endothelial cells were protected from tBHP-induced barrier integrity decrease by treatment with 10 μM of compound 8 , which also mitigated the intracellular reactive oxygen species production elevated by tBHP. Based on our results, 17-oxime ethers of oxidized ecdysteroids modulate oxidative stress of the BBB in a way that may point towards unexpected toxicity. Further studies are needed to evaluate any possible risk connected to dietary ecdysteroid consumption and CNS pathologies in which BBB damage plays an important role.}, year = {2024}, eissn = {1932-6203}, orcid-numbers = {Vágvölgyi, Máté/0000-0002-2233-9422; Santa Maria, Anaraquel/0000-0003-3505-5477; Walter, Fruzsina/0000-0001-8145-2823; Berkecz, Róbert/0000-0002-9076-2177; Deli, Mária Anna/0000-0001-6084-6524; Hunyadi, Attila/0000-0003-0074-3472} } @article{MTMT:34673907, title = {Lab-on-a-chip models of the blood-brain barrier: evolution, problems, perspectives}, url = {https://m2.mtmt.hu/api/publication/34673907}, author = {Deli, Mária Anna and Porkoláb, Gergő and Kincses, András and Mészáros, Mária and Szecskó, Anikó and Kocsis, Anna and Vigh, Judit Piroska and Valkai, Sándor and Veszelka, Szilvia and Walter, Fruzsina and Dér, András}, doi = {10.1039/d3lc00996c}, journal-iso = {LAB CHIP}, journal = {LAB ON A CHIP}, volume = {24}, unique-id = {34673907}, issn = {1473-0197}, year = {2024}, eissn = {1473-0189}, pages = {1030-1063}, orcid-numbers = {Deli, Mária Anna/0000-0001-6084-6524; Valkai, Sándor/0000-0001-8479-8141; Walter, Fruzsina/0000-0001-8145-2823} } @article{MTMT:34628964, title = {Breast adenocarcinoma cells adhere stronger to brain pericytes than to endothelial cells}, url = {https://m2.mtmt.hu/api/publication/34628964}, author = {Csonti, Katalin and Fazakas, Csilla and Molnár, Kinga and Wilhelm, Imola Mária and Krizbai, István Adorján and Végh, Attila Gergely}, doi = {10.1016/j.colsurfb.2024.113751}, journal-iso = {COLLOID SURFACE B}, journal = {COLLOIDS AND SURFACES B: BIOINTERFACES}, volume = {234}, unique-id = {34628964}, issn = {0927-7765}, year = {2024}, eissn = {1873-4367}, orcid-numbers = {Fazakas, Csilla/0000-0001-7822-5881; Wilhelm, Imola Mária/0000-0003-2366-7337; Végh, Attila Gergely/0000-0003-0524-0946} } @article{MTMT:34627084, title = {Rational Design of Antifungal Peptides Based on the γ-Core Motif of a Neosartorya (Aspergillus) fischeri Antifungal Protein to Improve Structural Integrity, Efficacy, and Spectrum}, url = {https://m2.mtmt.hu/api/publication/34627084}, author = {Váradi, Györgyi and Bende, Gábor and Borics, Attila and Dán, Kinga and Rákhely, Gábor and Tóth, Gábor and Galgóczi, László Norbert}, doi = {10.1021/acsomega.3c09377}, journal-iso = {ACS OMEGA}, journal = {ACS OMEGA}, volume = {9}, unique-id = {34627084}, issn = {2470-1343}, year = {2024}, eissn = {2470-1343}, pages = {7206-7214}, orcid-numbers = {Váradi, Györgyi/0000-0001-7907-8908; Rákhely, Gábor/0000-0003-2557-3641; Tóth, Gábor/0000-0002-3604-4385; Galgóczi, László Norbert/0000-0002-6976-8910} }